Characterization of a chalcone synthase (CHS) flower-specific promoter from Lilium orential 'Sorbonne'.

Abstract:

:The first enzyme in the flavonoid pathway, chalcone synthase, is encoded by a gene (CHS) whose expression is normally under developmental control. In our previous studies, an 896-bp promoter region of a flower-specific CHS gene was isolated from Lilium orential 'Sorbonne', and designated as PLoCHS. Here, the PLoCHS promoter was fused to the β-glucuronidase (GUS) gene to characterize its spatial and temporal expression in Petunia hybrida 'Dreams Midnight' using an Agrobacterium-mediated leaf disc transformation method. Our results demonstrated that GUS expression was present in flowers, but reduced or absent in the other tissues (leaf and stem) examined. In petals, GUS activity reached its peak at flower developmental stage 4, and decreased at later stages. Deletion analysis indicated that even a 307-bp fragment of the PLoCHS promoter could still direct flower-specific expression. Further deletion of the region from -261 to -72 bp resulted in weak expression in different organs, including flowers, leaves and stems. This evidence combined with prediction of cis-acting elements in the PLoCHS promoter suggests that the TACPyAT box located in this promoter plays a key role in the regulation of organ-specific expression.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Liu Y,Lou Q,Xu W,Xin Y,Bassett C,Wang Y

doi

10.1007/s00299-011-1124-9

subject

Has Abstract

pub_date

2011-12-01 00:00:00

pages

2187-94

issue

12

eissn

0721-7714

issn

1432-203X

journal_volume

30

pub_type

杂志文章
  • Development of a complete set of monosomic alien addition lines between Brassica napus and Isatis indigotica (Chinese woad).

    abstract:KEY MESSAGE:A complete set of monosomic alien addition lines of Brassica napus with one of the seven chromosomes of Isatis indigotica and the recombinant mitochondria was developed and characterized. Monosomic alien addition lines (MAALs) are valuable for elucidating the genome structure and transferring the useful gen...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-014-1621-8

    authors: Kang L,Du X,Zhou Y,Zhu B,Ge X,Li Z

    更新日期:2014-08-01 00:00:00

  • Selection of large quantities of embryogenic calli from indica rice seeds for production of fertile transgenic plants using the biolistic method.

    abstract::The microprojectile bombardment of immature embryos has proven to be effective in transforming many indica rice varieties. One of the drawbacks of using immature embryos is the requirement of a large number of high quality immature embryos, which itself is a tedious and laborious process. To circumvent these problems,...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00232364

    authors: Sivamani E,Shen P,Opalka N,Beachy RN,Fauquet CM

    更新日期:1996-01-01 00:00:00

  • Effects of cadmium stress on the antioxidant system and chlorophyll fluorescence characteristics of two Taxodium clones.

    abstract:KEY MESSAGE:The T.118 and T.406 seedlings showed strong adaptability under Cd concentrations ≤ 50 µM. The mechanisms of photoprotection in T.118 and T.406 differed in high-Cd concentrations. To explore the physiological response characteristics of Taxodium hybrids to cadmium (Cd) stress and provide basis for screening ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-018-2327-0

    authors: Ma YL,Wang HF,Wang P,Yu CG,Luo SQ,Zhang YF,Xie YF

    更新日期:2018-11-01 00:00:00

  • Effects of drought-re-watering-drought on the photosynthesis physiology and secondary metabolite production of Bupleurum chinense DC.

    abstract:KEY MESSAGE:Drastic changes in soil water content can activate the short-term high expression of key enzyme-encoding genes involved in secondary metabolite synthesis thereby increasing the content of secondary metabolites. Bupleurum chinense DC. is a traditional medicinal herb that is famous for its abundant saikosapon...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-019-02436-8

    authors: Yang L,Zhao Y,Zhang Q,Cheng L,Han M,Ren Y,Yang L

    更新日期:2019-09-01 00:00:00

  • Agrobacterium tumefaciens-mediated transformation of an Oncidium orchid.

    abstract::The present protocol was aimed at establishing a routine transformation procedure via Agrobacterium tumefaciens for an important Oncidium orchid cultivar. An expression vector containing hptII and gusA genes driven by the cauliflower mosaic virus (CaMV) 35S promoter was successfully introduced into the Oncidium orchid...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-003-0614-9

    authors: Liau CH,You SJ,Prasad V,Hsiao HH,Lu JC,Yang NS,Chan MT

    更新日期:2003-06-01 00:00:00

  • A model wheat cultivar for transformation to improve resistance to Fusarium Head Blight.

