Abstract：
:The present protocol was aimed at establishing a routine transformation procedure via Agrobacterium tumefaciens for an important Oncidium orchid cultivar. An expression vector containing hptII and gusA genes driven by the cauliflower mosaic virus (CaMV) 35S promoter was successfully introduced into the Oncidium orchid genome by A. tumefaciens. Protocorm-like bodies (PLBs) derived from protocorms, were the target explants for transformation. The transformation was performed through two stages of cocultivation, the first stage occurring on antibiotic-free medium for 3 days, and the subsequent stage on medium containing 100 mg/l timentin for 1 month. Among 1,000 inoculated PLBs, 108 putatively transformed PLBs were proliferated on 5 mg/l hygromycin selection medium. A total of 28 independent transgenic orchid plants were obtained, from which six transgenic lines that were positive for beta-glucuronidase were randomly selected and confirmed by Southern, northern and western blot analyses. These results indicated that the foreign DNA was successfully integrated into the orchid genome and expressed transcriptionally and translationally in these orchid plants. The present transformation method reported is suitable for improving the Oncidium orchid through genetic engineering.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Liau CH,You SJ,Prasad V,Hsiao HH,Lu JC,Yang NS,Chan MTdoi
10.1007/s00299-003-0614-9keywords:
subject
Has Abstractpub_date
2003-06-01 00:00:00pages
993-8issue
10eissn
0721-7714issn
1432-203Xjournal_volume
21pub_type
杂志文章abstract：:Protoplasts isolated from cultured rice cells of an A-58 cytoplasmic male sterile line (A-58 MS)(Oryza sativa L.) were used to investigate the regeneration of rice plants. A cultured cell line (T3) of A-58 MS with a high growth rate and dense cytoplasm was selected. About 10% of the protoplasts prepared from this esta...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269240
更新日期：1986-04-01 00:00:00
abstract：:A genetic test was performed on seeds from 283 transgenic tobacco plants obtained by T-DNA transformation. Seeds from self-fertilized transgenic plants were germinated on kanamycin-containing medium, and the percentage of seeds which germinated, as well as the ratio of kanamycin-resistant to kanamycin-sensitive seedli...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00272760
更新日期：1988-12-01 00:00:00
abstract：:Scanning electron microscopy has been used to investigate the morphological changes occurring during the development of alfalfa somatic embryos. Embryos were initiated from callus, transferred to suspension culture and matured on solid agar medium. This developmental pattern was compared to that of zygotic embryos dev...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00235082
更新日期：1992-06-01 00:00:00
abstract：:A procedure has been developed for isolating protoplasts from prothalli of Ceratopteris richardii which can be cultured and are capable of regeneration. Protoplasts were isolated from 2-week-old gametophytes in a medium containing wall-digesting enzymes in 0.5 M sucrose, followed by purification of the released protop...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050470
更新日期：1998-06-01 00:00:00
abstract：KEY MESSAGE:FLA, the homolog of ubiquitin-specific protease does not have deubiquitination activity, but it is essential for flower and chloroplast development in rice. Ubiquitin-specific proteases (UBPs) are widely distributed and highly conserved proteins and are also members of the most important family of deubiquit...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-018-2368-4
更新日期：2019-03-01 00:00:00
abstract：: An effective method has been developed for the stable transformation and regeneration of Cavendish banana (Musa spp. AAA group) cv 'Grand Nain' by microprojectile bombardment. Embryogenic cell suspensions were initiated using immature male flowers as the explant. Cells were co-bombarded with the neomycin phosphotrans...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050004
更新日期：2000-01-01 00:00:00
abstract：:The human Bcl-xL gene was transformed into peanut cultivar Georgia Green via microprojectile bombardment. Following selection on hygromycin-containing medium and regeneration, eighty hygromycin-resistant callus clusters were recovered. Southern blot analysis of ten fertile lines revealed multiple insertions of the Bcl...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-007-0444-2
更新日期：2008-01-01 00:00:00
abstract：KEY MESSAGE:The zeaxanthin epoxidase gene ( MsZEP ) was cloned and characterized from alfalfa and validated for its function of tolerance toward drought and salt stresses by heterologous expression in Nicotiana tabacum. Zeaxanthin epoxidase (ZEP) plays important roles in plant response to various environment stresses d...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-015-1895-5
