Abstract：
:Late Embryogenesis Abundant (LEA) proteins are associated with tolerance to water-related stress. A wheat (Triticum durum) group 2 LEA proteins, known also as dehydrin (DHN-5), has been previously shown to be induced by salt and abscisic acid (ABA). In this report, we analyze the effect of ectopic expression of Dhn-5 cDNA in Arabidopsis thaliana plants and their response to salt and osmotic stress. When compared to wild type plants, the Dhn-5 transgenic plants exhibited stronger growth under high concentrations of NaCl or under water deprivation, and showed a faster recovery from mannitol treatment. Leaf area and seed germination rate decreased much more in wild type than in transgenic plants subjected to salt stress. Moreover, the water potential was more negative in transgenic than in wild type plants. In addition, the transgenic plants have higher proline contents and lower water loss rate under water stress. Also, Na(+) and K(+) accumulate to higher contents in the leaves of the transgenic plants. Our data strongly support the hypothesis that Dhn-5, by its protective role, contributes to an improved tolerance to salt and drought stress through osmotic adjustment.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Brini F,Hanin M,Lumbreras V,Amara I,Khoudi H,Hassairi A,Pagès M,Masmoudi Kdoi
10.1007/s00299-007-0412-xsubject
Has Abstractpub_date
2007-11-01 00:00:00pages
2017-26issue
11eissn
0721-7714issn
1432-203Xjournal_volume
26pub_type
杂志文章abstract：:Cell-wall invertase (CIN) catalyzes the hydrolysis of sucrose into glucose and fructose for the supply of carbohydrates to sink organs via an apoplastic pathway. To study the CIN genes in rice (Oryza sativa L.), we isolated cDNA clones showing amino acid similarity to the plant cell wall invertase proteins from a sear...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-004-0910-z
更新日期：2005-06-01 00:00:00
abstract：:Protoplasts isolated from cell suspensions of albinoMedicago borealis andM. sativa were fused chemically, using two methods, and electrically. Although a small scale method of chemical fusion gave the highest fusion frequency, electrofusion was the superior technique on the basis of throughput of green somatic hybrid ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00735772
更新日期：1989-05-01 00:00:00
abstract：:Leaves excised from shoot cultures of Prunus avium cvs. F12/1 and Charger and genotype 1908, and from five genotypes of P. serotina and two hybrids of P. avium×P. sargentii developed shoots on Woody Plant medium (WPM) supplemented with either benzyladenine (BA) or thidiazuron (TDZ). Regeneration in both P. avium 1908 ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050436
更新日期：1998-04-01 00:00:00
abstract：:Globe artichoke represents a natural source of phenolic compounds with dicaffeoylquinic acids along with their biosynthetic precursor chlorogenic acid (5-caffeoylquinic acid) as the predominant molecules. We report the isolation and characterization of a full-length cDNA and promoter of a globe artichoke p-coumaroyl e...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-009-0695-1
更新日期：2009-06-01 00:00:00
abstract：:Protoplasts derived from oat (Avena sativa L.) suspension culture cells (7 days after subculturing) were electroporated with plasmid DNA containing the Escherichia coli uidA gene encoding the ß-glucuronidase reporter enzyme. Consistently high enzyme activity was observed with electroporation conditions of 500 μF and 1...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00234710
更新日期：1993-05-01 00:00:00
abstract：:This study aimed at establishing a protocol to increase the number of regenerated shoots and to limit the recovery of "escapes" during the regeneration of transgenic flax plants (cv Barbara). Here, we describe how light, adapted media and selection scheme could stimulate the transformation process, the organogenic pot...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-008-0638-2
更新日期：2009-03-01 00:00:00
abstract：:A cDNA clone encoding an ADP-ribosylation factor from potato (Solanum tuberosum L.) was isolated. The nucleotide and deduced amino acid sequences show high homology to known ADP-ribosylation factor sequences from Arabidopsis, yeast, cow and man. In northern blot experiments, all tissues analysed showed expression of t...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00233786
更新日期：1994-12-01 00:00:00
abstract：:An improved protocol forAgrobacterium-mediated transformation of the tomato cultivar Moneymaker was developed by examining the effects of six different factors on the efficiency of transformation. Explant size, explant orientation, gelling agent and plate sealant were found to affect transformation efficiency. Two oth...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF01890875
