Abstract：
: Salvia miltiorrhiza contains two groups of biologically active secondary metabolites termed phenolic compounds (e.g. rosmarinic acid) and tanshinones (e.g. cryptotanshinone). Their roles in plant defense responses were examined using a simplified system consisting of a yeast elicitor and a Ti C58 transformed S. miltiorrhiza cell line. Both dosage and time course studies were carried out on the effects of yeast elicitor on the formation of rosmarinic acid and cryptotanshinone. It was found that the yeast elicitor reduced the constituent level of rosmarinic acid (from ca. 5% to ca. 3.0% of dry cell weight) whereas the level of cryptotanshinone was enhanced greatly (from a negligible amount to ca. 20 mg/l). These results suggest that in S. miltiorrhiza, rosmarinic acid and cryptotanshinone may take part in plant passive and active defense responses, respectively, against pathogen attack. Cryptotanshinone was identified as a phytoalexin in S. miltiorrhiza for the first time. Results of the treatment of cell cultures with 2-aminoindan-2-phosphonic acid, a highly specific and potent inhibitor of phenylalanine ammonia-lyase (EC 4.3.1.5), indicated that this compound did not inhibit yeast elicitor induced tanshinone formation, but did inhibit rosmarinic acid biosynthesis.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Chen H,Chen Fdoi
10.1007/s002999900166subject
Has Abstractpub_date
2000-06-01 00:00:00pages
710-717issue
7eissn
0721-7714issn
1432-203Xpii
10.1007/s002999900166journal_volume
19pub_type
杂志文章abstract：KEY MESSAGE:An inorganic pyrophosphorylase gene, ThPP1 , modulated the accumulations of phosphate and osmolytes by up-regulating the differentially expression genes, thus enhancing the tolerance of the transgenic rice to alkali stress (AS). Inorganic pyrophosphorylase is essential in catalyzing the hydrolysis of pyroph...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-017-2208-y
更新日期：2017-12-01 00:00:00
abstract：:Developmental biology studies in general benefit from model organisms that are well characterized. Arabidopsis thaliana fulfills this criterion and represents one of the best experimental systems to study developmental processes in higher plants. Light is a crucial factor that drives photosynthesis, but that also regu...
journal_title：Plant cell reports
pub_type： 杂志文章,评审
doi：10.1007/s00299-014-1591-x
更新日期：2014-05-01 00:00:00
abstract：:Cell suspension cultures of cowpea (Vigna sp.) were able to produce extracellular peroxidase. Different growth regulator concentrations induced different peroxidase activity in callus. The crude extracellular medium after four weeks of culture showed higher (6 times) specific peroxidase activity and higher thermo stab...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232092
更新日期：1990-07-01 00:00:00
abstract：:Phenylalanine ammonia-lyase [PAL, EC 4.3.1.24 (formerly EC 4.3.1.5)], functions in the plant phenylpropanoid biosynthetic pathway to deaminate the amino acid L-phenylalanine forming trans-cinnamic acid and ammonia. The human inherited metabolic disorder phenylketonuria (PKU) is characterized by an inability of individ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-008-0650-6
更新日期：2009-03-01 00:00:00
abstract：KEY MESSAGE:Oral administration of maize-expressed H3N2 nucleoprotein induced antibody responses in mice showing the immunogenicity of plant-derived antigen and its potential to be utilized as a universal flu vaccine. Influenza A viruses cause influenza epidemics that are devastating to humans and livestock. The vaccin...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-015-1758-0
更新日期：2015-06-01 00:00:00
abstract：:The nucleotide sequence of the cucumber (Cucumis sativus L. cv. Baekmibaekdadagi) chloroplast genome was completed. The circular double-stranded DNA, consisting of 155,527 bp, contained a pair of inverted repeat regions (IRa and IRb) of 25,187 bp each, which were separated by small and large single copy regions of 86,...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-005-0097-y
更新日期：2006-04-01 00:00:00
abstract：:This study aimed at establishing a protocol to increase the number of regenerated shoots and to limit the recovery of "escapes" during the regeneration of transgenic flax plants (cv Barbara). Here, we describe how light, adapted media and selection scheme could stimulate the transformation process, the organogenic pot...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-008-0638-2
更新日期：2009-03-01 00:00:00
abstract：KEY MESSAGE:MicroRNAs have higher expression stability than protein-coding genes in B. napus seeds and are therefore good reference genes for miRNA and mRNA RT-qPCR analysis. Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) has become the "gold standard" to gain insight into function of ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-015-1773-1
更新日期：2015-07-01 00:00:00
abstract：:Leaf explants ofKalanchoe laciniata were cocultivated for different days (2, 4, 6 and 8 days) with disarmedAgrobacterium tumefaciens strains A208SE, GV3111SE and EHA101 carring a binary vector pROA93. The vector contains a cauliflower mosaic virus 35S promotor which drives the coding sequence of neomycin phosphotransf...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00716668
更新日期：1989-06-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-011-1006-1
更新日期：2011-06-01 00:00:00
abstract：:A biolistic particle delivery system was used to genetically transform embryogenic tissue of Pinus radiata. The introduced DNA contained a uidA reporter gene under the control of either the tandem CaMV 35S or the artificial Emu promoter, and the npt II selectable marker controlled by the CaMV 35S promoter. The average...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050426
更新日期：1998-04-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-015-1752-6
更新日期：2015-06-01 00:00:00
abstract：:The Golgi apparatus in plants is organized as a multitude of individual stacks that are motile in the cytoplasm and in close association with the endoplasmic reticulum (ER) (Boevink et al. in Plant J 15:441-447, 1998). These stacks operate as a sorting centre for cargo molecules, providing modification and redirection...
