Micropropagation of mature Chinese tallow tree (Sapium sebiferum Roxb.).

Abstract:

:An in vitro propagation technique based on axillary bud proliferation has been developed for matureSapium sebiferum trees. Nodal segments cultured on Murashige and Skoog (MS) medium supplemented with benzyl adenine (1-10 μM and α-naphthaleneacetic acid (0-0.5 μM showed axillary bud proliferation. Shoots proliferated in vitro were multiplied on Murashige and Skoog medium containing 2.5 μM benzyl adenine and 0.25 μM α-naphthaleneacetic acid. Seasonal changes affected the shoot proliferation potential of the initial explant. Shoots were rooted on a half-strength, growth-regulator-free, agar-gelled, MS medium after a 48-h treatment on half-strength MS liquid medium with 10 μM indole-3-butyric acid. Rooted plantlets were potted and acclimatized in a growth chamber and then moved to the greenhouse. Four-month-old plants were transplanted to the field.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Siril EA,Dhar U

doi

10.1007/BF01275506

subject

Has Abstract

pub_date

1997-06-01 00:00:00

pages

637-640

issue

9

eissn

0721-7714

issn

1432-203X

pii

10.1007/BF01275506

journal_volume

16

pub_type

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