Opportunities and constraints to biotechnological applications in the Caribbean: transgenic papayas in Jamaica and Venezuela.

abstract：:Rice plants (Oryza sativa L., Chinsurah Boro II var. Indica) were regenerated from protoplasts isolated from microspore derived cell suspensions. A simple procedure for the establishment of such cell suspension cultures from embryogenic microcallus derived from cultured isolated microspores of Indica-type rice is desc...

journal_title：Plant cell reports

authors： Datta SK,Datta K,Potrykus I

更新日期：1990-09-01 00:00:00

abstract：:A T-DNA insertional mutant OsPE of rice gives twin and triplet seedlings in up to 20 % of the seeds. Detailed cytological and histological analysis of OsPE indicated normal male and female gametogenesis in the OsPE mutant. Confocal laser scanning microscopic (CLSM) analysis of the developing seeds of OsPE showed multi...

journal_title：Plant cell reports

authors： Paul P,Awasthi A,Kumar S,Verma SK,Prasad R,Dhaliwal HS

更新日期：2012-10-01 00:00:00

abstract：KEY MESSAGE:Our study shows that the expression of AtCBF3 and AtCOR15A improved the chilling tolerance in transgenic eggplant. In an attempt to improve chilling tolerance of eggplant (Solanum melongena L) plants, Arabidopsis C-repeat binding factor 3 (AtCBF3) and cold-regulated 15A (AtCOR15A) genes both driven by an Ar...

journal_title：Plant cell reports

authors： Wan F,Pan Y,Li J,Chen X,Pan Y,Wang Y,Tian S,Zhang X

更新日期：2014-12-01 00:00:00

abstract：:LITHOSPERMUM ERYTHRORHIZON: , which are capable of producing red pigments, have been established. The red pigments were formed on the stems of L. erythrorhizon shoots cultured both on solid and in liquid media without phytohormones at 25 °C in the dark. Thin-layer chromatography, high-performance liquid chromatography...

journal_title：Plant cell reports

authors： Touno K,Harada K,Yoshimatsu K,Yazaki K,Shimomura K

更新日期：2000-11-01 00:00:00

abstract：:Plum hypocotyl slices were transformed with the coat protein (CP) gene of plum pox virus (PPV-CP) following cocultivation with Agrobacterium tumefaciens containing the plasmid pGA482GG/PPVCP-33. This binary vector carries the PPV-CP gene construct, as well as the chimeric neomycin phosphotransferase and β-glucuronidas...

journal_title：Plant cell reports

authors： Scorza R,Ravelonandro M,Callahan AM,Cordts JM,Fuchs M,Dunez J,Gonsalves D

更新日期：1994-11-01 00:00:00

abstract：:As part of an ongoing research program dedicated to the understanding of proanthocyanidin (PA) accumulation in Brassica napus seed coat, transgenic rapeseed plants carrying a 2.3-kb fragment of the Arabidopsis thaliana BAN promoter (ProAtBAN) fused to the uidA reporter gene (GUS) were generated. Analysis of these plan...

journal_title：Plant cell reports

authors： Nesi N,Lucas MO,Auger B,Baron C,Lécureuil A,Guerche P,Kronenberger J,Lepiniec L,Debeaujon I,Renard M

更新日期：2009-04-01 00:00:00

abstract：: St. John's wort (Hypericum perforatum. cv 'Anthos') is a medicinal plant with evidence of efficacy as an anti-depressant. The present report describes the development of an in vitro regeneration system that utilizes thidiazuron [N-phenyl-N'-(1,2,3-thidiazol-yl)urea] for the induction of de novo shoots on etiolated hy...

journal_title：Plant cell reports

authors： Murch SJ,Choffe KL,Victor JMR,Slimmon TY,KrishnaRaj S,Saxena PK

更新日期：2000-05-01 00:00:00

abstract：:Embryogenic cell suspension cultures were established from Triticum aestivum X Leymus angustus F1 hybrids, using compact nodular calli derived from inflorescence segments. Calli originating from leaf segments did not give rise to stable cell suspensions. Growth measurements of the cell suspensions revealed that they c...

journal_title：Plant cell reports

authors： Tabaeizadeh Z,Campeau N

更新日期：1992-03-01 00:00:00

abstract：:Leaf explants ofKalanchoe laciniata were cocultivated for different days (2, 4, 6 and 8 days) with disarmedAgrobacterium tumefaciens strains A208SE, GV3111SE and EHA101 carring a binary vector pROA93. The vector contains a cauliflower mosaic virus 35S promotor which drives the coding sequence of neomycin phosphotransf...

journal_title：Plant cell reports

authors： Jia SR,Yang MZ,Ott R,Chua NH

更新日期：1989-06-01 00:00:00

abstract：:Microspore-derived embryos from Brassica napus L. were dried to less than 15% moisture and stored dry for a minimum of 7 days. Successful plant regeneration was observed when embryos at the cotyledonary stage of development were treated with 50 uM ABA for 7 days prior to desiccation. Solid agar or liquid medium gave s...

