Abstract：
:Transgenic herbicide tolerant Acacia sinuata plants were produced by transformation with the bar gene conferring phosphinothricin resistance. Precultured hypocotyl explants were infected with Agrobacterium tumefaciens strain EHA105 in the presence of 100 microM acetosyringone and shoots regenerated on MS (Murashige and Skoog, 1962, Physiol Plant 15:473-497) medium with 13.3 microM benzylaminopurine, 2.6 microM indole-3-acetic acid, 1 g l(-1) activated charcoal, 1.5 mg l(-1) phosphinothricin, and 300 mg l(-1) cefotaxime. Phosphinothricin at 1.5 mg l(-1) was used for the selection. Shoots surviving selection on medium with phosphinothricin expressed GUS. Following Southern hybridization, eight independent shoots regenerated of 500 cocultivated explants were demonstrated to be transgenic, which represented transformation frequency of 1.6%. The transgenics carried one to four copies of the transgene. Transgenic shoots were propagated as microcuttings in MS medium with 6.6 microM 6-benzylaminopurine and 1.5 mg l(-1) phosphinothricin. Shoots elongated and rooted in MS medium with gibberellic acid and indole-3-butyric acid, respectively both supplemented with 1.5 mg l(-1) phosphinothricin. Micropropagation of transgenic plants by microcuttings proved to be a simple means to bulk up the material. Several transgenic plants were found to be resistant to leaf painting with the herbicide Basta.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Vengadesan G,Amutha S,Muruganantham M,Anand RP,Ganapathi Adoi
10.1007/s00299-006-0176-8subject
Has Abstractpub_date
2006-11-01 00:00:00pages
1174-80issue
11eissn
0721-7714issn
1432-203Xjournal_volume
25pub_type
杂志文章abstract：:The effects of serial combinations of either indole-3-acetic acid, indole-3-butyric acid or α-naphthaleneacetic acid (0.5-10.0 mg/l) with either kinetin, 6-benzyl-amino-purine, zeatin or 6-methylaminopurine (0.5-5.0 mg/l) have been investigated to assess the morphogenetic potential of foliar explants of Duboisia myopo...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269711
更新日期：1986-02-01 00:00:00
abstract：:The aim of this study was the improvement of embryo production in wheat anther culture. Three butanol alcohols, n-butanol, sec-butanol and tert-butanol, were evaluated for their effect on microspore embryogenesis in two spring cultivars of wheat, Pavon and Caramba. Application of n-butanol, at 0.1 and 0.2% (v/v) in th...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-007-0500-y
更新日期：2008-05-01 00:00:00
abstract：:A reliable plant regeneration system is described for the production of adventitious shoots from root explants of spinach. Explants from roots of axenic shoots and roots induced on cultured hypocotyl explants were used for adventitious shoot induction. Explants from apical, middle and basal root regions were incubated...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050359
更新日期：1997-12-01 00:00:00
abstract：:An efficient micropropagation system for Saussurea involucrata Kar. et Kir., an endangered Chinese medicinal plant, has been developed. Shoot organogenesis occurred from S. involucrata leaf explants inoculated on medium with appropriate supplements of plant growth regulators. 66.0% of shoot regeneration frequency and ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-006-0230-6
更新日期：2007-03-01 00:00:00
abstract：:We report here an efficient Arabidopsis leafdisc transformation protocol yielding an average transformation frequency of 1.6 transgenic shoots per leaf explant 4 weeks after the bacterial infection period. Subsequent cultivation in vitro is such that a high percentage (85-90%) of the primary transformants produces see...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232455
更新日期：1996-04-01 00:00:00
abstract：:Root vigor is an important trait for the growth of terrestrial plants, especially in water-deficit environments. Although deserts plants are known for their highly developed root architecture, the molecular mechanism responsible for this trait has not been determined. Here we established an efficient protocol for the ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-010-0863-3
更新日期：2010-07-01 00:00:00
abstract：KEY MESSAGE:The first Good Manufacturing Practices production of a purification-free rice-based oral cholera vaccine (MucoRice-CTB) from transgenic plants in a closed cultivation system yielded a product meeting regulatory requirements. Despite our knowledge of their advantages, plant-based vaccines remain unavailable ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-015-1911-9
更新日期：2016-03-01 00:00:00