    abstract::Fusarium head blight (FHB), caused primarily by Fusarium graminearum, is a major disease problem in wheat (Triticum aestivum). Genetic engineering holds significant potential to enhance FHB resistance in wheat. Due to the requirement of screening for FHB resistance on flowers at anthesis, the number of screens carried...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-005-0059-4

    authors: Mackintosh CA,Garvin DF,Radmer LE,Heinen SJ,Muehlbauer GJ

    更新日期:2006-04-01 00:00:00

  • Phosphate starvation promoted the accumulation of phenolic acids by inducing the key enzyme genes in Salvia miltiorrhiza hairy roots.

    abstract:KEY MESSAGE:Phosphate starvation increased the production of phenolic acids by inducing the key enzyme genes in a positive feedback pathway in Saliva miltiorrhiza hairy roots. SPX may be involved in this process. Salvia miltiorrhiza is a wildly popular traditional Chinese medicine used for the treatment of coronary hea...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-016-2007-x

    authors: Liu L,Yang D,Liang T,Zhang H,He Z,Liang Z

    更新日期:2016-09-01 00:00:00

  • Isolation of embryo sacs from Dianthus ovules by enzymatic treatments and microdissection.

    abstract:: Mature ovules of Dianthus (Caryophyllaceae) were histologically observed by clearing and serial sectioning to characterize the cells of the embryo sac. The results show that the mature embryo sac was located deep inside the hemitropous ovule due to thick nucellar tissue at the micropylar region. For the isolation of ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s002990050753

    authors: Hoshino Y,Nishino E,Mii M

    更新日期:2000-04-01 00:00:00

  • Isolation and functional characterization of a R2R3-MYB regulator of the anthocyanin biosynthetic pathway from Epimedium sagittatum.

    abstract:KEY MESSAGE:A R2R3-MYB transcription factor EsAN2 was isolated from Epimedium sagittatum and functionally characterized to regulate the anthocyanin biosynthetic pathway. Epimedium plants are used widely both as traditional Chinese medicinal herbs and ornamental perennials. Anthocyanins, acting as major contributors to ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-015-1929-z

    authors: Huang W,Khaldun AB,Lv H,Du L,Zhang C,Wang Y

    更新日期:2016-04-01 00:00:00

  • Thermoinduction of genes encoding the enzymes of gibberellin biosynthesis and a putative negative regulator of gibberellin signal transduction in Eustoma grandiflorum.

    abstract::Eustoma grandiflorum Shinn requires vernalization for the induction of stem elongation and flowering. To investigate the role of gibberellins (GAs) in vernalization, the expression levels of genes encoding enzymes of GA biosynthesis, copalyl diphosphate synthetase, GA 20-oxidase and GA 3 beta-hydroxylase, were examine...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-003-0672-z

    authors: Mino M,Oka M,Tasaka Y,Iwabuchi M

    更新日期:2003-09-01 00:00:00

  • Analysis of somaclonal variation in transgenic and regenerated plants of Arabidopsis thaliana using methylation related metAFLP and TMD markers.

    abstract:KEY MESSAGE:We provide evidence that nucleotide sequence and methylation status changes occur in the Arabidopsis genome during in vitro tissue culture at a frequency high enough to represent an important source of variation. Somaclonal variation is a general consequence of the tissue culture process that has to be anal...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-017-2217-x

    authors: Coronel CJ,González AI,Ruiz ML,Polanco C

    更新日期:2018-01-01 00:00:00

  • Genetic relatedness of Portuguese almond cultivars assessed by RAPD and ISSR markers.

    abstract::Randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers were used to analyse the genetic diversity of Portuguese Prunus dulcis cultivars and their relationship to important foreign cultivars. Of the primers tested, 6 (out of 60) RAPD and 5 (out of 18) ISSR primers were selected for th...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-003-0659-9

    authors: Martins M,Tenreiro R,Oliveira MM

    更新日期:2003-08-01 00:00:00

  • An efficient method for flow cytometric analysis of pollen and detection of 2n nuclei in Brassica napus pollen.

    abstract::A simple and reliable method was developed for isolating pollen nuclei from Brassica napus and Triticum aestivum for DNA analysis using flow cytometry. The nuclei were released from pollen by ultrasonic treatment. The isolated nuclei following filtration through nylon mesh and a purification procedure were suitable fo...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-004-0830-y

    authors: Pan G,Zhou Y,Fowke LC,Wang H

    更新日期:2004-10-01 00:00:00

  • Cation stimulation of the proton-translocating redox activity at the plasmalemma of Catharanthus roseus cells.

    abstract::Cultured Catharanthus roseus cells exhibit transmembrane ferricyanide reduction through a plasma membrane redox system which may be associated with proton translocation. Evidence shows that endogenous pyridine nucleotides serve as hydrogen donors for the reaction. The proton translocating function of the redox system ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00269886

    authors: Marigo G,Belkoura M

    更新日期:1985-12-01 00:00:00

  • Effect of promoter-leader sequences on transient expression of reporter gene chimeras biolistically transferred into sugarbeet (Beta vulgaris) suspension cells.

    abstract::Chimeric constructs consisting of the gus coding region fused downstream of promoterun-translated leader sequences from the tobacco osmotin and PR-S genes, the potato proteinase inhibitor 2 gene (pin2), and the cauliflower mosaic virus (CaMV) 35S promoter were biolistically transferred into sugarbeet suspension cells....

