Use of an ultrasound cell retention system for the size fractionation of somatic embryos of woody species.

abstract：:Separation of stem proteins by sodium dodecyl sulfate polyacrylamide gel electrophoresis permitted classification of 17 celery varieties (Apium graveolens L.) into 7 groups. Several of these groups could be further subdivided into 11 subgroups with gel electrophoresis patterns of 4 isozyme systems. Classification from...

journal_title：Plant cell reports

authors： Quiros CF,McGrath M,Stites JL

更新日期：1987-04-01 00:00:00

abstract：:The first enzyme in the flavonoid pathway, chalcone synthase, is encoded by a gene (CHS) whose expression is normally under developmental control. In our previous studies, an 896-bp promoter region of a flower-specific CHS gene was isolated from Lilium orential 'Sorbonne', and designated as PLoCHS. Here, the PLoCHS pr...

journal_title：Plant cell reports

authors： Liu Y,Lou Q,Xu W,Xin Y,Bassett C,Wang Y

更新日期：2011-12-01 00:00:00

abstract：KEY MESSAGE:Transgenic Arabidopsis and lettuce plants overexpressing AtHSP17.8 showed ABA-hypersensitive but abiotic stress-resistant phenotypes. ABA treatment caused a dramatic induction of early ABA-responsive genes in AtHSP17.8 -overexpressing transgenic lettuce. Plant small heat shock proteins function as chaperone...

journal_title：Plant cell reports

authors： Kim DH,Xu ZY,Hwang I

更新日期：2013-12-01 00:00:00

abstract：:In vitro plant regeneration via embryogenesis was obtained in suspension cultures of Datura innoxia Mill. Embryogenesis was induced in suspension cultures raised from callus of androgenetic origin, using LS liquid medium supplemented with 0.22 mg/l 2,4-D. The total number of embryos formed was variable over time in cu...

journal_title：Plant cell reports

authors： Sharma VK,Jethwani V,Kothari SL

更新日期：1993-08-01 00:00:00

abstract：:A basic developmental framework of the Larix leptolepis Gord male gametophyte is presented in detail by squashing technique. The duration of the meiosis stage was more than 6 months, and included a long diffuse stage during winter. This long duration of the diffuse appearance of the diplotene stage makes L. leptolepis...

journal_title：Plant cell reports

authors： Zhang SG,Yang WH,Qi YC,Li MX,Wang JH,Sun XM,Wang XS,Qi LW

更新日期：2008-11-01 00:00:00

abstract：:Site-specific recombination has been developed into a genetic engineering tool for higher eukaryotes. The manipulation of newly introduced DNA is now possible in the course of genetic transformation procedures, thus making the process more predictable and reliable. Also, a wide variety of chromosomal rearrangements us...

journal_title：Plant cell reports

authors： Lyznik LA,Gordon-Kamm WJ,Tao Y

更新日期：2003-06-01 00:00:00

abstract：:The study used Actinidia deliciosa endosperm-derived callus to investigate aspects of the morphology, histology and chemistry of extracellular matrix (ECM) structures in morphogenically stable tissue from long-term culture. SEM showed ECM as a membranous layer or reticulated fibrillar and granular structure linking th...

journal_title：Plant cell reports

authors： Popielarska-Konieczna M,Kozieradzka-Kiszkurno M,Swierczyńska J,Góralski G,Slesak H,Bohdanowicz J

更新日期：2008-07-01 00:00:00

abstract：:Late Embryogenesis Abundant (LEA) proteins are associated with tolerance to water-related stress. A wheat (Triticum durum) group 2 LEA proteins, known also as dehydrin (DHN-5), has been previously shown to be induced by salt and abscisic acid (ABA). In this report, we analyze the effect of ectopic expression of Dhn-5 ...

journal_title：Plant cell reports

authors： Brini F,Hanin M,Lumbreras V,Amara I,Khoudi H,Hassairi A,Pagès M,Masmoudi K

更新日期：2007-11-01 00:00:00

abstract：:Yucca valida is an important potential source of steroidal saponins closely related to Yucca schidigera, the species that is commercially exploited from the wild as a source of steroidal extracts. Neither of the species has been domesticated mainly because of their slow growth and long life span before harvesting. Her...

journal_title：Plant cell reports

authors： Arce-Montoya M,Rodríguez-Alvarez M,Hernández-González JA,Robert ML

更新日期：2006-08-01 00:00:00

abstract：:The variegated Saintpaulia cultivar Thamires (Saintpaulia sp.), which has pink petals with blue splotches, is generally maintained by leaf cuttings. In contrast, tissue culture-derived progeny of the cultivar showed not only a high percentage of mutants with solid-blue petals but also other solid-color variants, which...

