Abstract:
:A new method of estimating the amount of berberine released from minute cell colonies of Thalictrum minus has been devised to facilitate the selection of high berberine-producing cell lines. In this system, cell aggregates obtained from a cell suspension culture are grown on small pieces of an agar culture medium and the concentration of berberine which has been released from the cells into the agar piece is assayed by the antibacterial activity against Bacillus cereus MT2026. Screening of 1000 cell colonies by the "agar piece method" has resulted in the isolation of four, high berberine-producing cell lines, although they have been found to be more or less unstable with respect to the biosynthetic capability during successive subcultures.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Suzuki T,Yoshioka T,Hara Y,Tabata M,Fujita Ydoi
10.1007/BF00268477subject
Has Abstractpub_date
1987-06-01 00:00:00pages
194-6issue
3eissn
0721-7714issn
1432-203Xjournal_volume
6pub_type
杂志文章abstract::Putrescine, spermidine, and spermine levels during somatic embryogenesis of interior spruce (Picea glauca x Picea engelmannii complex) were quantified On abscisic acid supplemented growth medium putrescine and spermidine levels increased two-fold coinciding with maturation of the early somatic embryos to globular embr...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232981
更新日期:1996-03-01 00:00:00
abstract::Self-incompatibility (SI) has been studied extensively at the molecular level in Solanaceae, Rosaceae and Scrophulariaceae, all of which exhibit gametophytic self-incompatibility (GSI). In the present study, four PpsS-haplotypes (Prunus pseudocerasus S-haplotypes) comprising at least two genes, i.e., PpsS-RNase (P. ps...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-008-0528-7
更新日期:2008-06-01 00:00:00
abstract::Cultivation of Catharanthus roseus hairy root cultures at different temperatures was found to have an effect on growth rate and indole alkaloid content as well as lipid composition. When lowering the temperature, the roots responded by increasing the degree of unsaturation of cellular lipids, which was mainly due to a...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00234368
更新日期:1992-07-01 00:00:00
abstract::We developed an efficient plant regeneration system from protoplasts for poplar (Populus alba L.). Protoplasts were isolated from 4-day-old suspension cultures derived from seed-induced calli with a yield of 6.96× 106 cells/g fresh weight cells and then cultured at a concentration of 2.5×105 cells/ml in NH4NO3-free Mu...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050378
更新日期:1998-01-01 00:00:00
abstract:KEY MESSAGE:An inorganic pyrophosphorylase gene, ThPP1 , modulated the accumulations of phosphate and osmolytes by up-regulating the differentially expression genes, thus enhancing the tolerance of the transgenic rice to alkali stress (AS). Inorganic pyrophosphorylase is essential in catalyzing the hydrolysis of pyroph...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-017-2208-y
更新日期:2017-12-01 00:00:00
abstract:KEY MESSAGE:Cadmium sensitivity in sultr1;1 - sultr1;2 double mutant with limiting sulfate supply is attributed to the decreased glutathione content that affected oxidative defense but not phytochelatins' synthesis. In plants, glutathione (GSH) homeostasis plays pivotal role in cadmium (Cd) detoxification. GSH is synth...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-015-1892-8
更新日期:2016-02-01 00:00:00
abstract::The ample supply of O2 proved to be of great importance for berberine production in cell suspension culture ofThalictrum minus, as the specific O2 consumption rate of berberine-producing cells was twice as high as that of non-producing cells. Furthermore, berberine yield increased with increases in the volumetric O2 t...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00778546
更新日期:1989-04-01 00:00:00
abstract:KEY MESSAGE:The first Good Manufacturing Practices production of a purification-free rice-based oral cholera vaccine (MucoRice-CTB) from transgenic plants in a closed cultivation system yielded a product meeting regulatory requirements. Despite our knowledge of their advantages, plant-based vaccines remain unavailable ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-015-1911-9
更新日期:2016-03-01 00:00:00
abstract::The influence of light on the expression of NADPH: protochlorophyllide oxidoreductase has been studied in different plant species. The presumptive precursors to this enzyme have been characterized by in vitro translation of poly (A) RNA and immunoprecipitation. Two bands of apparent molecular weights of about 42 000 a...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269229
