Abstract：
:In a study of the 5'-flanking sequence of the Zea mays L. (maize) Glb1 gene in vitro, serial promoter deletions were generated and linked with the β-glucuronidase (GUS) reporter gene. The promoter deletion-GUS fusions were introduced into the maize P3377 cell line by particle bombardment. GUS assays indicated that treatment of the maize cultured cells with abscisic acid (ABA) was required for Glb1-driven GUS transient expression, and that the -272-bp sequence of the Glb1 promoter was sufficient for ABA-regulated expression of GUS. The longest undeleted sequence used, -1391 GUS, showed relatively low expression which could be indicative of an upstream silencer element in the Glb1 promoter between -1391 and -805. Further studies show that the Glb1-driven GUS activity of bombarded maize P3377 cells increases with increasing ABA concentration (up to 100-300 μM). Site-directed mutagenesis of a putative ABA response element, Em1a, abolished GUS expression in P3377 cells. This observation indicated that the Em1a sequence in the Glb1 5' regulatory region is responsible for the positive ABA regulation of gene expression.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Liu S,Kriz A,Duncan D,Widholm Jdoi
10.1007/s002990050459subject
Has Abstractpub_date
1998-05-01 00:00:00pages
650-655issue
8eissn
0721-7714issn
1432-203Xpii
10.1007/s002990050459journal_volume
17pub_type
杂志文章abstract：:High yields of protoplasts were obtained from leaves of aseptically grown plants and calli originated from different explants, in several cultivars of Cajanus cajan L. The protoplasts divided to form cell clusters in modified KM 8p medium and developed to protocolonies after dilution with liquid Caboche's medium withi...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232691
更新日期：1992-08-01 00:00:00
abstract：:The regeneration of somatic seedlings from selected 100-year-old cork oak trees is reported. The induction of somatic embryogenesis from leaves of epicormic shoots was significantly affected by genotype, harvesting time and their interaction. Leaves from all five selected trees produced somatic embryos when the segmen...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-003-0604-y
更新日期：2003-04-01 00:00:00
abstract：:Albinism remains a major problem in cereal improvement programs that rely on doubled haploid (DH) technology, and the factors controlling the phenomenon are not well understood. Here we report on the positive influence of copper on the production of DH plants obtained through microspore embryogenesis (ME) in recalcitr...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-009-0733-z
更新日期：2009-09-01 00:00:00
abstract：:Phytohormones are essential signal compounds in the regulation of stress-related and defense-related genes. However, there is no clear evidence for any effect of these signal molecules and biotic elicitors on the regulation of the SALT gene in suspension-cultured rice cells. We characterized the expression of a SALT g...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-004-0836-5
更新日期：2004-10-01 00:00:00
abstract：: Identifying transformed plant lines carrying the antibiotic resistance marker gene, neomycin phosphotransferase II, requires a more definitive test than the ability of the plant to grow on kanamycin. Although a number of alternative assays have been described, most are cumbersome, time consuming and/or require the us...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050014
更新日期：2000-01-01 00:00:00
abstract：KEY MESSAGE:Cadmium sensitivity in sultr1;1 - sultr1;2 double mutant with limiting sulfate supply is attributed to the decreased glutathione content that affected oxidative defense but not phytochelatins' synthesis. In plants, glutathione (GSH) homeostasis plays pivotal role in cadmium (Cd) detoxification. GSH is synth...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-015-1892-8
更新日期：2016-02-01 00:00:00
abstract：:The effects of serial combinations of either indole-3-acetic acid, indole-3-butyric acid or α-naphthaleneacetic acid (0.5-10.0 mg/l) with either kinetin, 6-benzyl-amino-purine, zeatin or 6-methylaminopurine (0.5-5.0 mg/l) have been investigated to assess the morphogenetic potential of foliar explants of Duboisia myopo...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269711
更新日期：1986-02-01 00:00:00
abstract：UNLABELLED:Musa acuminata ssp. burmannica, one of the wild progenitors contributing 'A genome' to the present-day dessert bananas, has a long evolutionary history intervened by human activities. In this study, ISSR markers were used to analyze the pattern of genetic variation and differentiation in 32 individuals along...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-012-1273-5
更新日期：2012-09-01 00:00:00
abstract：:A test system for root regeneration was developed that consists of stem slices of apple shoots (ca. 0.5 mm thick; fresh weight ca. 1 mg). Roots regenerated synchronously without intervening callus formation and without interference with compounds originating from other parts of the plant. Supply of indolebutyric acid ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00237054
更新日期：1993-02-01 00:00:00
abstract：:Emission and plant uptake of atmospheric nitrogen oxides (NO + NO(2)) significantly influence regional climate change by regulating the oxidative chemistry of the lower atmosphere, species composition and the recycling of carbon and nutrients, etc. Plant uptake of nitrogen dioxide (NO(2)) is concentration-dependent an...
