Abstract:
:A novel member of the WRKY gene family, designated TcWRKY53, was isolated from a cadmium (Cd)-treated Thlaspi caerulescens cDNA library by differential screening. WRKY proteins specifically bind to W-boxes, which are found in the promoters of many genes involved in defense and response to environmental stress. TcWRKY53 contains a 975-bp open reading frame encoding a putative protein of 324 amino acids. Homology searches showed that TcWRKY53 resembles similar WRKY domain-containing proteins from rice, parsley and tobacco, especially AtWRKY53 from Arabidopsis thaliana. Semi-quantitative RT-PCR showed that the expression of TcWRKY53 was strongly induced by various environmental stresses, including an excess of NaCl, drought, cold and the signal molecule salicylic acid (SA). The expression of TcWRKY53 in response to NaCl, drought and cold suggested a possible role of TcWRKY53 in abiotic stress response. However, physiological tests indicated that the expression of TcWRKY53 in tobaccos decreases tolerance to sorbitol during seedling root development. This was consistent with PEG6000 treatment of tobacco seedlings, and together these results indicate a negative modulation of TcWRKY53 in response to osmotic stress. Furthermore, two ethylene responsive factor (ERF) family genes, NtERF5 and NtEREBP-1, were negatively induced in TcWRKY53-overexpressing transgenic plants. In contrast, a LEA family gene, NtLEA5, showed no change, suggesting that TcWRKY53 might regulate the plant osmotic stress response by interacting with an ERF-type transcription factor rather than by regulating function genes directly.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Wei W,Zhang Y,Han L,Guan Z,Chai Tdoi
10.1007/s00299-007-0499-0subject
Has Abstractpub_date
2008-04-01 00:00:00pages
795-803issue
4eissn
0721-7714issn
1432-203Xjournal_volume
27pub_type
杂志文章abstract::Genistein, 4',5,7-trihydroxyisoflavone, is an isoflavonoid compound predominantly restricted to legumes and known to possess phyto-oestrogenic and antioxidative activities. The key enzyme that redirects phenylpropanoid pathway intermediates from flavonoids to isoflavonoids is the isoflavone synthase (IFS). Brassica na...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1052-8
更新日期:2011-08-01 00:00:00
abstract::Suspension cultured cells of the moss, Barbula unguiculata, grow actively in both light and dark culture. Light-grown cells contain chlorophyll and exhibit an undifferentiated callus form. When cells are transferred to a dark condition, they develop into protonemata. Protonemata formation in the dark can be inhibited ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00272039
更新日期:1989-03-01 00:00:00
abstract::Responses induced by Cd exposure were assessed in tobacco seedlings (Nicotiana tabacum L.) using macro and molecular indices. The 100 microM of Cd exposure reduced the total dry weight and chlorophyll index of the seedlings as much as the genuine Fe-deficiency. Concentration of Fe in the shoots decreased, whereas that...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-005-0092-3
更新日期:2006-04-01 00:00:00
abstract::Leaf explants ofKalanchoe laciniata were cocultivated for different days (2, 4, 6 and 8 days) with disarmedAgrobacterium tumefaciens strains A208SE, GV3111SE and EHA101 carring a binary vector pROA93. The vector contains a cauliflower mosaic virus 35S promotor which drives the coding sequence of neomycin phosphotransf...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00716668
更新日期:1989-06-01 00:00:00
abstract::RNA interference (RNAi) is a sequence-specific, posttranscriptional gene silencing (PTGS) process in plants that is mediated by dsRNA homologous to the silenced gene(s). In this study, we report an efficient method to produce dsRNA using a bacterial expression system. Two fragments of the Sugarcane Mosaic Virus (SCMV)...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-010-0911-z
更新日期:2010-11-01 00:00:00
abstract::Factors involved in promoting caulogenesis from hypocotyl explants of Phyllanthus fraternus were studied. Hypocotyl explants were cultured on B5 medium supplemented with 2,4-D or NAA in the presence and absence of BAP (at concentrations 0, 10(-7), 10(-6) and 10(-5)M). Adventitious shoots differentiated from callus dev...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232932
