Production of tumour-inhibitory lignans in callus cultures of Podophyllum hexandrum.

abstract：:Rosmarinic acid (RA) is a natural antioxidant produced by cell suspension cultures of sage (Salvia officinalis L.). The growth and production of RA by these cells can be modified by the type of culture medium. Production can be increased 10-fold to attain 6.4 g.1(-1) under optimal conditions. Investigation of kinetics...

journal_title：Plant cell reports

authors： Hippolyte I,Marin B,Baccou JC,Jonard R

更新日期：1992-04-01 00:00:00

abstract：:Conditions necessary for the isolation and culture of protoplasts from suspension cultures of sugar, fodder and garden beets were investigated. Good yields of protoplasts were obtained by treating cells with a mixture of cellulase, Macerozyme and Driselase enzymes. Nutritional requirements of beet protoplasts were fou...

journal_title：Plant cell reports

authors： Bhat SR,Ford-Lloyd BV,Callow JA

更新日期：1985-12-01 00:00:00

abstract：:An early identification of fusion products was based on the presumed vigorous growth of hybrid calluses after fusion between Solanum brevidens and S. tuberosum leaf protoplasts. The S. brevidens protoplasts were unable to form multicellular colonies under the applied culture conditions. Three size groups of calluses w...

journal_title：Plant cell reports

authors： Polgár Z,Preiszner J,Dudits D,Fehér A

更新日期：1993-05-01 00:00:00

abstract：:Heat shock transcription factors (Hsfs) are the terminal components of the signal transduction chain mediating the activation of genes responsive to both heat stress and a large number of chemical stressors. This paper aims to clone Hsf from lily and characterize its function by analyses of mRNA expression, transactiv...

journal_title：Plant cell reports

authors： Xin H,Zhang H,Chen L,Li X,Lian Q,Yuan X,Hu X,Cao L,He X,Yi M

更新日期：2010-08-01 00:00:00

abstract：:Cultivation of Catharanthus roseus hairy root cultures at different temperatures was found to have an effect on growth rate and indole alkaloid content as well as lipid composition. When lowering the temperature, the roots responded by increasing the degree of unsaturation of cellular lipids, which was mainly due to a...

journal_title：Plant cell reports

authors： Toivonen L,Laakso S,Rosenqvist H

更新日期：1992-07-01 00:00:00

abstract：:Two modes of endocytosis are known to occur in eucaryotic cells: fluid phase and receptor-mediated endocytosis. Fluid-phase endocytosis in plant cells resembles the retrieval of excess plasma membrane material previously incorporated by exocytosis. Pollen tubes need to carry out strong membrane retrieval due to their ...

journal_title：Plant cell reports

authors： Lisboa S,Scherer GE,Quader H

更新日期：2008-01-01 00:00:00

abstract：:The goal of the study was to characterize the optimal developmental stage of zygotic embryo expiants of the hardwood forest tree species yellow-poplar (Liriodendron tulipifera L.) for the initiation of embryogenic cultures, using morphological measurements and polypeptide profiles of the embryos. Developing zygotic em...

journal_title：Plant cell reports

authors： Sotak RJ,Sommer HE,Merkle SA

更新日期：1991-07-01 00:00:00

abstract：:A simple method using molecular hybridization was devised to quantitatively measure chloroplast DNA synthesis in vivo. Total cellular DNA isolated from Nicotiana tabacum suspension cells, labeled with (3)H-thymidine, was hybridized to nitrocellulose membrane-bound cloned chloroplast DNA (ct DNA) fragments. Colorless, ...

journal_title：Plant cell reports

authors： Cannon G,Heinhorst S,Siedlecki J,Weissbach A

更新日期：1985-04-01 00:00:00

abstract：KEY MESSAGE:A complete set of monosomic alien addition lines of Brassica napus with one of the seven chromosomes of Isatis indigotica and the recombinant mitochondria was developed and characterized. Monosomic alien addition lines (MAALs) are valuable for elucidating the genome structure and transferring the useful gen...

journal_title：Plant cell reports

authors： Kang L,Du X,Zhou Y,Zhu B,Ge X,Li Z

更新日期：2014-08-01 00:00:00

abstract：:Four diverse strains of Agrobacterium tumefaciens (C58, Ach5, GV3111, and A281) were capable of inducing tumors at a high frequency on inoculated stems of lentil (Lens culinaris Medik. cultivar Laird) in vivo, and on excised shoot apices in vitro. GV3111 and Ach5 produced the largest and heaviest tumors in vivo, while...