journal_title：Plant cell reports
pub_type： 杂志文章,评审
doi：10.1007/s00299-010-0898-5
更新日期：2010-10-01 00:00:00
abstract：UNLABELLED:Calmodulin-binding proteins (CBPs) have been known to be involved in both biotic and abiotic stress responses. Recently, two closely related CBPs, Arabidopsis SAR Deficient 1 and CBP60g, were found to belong to a new family of transcription factors that regulate salicylic acid (SA) biosynthesis triggered by ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-012-1247-7
更新日期：2012-07-01 00:00:00
abstract：:A protocol for whole plant regeneration of Cicer arietinum L. cv. C-235 via organogenesis from callus has been developed. Callus initiation was best when immature leaflets were cultured on MS medium containing 5 or 25 μM 2,4-D or NAA in combination with 10 μM BA, or 25 μM 2,4-D alone. The callus grew most vigorously o...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232946
更新日期：1994-06-01 00:00:00
abstract：:The full-length cDNAs of eight S ribonucleases (S-RNases) were cloned from stylar RNA of European pear cultivars that could not be characterized by the cleaved amplified polymorphic sequences (CAPS) marker system for genotyping European pear cultivars harboring nine S alleles Sa, Sb, Sd, Se, Sh, Sk, Sl, Sq, and Sr. Co...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-006-0254-y
更新日期：2007-03-01 00:00:00
abstract：:Phenylalanine ammonia-lyase [PAL, EC 4.3.1.24 (formerly EC 4.3.1.5)], functions in the plant phenylpropanoid biosynthetic pathway to deaminate the amino acid L-phenylalanine forming trans-cinnamic acid and ammonia. The human inherited metabolic disorder phenylketonuria (PKU) is characterized by an inability of individ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-008-0650-6
更新日期：2009-03-01 00:00:00
abstract：:In this paper we describe a simple method to improve the plating efficiency in plant cell cultures.Two-stage plating is used; in the first stage the cells are inoculated at high density in 0.2% agarized culture medium for ten days to facilitate growth; under this condition, each cell produces a single micro-colony tra...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00571305
更新日期：1985-05-01 00:00:00
abstract：:KEY MESSAGE : Antioxidant activity of seven leafy vegetables and four beans against five reactive oxygen species and reactive nitrogen species was clearly characterized with a protocol using myoglobin as a reporter probe. ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-012-1368-z
更新日期：2013-03-01 00:00:00
abstract：KEY MESSAGE:Phosphate starvation increased the production of phenolic acids by inducing the key enzyme genes in a positive feedback pathway in Saliva miltiorrhiza hairy roots. SPX may be involved in this process. Salvia miltiorrhiza is a wildly popular traditional Chinese medicine used for the treatment of coronary hea...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-016-2007-x
更新日期：2016-09-01 00:00:00
abstract：:The nuclear DNA content of sweet pepper (Capsicum annuum L. var. annuum, 2n = 24) has been measured by flow and image cytometries but the DNA content of each chromosome of this species has not yet been regarded. DNA content of individual chromosomes has been quantified by the flow karyotyping technique, which requires...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-008-0539-4
更新日期：2008-07-01 00:00:00
abstract：:Morphologically normal and fertile transgenic plants of mungbean with two transgenes, bar and alpha-amylase inhibitor, have been developed for the first time. Cotyledonary node explants were transformed by cocultivation with Agrobacterium tumefaciens strain EHA105 harboring a binary vector pKSB that carried bialaphos ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-006-0224-4
更新日期：2007-02-01 00:00:00
abstract：KEY MESSAGE:The oil palm EgPAL1 gene promoter and its regulatory region were functional as a promoter in the heterologous system of Arabidopsis according to the cis-acting elements present in that region. The promoter was developmentally regulated, vascular tissue specific and responsive to water stress agents. Phenyla...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-017-2228-7
更新日期：2018-02-01 00:00:00
