Production of flavanone-neohesperidosides in Citrus embryos.

abstract：:Green plants were repeatedly regenerated from suspension-derived protoplasts of Kentucky blue grass (Poa pratensis L.) cv. "Geronimo". One suspension was capable of donating competent protoplasts during long-term culture i. e. 10-16 months after its establishment. The plating efficiency of the protoplasts from three d...

journal_title：Plant cell reports

authors： Nielsen KA,Larsen E,Knudsen E

更新日期：1993-08-01 00:00:00

abstract：KEY MESSAGE:The T.118 and T.406 seedlings showed strong adaptability under Cd concentrations ≤ 50 µM. The mechanisms of photoprotection in T.118 and T.406 differed in high-Cd concentrations. To explore the physiological response characteristics of Taxodium hybrids to cadmium (Cd) stress and provide basis for screening ...

journal_title：Plant cell reports

authors： Ma YL,Wang HF,Wang P,Yu CG,Luo SQ,Zhang YF,Xie YF

更新日期：2018-11-01 00:00:00

abstract：:The ample supply of O2 proved to be of great importance for berberine production in cell suspension culture ofThalictrum minus, as the specific O2 consumption rate of berberine-producing cells was twice as high as that of non-producing cells. Furthermore, berberine yield increased with increases in the volumetric O2 t...

journal_title：Plant cell reports

authors： Kobayashi Y,Fukui H,Tabata M

更新日期：1989-04-01 00:00:00

abstract：: This study was conducted to detect the optimum conditions for DNA transfer into mature embryos of barley via electroporation. Cultured mature embryos of barley were directly electroporated in the presence of the pBI 121 vector carrying both the β-glucuronidase (GUS) and neomycin phosphotransferase II (npt II) genes. ...

journal_title：Plant cell reports

authors： Gürel F,Gözükirmizi N

更新日期：2000-07-01 00:00:00

abstract：KEY MESSAGE:A plant-derived 0.3 kb constitutive promoter was obtained from AtTCTP expression analysis, and successfully applied to the expression of a selectable marker gene for production of transgenic creeping bentgrass plants. The isolation and use of an efficient promoter is essential to develop a vector system for...

journal_title：Plant cell reports

authors： Han YJ,Kim YM,Hwang OJ,Kim JI

更新日期：2015-02-01 00:00:00

abstract：:Cryopreservation has been shown to improve the frequency of virus elimination - specifically cucumber mosaic virus and banana streak virus - from banana ( Musa spp.) plants. To understand the mode of action of cryopreservation for the eradication of viral particles, we examined the ultrastructure of meristem tips at e...

journal_title：Plant cell reports

authors： Helliot B,Swennen R,Poumay Y,Frison E,Lepoivre P,Panis B

更新日期：2003-03-01 00:00:00

abstract：:In the coenobia-forming green alga, Hydrodictyon reticulatum, changes in DNA composition during growth and differentiation of reproductive cells were followed. A "loss" of DNA per nucleus could be attributed to underreplication of an amplified DNA fraction being part of a (GC)-rich satellite band in CsCl gradients of ...

journal_title：Plant cell reports

authors： Ssymank V

更新日期：1983-10-01 00:00:00

abstract：:The first enzyme in the flavonoid pathway, chalcone synthase, is encoded by a gene (CHS) whose expression is normally under developmental control. In our previous studies, an 896-bp promoter region of a flower-specific CHS gene was isolated from Lilium orential 'Sorbonne', and designated as PLoCHS. Here, the PLoCHS pr...

journal_title：Plant cell reports

authors： Liu Y,Lou Q,Xu W,Xin Y,Bassett C,Wang Y

更新日期：2011-12-01 00:00:00

abstract：: Cells of a suspension culture of Doritaenopsis cv. New Toyohashi were placed in a mixture of 2 M glycerol and 0.4 M sucrose for 15 min at room temperature and then dehydrated with a vitrification solution (PVS2) for 1-3 h on ice and plunged into liquid nitrogen. The highest viability (64% by 2,3,5-triphenyltetrazoliu...

journal_title：Plant cell reports

authors： Tsukazaki H,Mii M,Tokuhara K,Ishikawa K

更新日期：2000-12-01 00:00:00

abstract：:We have assessed the capacity of cultured protoplasts from two tissue sources of several commercially-grown broccoli cultivars to regenerate plants. A procedure that employs hypocotyl protoplasts and a culture medium with a high NAA:2,4-D auxin ratio was developed. The procedure permits highly efficient formation of c...

journal_title：Plant cell reports

authors： Kao HM,Keller WA,Gleddie S,Brown GG

更新日期：1990-10-01 00:00:00

abstract：:Extracts of cranberry, rich in flavonols and proanthocyanins, inactivated β-glucuronidase (GUS) in assays using either purified bacterial GUS or preparations of transgenic tobacco (Nicotiana tabacum L.) or transgenic cranberry (Vaccinium macrocarpon Ait.) expressing the gusA gene. Histochemical GUS assays produced ran...