journal_title：Plant cell reports

authors： Sato M,Kawabe T,Hosokawa M,Tatsuzawa F,Doi M

更新日期：2011-05-01 00:00:00

abstract：:A protocol is described for rapid and large-scale propagation of the woody aromatic and medicinal shrub Vitex negundo by in vitro culture of nodal segments from mature plants. Of the three different cytokinins - N6-benzyladenine (BA), kinetin, and thidiazuron - evaluated as supplements to Murashige and Skoog (MS) medi...

journal_title：Plant cell reports

authors： Sahoo Y,Chand PK

更新日期：1998-12-01 00:00:00

abstract：:DNA transfer into zygotic embryos of Eucalyptus globulus by microprojectile bombardment was studied with two devices: a gunpowder apparatus and a compressed-helium system. Using, as a test, the transient expression of a reporter gene, we optimized the physical and biological conditions of bombardment. Six-day-old cult...

journal_title：Plant cell reports

authors： Rochange F,Serrano L,Marque C,Teuliéres C,Boudet AM

更新日期：1995-07-01 00:00:00

abstract：:A simple method using molecular hybridization was devised to quantitatively measure chloroplast DNA synthesis in vivo. Total cellular DNA isolated from Nicotiana tabacum suspension cells, labeled with (3)H-thymidine, was hybridized to nitrocellulose membrane-bound cloned chloroplast DNA (ct DNA) fragments. Colorless, ...

journal_title：Plant cell reports

authors： Cannon G,Heinhorst S,Siedlecki J,Weissbach A

更新日期：1985-04-01 00:00:00

abstract：:Tillering is an important trait of cereal crops that optimizes plant architecture for maximum yield. Teosinte Branched 1 (TB1) is a negative regulator of lateral branching and an inducer of female inflorescence formation in Zea mays (maize). Recent studies indicate that TB1 homologs in Oryza sativa (rice), Sorghum bic...

journal_title：Plant cell reports

authors： Choi MS,Woo MO,Koh EB,Lee J,Ham TH,Seo HS,Koh HJ

更新日期：2012-01-01 00:00:00

abstract：:Most perennial plants must pass through a long juvenile phase of vegetative development before they are capable of flowering. We have developed a method specifying inflorescence proliferation to bypass juvenility and maintain the adult phase. Bamboo ( Bambusa edulis) inflorescences were amplified by incubation in Mura...

journal_title：Plant cell reports

authors： Lin CS,Chen CT,Lin CC,Chang WC

更新日期：2003-06-01 00:00:00

abstract：:Reduced glutathione (GSH) was applied to prevent browning in shoot tip explants of apple (Malus pumila Mill.). Development was compared between shoot tips treated either by (a) dipping into 0.1 mM GSH solution prior to culture (dip treatment), (b) dipping into the GSH solution and transferring to a medium containing 0...

journal_title：Plant cell reports

authors： Nomura K,Matsumoto S,Masuda K,Inoue M

更新日期：1998-05-01 00:00:00

abstract：:Cell suspension cultures of tomato (Lycopersicon esculentum) adapted to growing continuously in the presence of 0.1 mM CdCl2 and accumulated phytochelatins (PCs, poly(γ-Glu-Cys)n-Gly). The highest level of PCs was measured 4 days after inoculation and coincided with the peak of cellular cadmium concentration. At this ...

journal_title：Plant cell reports

authors： Gupta SC,Goldsbrough PB

更新日期：1990-12-01 00:00:00

abstract：:Somatic embroys and subsequent plant regeneration were obtained from isolated leaf epidermis of Gaillardia picta. Abaxial and adaxial epidermal peels (monolayer) from 45 days old aseptic seedlings were isolated and segments measuring 5 mm x 3 mm were cultured on B5 basal medium supplemented with various growth regulat...

journal_title：Plant cell reports

authors： Pillai KG,Rao IU,Rao IV,Ram HY

更新日期：1992-01-01 00:00:00

abstract：:Two modes of endocytosis are known to occur in eucaryotic cells: fluid phase and receptor-mediated endocytosis. Fluid-phase endocytosis in plant cells resembles the retrieval of excess plasma membrane material previously incorporated by exocytosis. Pollen tubes need to carry out strong membrane retrieval due to their ...

journal_title：Plant cell reports

authors： Lisboa S,Scherer GE,Quader H

更新日期：2008-01-01 00:00:00

abstract：KEY MESSAGE:Phosphate starvation increased the production of phenolic acids by inducing the key enzyme genes in a positive feedback pathway in Saliva miltiorrhiza hairy roots. SPX may be involved in this process. Salvia miltiorrhiza is a wildly popular traditional Chinese medicine used for the treatment of coronary hea...

