In vitro storage of cedar shoot cultures under minimal growth conditions.

Abstract:

:We developed procedures for slow-growth storage of Cedrus atlantica and Cedrus libani microcuttings of juvenile and adult origin, noting factors favouring the extension of subculture intervals. Microcuttings could be stored effectively up to 6 months at 4 degrees C and reduced light intensity, provided that they were grown on a diluted modified MS medium. The addition of 6% mannitol to the storage media affected negatively survival and multiplication capacity of the cultures. The slow-growth storage conditions used in our experiments did not induce remarkable effects on both RAPD variability and average DNA methylation in the species.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Renau-Morata B,Arrillaga I,Segura J

doi

10.1007/s00299-006-0129-2

keywords:

subject

Has Abstract

pub_date

2006-07-01 00:00:00

pages

636-42

issue

7

eissn

0721-7714

issn

1432-203X

journal_volume

25

pub_type

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