Abstract:
:Scanning electron microscopy has been used to investigate the morphological changes occurring during the development of alfalfa somatic embryos. Embryos were initiated from callus, transferred to suspension culture and matured on solid agar medium. This developmental pattern was compared to that of zygotic embryos developing in ovulo. Somatic embryos begin as distinct pro-embryos within the callus tissue pieces placed in suspension culture. They become globular and heart-shaped while on solid agar medium and then undergo cotyledon elongation and maturation. Somatic embryos develop comparatively slower at early stages of development and faster at the later stages than zygotic embryos. They lack a well-defined suspensor and have a very rough, poorly-differentiated epidermis, the first layer of which is lost after pro-embryo formation. The cotyledons of somatic embryos are multiple and poorlydeveloped; there appears to be a correlation between the amount of surface roughness of the developing embryo and the extent to which polycotyledony occurs.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Xu N,Bewley JDdoi
10.1007/BF00235082subject
Has Abstractpub_date
1992-06-01 00:00:00pages
279-84issue
5-6eissn
0721-7714issn
1432-203Xjournal_volume
11pub_type
杂志文章abstract:: Identifying transformed plant lines carrying the antibiotic resistance marker gene, neomycin phosphotransferase II, requires a more definitive test than the ability of the plant to grow on kanamycin. Although a number of alternative assays have been described, most are cumbersome, time consuming and/or require the us...
journal_title:Plant cell reports
pub_type: 杂志文章
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journal_title:Plant cell reports
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journal_title:Plant cell reports
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Plant cell reports
pub_type: 杂志文章
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更新日期:1986-04-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
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journal_title:Plant cell reports
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pub_type: 杂志文章
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050405
更新日期:1998-03-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
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journal_title:Plant cell reports
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Plant cell reports
pub_type: 杂志文章
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更新日期:1995-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2017-11-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
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更新日期:2006-11-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章,评审
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更新日期:2018-10-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
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更新日期:2021-02-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章,已发布勘误
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更新日期:2019-01-01 00:00:00