更新日期：1996-12-01 00:00:00
abstract：:Tissue culture methods for improvement of cotton has lagged seriously compared to other major crops. A method for regeneration of cotton which includes a morphogenetically competent cell suspension was needed to facilitate selection of stress-resistant variants and gene manipulation. Preliminary screening of eight str...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00268487
更新日期：1987-06-01 00:00:00
abstract：:Fully fertile plants with the expected chromosome number 2n=40 were regenerated from excised leaf sections of Glycine clandestina. Shoots formed directly on the explants through organogenesis. Utilizing the same media and procedures fully fertile plants were also regenerated from cotyledon and hypocotyl tissue of the ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269116
更新日期：1986-06-01 00:00:00
abstract：:Arabinogalactan proteins (AGPs) are important proteoglycans regulating somatic embryogenesis in diverse plant species. Embryogenic cells of somatic embryos are covered by special extracellular cell wall layer called extracellular surface matrix network (ECMSN) at their early developmental stages. Here we show that hig...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-007-0429-1
更新日期：2008-02-01 00:00:00
abstract：:Yucca valida is an important potential source of steroidal saponins closely related to Yucca schidigera, the species that is commercially exploited from the wild as a source of steroidal extracts. Neither of the species has been domesticated mainly because of their slow growth and long life span before harvesting. Her...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-006-0144-3
更新日期：2006-08-01 00:00:00
abstract：KEY MESSAGE:Candidate genes were identified and the role of phytohormones such as JA-Me and ABA in the synthesis of S-RNase was emphasized in pear self-incompatibility. Self-incompatibility (SI) occurs widely in flowering plants as an intraspecific reproductive barrier. This phenomenon promotes variation within species...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-017-2194-0
更新日期：2017-11-01 00:00:00
abstract：:Time-course changes in anthocyanin accumulation, phenylalanine ammonia-lyase activity and chalcone synthase activity were examined in roselle callus tissues incubated under different culture conditions. Phenylalanine ammonia-lyase activity was not affected by either the kind of auxin supplemented to the medium or ligh...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269050
更新日期：1989-12-01 00:00:00
abstract：:Cotyledon explants of muskmelon (Cucumis melo L., cv. Amarillo Oro) seedlings were co-cultivated with disarmed Agrobacterium tumefaciens strain LBA4404 that contained the binary vector plasmid pBI121.1. The T-DNA region of this binary vector contains the Nopaline synthase/neomycin phosphotransferase II (NPTII) chimeri...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00239881
更新日期：1994-01-01 00:00:00
abstract：:An efficient plant regeneration system from leaf-derived callus in 6 genotypes of Avena sativa L. has been established. Regenerable callus was induced in the basal 1-2 mm region of 2 to 5 day old seedlings. Plants were regenerated from the regenerable callus and grown to maturity. The frequency of regenerable callus f...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00238604
更新日期：1995-03-01 00:00:00
abstract：:The transit peptide sequence of ibAGP2 (TP2) was found to be capable of targeting protein into the chloroplast in the Arabidopsis protoplasts. TP2 was fused to a beta-glucuronidase (GUS) reporter gene and expressed in Arabidopsis under the control of the ibAGP2 promoter with the aim of dissecting the effect of the tra...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-008-0563-4
更新日期：2008-08-01 00:00:00
abstract：: Mature ovules of Dianthus (Caryophyllaceae) were histologically observed by clearing and serial sectioning to characterize the cells of the embryo sac. The results show that the mature embryo sac was located deep inside the hemitropous ovule due to thick nucellar tissue at the micropylar region. For the isolation of ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050753
更新日期：2000-04-01 00:00:00
abstract：:An efficient micropropagation system for Saussurea involucrata Kar. et Kir., an endangered Chinese medicinal plant, has been developed. Shoot organogenesis occurred from S. involucrata leaf explants inoculated on medium with appropriate supplements of plant growth regulators. 66.0% of shoot regeneration frequency and ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-006-0230-6
