Abstract:
:Highly viable protoplasts were isolated in large numbers from in vitro-grown leaf and stem tissues of a haploid clone of the apple scion cultivar Golden Delicious (Malus Xdomestica Borkh.). Protoplasts from both sources divided rapidly to give microcallus, when cultured in a modified Kao and Michayluk-based medium. Following two successive subcultures for callusing, shoot buds were regenerated from such calli, on half-strength Murashige and Skoog medium with an increased concentration of group B vitamins and containing 5.0 mg.l(-1) 6-benzyl-aminopurine and 0.1 mg.l(-1) l-naphthaleneacetic acid (for the leaf protoplast-derived calli) or 4-indole-3yl-butyric acid (for stem protoplast-derived calli). The mesophyll protoplast-derived shoots were enfeebled and vitrified, in time with their ultimate death. Conversely, for those shoots deriving from the stem protoplasts, in vitro propagation was successfully achieved. This is the first report on the successful isolation, culture and organogenesis from stem protoplasts of a woody plant genotype.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Patat-Ochatt EM,Boccon-Gibod J,Duron M,Ochatt SJdoi
10.1007/BF00241946subject
Has Abstractpub_date
1993-01-01 00:00:00pages
118-20issue
2eissn
0721-7714issn
1432-203Xjournal_volume
12pub_type
杂志文章abstract:KEY MESSAGE:FLA, the homolog of ubiquitin-specific protease does not have deubiquitination activity, but it is essential for flower and chloroplast development in rice. Ubiquitin-specific proteases (UBPs) are widely distributed and highly conserved proteins and are also members of the most important family of deubiquit...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-018-2368-4
更新日期:2019-03-01 00:00:00
abstract::This study aimed at establishing a protocol to increase the number of regenerated shoots and to limit the recovery of "escapes" during the regeneration of transgenic flax plants (cv Barbara). Here, we describe how light, adapted media and selection scheme could stimulate the transformation process, the organogenic pot...
journal_title:Plant cell reports
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abstract::Genistein, 4',5,7-trihydroxyisoflavone, is an isoflavonoid compound predominantly restricted to legumes and known to possess phyto-oestrogenic and antioxidative activities. The key enzyme that redirects phenylpropanoid pathway intermediates from flavonoids to isoflavonoids is the isoflavone synthase (IFS). Brassica na...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1052-8
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abstract::Ethylene emanation rates were assessed from leaf tissues of an embryogenic seed plant (Cycle 0) and regeneration cycle plants selected for enhanced embryogenesis (Cycles I, II and IV). In all experiments, ethylene was assessed from the basal 1 cm portion of the innermost leaf. Ethylene emanation was five-fold higher i...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00272059
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abstract::In this opinion article, we briefly review the status of crop biotechnology research-with emphasis on the development of GM crops-in Jamaica and Venezuela. We focus on the transgenic papayas developed for both countries, and examine the factors hindering not only the development and application of this biotechnologica...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-010-0988-4
更新日期:2011-05-01 00:00:00
abstract::A protocol previously developed for B. napus microspore culture was modified to produce embryos from several lines of Brassica campestris. Bud size, genotype, media constituents, and incubation time and temperature were examined. Donor plants were grown in a growth cabinet at a day/night temperature of 10/5°C. Microsp...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00235072
更新日期:1992-06-01 00:00:00
abstract:KEY MESSAGE:A novel Zoysia japonica salt-induced glycine-rich RNA-binding protein gene was cloned in this study and its overexpression caused salt sensitivity in transgenic Arabidopsis. Glycine-rich RNA-binding proteins (GRPs) play crucial roles in diverse plant developmental processes. However, the mechanisms and func...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-016-2068-x
更新日期:2017-01-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269934
更新日期:1988-08-01 00:00:00
abstract::KEY MESSAGE : Rooting of Artemisia annua increases trichome size on leaves and helps drive the final steps of the biosynthesis of the sesquiterpene antimalarial drug, artemisinin. ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-012-1355-4
更新日期:2013-02-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-019-02482-2
更新日期:2020-01-01 00:00:00
abstract:KEY MESSAGE:This study established an efficient method of regenerating plants of Ficus lyrata and producing purple-leaved F. lyrata plants through genetic transformation using a VvMybA1 gene of grapevine. ABSTRACT:Ficus lyrata, a species with unique violin- or guitar-shaped leaves, was regenerated from leaf-derived ca...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-013-1491-5
更新日期:2013-11-01 00:00:00
abstract::Eustoma grandiflorum Shinn requires vernalization for the induction of stem elongation and flowering. To investigate the role of gibberellins (GAs) in vernalization, the expression levels of genes encoding enzymes of GA biosynthesis, copalyl diphosphate synthetase, GA 20-oxidase and GA 3 beta-hydroxylase, were examine...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0672-z
更新日期:2003-09-01 00:00:00
abstract::Chimeric constructs consisting of the gus coding region fused downstream of promoterun-translated leader sequences from the tobacco osmotin and PR-S genes, the potato proteinase inhibitor 2 gene (pin2), and the cauliflower mosaic virus (CaMV) 35S promoter were biolistically transferred into sugarbeet suspension cells....
