Abstract:
:Haploid plants were regenerated from cultured unfertilized ovaries of Hordeum vulgare L. (barley). Optimal response was obtained by the addition of 0.6 μM 4-chloro-2-methylphenoxyacetic acid (MCPA), 2.8 μM indole-3-acetic acid (IAA) and 4.4 μM 6-benzyladenine (BA) in the N6 medium. Further increase in the rate of callus formation and the number of green plants produced was possible with the addition of 90 g/l sucrose and 100 g/l coconut water. The stage of development of the ovaries at the time of culture was critical; the largest number of plants being produced by ovaries from flowers at the trinucleate stage of pollen.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Castillo AM,Cistué Ldoi
10.1007/BF00239094subject
Has Abstractpub_date
1993-01-01 00:00:00pages
139-43issue
3eissn
0721-7714issn
1432-203Xjournal_volume
12pub_type
杂志文章abstract::A microscopical study was performed in order to localize chromogenic valepotriates in roots (in vivo) and in callus and root organ cultures (in vitro) of valerian plants. These in vitro cultures (producing valepotriates) contained lipid droplets having the same aspect as those containing essential oil described in the...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00270186
更新日期:1983-12-01 00:00:00
abstract::Conditions favourable to embryogenesis from isolated microspores of Brassica rapa L. ssp. oleifera (canola quality) were identified. A population with enhanced responsiveness for microspore embryogenesis (C200) was synthesized by crossing individual plants showing microspore embryogenic potential. For optimal microspo...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232537
更新日期:1992-05-01 00:00:00
abstract::Plants have their own mechanisms for overcoming various stresses. In cold regions, plants are subject to stress and must enter an inherent dormancy, through several complex mechanisms, if they are to continue to exist. In winter, regulation of tonoplast and plasma membrane aquaporin genes differed in the bud cushions ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-009-0770-7
更新日期:2009-11-01 00:00:00
abstract::Five varieties of durum wheat: Appulo, Ofanto, Latino, Creso, and Castello (Triticum durum Desf.) adapted to the semi-arid mediterranean environment have been tested for their in vitro response. Compact, embryogenic, highly regenerable calli originated from primary callus derived through proliferation of the scutellum...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00193145
更新日期:1991-09-01 00:00:00
abstract::The ability to germinate orchids from seeds in vitro presents a useful and viable method for the propagation of valuable germplasm, maintaining the genetic heterogeneity inherent in seeds. Given the ornamental and medicinal importance of many species within the genus Dendrobium, this review explores in vitro technique...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-015-1829-2
更新日期:2015-10-01 00:00:00
abstract::Previous work has shown that in rice suspension cells, NaCl at 0.4 M can induce Em gene expression and act synergistically with ABA, possibly by potentiating the ABA response pathway through a rate-limiting intermediate (R.M. Bostock and R.S. Quatrano (1992) Plant Physiol., 98, 1356-1363). Since calcium is an intermed...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232374
更新日期:1996-01-01 00:00:00
abstract::Cultured cells of tobacco (Nicotiana tabacum L. cv Petit Havana) were used to investigate signals regulating the expression of the "model" nuclear gene encoding the alternative oxidase (AOX) (AOX1), the terminal oxidase of the mitochondrial alternative respiratory pathway. Several conditions shown to induce AOX1 mRNA ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-004-0848-1
更新日期:2004-12-01 00:00:00
abstract::A cDNA clone encoding an ADP-ribosylation factor from potato (Solanum tuberosum L.) was isolated. The nucleotide and deduced amino acid sequences show high homology to known ADP-ribosylation factor sequences from Arabidopsis, yeast, cow and man. In northern blot experiments, all tissues analysed showed expression of t...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233786
更新日期:1994-12-01 00:00:00
abstract::Site-specific recombination has been developed into a genetic engineering tool for higher eukaryotes. The manipulation of newly introduced DNA is now possible in the course of genetic transformation procedures, thus making the process more predictable and reliable. Also, a wide variety of chromosomal rearrangements us...
journal_title:Plant cell reports
pub_type: 杂志文章,评审
doi:10.1007/s00299-003-0616-7
更新日期:2003-06-01 00:00:00
abstract:KEY MESSAGE:Transcription factors from mammals and plants, which play a role in innate immunity, interact with the same microbe-associated molecular pattern (MAMP)-responsive sequences from Arabidopsis thaliana. The interaction of mouse NF-κB p65 with MAMP-responsive sequences containing the core motif GACTTT of the WT...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-019-02433-x
更新日期:2019-09-01 00:00:00
abstract::We report here the successful establishment of callus, cell and root cultures from explants of in-vitro-grown plantlets of the soapwort Saponaria officinalis L. The production of saporin in the different tissue systems was evaluated by determining the capability of crude extracts to inactivate protein synthesis and by...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050351
更新日期:1997-11-01 00:00:00
abstract::The capability of Phalaenopsis to acclimate its photosynthetic capacity and metabolic activity to cool night temperature conditions is crucial for improving orchid production in terms of efficient greenhouse heating. The extent to which Phalaenopsis possesses acclimation potential and the mechanistic background of the...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1021-2
更新日期:2011-06-01 00:00:00
abstract::Quantitative trait loci (QTLs) associated with androgenic responsiveness in triticale were analyzed using a population of 90 DH lines derived from the F1 cross between inbred line 'Saka 3006' and cv. 'Modus', which was used in a number of earlier studies on molecular mapping in this crop. Using Windows QTL Cartographe...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-012-1320-2
更新日期:2012-11-01 00:00:00
abstract::Processes involved in transformation of regenerable soybean (Glycine max (L.) Merrill) immature zygotic cotyledons were studied by assaying the transient expression of the β-glucuronidase gene driven by the 35S promoter and terminated at the 3' end by the soybean 7S storage protein gene. The plasmid containing the chi...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00234510