abstract：:Somatic embryos and plantlets have been obtained in quite a direct way from mesophyll protoplasts isolated from androgenetic plants of two cultivars ("Loras" and "Tower") of Brassica napus. The procedure consists of four steps. Proembryos were induced in a medium supplemented with a cytokinin and an auxin at comparati...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00270237
更新日期：1982-10-01 00:00:00
abstract：:Cell suspension cultures of Nicotiana were initiated using conditions designed to selectively favor stable chromosome number. These conditions included use of leaf explants to initiate cultures, growth of cells in culture medium containing 2,4-D, and transfer of cells with short subculture intervals. Four cell lines d...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00272364
更新日期：1982-04-01 00:00:00
abstract：KEY MESSAGE:Transcriptomes of two switchgrass genotypes representing the upland and lowland ecotypes will be key tools in switchgrass genome annotation and biotic and abiotic stress functional genomics. Switchgrass (Panicum virgatum L.) is an important bioenergy feedstock for cellulosic ethanol production. We report ge...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-016-2065-0
更新日期：2017-01-01 00:00:00
abstract：:Diuraphis noxia (Russian wheat aphid, RWA) is a major pest on wheat in South Africa and most other wheat growing countries. Being a probing-sucking insect, RWAs insert their stylets into the phloem sieve elements and feed on the phloem sap. This feeding causes necrotic lesions in resistant varieties, or decoloration o...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-005-0001-9
更新日期：2006-02-01 00:00:00
abstract：: Alginate-coated meristems from in vitro-grown axillary buds of mint (Mentha spicata L.) were successfully cryopreserved by vitrification. Excised meristems from nodal segments cold hardened at 4 °C for 3 weeks were encapsulated and osmoprotected by a mixture of 2 M glycerol plus 0.4 M sucrose. These meristems were d...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050725
更新日期：1999-12-01 00:00:00
abstract：: Embryogenic nucellar callus cultures of different Citrus species and cultivars growing in hormone-free medium were transferred to medium containing either sucrose or glycerol as the only carbohydrate source. Glycerol has been reported to induce further development of Citrus somatic embryos, while in the presence of s...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990000280
更新日期：2001-01-01 00:00:00
abstract：:Photosynthetically active callus and cell suspension cultures were established from uninfected Lycopersicon peruvianum plants and from uninfected and potato spindle tuber viroid (PSTVd) infected plants of Lycopersicon esculentum cv. Rutgers. Viroid infection was maintained in photoheterotrophic culture on media contai...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232806
更新日期：1993-09-01 00:00:00
abstract：:The purpose of this study was to develop a new micropropagation system for Tylophora indica, an important medicinal plant in India, using root explants as starting material. Root explants cultured on MS medium supplemented with 6-benzyladenine (BA) or 2-isopentyladenine (2iP) produced organogenic nodular meristemoids ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-003-0753-z
更新日期：2004-05-01 00:00:00
abstract：: St. John's wort (Hypericum perforatum. cv 'Anthos') is a medicinal plant with evidence of efficacy as an anti-depressant. The present report describes the development of an in vitro regeneration system that utilizes thidiazuron [N-phenyl-N'-(1,2,3-thidiazol-yl)urea] for the induction of de novo shoots on etiolated hy...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050776
更新日期：2000-05-01 00:00:00
abstract：:High yields of viable protoplasts were obtained by enzymatic treatment from cotyledonary leaves of various greenhouse grown Phaseolus vulgaris L. cultivars. The protoplasts divided and formed cell clusters in a liquid medium. Early transfer before 10 days in the same medium was necessary for the development of cell co...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269250
更新日期：1986-04-01 00:00:00
