Production of gynogenic haploids of Hordeum vulgare L.

Abstract:

:Haploid plants were regenerated from cultured unfertilized ovaries of Hordeum vulgare L. (barley). Optimal response was obtained by the addition of 0.6 μM 4-chloro-2-methylphenoxyacetic acid (MCPA), 2.8 μM indole-3-acetic acid (IAA) and 4.4 μM 6-benzyladenine (BA) in the N6 medium. Further increase in the rate of callus formation and the number of green plants produced was possible with the addition of 90 g/l sucrose and 100 g/l coconut water. The stage of development of the ovaries at the time of culture was critical; the largest number of plants being produced by ovaries from flowers at the trinucleate stage of pollen.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Castillo AM,Cistué L

doi

10.1007/BF00239094

subject

Has Abstract

pub_date

1993-01-01 00:00:00

pages

139-43

issue

3

eissn

0721-7714

issn

1432-203X

journal_volume

12

pub_type

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