The shikonin derivatives and pyrrolizidine alkaloids in hairy root cultures of Lithospermum canescens (Michx.) Lehm.

Abstract:

:Hairy root cultures of Lithospermum canescens were established using three strains of Agrobacterium rhizogenes: ATCC 15834, LBA 9402 and NCIB 8196. Eight lines resulting from infection with A. rhizogenes ATCC 15834 demonstrated sufficient biomass increase and were submitted to further investigations. The contents of acetylshikonin (ACS) and isobutyrylshikonin (IBS) in transformed hairy roots made up ca. 10% of those observed in natural roots of L. canescens (24.35 and 14.48 mg g(-1) DW, respectively). One line, Lc1-D, produced the largest amounts of ACS (2.72 mg g(-1) DW) and IBS (0.307 mg g(-1) DW). Traces of pyrrolizidine alkaloids (PA), canescine and canescenine, were found in all lines of transformed hairy roots.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Pietrosiuk A,Sykłowska-Baranek K,Wiedenfeld H,Wolinowska R,Furmanowa M,Jaroszyk E

doi

10.1007/s00299-006-0161-2

subject

Has Abstract

pub_date

2006-10-01 00:00:00

pages

1052-8

issue

10

eissn

0721-7714

issn

1432-203X

journal_volume

25

pub_type

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