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233152
更新日期:1996-08-01 00:00:00
abstract::Maize is one of the more important agricultural crops in the world and, under certain conditions, prone to attack from pathogenic fungi. One of these, Aspergillus flavus, produces toxic and carcinogenic metabolites, called aflatoxins, as byproducts of its infection of maize kernels. The alpha-amylase of A. flavus is k...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-006-0250-2
更新日期:2007-04-01 00:00:00
abstract::We have developed a plant- Escherichia coli pASV shuttle vector from the essential elements of the Ageratum yellow vein virus (AYVV). The geminivirus vector contains the AYVV genome with the coat-protein deletion, the E. coli vector backbone of pUC19, a unique cloning site and gene expression cassettes for plant selec...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-004-0792-0
更新日期:2004-08-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-013-1427-0
更新日期:2013-08-01 00:00:00
abstract::Plant regeneration via somatic embryogenesis was achieved in callus cultures derived from nodal explants of in vitro grown seedlings and excised mature zygotic embryos of three bamboo species on Murashige and Skoog's (MS) basal medium supplemented with 0.5 mg/l kinetin (Kn), 2.0 mg/l 2,4-dichlorophenoxyacetic acid (2,...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00231624
更新日期:1994-09-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0614-9
更新日期:2003-06-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-020-02564-6
更新日期:2020-10-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-006-0176-8
更新日期:2006-11-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-009-0756-5
更新日期:2009-10-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00234579
更新日期:1991-12-01 00:00:00
abstract::While searching for genes expressed in acid lemon but not in acidless lime pulp, we isolated clone Cl111 which showed the following expression phenotypes: (1) while it was expressed in the ovaries in both varieties, its mRNA was detected only in the pulp of the acid fruit, (2) no or very low expression of the gene was...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1073-3
更新日期:2011-09-01 00:00:00
abstract::A transformed root clone of Datura candida×D. aurea was established following infection with Agrobacterium rhizogenes strain A4. This clone was examined for its growth and hyoscyamine and scopolamine content under various culture conditions. Among the three basal culture media tested, half-strength Gamborg's B5 medium...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050415
更新日期:1998-03-01 00:00:00
abstract::Quantitative trait loci (QTLs) associated with androgenic responsiveness in triticale were analyzed using a population of 90 DH lines derived from the F1 cross between inbred line 'Saka 3006' and cv. 'Modus', which was used in a number of earlier studies on molecular mapping in this crop. Using Windows QTL Cartographe...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-012-1320-2
更新日期:2012-11-01 00:00:00
abstract::Shoot cultures of Allium sativum L. were established from stem-tip and scape-tip. Various combinations of media, growth regulators were compared. The best establishment and proliferation of stem-tip and scape-tip explants were obtained on MS medium containing 0.5 mg l(-1) NAA and 0.5 mg l(-1) KT. The roots were produc...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233301
更新日期:1994-11-01 00:00:00
abstract::In order to construct transgenic rice plant with an introduced oryzacystatin (OC)-β-glucuronidase (GUS) fusion gene, we first introduced it into rice protoplasts by electroporation, together with a marker gene conferring hygromycinresistance (pUC-HPH). In a transient assay using the transfected protoplasts, both OC an...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00193714
更新日期:1995-12-01 00:00:00
abstract::Two modes of endocytosis are known to occur in eucaryotic cells: fluid phase and receptor-mediated endocytosis. Fluid-phase endocytosis in plant cells resembles the retrieval of excess plasma membrane material previously incorporated by exocytosis. Pollen tubes need to carry out strong membrane retrieval due to their ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-007-0437-1
更新日期:2008-01-01 00:00:00
abstract::The first enzyme in the flavonoid pathway, chalcone synthase, is encoded by a gene (CHS) whose expression is normally under developmental control. In our previous studies, an 896-bp promoter region of a flower-specific CHS gene was isolated from Lilium orential 'Sorbonne', and designated as PLoCHS. Here, the PLoCHS pr...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1124-9
更新日期:2011-12-01 00:00:00
abstract::Mesophyll derived protoplasts of Solanum khasianum Clarke and S. aculeatissimum Jacq. were fused by electrofusion and resulted in interspecific hybrids. In total, 249 calluses were obtained from which more than 65% produced shoots. From the regenerated plantlets 45% could be identified as hybrids by chromosome counts ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00239891
更新日期:1994-01-01 00:00:00