abstract：KEY MESSAGE:A chalcone synthase gene ( PaCHS ) was isolated and functionally characterized from liverwort. The ectopic expression of PaCHS in Marchantia paleacea callus raised the flavonoids content. Chalcone synthase (CHS; EC 2.3.1.74) is pivotal for the biosynthesis of flavonoid and anthocyanin pigments in plants. It...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-014-1702-8
更新日期：2015-02-01 00:00:00
abstract：:Vitrification is a technically simple method for cryopreserving plant germplasm, requiring only the application of suitable cryoprotectants and rapid cooling rates. Sweetpotato (Ipomoea batatas [L.] Lam.) shoot tips obtained from in vitro plants survived liquid nitrogen (-196°C) exposure following a vitrification-indu...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232527
更新日期：1992-05-01 00:00:00
abstract：:High yields of viable protoplasts were obtained by enzymatic treatment from cotyledonary leaves of various greenhouse grown Phaseolus vulgaris L. cultivars. The protoplasts divided and formed cell clusters in a liquid medium. Early transfer before 10 days in the same medium was necessary for the development of cell co...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269250
更新日期：1986-04-01 00:00:00
abstract：:Expiants of five plant species (Allium cepa, Antirrhinum majus, Brassica campestris. Glycine max, and Nicotiana tabacum) were co-cultivated with three Agrobacterium tumefaciens strains under different conditions to assess the effects of acetosyringone and medium pH on strain virulence. Tumours were incited on all dico...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00235354
更新日期：1991-04-01 00:00:00
abstract：KEY MESSAGE:The oil palm EgPAL1 gene promoter and its regulatory region were functional as a promoter in the heterologous system of Arabidopsis according to the cis-acting elements present in that region. The promoter was developmentally regulated, vascular tissue specific and responsive to water stress agents. Phenyla...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-017-2228-7
更新日期：2018-02-01 00:00:00
abstract：:A procedure to produce fruits from cultured shoot tips of Cucumis sativus L. cultivar 'Marketmore-76' in vitro is described. Four-week-old shoot tips, derived from sterile germinated seedlings on a MS medium, were cultured in a 3.8-1 Mason jar using an automated plant culture system. Tips readily generated roots, leav...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232312
更新日期：1993-11-01 00:00:00
abstract：: A system for barley transformation via polyethyleneglycol-mediated DNA uptake into protoplasts isolated directly from scutella and the regeneration of transgenic plants is reported. Scutellum protoplasts (cv. Clipper, an Australian malting cultivar) were co-transformed with plasmids Act 1-DGUS, containing the marker ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990000220
更新日期：2000-10-01 00:00:00
abstract：:Echinacea purpurea seedlings were inoculated with several Agrobacterium rhizogenes strains in order to obtain hairy roots. Infection with A. rhizogenes strains LMG63 and LMG150 resulted in callus formation. Upon infection with strains ATCC 15834 and R1601 hairy roots were obtained. Opine detection confirmed transforma...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00236463
更新日期：1991-06-01 00:00:00
abstract：KEY MESSAGE:We developed transgenic sweet potato with Spomin (sucrose-inducible minimal promoter)-GUS gene-fused constructs. Induced GUS activities by Spomin were higher than those by CaMV 35S promoter. We developed transgenic sweet potato (Ipomoea batatas L. Lam. cv. Kokei no. 14) plants with Spomin (sucrose-inducible...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-019-02453-7
更新日期：2019-11-01 00:00:00
abstract：:A procedure has been developed for isolating protoplasts from prothalli of Ceratopteris richardii which can be cultured and are capable of regeneration. Protoplasts were isolated from 2-week-old gametophytes in a medium containing wall-digesting enzymes in 0.5 M sucrose, followed by purification of the released protop...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050470
更新日期：1998-06-01 00:00:00
abstract：:Kanamycin-resistant plants of belladonna (Atropa belladonna) were obtained after Agrobacterium mediated transformation. When a rolC gene, which is one of the loci located on Ri plasmid of Agrobacterium rhizogenes, was co-introduced with a kanamycin resistant (NPT II) gene under control of a cauliflower mosaic virus 35...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232412
更新日期：1992-12-01 00:00:00
abstract：:Cell suspension cultures of Thalictrum minus L. var. hypoleucum Miq. were found to produce a large amount of berberine (400-800 mg/l) when 5-10 μM 6-benzyladenine was added to Linsmaier and Skoog's medium containing 100 μM 1-naphthaleneacetic acid. Most of the berberine produced was released continuously from the cell...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269306
