Agrobacterium-mediated transformation of commercial melon (Cucumis melo L., cv. Amarillo Oro).

Abstract:

:Cotyledon explants of muskmelon (Cucumis melo L., cv. Amarillo Oro) seedlings were co-cultivated with disarmed Agrobacterium tumefaciens strain LBA4404 that contained the binary vector plasmid pBI121.1. The T-DNA region of this binary vector contains the Nopaline synthase/neomycin phosphotransferase II (NPTII) chimeric gene for kanamycin resistance and the Cauliflower Mosaic Virus 35S/β-glucuronidase (GUS) chimeric gene. After infection, the cotyledon pieces were placed in induction medium containing 100 mg/l kanamycin. Putative transformed shoots were obtained, followed by the development of morphologically normal plantlets. The transgenic nature of regenerants was demonstrated by polymerase chain reaction, Southern blot analysis, plant growth on medium selective for the transgene (NPTII) and expression of the co-transformed GUS gene. Factors affecting the transformation procedure are discussed.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Vallés MP,Lasa JM

doi

10.1007/BF00239881

subject

Has Abstract

pub_date

1994-01-01 00:00:00

pages

145-8

issue

3-4

eissn

0721-7714

issn

1432-203X

journal_volume

13

pub_type

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