更新日期：2016-02-01 00:00:00
abstract：KEY MESSAGE:A novel rice constitutive promoter (P OsCon1 ) was isolated. The molecular mechanism of the promoter activity was investigated. P OsCon1 could be used as an alternative constitutive promoter for crop transgenic engineering. Monocot constitutive promoter is an important resource for crop transgenic engineeri...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-014-1644-1
更新日期：2014-10-01 00:00:00
abstract：:Polyclonal antibodies against a part of pea (Pisum sativum L.) LOXG protein have been raised to study the pattern of distribution of related lipoxygenases in pea carpels. The antiserum recognized three lipoxygenase polypeptides in carpels. One of them became undetectable 24 hours after fruit development induction, sug...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232465
更新日期：1996-04-01 00:00:00
abstract：:We have assessed the capacity of cultured protoplasts from two tissue sources of several commercially-grown broccoli cultivars to regenerate plants. A procedure that employs hypocotyl protoplasts and a culture medium with a high NAA:2,4-D auxin ratio was developed. The procedure permits highly efficient formation of c...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232858
更新日期：1990-10-01 00:00:00
abstract：:Plants were regenerated from leaf explants of Centrosema brasilianum cultured in vitro. Callus and buds were produced on Murashige and Skoog medium (MS), 0.8% agar, 0.1 mg/l NAA and 1 mg/l BAP. Regeneration of multiple shoots was achieved by transferring callus onto fresh medium containing 0.01 and 1 mg/l of NAA and B...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00236268
更新日期：1992-09-01 00:00:00
abstract：KEY MESSAGE:Our study shows that the expression of AtCBF3 and AtCOR15A improved the chilling tolerance in transgenic eggplant. In an attempt to improve chilling tolerance of eggplant (Solanum melongena L) plants, Arabidopsis C-repeat binding factor 3 (AtCBF3) and cold-regulated 15A (AtCOR15A) genes both driven by an Ar...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-014-1670-z
更新日期：2014-12-01 00:00:00
abstract：:The green fluorescent protein (GFP) from the jellyfish Aequorea victoria has proven to be a powerful tool in plant genetic transformation studies. This paper reviews the history and the progression of the expression of GFP variants in transgenic plants. The distinguishing features of the most useful GFPs, such as thos...
journal_title：Plant cell reports
pub_type： 杂志文章,评审
doi：10.1007/s002990100346
更新日期：2001-07-01 00:00:00
abstract：:Cultured Catharanthus roseus cells exhibit transmembrane ferricyanide reduction through a plasma membrane redox system which may be associated with proton translocation. Evidence shows that endogenous pyridine nucleotides serve as hydrogen donors for the reaction. The proton translocating function of the redox system ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269886
更新日期：1985-12-01 00:00:00
abstract：:A procedure for inducing somatic embryos in shoot apex explants (2 mm) excised from shoot proliferation cultures established from adult oak trees (Quercus robur) was investigated. Embryogenesis was induced in shoot tip as well as leaf explants in three out of the five genotypes evaluated. Somatic embryos were formed b...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-010-0852-6
更新日期：2010-06-01 00:00:00
abstract：KEY MESSAGE:Transcript profiling during the early induction phase of oil palm tissue culture and RNAi studies in a model somatic embryogenesis system showed that EgENOD93 expression is essential for somatic embryogenesis. Micropropagation of oil palm through tissue culture is vital for the generation of superior and un...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-020-02571-7
更新日期：2020-11-01 00:00:00
abstract：KEY MESSAGE:Transcriptomes of two switchgrass genotypes representing the upland and lowland ecotypes will be key tools in switchgrass genome annotation and biotic and abiotic stress functional genomics. Switchgrass (Panicum virgatum L.) is an important bioenergy feedstock for cellulosic ethanol production. We report ge...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-016-2065-0
更新日期：2017-01-01 00:00:00
abstract：:Pigeon pea [Cajanus cajan (L.) Millsp.] (Family: Fabaceae) is an important legume crop cultivated across 50 countries in Asia, Africa, and the Americas; and ranks fifth in area among pulses after soybean, common bean, peanut, and chickpea. It is consumed as a major source of protein (21%) to the human population in ma...