journal_title：Plant cell reports

authors： Senaratna T,Kott L,Beversdorf WD,McKersie BD

更新日期：1991-09-01 00:00:00

abstract：:A novel genic male sterile (GMS) line in Brassica napus L., which was identified in 1999, was found to be controlled by a monogenic dominant gene, which we have designated as MDGMS. The microspores of the MDGMS abort before the degradation of the tapetal cell layer. The F1 fertility from any fertile lines crossed with...

journal_title：Plant cell reports

authors： Wang DJ,Guo AG,Li DR,Tian JH,Huang F,Sun GL

更新日期：2007-05-01 00:00:00

abstract：:The nuclear DNA content of sweet pepper (Capsicum annuum L. var. annuum, 2n = 24) has been measured by flow and image cytometries but the DNA content of each chromosome of this species has not yet been regarded. DNA content of individual chromosomes has been quantified by the flow karyotyping technique, which requires...

journal_title：Plant cell reports

authors： de Abreu IS,Carvalho CR,Clarindo WR

更新日期：2008-07-01 00:00:00

abstract：:In the present paper, attempts were made to explore the possibility of employing ultraviolet (UV) irradiation in citrus asymmetric fusion for transfer of limited amount of favorable traits from a desirable cultivar to a target one. Exposure of Satsuma mandarin (Citrus unshiu Marc.) embryogenic protoplasts to UV at an ...

journal_title：Plant cell reports

authors： Xu XY,Hu ZY,Li JF,Liu JH,Deng XX

更新日期：2007-08-01 00:00:00

abstract：:Callus cultures of Vitis labruscana cv. Concord were initiated from both immature fruit and petiole tissue. Analysis revealed that the acid hydrolyzable precursors to β-damascenone, a compound important to the flavor of most grape species, were present in both callus types. Light and increased sucrose concentration we...

journal_title：Plant cell reports

authors： Shure KB,Acree TE

更新日期：1994-05-01 00:00:00

abstract：:Protoclones and aggregate clones have been prepared from 5 callus strains of C. ternata chosen for their dihydrofuroquinoline-accumulating capacities in a population of well-established strains. The results show that it is possible to obtain cell lines which accumulate higher alkaloid contents than the highest alkaloi...

journal_title：Plant cell reports

authors： Tremouillaux-Guiller J,Andreu F,Creche J,Chenieux JC,Rideau M

更新日期：1987-10-01 00:00:00

abstract：:Valencia orange [Citrus sinensis (L.) Osbeck] is the leading commercial citrus species in the world for processed juice products; however, the presence of thermostable pectin methylesterase (TSPME) reduces its juice quality. A long-term strategy of this work is to eliminate or greatly reduce TSPME activity in Valencia...

journal_title：Plant cell reports

authors： Guo W,Duan Y,Olivares-Fuster O,Wu Z,Arias CR,Burns JK,Grosser JW

更新日期：2005-10-01 00:00:00

abstract：:DNA fingerprints generated by the Jeffreys' probes, 33.6 and 33.15, indicated the presence of minisatellite-like sequences in the red clover genome. The fingerprints generated by probe 33.6 gave less background and fewer but better defined bands than those obtained with probe 33.15. Assay of a regenerative somaclonal ...

journal_title：Plant cell reports

authors： Nelke M,Nowak J,Wright JM,McLean NL

更新日期：1993-12-01 00:00:00

abstract：:To develop an Agrobacterium mediated transformation protocol for chrysanthemum we studied the transformation efficiency of commonly used A.tumefaciens strains on 14 genotypes by comparing tumour size and frequency. One genotype was analyzed in detail using 14 strains of both A.tumefaciens and A.rhizogenes. Only a few ...

journal_title：Plant cell reports

authors： van Wordragen MF,de Jong J,Huitema HB,Dons HJ

更新日期：1991-01-01 00:00:00

abstract：:To develop an alternative genetic transformation system that is not dependent on an antibiotic selection strategy, the phosphomannose isomerase gene (pmi) system was evaluated for producing transgenic plants of chickpea (Cicer arietinum L.). A shoot morphogenesis protocol based on the thidiazuron (TDZ)-induced shoot m...

journal_title：Plant cell reports

authors： Patil G,Deokar A,Jain PK,Thengane RJ,Srinivasan R

更新日期：2009-11-01 00:00:00

abstract：:The expression of green fluorescent protein (GFP) and its inheritance were studied in transgenic oat ( Avena sativa L.) plants transformed with a synthetic green fluorescent protein gene [sgfp(S65T)] driven by a rice actin promoter. In vitro shoot meristematic cultures (SMCs) induced from shoot apices of germinating m...

