Isolation and viral infection of Capsicum leaf protoplasts.

Abstract:

:A protocol for protoplast isolation was developed and tested with five Capsicum genotypes representing two cultivated species, C. annuum and C. chinense. Key variables included growth conditions for source plants and the concentration of mannitol used as osmoticum. Protoplasts isolated from each of the genotypes became infected when inoculated via electroporation with viral RNA from either pepper mottle potyvirus, tobacco etch potyvirus or cucumber mosaic cucumovirus.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Murphy JF,Kyle MM

doi

10.1007/BF00234146

subject

Has Abstract

pub_date

1994-04-01 00:00:00

pages

397-400

issue

7

eissn

0721-7714

issn

1432-203X

journal_volume

13

pub_type

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