Improvement of Aconitum napellus micropropagation by liquid culture on floating membrane rafts.

Abstract:

:An efficient method was developed using floating membrane rafts (Liferaft(™)) for the micropropagation of Aconitum napellus (Ranunculaceae), a cut flower crop with a low natural propagation rate. This was achieved by introducing shoot tips into culture on Murashige and Skoog's (1962) solid medium, or liquid medium-supported rafts, supplemented by different levels of benzyl adenine (BA). Optimum shoot proliferation on solid medium required 4mg/l BA, whereas for expiants supported on rafts optimal proliferation was achieved at 0.25mg/l BA. Maximum shoot proliferation was found using the floating rafts (propagation ratio of 4.2 per month), 45% higher than the maximum value on solid medium. A similar value could be obtained on solid medium after a period of 2 months. The optimal response to BA was similar for fresh weight gain and shoot length. Growth in a shallow layer of liquid in shake flasks gives a similar shoot multiplication rate to that on floating rafts; however, submerged leaves brown and die.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Watad AA,Kochba M,Nissim A,Gaba V

doi

10.1007/BF00238594

subject

Has Abstract

pub_date

1995-03-01 00:00:00

pages

345-8

issue

6

eissn

0721-7714

issn

1432-203X

journal_volume

14

pub_type

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