journal_title：Plant cell reports

authors： Serres R,McCown B,Zeldin E

更新日期：1997-06-01 00:00:00

abstract：:An Arabidopsis mutant line T90, exhibiting a stem-specific and wound-responsive GUS expression was identified from a population of Arabidopsis thaliana tagged with a promoterless β-glucuronidase (GUS) in the T-DNA. Sequence flanking the insertion from the right border was amplified by TAIL PCR and cloned. The insertio...

journal_title：Plant cell reports

authors： Gupta NC,Jain PK,Bhat SR,Srinivasan R

更新日期：2012-05-01 00:00:00

abstract：:While searching for genes expressed in acid lemon but not in acidless lime pulp, we isolated clone Cl111 which showed the following expression phenotypes: (1) while it was expressed in the ovaries in both varieties, its mRNA was detected only in the pulp of the acid fruit, (2) no or very low expression of the gene was...

journal_title：Plant cell reports

authors： Sorkina A,Bardosh G,Liu YZ,Fridman I,Schlizerman L,Zur N,Or E,Goldschmidt EE,Blumwald E,Sadka A

更新日期：2011-09-01 00:00:00

abstract：:The effect of various inhibitors on differentiation (shoot morphogenesis) in callus cultures of Brassica, Datura and Nicotiana has been investigated. Hormone medium without any inhibitor (control), resulted in 6% shoot formation. Addition of inhibitors such as actinomycin D, cordycepin, abscisic acid, trigonelline and...

journal_title：Plant cell reports

authors： Sethi U,Basu A,Mukherjee SG

更新日期：1990-03-01 00:00:00

abstract：:Expression of the green fluorescent protein (gfp) gene, under regulatory control of either the constitutive 35S promoter or the developmentally-regulated lectin promoter was monitored and quantified using a newly-developed automated tracking system. The automated system consisted of a computer-controlled two-dimension...

journal_title：Plant cell reports

authors： Buenrostro-Nava MT,Ling PP,Finer JJ

更新日期：2006-09-01 00:00:00

abstract：:A cDNA clone encoding an ADP-ribosylation factor from potato (Solanum tuberosum L.) was isolated. The nucleotide and deduced amino acid sequences show high homology to known ADP-ribosylation factor sequences from Arabidopsis, yeast, cow and man. In northern blot experiments, all tissues analysed showed expression of t...

journal_title：Plant cell reports

authors： Szopa J,Müller-Röber B

更新日期：1994-12-01 00:00:00

abstract：:A procedure has been developed for the clonal propagation of Picrorhiza kurroa Royle ex Benth. through shoot tip culture. Murashige and Skoog's medium (1962) supplemented with kinetin (3.0 to 5.0 mg/l) supported rapid proliferation of multiple shoots from the explants. Addition of indole-3-acetic acid (1.0 mg/l) to th...

journal_title：Plant cell reports

authors： Lal N,Ahuja PS,Kukreja AK,Pandey B

更新日期：1988-05-01 00:00:00

abstract：:In a study of the 5'-flanking sequence of the Zea mays L. (maize) Glb1 gene in vitro, serial promoter deletions were generated and linked with the β-glucuronidase (GUS) reporter gene. The promoter deletion-GUS fusions were introduced into the maize P3377 cell line by particle bombardment. GUS assays indicated that tre...

journal_title：Plant cell reports

authors： Liu S,Kriz A,Duncan D,Widholm J

更新日期：1998-05-01 00:00:00

abstract：KEY MESSAGE:Functional characterization of two tobacco genes, one involved in xylan synthesis and the other, a positive regulator of secondary cell wall formation, is reported. Lignocellulosic secondary cell walls (SCW) provide essential plant materials for the production of second-generation bioethanol. Therefore, tho...

journal_title：Plant cell reports

authors： Pandey SK,Nookaraju A,Fujino T,Pattathil S,Joshi CP

更新日期：2016-11-01 00:00:00

abstract：:Four diverse strains of Agrobacterium tumefaciens (C58, Ach5, GV3111, and A281) were capable of inducing tumors at a high frequency on inoculated stems of lentil (Lens culinaris Medik. cultivar Laird) in vivo, and on excised shoot apices in vitro. GV3111 and Ach5 produced the largest and heaviest tumors in vivo, while...

journal_title：Plant cell reports

authors： Warkentin TD,McHughen A

更新日期：1991-12-01 00:00:00

abstract：KEY MESSAGE:An RNAseq-based analysis of the cassava plants inoculated with Xam allowed the identification of transcriptional upregulation of genes involved in jasmonate metabolism, phenylpropanoid biosynthesis and putative targets for a TALE. Cassava bacterial blight, a disease caused by the gram-negative bacterium Xan...