更新日期:1994-07-01 00:00:00
abstract::The AtGRP5 gene from Arabidopsis thaliana encodes a glycine-rich protein which has a major activity in protoderm-derived cells and is expressed in cells that undergo the first anatomical modifications leading to somatic embryo development. It has been previously demonstrated that its minimum promoter is 316 bp long in...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-010-0835-7
更新日期:2010-05-01 00:00:00
abstract::Expression of the green fluorescent protein (gfp) gene, under regulatory control of either the constitutive 35S promoter or the developmentally-regulated lectin promoter was monitored and quantified using a newly-developed automated tracking system. The automated system consisted of a computer-controlled two-dimension...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-006-0142-5
更新日期:2006-09-01 00:00:00
abstract::TransgenicRhododendron plants were obtained byAgrobacterium tumefaciens-mediated gene transfer.A. tumefaciens harboring a binary vector that contained the chimeric neomycin phosphotransferase II (NPTII) and (3-glucuronidase (GUS) genes was co-cultivated with stem and leaf segments fromRhododendron tissues culturedin v...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF01275445
更新日期:1996-11-01 00:00:00
abstract::In this paper we describe a simple method to improve the plating efficiency in plant cell cultures.Two-stage plating is used; in the first stage the cells are inoculated at high density in 0.2% agarized culture medium for ten days to facilitate growth; under this condition, each cell produces a single micro-colony tra...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00571305
更新日期:1985-05-01 00:00:00
abstract::The aim of this study was the improvement of embryo production in wheat anther culture. Three butanol alcohols, n-butanol, sec-butanol and tert-butanol, were evaluated for their effect on microspore embryogenesis in two spring cultivars of wheat, Pavon and Caramba. Application of n-butanol, at 0.1 and 0.2% (v/v) in th...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-007-0500-y
更新日期:2008-05-01 00:00:00
abstract::The variation among and within different populations of the regeneration ability from leaf, cotyledon and hypocotyl explants has been studied. A control population and two lines selected by their regeneration capacity from leaf explants were used. Significant differences among the plants of the control population,for ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF01690269
更新日期:1995-01-01 00:00:00
abstract::Tissue culture methods for improvement of cotton has lagged seriously compared to other major crops. A method for regeneration of cotton which includes a morphogenetically competent cell suspension was needed to facilitate selection of stress-resistant variants and gene manipulation. Preliminary screening of eight str...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00268487
更新日期:1987-06-01 00:00:00
abstract::The interconversion between 5 to 8-membered cycloalkanones and the corresponding cycloalkanols by a cell suspension culture of Nicotiana tabacum was studied. It was found that the interconversion is at equilibrium and the balance in the equilibrium depends on the number of carbon atoms in the carbocyclic ring of the s...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00270167
更新日期:1983-04-01 00:00:00
abstract::Eustoma grandiflorum Shinn requires vernalization for the induction of stem elongation and flowering. To investigate the role of gibberellins (GAs) in vernalization, the expression levels of genes encoding enzymes of GA biosynthesis, copalyl diphosphate synthetase, GA 20-oxidase and GA 3 beta-hydroxylase, were examine...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0672-z
更新日期:2003-09-01 00:00:00
abstract::Human growth hormone (hGH), a pituitary-derived polypeptide, evidences a wide range of biological functions, including protein synthesis, cell proliferation, and metabolism. A synthetic hGH gene (shGH) has been synthesized on the basis of plant-optimized codon usage via an overlap PCR strategy and located in a plant e...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-008-0514-0
更新日期:2008-05-01 00:00:00
abstract:KEY MESSAGE:Two translation-related proteins are identified as FMT-interacting proteins. However, FMT, unlike mutants of other CLU genes in fly and human, has no clear impact on the accumulation of mitochondrial proteins. Organelle distribution is critical for effective metabolism and stress response and is controlled ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-020-02634-9
更新日期:2021-02-01 00:00:00
abstract::KEY MESSAGE : Two soybean cultivars showed markedly different drought tolerance. G6PDH plays a central role in the process of H ( 2 ) O ( 2 ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-012-1374-1
更新日期:2013-03-01 00:00:00
abstract::Ethylene emanation rates were assessed from leaf tissues of an embryogenic seed plant (Cycle 0) and regeneration cycle plants selected for enhanced embryogenesis (Cycles I, II and IV). In all experiments, ethylene was assessed from the basal 1 cm portion of the innermost leaf. Ethylene emanation was five-fold higher i...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00272059
更新日期:1989-03-01 00:00:00
abstract:KEY MESSAGE:Several hypomethylated sites within the Karma region of EgDEF1 and hotspot regions in chromosomes 1, 2, 3, and 5 may be associated with mantling. One of the main challenges faced by the oil palm industry is fruit abnormalities, such as the "mantled" phenotype that can lead to reduced yields. This clonal abn...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-020-02561-9
更新日期:2020-09-01 00:00:00
abstract::Protolasts of Bupleurum scorzonerifolium were prepared from stem node-derived embryogenic calli with an enzyeme mixture, in which snailase was a necessary component. Follolwing cell wall regeneration protoplasts divided and directly formed somatic embryos which developed into plantlets. The conditions favorable to dir...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232170
更新日期:1992-04-01 00:00:00
abstract::The steroidal hormone brassinosteroids (BRs) play important roles in plant growth and development. Genetic, genomic and proteomic studies in Arabidopsis have identified major BR signaling components and elucidated the signal transduction pathway from the cell surface receptor kinase BRI1 to the BES1/BZR1 family of tra...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-013-1438-x
更新日期:2013-07-01 00:00:00