Abstract:
:A protocol for protoplast isolation was developed and tested with five Capsicum genotypes representing two cultivated species, C. annuum and C. chinense. Key variables included growth conditions for source plants and the concentration of mannitol used as osmoticum. Protoplasts isolated from each of the genotypes became infected when inoculated via electroporation with viral RNA from either pepper mottle potyvirus, tobacco etch potyvirus or cucumber mosaic cucumovirus.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Murphy JF,Kyle MMdoi
10.1007/BF00234146subject
Has Abstractpub_date
1994-04-01 00:00:00pages
397-400issue
7eissn
0721-7714issn
1432-203Xjournal_volume
13pub_type
杂志文章abstract::Embryonal-suspensor tissue (EST) of Mediterranean cypress (Cupressus sempervirens L.) was tested for microprojectile-DNA delivery (by the PDS-1000/He device) for different subculture periods (9, 15, and 21 days) using the plasmid vectors pRT99GUS [containing the β-glucuronidase (GUS) and neomycin phosphotransferase (N...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050556
更新日期:1998-12-01 00:00:00
abstract::The non-yellowing gene (NYE1), initially identified from Arabidopsis, is a key regulatory gene responsible for chlorophyll degradation during senescence. Here, FaNYE1, an orthologue of AtNYE1, was further identified from a major type of cool-season turf grass, tall fescue (Festuca arundinacea Schreb.), by RACE-PCR. It...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1028-8
更新日期:2011-07-01 00:00:00
abstract::The lateral cotyledonary meristems of germinatingPisum sativum cv. Puget seeds were used to develop a reproducibleAgrobacterium tumefaciens-mediated transformation system. This procedure exhibits distinct advantages over those previously reported, in that it uses dry seed as starting material, and the highly regenerab...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF01142315
更新日期:1997-05-01 00:00:00
abstract:KEY MESSAGE:The dynamic alterations of the physiological and molecular processes in reproductive stage soybean indicated the dramatic impact caused by drought. Drought is a major abiotic stress that limits soybean (Glycine max) production. Most prior studies were focused on either model species or crops that are at the...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-018-2332-3
更新日期:2018-12-01 00:00:00
abstract:KEY MESSAGE:Transcriptome analysis of barley embryogenic callus from isolated microspore culture under salt stress uncovered a role of translation inhibition and selective activation of stress-specific proteins in cellular defense. Soil salinity is one of the major abiotic stresses which constrains the plant growth and...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-016-1986-y
更新日期:2016-08-01 00:00:00
abstract::Tillering is an important trait of cereal crops that optimizes plant architecture for maximum yield. Teosinte Branched 1 (TB1) is a negative regulator of lateral branching and an inducer of female inflorescence formation in Zea mays (maize). Recent studies indicate that TB1 homologs in Oryza sativa (rice), Sorghum bic...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1139-2
更新日期:2012-01-01 00:00:00
abstract::The ample supply of O2 proved to be of great importance for berberine production in cell suspension culture ofThalictrum minus, as the specific O2 consumption rate of berberine-producing cells was twice as high as that of non-producing cells. Furthermore, berberine yield increased with increases in the volumetric O2 t...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00778546
更新日期:1989-04-01 00:00:00
abstract::Somatic embryogenesis was induced in callus tissues derived from young flower buds ofPanax notoginseng via callus within 18 weeks of culture. The mature somatic embryos were germinated on half-strength Murashige and Skoog's (MS) medium supplemented with gibberellic acid A3(GA) and 6-benzyladenine (BA). The most suitab...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF01092764
更新日期:1997-04-01 00:00:00
abstract::The growth and sweet diterpene glucosides of Stevia plants propagated by stem-tip cultures were compared with those of the control plants propagated by seeds. There was no significant difference between the two groups both in growth and in chemical composition. As for the contents of sweet diterpene glucosides, howeve...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00270194
