Development of a cyclic somatic embryogenesis regeneration system for leek (Allium ampeloprasum L.) using zygotic embryos.

Abstract:

:In leek (Allium ampeloprasum L.) a cyclic system of somatic embryogenesis was developed. Somatic embryos used for cyclic embryogenesis were able to develop the same type of embryogenic callus as zygotic embryos in the primary cycle. For the first time a comparison of the efficiencies of both expiants was made. Ten families were investigated for somatic embryogenesis. There was a genetic relationship with respect to somatic embryo production between the reciprocal crosses. From each family one genotype was selected for investigating cyclic somatic embryogenesis. Different levels of somatic embryo production were found between the expiants of zygotic and somatic embryos. The two best genotypes, 92.001-03 and 92.002-33 produced twice as many somatic embryos as the overall average. On average, 56% of the somatic embryos finally developed into greenhouse plantlets.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Schavemaker CM,Jacobsen E

doi

10.1007/BF00233638

subject

Has Abstract

pub_date

1995-01-01 00:00:00

pages

227-31

issue

4

eissn

0721-7714

issn

1432-203X

journal_volume

14

pub_type

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