Abstract：
KEY MESSAGE:The first Good Manufacturing Practices production of a purification-free rice-based oral cholera vaccine (MucoRice-CTB) from transgenic plants in a closed cultivation system yielded a product meeting regulatory requirements. Despite our knowledge of their advantages, plant-based vaccines remain unavailable for human use in both developing and industrialized countries. A leading, practical obstacle to their widespread use is producing plant-based vaccines that meet governmental regulatory requirements. Here, we report the first production according to current Good Manufacturing Practices of a rice-based vaccine, the cholera vaccine MucoRice-CTB, at an academic institution. To this end, we established specifications and methods for the master seed bank (MSB) of MucoRice-CTB, which was previously generated as a selection-marker-free line, evaluated its propagation, and given that the stored seeds must be renewed periodically. The production of MucoRice-CTB incorporated a closed hydroponic system for cultivating the transgenic plants, to minimize variations in expression and quality during vaccine manufacture. This type of molecular farming factory can be operated year-round, generating three harvests annually, and is cost- and production-effective. Rice was polished to a ratio of 95 % and then powdered to produce the MucoRice-CTB drug substance, and the identity, potency, and safety of the MucoRice-CTB product met pre-established release requirements. The formulation of MucoRice-CTB made by fine-powdering of drug substance and packaged in an aluminum pouch is being evaluated in a physician-initiated phase I study.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Kashima K,Yuki Y,Mejima M,Kurokawa S,Suzuki Y,Minakawa S,Takeyama N,Fukuyama Y,Azegami T,Tanimoto T,Kuroda M,Tamura M,Gomi Y,Kiyono Hdoi
10.1007/s00299-015-1911-9subject
Has Abstractpub_date
2016-03-01 00:00:00pages
667-79issue
3eissn
0721-7714issn
1432-203Xpii
10.1007/s00299-015-1911-9journal_volume
35pub_type
杂志文章abstract：:Plant regeneration from immature embryos of peanut (Arachis hypogaea L.) can be accomplished through somatic embryogenesis. The highest frequency of somatic embryo formation occurred on B5 medium plus 0.5-1.0 mg/l picloram. Shoots and plants developed from the somatic embryos only after extended culture on basal mediu...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00778536
更新日期：1989-04-01 00:00:00
abstract：:An efficient plant regeneration system from leaf-derived callus in 6 genotypes of Avena sativa L. has been established. Regenerable callus was induced in the basal 1-2 mm region of 2 to 5 day old seedlings. Plants were regenerated from the regenerable callus and grown to maturity. The frequency of regenerable callus f...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00238604
更新日期：1995-03-01 00:00:00
abstract：:The nucleotide sequence of the cucumber (Cucumis sativus L. cv. Baekmibaekdadagi) chloroplast genome was completed. The circular double-stranded DNA, consisting of 155,527 bp, contained a pair of inverted repeat regions (IRa and IRb) of 25,187 bp each, which were separated by small and large single copy regions of 86,...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-005-0097-y
更新日期：2006-04-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269152
更新日期：1983-10-01 00:00:00
abstract：KEY MESSAGE:The Arabidopsis SAUR36, renamed RAG1, integrates auxin and gibberellin signals to regulate apical hook maintenance in etiolated seedlings, hypocotyl elongation in the light and fertility. Phytohormone signalling intermediates integrate responses to developmental cues and the variety of environmental inputs ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-013-1406-5
更新日期：2013-06-01 00:00:00
abstract：: Mature ovules of Dianthus (Caryophyllaceae) were histologically observed by clearing and serial sectioning to characterize the cells of the embryo sac. The results show that the mature embryo sac was located deep inside the hemitropous ovule due to thick nucellar tissue at the micropylar region. For the isolation of ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050753
更新日期：2000-04-01 00:00:00
abstract：:Leaf blade tissue of maize inbred lines B73 and Mo17 was analyzed for intraspecific genetic variability in the heat shock response. The maize inbreds were characterized for acquired thermal tolerance and patterns of heat shock protein synthesis. The leakage conductivity assay of membrane stability during stress indica...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00233096
更新日期：1992-10-01 00:00:00
abstract：:The purpose of this study was to develop a new micropropagation system for Tylophora indica, an important medicinal plant in India, using root explants as starting material. Root explants cultured on MS medium supplemented with 6-benzyladenine (BA) or 2-isopentyladenine (2iP) produced organogenic nodular meristemoids ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-003-0753-z
更新日期：2004-05-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-002-0550-0
更新日期：2003-02-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-011-1067-1
更新日期：2011-08-01 00:00:00
abstract：:Rice plants (Oryza sativa L., Chinsurah Boro II var. Indica) were regenerated from protoplasts isolated from microspore derived cell suspensions. A simple procedure for the establishment of such cell suspension cultures from embryogenic microcallus derived from cultured isolated microspores of Indica-type rice is desc...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232295
更新日期：1990-09-01 00:00:00
abstract：:A protocol for protoplast isolation was developed and tested with five Capsicum genotypes representing two cultivated species, C. annuum and C. chinense. Key variables included growth conditions for source plants and the concentration of mannitol used as osmoticum. Protoplasts isolated from each of the genotypes becam...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00234146
更新日期：1994-04-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-020-02634-9
更新日期：2021-02-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-012-1374-1
更新日期：2013-03-01 00:00:00
abstract：:Mimosa tenuiflora (Willd.) Poiret (Leguminosae) was micropropagated throughin vitro culture of axillary buds on Murashige and Skoog (MS) medium. Shoot formation was achieved when the media were supplemented with 0.1 mg.L(-1) IAA + 3 mg.L(-1) KN.In vitro rooting of regenerated shoots was achieved when 0.1 mg.L(-1) KN w...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF01275455
更新日期：1996-11-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232816
更新日期：1993-09-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00231559
更新日期：1990-07-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232378
更新日期：1992-01-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00235072
更新日期：1992-06-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-014-1699-z
更新日期：2015-02-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00236096
更新日期：1993-07-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-011-1096-9
更新日期：2011-10-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-003-0604-y
更新日期：2003-04-01 00:00:00
abstract：:A cDNA clone encoding an ADP-ribosylation factor from potato (Solanum tuberosum L.) was isolated. The nucleotide and deduced amino acid sequences show high homology to known ADP-ribosylation factor sequences from Arabidopsis, yeast, cow and man. In northern blot experiments, all tissues analysed showed expression of t...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00233786
更新日期：1994-12-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00231809
更新日期：1991-03-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-006-0254-y
更新日期：2007-03-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-008-0524-y
更新日期：2008-06-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-006-0129-2
更新日期：2006-07-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章,评审
doi：10.1007/s002990100346
更新日期：2001-07-01 00:00:00
abstract：:Cell suspension cultures of bean (Phaseolus vulgaris) cv. Imuna accumulated isoflavonoid phytoalexins on exposure to elicitor from the phytopathogenic fungus Colletotrichum lindemuthianum (CL). This was preceeded by rapid increases in the activities of phenylalanine ammonia-lyase (PAL) and chalcone synthase (CHS). How...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269060
更新日期：1989-12-01 00:00:00