更新日期:1994-08-01 00:00:00
abstract:KEY MESSAGE:Thirty genes involved in GA and ABA metabolism and signalling were identified, and the expression profiles indicated that they play crucial roles in the bud activity-dormancy transition in tea plants. Gibberellin (GA) and abscisic acid (ABA) are fundamental phytohormones that extensively regulate plant grow...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-017-2238-5
更新日期:2018-03-01 00:00:00
abstract:: Embryogenic nucellar callus cultures of different Citrus species and cultivars growing in hormone-free medium were transferred to medium containing either sucrose or glycerol as the only carbohydrate source. Glycerol has been reported to induce further development of Citrus somatic embryos, while in the presence of s...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990000280
更新日期:2001-01-01 00:00:00
abstract::Plants have their own mechanisms for overcoming various stresses. In cold regions, plants are subject to stress and must enter an inherent dormancy, through several complex mechanisms, if they are to continue to exist. In winter, regulation of tonoplast and plasma membrane aquaporin genes differed in the bud cushions ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-009-0770-7
更新日期:2009-11-01 00:00:00
abstract::The effect ofin planta treatments with auxin inhibitors such as 2,3,5 triiodobenzoic acid (TIBA) on regeneration of plantsin vitro is not known. Here, we show the beneficial effect of preconditioning sugarbeet plants in the greenhouse with TIBA (3 mg/1) for efficientin vitro plant regeneration via a callus phase from ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF01890855
更新日期:1996-12-01 00:00:00
abstract::Morphologically normal green plants have reproducibly been regenerated from protoplasts of an Australian wheat (Triticum aestivum cv. Hartog). The protoplasts were isolated from fine embryogenic suspension cultures which were initiated from embryogenic callus. Protoplasts were incubated in a modified liquid MS medium ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00231832
更新日期:1992-02-01 00:00:00
abstract::Transgenic papaya (Carica papaya L.) plants were regenerated from embryogenic cultures that were cocultivated with a disarmed C58 strain of Agrobacterium tumefaciens containing one of the following binary cosmid vectors: pGA482GG or pGA482GG/cpPRV-4. The T-DNA region of both binary vectors includes the chimeric genes ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00237128
更新日期:1993-03-01 00:00:00
abstract::Intertribal somatic hybrids between Brassica napus (2n = 38, AACC) and a dye and medicinal plant Isatis indigotica (2n = 14, II) were obtained by fusions of mesophyll protoplasts. From a total of 237 calli, only one symmetric hybrid (S2) and five asymmetric hybrids (As1, As4, As6, As7 and As12) were established in the...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-009-0712-4
更新日期:2009-07-01 00:00:00
abstract::Embryogenic cell suspension cultures of garlic (Allium sativum L.) were initiated in liquid medium from friable embryogenic tissue. The optimal parameters for culture maintenance were: (1) an initial cell density of 1-4% (v/v); (2) medium renewal every 14 days and subculturing every 28 days; (3) a low 2,4-dichlorophen...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-005-0937-9
更新日期:2005-08-01 00:00:00
abstract::A NaCl-tolerant callus line of Cicer arietinum has been isolated, as a spontaneous variant, on agar-solidified MS-medium supplemented with 100 mM NaCl. The growth of this line, in the presence of 100 mM NaCl, was comparable to that of the NaCl-sensitive callus line growing in the absence of NaCl. Regarding relative to...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00270968
更新日期:1984-04-01 00:00:00
abstract::The article 'Instability of endosperm development in amphiploids and their parental species in the genus Avena L.', written by Paulina Tomaszewska and Romuald Kosina, was originally published electronically on the publisher's internet portal on 22 May with an incorrect copyright line. As the article was due to be publ...