journal_title：Plant cell reports

authors： Cho MJ,Choi HW,Okamoto D,Zhang S,Lemaux PG

更新日期：2003-01-01 00:00:00

abstract：:Highly viable protoplasts were isolated in large numbers from in vitro-grown leaf and stem tissues of a haploid clone of the apple scion cultivar Golden Delicious (Malus Xdomestica Borkh.). Protoplasts from both sources divided rapidly to give microcallus, when cultured in a modified Kao and Michayluk-based medium. Fo...

journal_title：Plant cell reports

authors： Patat-Ochatt EM,Boccon-Gibod J,Duron M,Ochatt SJ

更新日期：1993-01-01 00:00:00

abstract：:Late Embryogenesis Abundant (LEA) proteins are associated with tolerance to water-related stress. A wheat (Triticum durum) group 2 LEA proteins, known also as dehydrin (DHN-5), has been previously shown to be induced by salt and abscisic acid (ABA). In this report, we analyze the effect of ectopic expression of Dhn-5 ...

journal_title：Plant cell reports

authors： Brini F,Hanin M,Lumbreras V,Amara I,Khoudi H,Hassairi A,Pagès M,Masmoudi K

更新日期：2007-11-01 00:00:00

abstract：:A reliable protocol for the transformation of cultivated sunflower (Helianthus annuus L.) has been established, based on microprojectile bombardment of half shoot apices in combination with Agrobacterium tumefaciens coculture. Transgenic shoots have been obtained from 5 inbred lines, although transformation efficienci...

journal_title：Plant cell reports

authors： Knittel N,Gruber V,Hahne G,Lénée P

更新日期：1994-12-01 00:00:00

abstract：:In vitro plant regeneration via embryogenesis was obtained in suspension cultures of Datura innoxia Mill. Embryogenesis was induced in suspension cultures raised from callus of androgenetic origin, using LS liquid medium supplemented with 0.22 mg/l 2,4-D. The total number of embryos formed was variable over time in cu...

journal_title：Plant cell reports

authors： Sharma VK,Jethwani V,Kothari SL

更新日期：1993-08-01 00:00:00

abstract：:The transit peptide sequence of ibAGP2 (TP2) was found to be capable of targeting protein into the chloroplast in the Arabidopsis protoplasts. TP2 was fused to a beta-glucuronidase (GUS) reporter gene and expressed in Arabidopsis under the control of the ibAGP2 promoter with the aim of dissecting the effect of the tra...

journal_title：Plant cell reports

authors： Kwak MS,Oh MJ,Paek KH,Shin JS,Bae JM

更新日期：2008-08-01 00:00:00

abstract：:Developmental biology studies in general benefit from model organisms that are well characterized. Arabidopsis thaliana fulfills this criterion and represents one of the best experimental systems to study developmental processes in higher plants. Light is a crucial factor that drives photosynthesis, but that also regu...

journal_title：Plant cell reports

authors： Boron AK,Vissenberg K

更新日期：2014-05-01 00:00:00

abstract：:Three O-methyltransferases which catalyze S-adenosyl-L-methionine (SAM)-dependent O-methylation of licodione (LMT), flavone/flavonol (FMT), and caffeic acid (CMT) were separated from the callus culture of Glycyrrhiza echinata, and characteristic differences between their pH optima and Mg(2+) requirement for activity w...

journal_title：Plant cell reports

authors： Ayabe S,Udagawa A,Iida K,Yoshikawa T,Furuya T

更新日期：1987-02-01 00:00:00

abstract：KEY MESSAGE:We isolated differentially expressed and dark-responsive genes during flower development and opening in petals of morning glory. Flower opening usually depends on petal expansion and is regulated by both genetic and environmental factors. Flower opening in morning glory (Ipomoea nil) is controlled by the da...

journal_title：Plant cell reports

authors： Shinozaki Y,Tanaka R,Ono H,Ogiwara I,Kanekatsu M,van Doorn WG,Yamada T

更新日期：2014-07-01 00:00:00

abstract：KEY MESSAGE:We provide evidence that nucleotide sequence and methylation status changes occur in the Arabidopsis genome during in vitro tissue culture at a frequency high enough to represent an important source of variation. Somaclonal variation is a general consequence of the tissue culture process that has to be anal...

journal_title：Plant cell reports

authors： Coronel CJ,González AI,Ruiz ML,Polanco C

更新日期：2018-01-01 00:00:00

abstract：:An early identification of fusion products was based on the presumed vigorous growth of hybrid calluses after fusion between Solanum brevidens and S. tuberosum leaf protoplasts. The S. brevidens protoplasts were unable to form multicellular colonies under the applied culture conditions. Three size groups of calluses w...

journal_title：Plant cell reports

authors： Polgár Z,Preiszner J,Dudits D,Fehér A

更新日期：1993-05-01 00:00:00