journal_title：Plant cell reports

authors： Liu L,Yang D,Liang T,Zhang H,He Z,Liang Z

更新日期：2016-09-01 00:00:00

abstract：:Scanning electron microscopy has been used to investigate the morphological changes occurring during the development of alfalfa somatic embryos. Embryos were initiated from callus, transferred to suspension culture and matured on solid agar medium. This developmental pattern was compared to that of zygotic embryos dev...

journal_title：Plant cell reports

authors： Xu N,Bewley JD

更新日期：1992-06-01 00:00:00

abstract：:Somatic hybrids were obtained by the symmetric fusion of embryogenic callus cells from tetraploid 'Mame' kumquat [Fortunella hindsii (Champ.) Swing.] and mesophyll cells from diploid trifoliate orange [Poncirus trifoliata (L.) Raf.]. Southern blot analysis of three regenerants revealed that they carried specific rDNA ...

journal_title：Plant cell reports

authors： Miranda M,Motomura T,Ikeda F,Ohgawara T,Saito W,Endo T,Omura M,Moriguchi T

更新日期：1997-03-01 00:00:00

abstract：:Green plants were repeatedly regenerated from suspension-derived protoplasts of Kentucky blue grass (Poa pratensis L.) cv. "Geronimo". One suspension was capable of donating competent protoplasts during long-term culture i. e. 10-16 months after its establishment. The plating efficiency of the protoplasts from three d...

journal_title：Plant cell reports

authors： Nielsen KA,Larsen E,Knudsen E

更新日期：1993-08-01 00:00:00

abstract：:High-frequency bud break and multiple shoots were induced in apical shoot buds and nodal explants ofMorus cathayana, M. lhou andM. serrata on Murashige and Skoog (MS) medium containing 0.5-1.0 mg/l 6-benzylaminopurine (BAP). Addition of gibberellic acid (0.4 mg/l) along with BAP induced faster bud break both in apical...

journal_title：Plant cell reports

authors： Pattnaik SK,Chand PK

更新日期：1997-04-01 00:00:00

abstract：:Globe artichoke represents a natural source of phenolic compounds with dicaffeoylquinic acids along with their biosynthetic precursor chlorogenic acid (5-caffeoylquinic acid) as the predominant molecules. We report the isolation and characterization of a full-length cDNA and promoter of a globe artichoke p-coumaroyl e...

journal_title：Plant cell reports

authors： Moglia A,Comino C,Portis E,Acquadro A,De Vos RC,Beekwilder J,Lanteri S

更新日期：2009-06-01 00:00:00

abstract：:The effect of various inhibitors on differentiation (shoot morphogenesis) in callus cultures of Brassica, Datura and Nicotiana has been investigated. Hormone medium without any inhibitor (control), resulted in 6% shoot formation. Addition of inhibitors such as actinomycin D, cordycepin, abscisic acid, trigonelline and...

journal_title：Plant cell reports

authors： Sethi U,Basu A,Mukherjee SG

更新日期：1990-03-01 00:00:00

abstract：:We report here an efficient Arabidopsis leafdisc transformation protocol yielding an average transformation frequency of 1.6 transgenic shoots per leaf explant 4 weeks after the bacterial infection period. Subsequent cultivation in vitro is such that a high percentage (85-90%) of the primary transformants produces see...

journal_title：Plant cell reports

authors： van der Graaff E,Hooykaas PJ

更新日期：1996-04-01 00:00:00

abstract：:Morphologically normal green plants have reproducibly been regenerated from protoplasts of an Australian wheat (Triticum aestivum cv. Hartog). The protoplasts were isolated from fine embryogenic suspension cultures which were initiated from embryogenic callus. Protoplasts were incubated in a modified liquid MS medium ...

journal_title：Plant cell reports

authors： He DG,Yang YM,Scott KJ

更新日期：1992-02-01 00:00:00

abstract：:A protocol previously developed for B. napus microspore culture was modified to produce embryos from several lines of Brassica campestris. Bud size, genotype, media constituents, and incubation time and temperature were examined. Donor plants were grown in a growth cabinet at a day/night temperature of 10/5°C. Microsp...

journal_title：Plant cell reports

authors： Baillie AM,Epp DJ,Hutcheson D,Keller WA

更新日期：1992-06-01 00:00:00

abstract：KEY MESSAGE:This study established an efficient method of regenerating plants of Ficus lyrata and producing purple-leaved F. lyrata plants through genetic transformation using a VvMybA1 gene of grapevine. ABSTRACT:Ficus lyrata, a species with unique violin- or guitar-shaped leaves, was regenerated from leaf-derived ca...

journal_title：Plant cell reports

authors： Zhao J,Li ZT,Chen J,Henny RJ,Gray DJ,Chen J

更新日期：2013-11-01 00:00:00