journal_title:Plant cell reports
pub_type: 杂志文章,已发布勘误
doi:10.1007/s00299-018-2337-y
更新日期:2019-01-01 00:00:00
abstract::A double-gene construct with one chitinase and one beta-1,3-glucanase gene from barley, both driven by enhanced 35S promoters, was transformed into oilseed rape. From six primary transformants expressing both transgenes 10 doubled haploid lines were produced and studied for five generations. The number of inserted cop...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-006-0153-2
更新日期:2006-09-01 00:00:00
abstract::Agrobacterium-mediated transformation frequency is very low with cells from some species such as soybeans. Studies were conducted to investigate the Agrobacterium-mediated transformation frequency in near-isogenic lipoxygenase mutant lines of soybeans, since the nigh level of lipoxygenase activity in soybean embryos m...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00231809
更新日期:1991-03-01 00:00:00
abstract:: Identifying transformed plant lines carrying the antibiotic resistance marker gene, neomycin phosphotransferase II, requires a more definitive test than the ability of the plant to grow on kanamycin. Although a number of alternative assays have been described, most are cumbersome, time consuming and/or require the us...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050014
更新日期:2000-01-01 00:00:00
abstract::Grapefruit (Citrus paradisi) tissue cultures were examined for qualitative and quantitative changes in flavanone-neohesperidoside content during somatic embryogenesis. Embryos cultured in vitro contain naringin and a rhamnosyl-transferase activity which is capable of rhamnosylating position 2 on the flavanone glucosid...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00270076
更新日期:1989-10-01 00:00:00
abstract::A protocol for protoplast isolation was developed and tested with five Capsicum genotypes representing two cultivated species, C. annuum and C. chinense. Key variables included growth conditions for source plants and the concentration of mannitol used as osmoticum. Protoplasts isolated from each of the genotypes becam...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00234146
更新日期:1994-04-01 00:00:00
abstract::Capsella bursa-pastoris (L.) Medic (2n = 4x = 32) is a natural double-low (erucic acid < 1%, glucosinolates < 30 micromol/g) germplasm and shows high degree of resistance to Sclerotinia sclerotiorum. Hybridizations were carried out between two Brassica species viz. B. rapa (2n = 20) and B. napus (2n = 38) as female an...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-007-0392-x
更新日期:2007-10-01 00:00:00
abstract::Plants have developed diverse molecular mechanisms to resist viruses. RNA silencing plays a dominant role in antiviral defense. Recent studies have correlated plant antiviral silencing to epigenetic modification in genomic DNA and protein by remodeling the expression levels of coding genes. The plant host methylation ...
journal_title:Plant cell reports
pub_type: 杂志文章,评审
doi:10.1007/s00299-019-02414-0
更新日期:2019-09-01 00:00:00
abstract::A simple and reliable method was developed for isolating pollen nuclei from Brassica napus and Triticum aestivum for DNA analysis using flow cytometry. The nuclei were released from pollen by ultrasonic treatment. The isolated nuclei following filtration through nylon mesh and a purification procedure were suitable fo...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-004-0830-y
更新日期:2004-10-01 00:00:00
abstract:KEY MESSAGE:In GhCFE5 homoeologs, GhCFE5D interacted with more actin homologs and stronger interaction activity than GhCFE5A. GhCFE5D - but not GhCFE5A -overexpression severely disrupted actin cytoskeleton organization and significantly suppressed cell elongation. Homoeologous genes are common in polyploid plants; howe...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-015-1928-0
更新日期:2016-04-01 00:00:00
abstract::In a study of the 5'-flanking sequence of the Zea mays L. (maize) Glb1 gene in vitro, serial promoter deletions were generated and linked with the β-glucuronidase (GUS) reporter gene. The promoter deletion-GUS fusions were introduced into the maize P3377 cell line by particle bombardment. GUS assays indicated that tre...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050459
更新日期:1998-05-01 00:00:00
abstract::Morphologically normal and fertile transgenic plants of mungbean with two transgenes, bar and alpha-amylase inhibitor, have been developed for the first time. Cotyledonary node explants were transformed by cocultivation with Agrobacterium tumefaciens strain EHA105 harboring a binary vector pKSB that carried bialaphos ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-006-0224-4
更新日期:2007-02-01 00:00:00
abstract::An efficient plant regeneration system from leaf-derived callus in 6 genotypes of Avena sativa L. has been established. Regenerable callus was induced in the basal 1-2 mm region of 2 to 5 day old seedlings. Plants were regenerated from the regenerable callus and grown to maturity. The frequency of regenerable callus f...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00238604
更新日期:1995-03-01 00:00:00
abstract:KEY MESSAGE:A complete set of monosomic alien addition lines of Brassica napus with one of the seven chromosomes of Isatis indigotica and the recombinant mitochondria was developed and characterized. Monosomic alien addition lines (MAALs) are valuable for elucidating the genome structure and transferring the useful gen...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-014-1621-8
更新日期:2014-08-01 00:00:00
abstract::A protocol was developed for biolistic transformation of hybrid bermudagrass cv. TifEagle using the bar gene. TifEagle is an ultradwarf used exclusively on golf greens. Herbicide resistance should serve as a useful management tool, especially if methyl-bromide is unavailable for fumigation prior to plant establishment...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0736-0
更新日期:2004-03-01 00:00:00