Release and crystallization of berberine in the liquid medium of Thalictrum minus cell suspension cultures.

Abstract:

:Cell suspension cultures of Thalictrum minus L. var. hypoleucum Miq. were found to produce a large amount of berberine (400-800 mg/l) when 5-10 μM 6-benzyladenine was added to Linsmaier and Skoog's medium containing 100 μM 1-naphthaleneacetic acid. Most of the berberine produced was released continuously from the cells into the liquid medium, and an excess of berberine crystallized as its nitrate in the medium. When the cells were cultured in a modified LS medium containing 20 mM KNO3 and 40 mM NH4Cl in place of 20.6 mM NH4NO3 as nitrogen source, most of the alkaloid crystallized to form berberine chloride instead of nitrate. Minor alkaloids, thalifendine and magnoflorine, were also isolated from the medium and identified.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Nakagawa K,Konagai A,Fukui H,Tabata M

doi

10.1007/BF00269306

subject

Has Abstract

pub_date

1984-12-01 00:00:00

pages

254-7

issue

6

eissn

0721-7714

issn

1432-203X

journal_volume

3

pub_type

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