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00233152

    authors: Ingersoll JC,Heutte TM,Owens LD

    更新日期:1996-08-01 00:00:00

  • Chloroplast DNA synthesis in light and dark grown cultured Nicotiana tabacum cells as determined by molecular hybridization.

    abstract::A simple method using molecular hybridization was devised to quantitatively measure chloroplast DNA synthesis in vivo. Total cellular DNA isolated from Nicotiana tabacum suspension cells, labeled with (3)H-thymidine, was hybridized to nitrocellulose membrane-bound cloned chloroplast DNA (ct DNA) fragments. Colorless, ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00269202

    authors: Cannon G,Heinhorst S,Siedlecki J,Weissbach A

    更新日期:1985-04-01 00:00:00

  • Alfalfa MsCBL4 enhances calcium metabolism but not sodium transport in transgenic tobacco under salt and saline-alkali stress.

    abstract:KEY MESSAGE:MsCBL4 expression in tobacco enhanced its salt and saline-alkali stress tolerance by regulating calcium accumulation in roots, indicating the important role of calcium metabolism in plant saline-alkali stress tolerance The calcineurin B-like (CBL) family of proteins play important roles in plant abiotic str...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-020-02543-x

    authors: An Y,Yang XX,Zhang L,Zhang J,Du B,Yao L,Li XT,Guo C

    更新日期:2020-08-01 00:00:00

  • Promotion of flowering and morphological alterations in Atropa belladonna transformed with a CaMV 35S-rolC chimeric gene of the Ri plasmid.

    abstract::Kanamycin-resistant plants of belladonna (Atropa belladonna) were obtained after Agrobacterium mediated transformation. When a rolC gene, which is one of the loci located on Ri plasmid of Agrobacterium rhizogenes, was co-introduced with a kanamycin resistant (NPT II) gene under control of a cauliflower mosaic virus 35...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00232412

    authors: Kurioka Y,Suzuki Y,Kamada H,Harada H

    更新日期:1992-12-01 00:00:00

  • Chromosome elimination and mutation in tetraploid somatic hybrids ofSolanum tuberosum andSolanum phureja.

    abstract::The hybridity of eleven somatic hybrids between a diploidS. tuberosum and a diploidS. phureja clone could be verified because the parent karyotypes differed in their C-banding patterns. The hybrids were hypotetraploid and some carried structurally rearranged chromosomes and/or minute centric fragments. The nucleolar c...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00716844

    authors: Pijnacker LP,Ferwerda MA,Puite KJ,Schaart JG

    更新日期:1989-02-01 00:00:00

  • Plant cell cultures as food-aspects of sustainability and safety.

    abstract:KEY MESSAGE:Sustainability and safety aspects of plant cell cultures as food are presented. Applicability of dairy side streams as carbon source and use of natural growth enhancers in cultivation are shown. Biotechnologically produced cellular products are currently emerging to replace and add into the portfolio of agr...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-020-02592-2

    authors: Häkkinen ST,Nygren H,Nohynek L,Puupponen-Pimiä R,Heiniö RL,Maiorova N,Rischer H,Ritala A

    更新日期:2020-12-01 00:00:00

  • Plant regeneration from different explants in Helianthus smithii Heiser.

    abstract::Leaf, rhizome, and stem explants of Helianthus smithii, a wild relative of the cultivated sunflower, have been tested for their morphogenic potential on media containing a range of hormonal combinations including α-naphthaleneacetic acid and 6-benzylaminopurine (0-2 mg/l). Somatic embryos appeared on the majority of t...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s002990050303

    authors: Laparra H,Stoeva P,Ivanov P,Hahne G

    更新日期:1997-07-01 00:00:00

  • Variability in tissue cultures of Choisya ternata. Alkaloid accumulation in protoclones and aggregate clones obtained from established strains.

