Brassinolide affects the rate of cell division in isolated leaf protoplasts of Petunia hybrida.

Abstract:

:Brassinosteroids are known to promote cell elongation in a wide range of plant species but their effect on cell division has not been as extensively studied. We examined the effect of brassinolide on the kinetics and final division frequencies of regenerating leaf mesophyll protoplasts of Petunia hybrida Vilm v. Comanche. Under optimal auxin and cytokinin conditions, 10-100 nM brassinolide accelerated the time of first cell division by 12 h but had little effect on the final division frequencies after 72-120 h of culture. One micromolar brassinolide showed the same acceleration of first cell division but inhibited the final division frequency by approximately 20%. Under sub-optimal auxin conditions, 10-100 nM brassinolide both accelerated the time of first cell division and dramatically increased the 72- to 120-h final division frequencies. Isolated protoplasts may provide a useful model system to investigate the molecular mechanisms of brassinosteroid action on cell proliferation.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Oh MH,Clouse SD

doi

10.1007/s002990050510

subject

Has Abstract

pub_date

1998-09-01 00:00:00

pages

921-924

issue

12

eissn

0721-7714

issn

1432-203X

pii

10.1007/s002990050510

journal_volume

17

pub_type

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