Vascular-specific activity of the Arabidopsis carotenoid cleavage dioxygenase 7 gene promoter.

Abstract:

:Carotenoid cleavage dioxygenases (CCDs) are involved in the production of diverse apocarotenoids including phytohormones, the visual molecules and the aromatic volatile compounds derived from carotenoids. Here, we examined the spatial expression of four of the CCD genes (AtCcd1, 4, 7 and 8) among the nine members of this family in Arabidopsis by RT-PCR. We found that the AtCcd7 gene showed strong expression in seeds. However, the promoter activity of the 1,867-bp 5'-upstream region of this gene exhibited a vascular specificity at all developmental stages throughout the transgenic Arabidopsis plants tested. The strength of the AtCcd7 promoter was also found to be lower than that of the 35S promoter by about 60%. The whole body expression of the β-glucuronidase (GUS) reporter gene driven by the AtCcd7 promoter in Arabidopsis plants was confirmed in different organs by RT-PCR and GUS enzymatic assays. Histochemical GUS staining further revealed that the AtCcd7 promoter has utility in limiting the expression of target genes to the vascular tissues in all plant organs such as the leaf, stem, root, flower and seed.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Liang YS,Jeon YA,Lim SH,Kim JK,Lee JY,Kim YM,Lee YH,Ha SH

doi

10.1007/s00299-010-0999-1

subject

Has Abstract

pub_date

2011-06-01 00:00:00

pages

973-80

issue

6

eissn

0721-7714

issn

1432-203X

journal_volume

30

pub_type

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