Genetic analysis of T-DNA insertions into the tobacco genome.

Abstract:

:A genetic test was performed on seeds from 283 transgenic tobacco plants obtained by T-DNA transformation. Seeds from self-fertilized transgenic plants were germinated on kanamycin-containing medium, and the percentage of seeds which germinated, as well as the ratio of kanamycin-resistant to kanamycin-sensitive seedlings were scored. Nine categories of transformants could be distinguished according to the number of loci into which T-DNA had inserted, and according to the effects of T-DNA integration on seed or seedling development. In most of the plants, T-DNA was inserted into a single site; others contained multiple independent copies of T-DNA. The number of T-DNA integration sites was found to be independent of whether a binary vector system or a cointegrate Ti plasmid had been used to obtain the transgenic plant. Loss of marker genes or marker gene expression from generation to generation appeared to be a quite frequent event. Plants which appeared to be insertional recessive embryo-lethal mutants did not exhibit this trait in the next generation.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Heberle-Bors E,Charvat B,Thompson D,Schernthaner JP,Barta A,Matzke AJ,Matzke MA

doi

10.1007/BF00272760

subject

Has Abstract

pub_date

1988-12-01 00:00:00

pages

571-4

issue

7

eissn

0721-7714

issn

1432-203X

journal_volume

7

pub_type

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