journal_title：Plant cell reports

authors： Warkentin TD,McHughen A

更新日期：1991-12-01 00:00:00

abstract：:The sequences of the 3' untranslated region (UTR) of the manganese superoxide dismutase (MnSOD) genes in wheat (Triticum aestivum) were found to be quite variable with different predicted thermostabilities. The degradation rates of the 3' UTR variants and the coding region were measured following exposure to endogenou...

journal_title：Plant cell reports

authors： Baek KH,Skinner DZ

更新日期：2006-03-01 00:00:00

abstract：:Embryogenic cell suspension cultures of garlic (Allium sativum L.) were initiated in liquid medium from friable embryogenic tissue. The optimal parameters for culture maintenance were: (1) an initial cell density of 1-4% (v/v); (2) medium renewal every 14 days and subculturing every 28 days; (3) a low 2,4-dichlorophen...

journal_title：Plant cell reports

authors： Fereol L,Chovelon V,Causse S,Triaire D,Arnault I,Auger J,Kahane R

更新日期：2005-08-01 00:00:00

abstract：:Transgenic calli of Pharbitis nil which grow in the presence of kanamycin were obtained by introduction of plasmid pBI121, which carries kanamycin resistance and the gene for beta-glucuronidase. The calli were shown to have fragments of vector DNA in their genome and high levels of beta-glucuronidase activity. This is...

journal_title：Plant cell reports

authors： Araki T,Hirano H,Naito S,Komeda Y

更新日期：1989-05-01 00:00:00

abstract：:The hybridity of eleven somatic hybrids between a diploidS. tuberosum and a diploidS. phureja clone could be verified because the parent karyotypes differed in their C-banding patterns. The hybrids were hypotetraploid and some carried structurally rearranged chromosomes and/or minute centric fragments. The nucleolar c...

journal_title：Plant cell reports

authors： Pijnacker LP,Ferwerda MA,Puite KJ,Schaart JG

更新日期：1989-02-01 00:00:00

abstract：:A microscopical study was performed in order to localize chromogenic valepotriates in roots (in vivo) and in callus and root organ cultures (in vitro) of valerian plants. These in vitro cultures (producing valepotriates) contained lipid droplets having the same aspect as those containing essential oil described in the...

journal_title：Plant cell reports

authors： Violon C,Dekegel D,Vercruysse A

更新日期：1983-12-01 00:00:00

abstract：:Accurate and timely detection of transgene copy number in sugarcane is currently hampered by the requirement to use Southern blotting, needing relatively large amounts of genomic DNA and, therefore, the continued growth and maintenance of bulky plants in containment glasshouses. In addition, the sugarcane genome is bo...

journal_title：Plant cell reports

authors： Casu RE,Selivanova A,Perroux JM

更新日期：2012-01-01 00:00:00

abstract：:A novel genic male sterile (GMS) line in Brassica napus L., which was identified in 1999, was found to be controlled by a monogenic dominant gene, which we have designated as MDGMS. The microspores of the MDGMS abort before the degradation of the tapetal cell layer. The F1 fertility from any fertile lines crossed with...

journal_title：Plant cell reports

authors： Wang DJ,Guo AG,Li DR,Tian JH,Huang F,Sun GL

更新日期：2007-05-01 00:00:00

abstract：:The expression of green fluorescent protein (GFP) and its inheritance were studied in transgenic oat ( Avena sativa L.) plants transformed with a synthetic green fluorescent protein gene [sgfp(S65T)] driven by a rice actin promoter. In vitro shoot meristematic cultures (SMCs) induced from shoot apices of germinating m...

journal_title：Plant cell reports

authors： Cho MJ,Choi HW,Okamoto D,Zhang S,Lemaux PG

更新日期：2003-01-01 00:00:00

abstract：:Cell walls and media were obtained from three kinds of carrot cell culture, namely, embryogenic callus (EC), non-embryogenic callus (NC) and somatic embryos (SE), and analyzed for their sugar content and sugar composition by electrophoresis and gas chromatography. EC formed large cell clusters while NC formed small cl...

journal_title：Plant cell reports

authors： Kikuchi A,Satoh S,Nakamura N,Fujii T

更新日期：1995-02-01 00:00:00

abstract：:Embryogenic callus and suspension cultures of carrot (Daucus carota L., cv. Nantaise), growing on/in medium including 1 mg/l 2,4-dichlorophenoxy acetic acid (2,4-D), were transferred to medium with or without this plant growth regulator, to impair or induce, respectively, further development of somatic embryos. The en...