更新日期:1983-02-01 00:00:00
abstract::A biolistic particle delivery system was used to genetically transform embryogenic tissue of Pinus radiata. The introduced DNA contained a uidA reporter gene under the control of either the tandem CaMV 35S or the artificial Emu promoter, and the npt II selectable marker controlled by the CaMV 35S promoter. The average...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050426
更新日期:1998-04-01 00:00:00
abstract::An improved protocol forAgrobacterium-mediated transformation of the tomato cultivar Moneymaker was developed by examining the effects of six different factors on the efficiency of transformation. Explant size, explant orientation, gelling agent and plate sealant were found to affect transformation efficiency. Two oth...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF01890875
更新日期:1996-12-01 00:00:00
abstract:KEY MESSAGE:Autotetraploid Arabidopsis line esd and 4COL exhibit enhanced tolerance to Cu stress by enhancing activation of antioxidative defenses, altering expression of genes related to Cu transport, chelation, and ABA-responsive. Autopolyploidy is ubiquitous among angiosperms and often results in better adaptation t...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-017-2176-2
更新日期:2017-10-01 00:00:00
abstract::Using a conventional sexual crossing technique, Nicotiana tabacum x N. acuminata was not produced. After the fertilized ovules were cultured for 20 days in a liquid Nitsch H medium, germination was observed. The roots grew rapidly but leaves did not. However, plantlets were produced in an H medium containing Benzylade...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00268613
更新日期:1986-10-01 00:00:00
abstract::In Materials and method section, a sweetpotato variety "Taizhong-6" (China national number 2013003) should be renamed as Ayamurasaki". ...
journal_title:Plant cell reports
pub_type: 杂志文章,已发布勘误
doi:10.1007/s00299-019-02487-x
更新日期:2020-01-01 00:00:00
abstract::Anthocyanin is the primary pigment contributing to red, violet, and blue flower color formation. The solubility of anthocyanins is enhanced by UDP glucose: flavonoid 3-O-glucosyltransferase (UFGT) through transfer of the glucosyl moiety from UDP-glucose to 3-hydroxyl group to produce the first stable pigments. To asse...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1006-1
更新日期:2011-06-01 00:00:00
abstract::We have previously developed procedures for the efficient production of sweet orange (Citrus sinensis L. Osbeck) and Carrizo citrange (C. sinensis L. Osbeck×Poncirus trifoliata L. Raf.) transgenic plants using an Agrobacterium tumefaciens-mediated transformation and shoot tip grafting in vitro regeneration system. We ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050311
更新日期:1997-09-01 00:00:00
abstract::To develop an alternative genetic transformation system that is not dependent on an antibiotic selection strategy, the phosphomannose isomerase gene (pmi) system was evaluated for producing transgenic plants of chickpea (Cicer arietinum L.). A shoot morphogenesis protocol based on the thidiazuron (TDZ)-induced shoot m...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-009-0766-3
更新日期:2009-11-01 00:00:00
abstract:KEY MESSAGE:In GhCFE5 homoeologs, GhCFE5D interacted with more actin homologs and stronger interaction activity than GhCFE5A. GhCFE5D - but not GhCFE5A -overexpression severely disrupted actin cytoskeleton organization and significantly suppressed cell elongation. Homoeologous genes are common in polyploid plants; howe...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-015-1928-0
更新日期:2016-04-01 00:00:00
abstract::Diuraphis noxia (Russian wheat aphid, RWA) is a major pest on wheat in South Africa and most other wheat growing countries. Being a probing-sucking insect, RWAs insert their stylets into the phloem sieve elements and feed on the phloem sap. This feeding causes necrotic lesions in resistant varieties, or decoloration o...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-005-0001-9
更新日期:2006-02-01 00:00:00
abstract::Mitogen-activated protein kinase (MAPK) signaling cascades are evolutionarily conserved fundamental signal transduction pathways. A MAPK cascade consists of many distinct MAPKKK-MAPKK-MAPK modules linked to various upstream receptors and downstream targets through sequential phosphorylation and activation of the casca...