journal_title：Plant cell reports

authors： Muñoz-Bodnar A,Perez-Quintero AL,Gomez-Cano F,Gil J,Michelmore R,Bernal A,Szurek B,Lopez C

更新日期：2014-11-01 00:00:00

abstract：:Murashige and Skoog's modified medium in 1% Difco Bacto-agar supplemented with sugar alcohols (sorbsitol, mannitol), growth regulators (1-naphthalenacetic acid, 2,4-dichlorophenoxyacetic acid, benzyladenine, kinetin) and sugars (fructose, glucose, sucrose) induced aposporous gametophytes from pinnae of Pteris vittata ...

journal_title：Plant cell reports

authors： Kwa SH,Wee YC,Loh CS

更新日期：1991-10-01 00:00:00

abstract：:Optimal protoplast yields from cotyledons (2.0×10(6) protoplasts/ 0.5 g tissue) and from true leaves (5.0×10(6) protoplasts/g tissue) of two Cucumis sativus genotypes were obtained following a 16 h digestion with, respectively, 1.25% pectinase+0.5% Cellulysin and 0.5 % pectinase+ 1.0% Cellulysin. Enzyme solutions were...

journal_title：Plant cell reports

authors： Punja ZK,Tang FA,Sarmento GG

更新日期：1990-07-01 00:00:00

abstract：:Brassinosteroids are known to promote cell elongation in a wide range of plant species but their effect on cell division has not been as extensively studied. We examined the effect of brassinolide on the kinetics and final division frequencies of regenerating leaf mesophyll protoplasts of Petunia hybrida Vilm v. Coman...

journal_title：Plant cell reports

authors： Oh MH,Clouse SD

更新日期：1998-09-01 00:00:00

abstract：:The study used Actinidia deliciosa endosperm-derived callus to investigate aspects of the morphology, histology and chemistry of extracellular matrix (ECM) structures in morphogenically stable tissue from long-term culture. SEM showed ECM as a membranous layer or reticulated fibrillar and granular structure linking th...

journal_title：Plant cell reports

authors： Popielarska-Konieczna M,Kozieradzka-Kiszkurno M,Swierczyńska J,Góralski G,Slesak H,Bohdanowicz J

更新日期：2008-07-01 00:00:00

abstract：:Embryogenic cell suspension cultures of garlic (Allium sativum L.) were initiated in liquid medium from friable embryogenic tissue. The optimal parameters for culture maintenance were: (1) an initial cell density of 1-4% (v/v); (2) medium renewal every 14 days and subculturing every 28 days; (3) a low 2,4-dichlorophen...

journal_title：Plant cell reports

authors： Fereol L,Chovelon V,Causse S,Triaire D,Arnault I,Auger J,Kahane R

更新日期：2005-08-01 00:00:00

abstract：:To improve turfgrasses using genetic engineering, we have developed a transformation system in turf-type tall fescue, one of the most important turfgrass species. Embryogenic cell cultures were established after callus induction from embryos of mature seed. The agarose-bead method with nurse cells was used to culture ...

journal_title：Plant cell reports

authors： Ha SB,Wu FS,Thorne TK

更新日期：1992-11-01 00:00:00

abstract：:As part of an ongoing research program dedicated to the understanding of proanthocyanidin (PA) accumulation in Brassica napus seed coat, transgenic rapeseed plants carrying a 2.3-kb fragment of the Arabidopsis thaliana BAN promoter (ProAtBAN) fused to the uidA reporter gene (GUS) were generated. Analysis of these plan...

journal_title：Plant cell reports

authors： Nesi N,Lucas MO,Auger B,Baron C,Lécureuil A,Guerche P,Kronenberger J,Lepiniec L,Debeaujon I,Renard M

更新日期：2009-04-01 00:00:00

abstract：:The expression of three genes that encode proteins involved in peroxisome biogenesis, beta-oxidation and the glyoxylate cycle was studied in Arabidopsis plants by fusing their promoter regions to the reporter gene luciferase. Malate synthase showed an extremely restricted pattern of expression, being detected only in ...

journal_title：Plant cell reports

authors： Charlton WL,Johnson B,Graham IA,Baker A

更新日期：2005-02-01 00:00:00

abstract：KEY MESSAGE:Sustainability and safety aspects of plant cell cultures as food are presented. Applicability of dairy side streams as carbon source and use of natural growth enhancers in cultivation are shown. Biotechnologically produced cellular products are currently emerging to replace and add into the portfolio of agr...

journal_title：Plant cell reports

authors： Häkkinen ST,Nygren H,Nohynek L,Puupponen-Pimiä R,Heiniö RL,Maiorova N,Rischer H,Ritala A

更新日期：2020-12-01 00:00:00