更新日期:1984-10-01 00:00:00
abstract::Eustoma grandiflorum Shinn requires vernalization for the induction of stem elongation and flowering. To investigate the role of gibberellins (GAs) in vernalization, the expression levels of genes encoding enzymes of GA biosynthesis, copalyl diphosphate synthetase, GA 20-oxidase and GA 3 beta-hydroxylase, were examine...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0672-z
更新日期:2003-09-01 00:00:00
abstract::Heading date in rice is an important agronomic trait controlled by several genes. In this study, flowering time of variety Dianjingyou 1 (DJY1) was earlier than a near-isogenic line (named NIL) carried chromosome segment from African rice on chromosome 3S, when grown in both long-day (LD) and short-day (SD) conditions...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1129-4
更新日期:2011-12-01 00:00:00
abstract:KEY MESSAGE:Transcript profiling during the early induction phase of oil palm tissue culture and RNAi studies in a model somatic embryogenesis system showed that EgENOD93 expression is essential for somatic embryogenesis. Micropropagation of oil palm through tissue culture is vital for the generation of superior and un...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-020-02571-7
更新日期:2020-11-01 00:00:00
abstract::The promotive effect of ethylene inhibitors (Els), i.e. AgNO3 and aminoethoxyvinylglycine (AVG) on de novo shoot regeneration from cultured cotyledonary explants of Brassica campestris ssp. pekinensis cv. Shantung in relation to polyamines (PAs) was investigated. The endogenous levels of free putrescine and spermidine...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232630
更新日期:1994-03-01 00:00:00
abstract::The first enzyme in the flavonoid pathway, chalcone synthase, is encoded by a gene (CHS) whose expression is normally under developmental control. In our previous studies, an 896-bp promoter region of a flower-specific CHS gene was isolated from Lilium orential 'Sorbonne', and designated as PLoCHS. Here, the PLoCHS pr...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1124-9
更新日期:2011-12-01 00:00:00
abstract::A procedure for inducing somatic embryos in shoot apex explants (2 mm) excised from shoot proliferation cultures established from adult oak trees (Quercus robur) was investigated. Embryogenesis was induced in shoot tip as well as leaf explants in three out of the five genotypes evaluated. Somatic embryos were formed b...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-010-0852-6
更新日期:2010-06-01 00:00:00
abstract::In the present paper, attempts were made to explore the possibility of employing ultraviolet (UV) irradiation in citrus asymmetric fusion for transfer of limited amount of favorable traits from a desirable cultivar to a target one. Exposure of Satsuma mandarin (Citrus unshiu Marc.) embryogenic protoplasts to UV at an ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-007-0350-7
更新日期:2007-08-01 00:00:00
abstract::Capsella bursa-pastoris (L.) Medic (2n = 4x = 32) is a natural double-low (erucic acid < 1%, glucosinolates < 30 micromol/g) germplasm and shows high degree of resistance to Sclerotinia sclerotiorum. Hybridizations were carried out between two Brassica species viz. B. rapa (2n = 20) and B. napus (2n = 38) as female an...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-007-0392-x
更新日期:2007-10-01 00:00:00
abstract::Carotenoid cleavage dioxygenases (CCDs) are involved in the production of diverse apocarotenoids including phytohormones, the visual molecules and the aromatic volatile compounds derived from carotenoids. Here, we examined the spatial expression of four of the CCD genes (AtCcd1, 4, 7 and 8) among the nine members of t...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-010-0999-1
更新日期:2011-06-01 00:00:00
abstract::Mimosa tenuiflora (Willd.) Poiret (Leguminosae) was micropropagated throughin vitro culture of axillary buds on Murashige and Skoog (MS) medium. Shoot formation was achieved when the media were supplemented with 0.1 mg.L(-1) IAA + 3 mg.L(-1) KN.In vitro rooting of regenerated shoots was achieved when 0.1 mg.L(-1) KN w...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF01275455
更新日期:1996-11-01 00:00:00