journal_title:Plant cell reports
pub_type: 杂志文章,评审
doi:10.1007/s00299-013-1437-y
更新日期:2013-06-01 00:00:00
abstract::Various inhibitors of polyamine biosynthesis were used to study the role of polyamines in DNA synthesis and cell division in suspension cultures of Catharanthus roseus (L.) G. Don. Arginine decarboxylase (ADC; EC 4.1.1.19) was the major enzyme responsible for putrescine production. DL α-difluoromethylarginine inhibite...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232042
更新日期:1991-06-01 00:00:00
abstract::We previously reported that Arabidopsis indole-3-acetic acid (IAA)-methyltransferase-1 (IAMT1) catalyzes the conversion of IAA, an essential phytohormone, to methyl-IAA (MeIAA) and that IAMT1 plays an important role in leaf development. Here, we present the possible mechanisms of action of MeIAA in Arabidopsis. We sho...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-007-0458-9
更新日期:2008-03-01 00:00:00
abstract::A new freezer stock of an Agrobacterium tumefaciens clone of LBA4404/pBI121, designated 8999, was found to contain a mutation in the T-DNA region. GUS activity in Agrobacterium 8999 was reduced to levels in negative controls of LBA4404. Additionally, GUS activity in T1 seedlings from tobacco plants transformed with 89...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232066
更新日期:1996-02-01 00:00:00
abstract::Plants have developed diverse molecular mechanisms to resist viruses. RNA silencing plays a dominant role in antiviral defense. Recent studies have correlated plant antiviral silencing to epigenetic modification in genomic DNA and protein by remodeling the expression levels of coding genes. The plant host methylation ...
journal_title:Plant cell reports
pub_type: 杂志文章,评审
doi:10.1007/s00299-019-02414-0
更新日期:2019-09-01 00:00:00
abstract::An efficient method was developed using floating membrane rafts (Liferaft(™)) for the micropropagation of Aconitum napellus (Ranunculaceae), a cut flower crop with a low natural propagation rate. This was achieved by introducing shoot tips into culture on Murashige and Skoog's (1962) solid medium, or liquid medium-sup...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00238594
更新日期:1995-03-01 00:00:00
abstract::Sugarcane cell suspensions were initiated from leaf callus and sub-cultured every 7 to 10 days by alternate transfer to MS based medium with 3.0 and 1.0 mg 1(-1)2,4-D. Suspensions older than 3 months gave the most reproducible yields of protoplasts. Isolated protoplasts required 50 mM Ca(2+) in the washing solution an...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269934
更新日期:1988-08-01 00:00:00
abstract::Cell suspension cultures of cowpea (Vigna sp.) were able to produce extracellular peroxidase. Different growth regulator concentrations induced different peroxidase activity in callus. The crude extracellular medium after four weeks of culture showed higher (6 times) specific peroxidase activity and higher thermo stab...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232092
更新日期:1990-07-01 00:00:00
abstract:KEY MESSAGE:Transcript profiling during the early induction phase of oil palm tissue culture and RNAi studies in a model somatic embryogenesis system showed that EgENOD93 expression is essential for somatic embryogenesis. Micropropagation of oil palm through tissue culture is vital for the generation of superior and un...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-020-02571-7
更新日期:2020-11-01 00:00:00
abstract::Efficient protoplast culture and plant regeneration of five U.S. rice cultivars (Oryza sativa L.) - Mercury, Lacassine, Maybelle, Cypress, and Lemont - were obtained from suspension cells maintained in modified General Medium. Embryogenic suspension cells were developed from calli grown on the original callus inductio...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF01690249
更新日期:1995-01-01 00:00:00
abstract:: The influences of light conditions, sucrose and ethylene on in vitro formation and storability of onion (Allium cepa L.) bulblets were studied in various accessions. Light, sucrose and ethylene influenced bulb formation. Storability was primarily enhanced by a high sucrose concentration (100 g/l) in the culture mediu...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990000295
更新日期:2001-02-01 00:00:00