Asymbiotic in vitro seed propagation of Dendrobium.

Abstract:

:The ability to germinate orchids from seeds in vitro presents a useful and viable method for the propagation of valuable germplasm, maintaining the genetic heterogeneity inherent in seeds. Given the ornamental and medicinal importance of many species within the genus Dendrobium, this review explores in vitro techniques for their asymbiotic seed germination. The influence of abiotic factors (such as temperature and light), methods of sterilization, composition of basal media, and supplementation with organic additives and plant growth regulators are discussed in context to achieve successful seed germination, protocorm formation, and further seedling growth and development. This review provides both a basis for the selection of optimal conditions, and a platform for the discovery of better ones, that would allow the development of new protocols and the exploration of new hypotheses for germination and conservation of Dendrobium seeds and seedlings.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Teixeira da Silva JA,Tsavkelova EA,Ng TB,Parthibhan S,Dobránszki J,Cardoso JC,Rao MV,Zeng S

doi

10.1007/s00299-015-1829-2

subject

Has Abstract

pub_date

2015-10-01 00:00:00

pages

1685-706

issue

10

eissn

0721-7714

issn

1432-203X

pii

10.1007/s00299-015-1829-2

journal_volume

34

pub_type

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