abstract::DNA fingerprints generated by the Jeffreys' probes, 33.6 and 33.15, indicated the presence of minisatellite-like sequences in the red clover genome. The fingerprints generated by probe 33.6 gave less background and fewer but better defined bands than those obtained with probe 33.15. Assay of a regenerative somaclonal ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00235293
更新日期:1993-12-01 00:00:00
abstract::Plating rice anthers on a semisolid induction medium containing 250 or 500 mg/l colchicine for 24 or 48 h-incubations followed by transfer to colchicine-free medium and standard anther culture procedures resulted in overall 1.5- to 2.5- fold increases in doubled haploid green plant productions compared to control anth...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00231961
更新日期:1994-05-01 00:00:00
abstract::Axillary buds from 5 genotypes of mulberry belonging to 4 species were cultured on modified MS basal medium. A total of 30 media combinations were tried for all the genotypes. The response of axillary buds and the requirement for growth regulators varied with genotype. In Morus indica BAP (0.25-0.5 mg/l), and in M. al...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00272107
更新日期:1990-05-01 00:00:00
abstract::A differential expression study on the Saccharum spp.-Sporisorium scitamineum pathogenic interaction was carried out in the susceptible Ja60-5 and the resistant M31/45 genotypes. Using cDNA-AFLP analysis, a total of 64 transcript-derived fragments (TDFs) was found to be differentially expressed, with the majority (67....
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-008-0524-y
更新日期:2008-06-01 00:00:00
abstract:KEY MESSAGE:In GhCFE5 homoeologs, GhCFE5D interacted with more actin homologs and stronger interaction activity than GhCFE5A. GhCFE5D - but not GhCFE5A -overexpression severely disrupted actin cytoskeleton organization and significantly suppressed cell elongation. Homoeologous genes are common in polyploid plants; howe...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-015-1928-0
更新日期:2016-04-01 00:00:00
abstract::Haploid plants were regenerated from cultured unfertilized ovaries of Hordeum vulgare L. (barley). Optimal response was obtained by the addition of 0.6 μM 4-chloro-2-methylphenoxyacetic acid (MCPA), 2.8 μM indole-3-acetic acid (IAA) and 4.4 μM 6-benzyladenine (BA) in the N6 medium. Further increase in the rate of call...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00239094
更新日期:1993-01-01 00:00:00
abstract::Plant regeneration in Arachis pintoi was obtained via two developmental pathways: organogenesis and somatic embryogenesis. Organogenic callus cultures were initiated from pieces of leaf on MS medium supplemented with NAA or 2,4-D in combination with BA, KIN or 2iP. The most suitable combination for plant regeneration ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990000198
更新日期:2000-09-01 00:00:00
abstract:: Somatic embryogenesis and whole plant regeneration were achieved in callus cultures derived from immature zygotic embryos of Acacia mangium. Embryogenic callus was induced on MS medium containing combinations of TDZ (1-2 mg/l), IAA (0.25-2 mg/l) and a mixture of amino acids. Globular embryos developed on embryogenic ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990000288
更新日期:2001-01-01 00:00:00
abstract::Callus cultures from cotyledon explants were established and maintained in culture for more than two years. After several months callus cultures were transferred into liquid medium and cultured as cell suspensions. Protoplasts were isolated from these cell suspension cultures and cultured in a liquid medium. After for...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269184
更新日期:1982-06-01 00:00:00
abstract::The ability to germinate orchids from seeds in vitro presents a useful and viable method for the propagation of valuable germplasm, maintaining the genetic heterogeneity inherent in seeds. Given the ornamental and medicinal importance of many species within the genus Dendrobium, this review explores in vitro technique...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-015-1829-2
更新日期:2015-10-01 00:00:00
abstract::A protocol for Agrobacterium-mediated transformation with mannose selection was developed for cotyledon petiole, hypocotyl and leaf explants of tomato (Lycopersicon esculentum L. Mill). More than 400 transgenic plants from three tomato varieties were selected with 1% mannose in combination with 0.1-0.5% glucose. Avera...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-004-0809-8
更新日期:2004-10-01 00:00:00