Abstract:
:Albinism remains a major problem in cereal improvement programs that rely on doubled haploid (DH) technology, and the factors controlling the phenomenon are not well understood. Here we report on the positive influence of copper on the production of DH plants obtained through microspore embryogenesis (ME) in recalcitrant cultivars of barley (Hordeum vulgare L.). The presence of copper sulphate in the anther pre-treatment medium improved green DH plant regeneration from cultivars known to produce exclusively albino plants using classical procedures. In plastids, the effect of copper was characterized by a decrease in starch and a parallel increase in internal membranes. The addition of copper sulphate in the ME pre-treatment medium should enable breeders to exploit the genetic diversity of recalcitrant cultivars through DH technology. We examined programmed cell death (PCD) during microspore development to determine whether PCD may interfere with the induction of ME and/or the occurrence of albinism. By examining the fate of nuclei in various anther cell layers, we demonstrated that the kinetics of PCD in anthers differed between the barley cultivars Igri and Cork that show a low and a high rate of albinism, respectively. However, no direct correlation between PCD in the anther cell layers and the rate of albinism was observed and copper had no influence on the PCD kinetic in these cultivars. It was concluded that albinism following ME was not due to PCD in anthers, but rather to another unknown phenomenon that appears to specifically affect plastids during microspore/pollen development.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Jacquard C,Nolin F,Hécart C,Grauda D,Rashal I,Dhondt-Cordelier S,Sangwan RS,Devaux P,Mazeyrat-Gourbeyre F,Clément Cdoi
10.1007/s00299-009-0733-zsubject
Has Abstractpub_date
2009-09-01 00:00:00pages
1329-39issue
9eissn
0721-7714issn
1432-203Xjournal_volume
28pub_type
杂志文章abstract::Shoot buds of ginger were successfully encapsulated in 4% sodium alginate gel. Encapsulated buds were germinated in vitro to form roots and shoots. In vitro germination (emergence of sprouts) of encapsulated buds ranged from 16.7% to 81.8% on different media after 5 weeks of incubation. Normal plantlets with an averag...
journal_title:Plant cell reports
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journal_title:Plant cell reports
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journal_title:Plant cell reports
pub_type: 杂志文章
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