journal_title：Plant cell reports
pub_type： 杂志文章,评审
doi：10.1007/s00299-007-0390-z
更新日期：2007-09-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-008-0579-9
更新日期：2008-11-01 00:00:00
abstract：KEY MESSAGE:The oil palm EgPAL1 gene promoter and its regulatory region were functional as a promoter in the heterologous system of Arabidopsis according to the cis-acting elements present in that region. The promoter was developmentally regulated, vascular tissue specific and responsive to water stress agents. Phenyla...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-017-2228-7
更新日期：2018-02-01 00:00:00
abstract：:We describe a simple and efficient protocol for regeneration-transformation of two diploid Medicago lines: the annual M. truncatula R108-1(c3) and the perennial M. sativa ssp. falcata (L.) Arcangeli PI.564263 selected previously as highly embryogenic genotypes. Here, embryo regeneration of R108-1 to complete plants wa...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050405
更新日期：1998-03-01 00:00:00
abstract：KEY MESSAGE:A plant-derived 0.3 kb constitutive promoter was obtained from AtTCTP expression analysis, and successfully applied to the expression of a selectable marker gene for production of transgenic creeping bentgrass plants. The isolation and use of an efficient promoter is essential to develop a vector system for...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-014-1705-5
更新日期：2015-02-01 00:00:00
abstract：:The study used Actinidia deliciosa endosperm-derived callus to investigate aspects of the morphology, histology and chemistry of extracellular matrix (ECM) structures in morphogenically stable tissue from long-term culture. SEM showed ECM as a membranous layer or reticulated fibrillar and granular structure linking th...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-008-0534-9
更新日期：2008-07-01 00:00:00
abstract：:Protoplasts were isolated from seedling roots, hypocotyls, and cotyledons of four cultivars of Helianthus annuus and from leaves of axenic shoot cultures of the wild species H. praecox, H. scaberimus and H. rigidus. Optimal culture conditions were established for the respective protoplast systems, using the agarose be...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269815
更新日期：1986-08-01 00:00:00
abstract：:The aim of this study was the improvement of embryo production in wheat anther culture. Three butanol alcohols, n-butanol, sec-butanol and tert-butanol, were evaluated for their effect on microspore embryogenesis in two spring cultivars of wheat, Pavon and Caramba. Application of n-butanol, at 0.1 and 0.2% (v/v) in th...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-007-0500-y
更新日期：2008-05-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-010-0852-6
更新日期：2010-06-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-008-0594-x
更新日期：2008-11-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00235258
更新日期：1992-03-01 00:00:00
abstract：:Cultured Catharanthus roseus cells exhibit transmembrane ferricyanide reduction through a plasma membrane redox system which may be associated with proton translocation. Evidence shows that endogenous pyridine nucleotides serve as hydrogen donors for the reaction. The proton translocating function of the redox system ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269886
更新日期：1985-12-01 00:00:00
abstract：:Protoplasts isolated from embryogenic callus cultures derived from immature embryos ofZea mays L. are suitable for analysis of transient gene expression using electroporation-mediated DNA transfer. Expression of introduced genes is comparable to the levels obtained with protoplasts from Black Mexican Sweet suspension ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00716827
更新日期：1989-03-01 00:00:00
abstract：: The influences of light conditions, sucrose and ethylene on in vitro formation and storability of onion (Allium cepa L.) bulblets were studied in various accessions. Light, sucrose and ethylene influenced bulb formation. Storability was primarily enhanced by a high sucrose concentration (100 g/l) in the culture mediu...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990000295
更新日期：2001-02-01 00:00:00
abstract：:The expression of three genes that encode proteins involved in peroxisome biogenesis, beta-oxidation and the glyoxylate cycle was studied in Arabidopsis plants by fusing their promoter regions to the reporter gene luciferase. Malate synthase showed an extremely restricted pattern of expression, being detected only in ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-004-0879-7
更新日期：2005-02-01 00:00:00
abstract：:A new method of estimating the amount of berberine released from minute cell colonies of Thalictrum minus has been devised to facilitate the selection of high berberine-producing cell lines. In this system, cell aggregates obtained from a cell suspension culture are grown on small pieces of an agar culture medium and ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00268477
更新日期：1987-06-01 00:00:00
abstract：:The transit peptide sequence of ibAGP2 (TP2) was found to be capable of targeting protein into the chloroplast in the Arabidopsis protoplasts. TP2 was fused to a beta-glucuronidase (GUS) reporter gene and expressed in Arabidopsis under the control of the ibAGP2 promoter with the aim of dissecting the effect of the tra...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-008-0563-4
更新日期：2008-08-01 00:00:00
abstract：:Cell structures were compared between alkaloid-producing and non-producing cell cultures of Coptis japonica and Thalictrum minus by electron microscopic observation. In alkaloid-producing cells of C. japonica, prior to the onset of alkaloid synthesis, the vacuoles showed a greater volume than in non-producing cells. T...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269721
更新日期：1986-02-01 00:00:00