journal_title：Plant cell reports
pub_type： 杂志文章,评审
doi：10.1007/s00299-010-0899-4
更新日期：2010-10-01 00:00:00
abstract：KEY MESSAGE:Sugarcane lines transformed with an alanine aminotransferase gene demonstrated an improved nitrogen use efficiency compared with untransformed controls in a pot trial under low nitrogen conditions. ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-015-1768-y
更新日期：2015-05-01 00:00:00
abstract：:Maize is one of the more important agricultural crops in the world and, under certain conditions, prone to attack from pathogenic fungi. One of these, Aspergillus flavus, produces toxic and carcinogenic metabolites, called aflatoxins, as byproducts of its infection of maize kernels. The alpha-amylase of A. flavus is k...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-006-0250-2
更新日期：2007-04-01 00:00:00
abstract：:The DNA-binding domain of the yeast transcriptional activator GAL4 was expressed in transgenic tobacco plants in order to attempt to obtain specific repression of reporter genes. Expression of the GUS and the NPTII gene was essentially the same regardless of the presence of a gene encoding a functional GAL4 DNA-bindin...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232920
更新日期：1995-10-01 00:00:00
abstract：KEY MESSAGE:Prolamin-GFP fusion proteins, expressed under the control of native prolamin promoters, were localized in specific layers of PB-Is. Prolamin-GFP fusion proteins were gradually digested from outside by pepsin digestion. In rice seed endosperm, protein body type I (PB-I) has a layered structure consisting of ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-016-1960-8
更新日期：2016-06-01 00:00:00
abstract：:Responses induced by Cd exposure were assessed in tobacco seedlings (Nicotiana tabacum L.) using macro and molecular indices. The 100 microM of Cd exposure reduced the total dry weight and chlorophyll index of the seedlings as much as the genuine Fe-deficiency. Concentration of Fe in the shoots decreased, whereas that...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-005-0092-3
更新日期：2006-04-01 00:00:00
abstract：:Protoplasts were isolated from embryogenic callus ofAbies alba L. which originated from immature seeds. The protoplasts were immobilized in alginate beads in order to follow the development of single protoplasts.Surrounding culture medium was modified from Kao and Michayluk (1975). After cell wall regeneration subsequ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00716854
更新日期：1989-02-01 00:00:00
abstract：:Compact, friable and embryogenic calli were initiated from immature inflorescences and young leaf bases of one week old seedlings of Paspalum scrobiculatum cultured on MS medium supplemented with 2,4-D. A stable, embryogenic suspension culture was initiated from these calli and maintained in a liquid version of the sa...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00274134
更新日期：1989-05-01 00:00:00
abstract：:Expression of the green fluorescent protein (gfp) gene, under regulatory control of either the constitutive 35S promoter or the developmentally-regulated lectin promoter was monitored and quantified using a newly-developed automated tracking system. The automated system consisted of a computer-controlled two-dimension...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-006-0142-5
更新日期：2006-09-01 00:00:00
abstract：:Stem sections from shoot cultures maintained in vitro were used to produce transgenic plants of the potato, Solanum tuberosum L. cv. 'Russet Burbank'. Stem internode pieces inoculated with Agrobacterium tumefaciens containing coat protein genes from potato virus X and potato virus Y, produced shoots with a frequency o...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00233028
更新日期：1991-05-01 00:00:00
abstract：:High yields of protoplasts were obtained from leaves of aseptically grown plants and calli originated from different explants, in several cultivars of Cajanus cajan L. The protoplasts divided to form cell clusters in modified KM 8p medium and developed to protocolonies after dilution with liquid Caboche's medium withi...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232691
更新日期：1992-08-01 00:00:00
abstract：:Embryogenic cell suspension cultures were established from Triticum aestivum X Leymus angustus F1 hybrids, using compact nodular calli derived from inflorescence segments. Calli originating from leaf segments did not give rise to stable cell suspensions. Growth measurements of the cell suspensions revealed that they c...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00235258
更新日期：1992-03-01 00:00:00