journal_title：Plant cell reports

authors： Jiménez VM,Guevara E,Herrera J,Bangerth F

更新日期：2005-01-01 00:00:00

abstract：:Embryogenic cell suspension cultures were established from Triticum aestivum X Leymus angustus F1 hybrids, using compact nodular calli derived from inflorescence segments. Calli originating from leaf segments did not give rise to stable cell suspensions. Growth measurements of the cell suspensions revealed that they c...

journal_title：Plant cell reports

authors： Tabaeizadeh Z,Campeau N

更新日期：1992-03-01 00:00:00

abstract：:A novel member of the WRKY gene family, designated TcWRKY53, was isolated from a cadmium (Cd)-treated Thlaspi caerulescens cDNA library by differential screening. WRKY proteins specifically bind to W-boxes, which are found in the promoters of many genes involved in defense and response to environmental stress. TcWRKY5...

journal_title：Plant cell reports

authors： Wei W,Zhang Y,Han L,Guan Z,Chai T

更新日期：2008-04-01 00:00:00

abstract：:A restricted part of the undeveloped stem of the garlic clove, called the "stem disc", which is just under the basement of the immature foliage leaves, proved to be a very potent explant for the micropropagation of garlic. Twenty to thirty tissue-cultured shoots consistently were differentiated from a single clove dur...

journal_title：Plant cell reports

authors： Ayabe M,Sumi S

更新日期：1998-07-01 00:00:00

abstract：:Scanning electron microscopy has been used to investigate the morphological changes occurring during the development of alfalfa somatic embryos. Embryos were initiated from callus, transferred to suspension culture and matured on solid agar medium. This developmental pattern was compared to that of zygotic embryos dev...

journal_title：Plant cell reports

authors： Xu N,Bewley JD

更新日期：1992-06-01 00:00:00

abstract：:The effect ofin planta treatments with auxin inhibitors such as 2,3,5 triiodobenzoic acid (TIBA) on regeneration of plantsin vitro is not known. Here, we show the beneficial effect of preconditioning sugarbeet plants in the greenhouse with TIBA (3 mg/1) for efficientin vitro plant regeneration via a callus phase from ...

journal_title：Plant cell reports

authors： Roussy I,Dubois F,Sangwan RS,Sangwan-Norreel BS

更新日期：1996-12-01 00:00:00

abstract：:Growth inhibition towards Rhizopus nigricans, Fusarium oxysporum f. sp. radicis-lycopersici, Verticillium albo-atrum and Pythium ultimum was observed in vitro using a purified chitosanase from an actinomycete, Streptomyces sp, strain N174. The corresponding gene, with its own signal peptide, was inserted into pBI121.7...

journal_title：Plant cell reports

authors： El Quakfaoui S,Potvin C,Brzezinski R,Asselin A

更新日期：1995-12-01 00:00:00

abstract：KEY MESSAGE:We isolated differentially expressed and dark-responsive genes during flower development and opening in petals of morning glory. Flower opening usually depends on petal expansion and is regulated by both genetic and environmental factors. Flower opening in morning glory (Ipomoea nil) is controlled by the da...

journal_title：Plant cell reports

authors： Shinozaki Y,Tanaka R,Ono H,Ogiwara I,Kanekatsu M,van Doorn WG,Yamada T

更新日期：2014-07-01 00:00:00

abstract：:A method to obtain plants from embryogenic callus of Brassica nigra and protoplasts of hypocotyl expiants is described. Callus was initiated on Murashige and Skoog medium containing kinetin (kn) and 2,4-dichlorophenoxy acetic acid (2,4-D). Lowering of auxin induced embryo formation. Supplementation with gibberellic ac...

journal_title：Plant cell reports

authors： Gupta V,Agnihotri A,Jagannathan V

更新日期：1990-12-01 00:00:00

abstract：KEY MESSAGE:Overexpression of Withania somnifera SGT gene (WssgtL3.1) in transgenic Arabidopsis improves various agronomic and physiological traits and alters conjugated sterol levels to mitigate the effect of salt stress. Sterols are essential constituents of cell membranes that are involved in several biological func...

journal_title：Plant cell reports

authors： Mishra MK,Tiwari S,Misra P

更新日期：2021-02-01 00:00:00

abstract：KEY MESSAGE:We report global gene expression patterns of poplar in response to chilling stress. A total of 1,085 significantly differentially expressed genes, involved in photosynthesis, signal transduction, and regulation of transcription, were identified. To understand the gene network underlying the response to chil...

journal_title：Plant cell reports

authors： Song Y,Chen Q,Ci D,Zhang D

更新日期：2013-09-01 00:00:00