更新日期：1984-12-01 00:00:00
abstract：:The effect ofin planta treatments with auxin inhibitors such as 2,3,5 triiodobenzoic acid (TIBA) on regeneration of plantsin vitro is not known. Here, we show the beneficial effect of preconditioning sugarbeet plants in the greenhouse with TIBA (3 mg/1) for efficientin vitro plant regeneration via a callus phase from ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF01890855
更新日期：1996-12-01 00:00:00
abstract：:Shoot cultures ofAtractylodes lancea DC. (Compositae) have been established by inoculating the flower bud on Linsmaier-Skoog's medium supplemented with 10(-5) M naphthaleneacetic acid and 10(-5) M 6-benzyl-aminopurine. Shoots were multiplied on a medium containing 10(-6) M 6-benzylaminopurine. Propagated shoots rooted...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF02441005
更新日期：1984-06-01 00:00:00
abstract：:Arabinogalactan proteins (AGPs) are important proteoglycans regulating somatic embryogenesis in diverse plant species. Embryogenic cells of somatic embryos are covered by special extracellular cell wall layer called extracellular surface matrix network (ECMSN) at their early developmental stages. Here we show that hig...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-007-0429-1
更新日期：2008-02-01 00:00:00
abstract：:Leaf mesophyll protoplasts isolated from pea (Pisum sativum L.) genotypes Century and PI244253 showed transient expression of β-glucuronidase (GUS) when electroporated with plasmid DNA containing various promoter-leader sequence constructs driving the GUS gene. The optimum conditions for transient expression were: usi...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232127
更新日期：1990-06-01 00:00:00
abstract：KEY MESSAGE:An ERF gene, LchERF , was cloned from L. chinense for the first time. Overexpression of LchERF conferred salt stress tolerance in transgenic tobacco lines during seed germination and vegetative growth. Ethylene-responsive transcription factors (ERFs) play important roles in tolerance to biotic and abiotic s...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-014-1678-4
更新日期：2014-12-01 00:00:00
abstract：KEY MESSAGE:This work provides a detailed histological analysis of the development of Jatropha curcas seeds, together with an assessment of the role of programmed cell death in this process. Seeds of Jatropha curcas are a potential source of raw material for the production of biodiesel, but very little is known about h...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-017-2184-2
更新日期：2017-11-01 00:00:00
abstract：:The activity and activation potential of polyphenoloxidase (PPO, E.C. 1.10.3.1.) of tissue from shoot tips, adult leaves and embryogenic and non-embryogenic cell suspension cultures of Euphorbia pulcherrima was investigated using an oxygen probe technique. PPO derived from differentiated in vivo plant tissue (shoot ti...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00234048
更新日期：1995-04-01 00:00:00
abstract：:The hybridity of eleven somatic hybrids between a diploidS. tuberosum and a diploidS. phureja clone could be verified because the parent karyotypes differed in their C-banding patterns. The hybrids were hypotetraploid and some carried structurally rearranged chromosomes and/or minute centric fragments. The nucleolar c...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00716844
更新日期：1989-02-01 00:00:00
abstract：:Callus derived from stem segments of loblolly pine (Pinus taeda L.) was subcultured to media containing 0.5% of various mono-, di-, tri- or polysaccharides. None of the carbohydrate sources tested were superior to sucrose. Growth on twelve of the 20 carbohydrates tested was more than 75% of that obtained for sucrose; ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00276676
更新日期：1987-04-01 00:00:00
abstract：:A genetic transformation protocol for green ash (Fraxinus pennsylvanica) hypocotyl explants was developed. Green ash hypocotyls were transformed using Agrobacterium tumefaciens strain EHA105 harboring binary vector pq35GR containing the neomycin phosphotransferase (nptII) and beta-glucuronidase (GUS) fusion gene, and ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-009-0697-z
更新日期：2009-06-01 00:00:00
abstract：:A novel genic male sterile (GMS) line in Brassica napus L., which was identified in 1999, was found to be controlled by a monogenic dominant gene, which we have designated as MDGMS. The microspores of the MDGMS abort before the degradation of the tapetal cell layer. The F1 fertility from any fertile lines crossed with...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-006-0276-5
更新日期：2007-05-01 00:00:00
