Abstract:
:Hairy root cultures of Lithospermum canescens were established using three strains of Agrobacterium rhizogenes: ATCC 15834, LBA 9402 and NCIB 8196. Eight lines resulting from infection with A. rhizogenes ATCC 15834 demonstrated sufficient biomass increase and were submitted to further investigations. The contents of acetylshikonin (ACS) and isobutyrylshikonin (IBS) in transformed hairy roots made up ca. 10% of those observed in natural roots of L. canescens (24.35 and 14.48 mg g(-1) DW, respectively). One line, Lc1-D, produced the largest amounts of ACS (2.72 mg g(-1) DW) and IBS (0.307 mg g(-1) DW). Traces of pyrrolizidine alkaloids (PA), canescine and canescenine, were found in all lines of transformed hairy roots.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Pietrosiuk A,Sykłowska-Baranek K,Wiedenfeld H,Wolinowska R,Furmanowa M,Jaroszyk Edoi
10.1007/s00299-006-0161-2subject
Has Abstractpub_date
2006-10-01 00:00:00pages
1052-8issue
10eissn
0721-7714issn
1432-203Xjournal_volume
25pub_type
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