    abstract::Protoclones and aggregate clones have been prepared from 5 callus strains of C. ternata chosen for their dihydrofuroquinoline-accumulating capacities in a population of well-established strains. The results show that it is possible to obtain cell lines which accumulate higher alkaloid contents than the highest alkaloi...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00269565

    authors: Tremouillaux-Guiller J,Andreu F,Creche J,Chenieux JC,Rideau M

    更新日期:1987-10-01 00:00:00

  • Cloning and functional expression of the gene encoding an inhibitor against Aspergillus flavus alpha-amylase, a novel seed lectin from Lablab purpureus (Dolichos lablab).

    abstract::Maize is one of the more important agricultural crops in the world and, under certain conditions, prone to attack from pathogenic fungi. One of these, Aspergillus flavus, produces toxic and carcinogenic metabolites, called aflatoxins, as byproducts of its infection of maize kernels. The alpha-amylase of A. flavus is k...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-006-0250-2

    authors: Kim YH,Woloshuk CP,Cho EH,Bae JM,Song YS,Huh GH

    更新日期:2007-04-01 00:00:00

  • Transgenic papaya plants from Agrobacterium-mediated transformation of somatic embryos.

    abstract::Transgenic papaya (Carica papaya L.) plants were regenerated from embryogenic cultures that were cocultivated with a disarmed C58 strain of Agrobacterium tumefaciens containing one of the following binary cosmid vectors: pGA482GG or pGA482GG/cpPRV-4. The T-DNA region of both binary vectors includes the chimeric genes ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00237128

    authors: Fitch MM,Manshardt RM,Gonsalves D,Slightom JL

    更新日期:1993-03-01 00:00:00

  • A moso bamboo transcription factor, Phehdz1, positively regulates the drought stress response of transgenic rice.

    abstract:KEY MESSAGE:78 HD-Zip family genes in Phyllostachys edulis were analyzed. Overexpression of Phehdz1 can improve the drought tolerance of transgenic rice and affect its secondary metabolism. Many studies suggested homeodomain-leucine zipper (HD-Zip) transcription factors are important regulators of plant growth and deve...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-020-02625-w

    authors: Gao Y,Liu H,Zhang K,Li F,Wu M,Xiang Y

    更新日期:2021-01-01 00:00:00

  • Genetic transformation of Populus nigra by Agrobacterium tumefaciens.

    abstract::Two clones of Populus nigra L. were tested in vivo and in vitro for their susceptibility to three different Agrobacterium tumefaciens wild-type strains evaluating number and size of resulting calluses. Strain C58 proved to be the most virulent.Various parameters affecting Agrobacterium-mediated transformation of P. ni...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00233315

    authors: Confalonieri M,Balestrazzi A,Bisoffi S

    更新日期:1994-02-01 00:00:00

  • A simple and sensitive high-throughput GFP screening in woody and herbaceous plants.

    abstract::Green fluorescent protein (GFP) has been used widely as a powerful bioluminescent reporter, but its visualization by existing methods in tissues or whole plants and its utilization for high-throughput screening remains challenging in many species. Here, we report a fluorescence image analyzer-based method for GFP dete...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-008-0657-z

    authors: Hily JM,Liu Z

    更新日期:2009-03-01 00:00:00

  • A method for inflorescence proliferation.

    abstract::Most perennial plants must pass through a long juvenile phase of vegetative development before they are capable of flowering. We have developed a method specifying inflorescence proliferation to bypass juvenility and maintain the adult phase. Bamboo ( Bambusa edulis) inflorescences were amplified by incubation in Mura...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-003-0571-3

    authors: Lin CS,Chen CT,Lin CC,Chang WC

    更新日期:2003-06-01 00:00:00

  • Effects of agrobacterial oncogenes in kidney vetch (Anthyllis vulneraria L.).

    abstract::Kidney vetch seedlings were induced to form hairy roots by inoculating their mesocotyls with the wild-type strain 15834 of Agrobacterium rhizogenes or with the A. tumefaciens strain C58C1 containing a binary vector system (the pRiA4b as a helper and the vector pCB1346 bearing a pTiC58-derived isopentenyl transferase g...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00233367

    authors: Stiller J,Sinec VN,Svoboda S,Němcová B,Macháčková I

    更新日期:1992-07-01 00:00:00

  • Production of Nicotiana tabacum x Nicotiana acuminata hybrid by ovule culture.

    abstract::Using a conventional sexual crossing technique, Nicotiana tabacum x N. acuminata was not produced. After the fertilized ovules were cultured for 20 days in a liquid Nitsch H medium, germination was observed. The roots grew rapidly but leaves did not. However, plantlets were produced in an H medium containing Benzylade...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00268613

    authors: Iwai S,Kishi C,Nakata K,Kawashima N

    更新日期:1986-10-01 00:00:00