Abstract：
KEY MESSAGE:Oral administration of maize-expressed H3N2 nucleoprotein induced antibody responses in mice showing the immunogenicity of plant-derived antigen and its potential to be utilized as a universal flu vaccine. Influenza A viruses cause influenza epidemics that are devastating to humans and livestock. The vaccine for influenza needs to be reformulated every year to match the circulating strains due to virus mutation. Influenza virus nucleoprotein (NP) is a multifunctional RNA-binding protein that is highly conserved among strains, making it a potential candidate for a universal vaccine. In this study, the NP gene of H3N2 swine origin influenza virus was expressed in maize endosperm. Twelve transgenic maize lines were generated and analyzed for recombinant NP (rNP) expression. Transcript analysis showed the main accumulation of rNP in seed. Protein level of rNP in T1 transgenic maize seeds ranged from 8.0 to 35 µg of NP/g of corn seed. The level increased up to 70 µg of NP/g in T3 seeds. A mouse study was performed to test the immunogenicity of one line of maize-derived rNP (MNP). Mice were immunized with MNP in a prime-boost design. Oral gavage administration showed that a humoral immune response was elicited in the mice treated with MNP indicating the immunogenicity of MNP. NP-specific antibody responses in the MNP group showed comparable antibody titer with the groups receiving positive controls such as Vero cell-derived NP (VNP) or alphavirus replicon particle-derived NP (ANP). Cytokine analysis showed antigen-specific stimulation of IL-4 cytokine elicited in splenocytes from mice treated with MNP further confirming a TH2 humoral immune response induced by MNP administration.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Nahampun HN,Bosworth B,Cunnick J,Mogler M,Wang Kdoi
10.1007/s00299-015-1758-0subject
Has Abstractpub_date
2015-06-01 00:00:00pages
969-80issue
6eissn
0721-7714issn
1432-203Xjournal_volume
34pub_type
杂志文章abstract：:Treatment of Allium cepa L. cellsuspension cultures with a biotic elicitor derived from the fungus Botrytis cinerea, resulted in phytoalexin synthesis. Two phytoalexins, 5-octylcyclopenta-1,3-dione and 5-hexyl-cyclopenta-1,3-dione, were accumulated in cultured onion cells. Removal of extracellular Ca(2+) by the calciu...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00231593
更新日期：1996-09-01 00:00:00
abstract：:Molecular analysis of gene expression differences between green and red lettuce leaves was performed using the SSH method. BlastX comparisons of subtractive expressed sequence tags (ESTs) indicated that 7.6% of clones encoded enzymes involved in secondary metabolism. Such clones had a particularly high abundance of fl...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-006-0255-x
更新日期：2007-04-01 00:00:00
abstract：:The Golgi apparatus in plants is organized as a multitude of individual stacks that are motile in the cytoplasm and in close association with the endoplasmic reticulum (ER) (Boevink et al. in Plant J 15:441-447, 1998). These stacks operate as a sorting centre for cargo molecules, providing modification and redirection...
journal_title：Plant cell reports
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doi：10.1007/s00299-007-0390-z
更新日期：2007-09-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-007-0429-1
更新日期：2008-02-01 00:00:00
abstract：:Cell suspension cultures of Nicotiana were initiated using conditions designed to selectively favor stable chromosome number. These conditions included use of leaf explants to initiate cultures, growth of cells in culture medium containing 2,4-D, and transfer of cells with short subculture intervals. Four cell lines d...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00272364
更新日期：1982-04-01 00:00:00
abstract：:A protocol was developed for biolistic transformation of hybrid bermudagrass cv. TifEagle using the bar gene. TifEagle is an ultradwarf used exclusively on golf greens. Herbicide resistance should serve as a useful management tool, especially if methyl-bromide is unavailable for fumigation prior to plant establishment...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-003-0736-0
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00237431
更新日期：1993-04-01 00:00:00
abstract：KEY MESSAGE:Transcript profiling during the early induction phase of oil palm tissue culture and RNAi studies in a model somatic embryogenesis system showed that EgENOD93 expression is essential for somatic embryogenesis. Micropropagation of oil palm through tissue culture is vital for the generation of superior and un...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-020-02571-7
更新日期：2020-11-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-011-1045-7
更新日期：2011-07-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00270112
更新日期：1988-03-01 00:00:00
abstract：:A procedure to produce fruits from cultured shoot tips of Cucumis sativus L. cultivar 'Marketmore-76' in vitro is described. Four-week-old shoot tips, derived from sterile germinated seedlings on a MS medium, were cultured in a 3.8-1 Mason jar using an automated plant culture system. Tips readily generated roots, leav...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232312
更新日期：1993-11-01 00:00:00
abstract：KEY MESSAGE:Effective microspore embryogenesis in triticale is determined by a specific hormonal homeostasis: low value of IAA/cytokinins, IAA/ABA and cytokinins/ABA ratios as well as proper endogenous/exogenous auxin balance, which favours androgenic structure formation and green plant regeneration ability. The concen...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-014-1686-4
更新日期：2015-01-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-008-0563-4
更新日期：2008-08-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00272059
更新日期：1989-03-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-007-0413-9
更新日期：2007-11-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-012-1320-2
更新日期：2012-11-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269240
更新日期：1986-04-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-010-0835-7
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-007-0321-z
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00716668
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journal_title：Plant cell reports
pub_type： 杂志文章
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更新日期：2000-05-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF02441015
更新日期：1984-06-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-008-0539-4
更新日期：2008-07-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF01275445
更新日期：1996-11-01 00:00:00
abstract：:It has been reported that long term cell cultures from maize endosperms are not completely de-differentiated, maintaining some tissue-specific synthesis. We analyzed the expression of zein (the major storage protein of maize seeds) in cultures derived from wildtype and opaque-2 maize endosperms. In wildtype cultures, ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00272049
更新日期：1989-03-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00233096
更新日期：1992-10-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00231583
更新日期：1996-09-01 00:00:00
abstract：:We describe a simple and efficient protocol for regeneration-transformation of two diploid Medicago lines: the annual M. truncatula R108-1(c3) and the perennial M. sativa ssp. falcata (L.) Arcangeli PI.564263 selected previously as highly embryogenic genotypes. Here, embryo regeneration of R108-1 to complete plants wa...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050405
更新日期：1998-03-01 00:00:00
abstract：:RNA interference (RNAi) is a sequence-specific, posttranscriptional gene silencing (PTGS) process in plants that is mediated by dsRNA homologous to the silenced gene(s). In this study, we report an efficient method to produce dsRNA using a bacterial expression system. Two fragments of the Sugarcane Mosaic Virus (SCMV)...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-010-0911-z
更新日期：2010-11-01 00:00:00
