Factors influencing transformation frequency of tomato (Lycopersicon esculentum).

Abstract:

:We developed an efficient procedure for transformation and regeneration of L. esculentum cv. Moneymaker from cotyledon explants. The effect of two parameters on the transformation frequency was investigated in detail. The use of feeder layers during cocultivation proved to be critical. In addition, it was found that Agrobacterium strains harbouring a L,L-succinamopine type helper plasmid yielded significantly higher transformation frequencies than those with octopine or nopaline type helper plasmids. The optimized protocol was used to obtain transformation frequencies averaging 9%. Of the plants produced approximately 80% proved to be diploid, of which 67% contained the transgene(s) on a single locus.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

van Roekel JS,Damm B,Melchers LS,Hoekema A

doi

10.1007/BF00232816

subject

Has Abstract

pub_date

1993-09-01 00:00:00

pages

644-7

issue

11

eissn

0721-7714

issn

1432-203X

journal_volume

12

pub_type

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