更新日期：2007-03-01 00:00:00
abstract：:Tillering is an important trait of cereal crops that optimizes plant architecture for maximum yield. Teosinte Branched 1 (TB1) is a negative regulator of lateral branching and an inducer of female inflorescence formation in Zea mays (maize). Recent studies indicate that TB1 homologs in Oryza sativa (rice), Sorghum bic...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-011-1139-2
更新日期：2012-01-01 00:00:00
abstract：:Previous work has shown that in rice suspension cells, NaCl at 0.4 M can induce Em gene expression and act synergistically with ABA, possibly by potentiating the ABA response pathway through a rate-limiting intermediate (R.M. Bostock and R.S. Quatrano (1992) Plant Physiol., 98, 1356-1363). Since calcium is an intermed...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232374
更新日期：1996-01-01 00:00:00
abstract：:We have established a high-frequency plant regeneration system via somatic embryogenesis from mature seeds of creeping bentgrass (Agrostis palustris Huds). The effects of 2,4-dichlorophenoxyacetic acid (2,4-D), 3.6-dichloroo-anisic acid (dicamba) and 6-benzyladenine (BA) on callus formation and embryogenesis were eval...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00233813
更新日期：1991-11-01 00:00:00
abstract：:LITHOSPERMUM ERYTHRORHIZON: , which are capable of producing red pigments, have been established. The red pigments were formed on the stems of L. erythrorhizon shoots cultured both on solid and in liquid media without phytohormones at 25 °C in the dark. Thin-layer chromatography, high-performance liquid chromatography...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990000237
更新日期：2000-11-01 00:00:00
abstract：:The aim of this study was to optimize the in vitro chromosome-doubling procedure in wheat anther culture. Colchicine, at concentrations of 100-5000 mg/l, was added to the induction medium for 1-5 days. Beneficial effects were obtained with concentrations of 100 and 1000 mg/l colchicine. With time, significant reductio...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050520
更新日期：1998-09-01 00:00:00
abstract：:A novel strategy for the production and maintenance of morphogenic callus for 1 year from mature leaf explants of apple has been developed using micropropagated primary leaves of cv. Golden Delicious. The technique required second generation adventitious buds produced from cultured primary leaves also produced from es...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050446
更新日期：1998-04-01 00:00:00
abstract：:Factors involved in promoting caulogenesis from hypocotyl explants of Phyllanthus fraternus were studied. Hypocotyl explants were cultured on B5 medium supplemented with 2,4-D or NAA in the presence and absence of BAP (at concentrations 0, 10(-7), 10(-6) and 10(-5)M). Adventitious shoots differentiated from callus dev...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232932
更新日期：1994-08-01 00:00:00
abstract：:The present protocol was aimed at establishing a routine transformation procedure via Agrobacterium tumefaciens for an important Oncidium orchid cultivar. An expression vector containing hptII and gusA genes driven by the cauliflower mosaic virus (CaMV) 35S promoter was successfully introduced into the Oncidium orchid...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-003-0614-9
更新日期：2003-06-01 00:00:00
abstract：KEY MESSAGE:Several hypomethylated sites within the Karma region of EgDEF1 and hotspot regions in chromosomes 1, 2, 3, and 5 may be associated with mantling. One of the main challenges faced by the oil palm industry is fruit abnormalities, such as the "mantled" phenotype that can lead to reduced yields. This clonal abn...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-020-02561-9
更新日期：2020-09-01 00:00:00
abstract：:TransgenicRhododendron plants were obtained byAgrobacterium tumefaciens-mediated gene transfer.A. tumefaciens harboring a binary vector that contained the chimeric neomycin phosphotransferase II (NPTII) and (3-glucuronidase (GUS) genes was co-cultivated with stem and leaf segments fromRhododendron tissues culturedin v...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF01275445
更新日期：1996-11-01 00:00:00
abstract：:The growth and sweet diterpene glucosides of Stevia plants propagated by stem-tip cultures were compared with those of the control plants propagated by seeds. There was no significant difference between the two groups both in growth and in chemical composition. As for the contents of sweet diterpene glucosides, howeve...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00270194
更新日期：1984-10-01 00:00:00
