Organogenesis of stem and leaf protoplasts of a haploid golden delicious apple clone (Malus Xdomestica Borkh.).

Abstract:

:Highly viable protoplasts were isolated in large numbers from in vitro-grown leaf and stem tissues of a haploid clone of the apple scion cultivar Golden Delicious (Malus Xdomestica Borkh.). Protoplasts from both sources divided rapidly to give microcallus, when cultured in a modified Kao and Michayluk-based medium. Following two successive subcultures for callusing, shoot buds were regenerated from such calli, on half-strength Murashige and Skoog medium with an increased concentration of group B vitamins and containing 5.0 mg.l(-1) 6-benzyl-aminopurine and 0.1 mg.l(-1) l-naphthaleneacetic acid (for the leaf protoplast-derived calli) or 4-indole-3yl-butyric acid (for stem protoplast-derived calli). The mesophyll protoplast-derived shoots were enfeebled and vitrified, in time with their ultimate death. Conversely, for those shoots deriving from the stem protoplasts, in vitro propagation was successfully achieved. This is the first report on the successful isolation, culture and organogenesis from stem protoplasts of a woody plant genotype.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Patat-Ochatt EM,Boccon-Gibod J,Duron M,Ochatt SJ

doi

10.1007/BF00241946

subject

Has Abstract

pub_date

1993-01-01 00:00:00

pages

118-20

issue

2

eissn

0721-7714

issn

1432-203X

journal_volume

12

pub_type

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