Abstract:
:We developed procedures for slow-growth storage of Cedrus atlantica and Cedrus libani microcuttings of juvenile and adult origin, noting factors favouring the extension of subculture intervals. Microcuttings could be stored effectively up to 6 months at 4 degrees C and reduced light intensity, provided that they were grown on a diluted modified MS medium. The addition of 6% mannitol to the storage media affected negatively survival and multiplication capacity of the cultures. The slow-growth storage conditions used in our experiments did not induce remarkable effects on both RAPD variability and average DNA methylation in the species.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Renau-Morata B,Arrillaga I,Segura Jdoi
10.1007/s00299-006-0129-2keywords:
subject
Has Abstractpub_date
2006-07-01 00:00:00pages
636-42issue
7eissn
0721-7714issn
1432-203Xjournal_volume
25pub_type
杂志文章abstract::Cell walls and media were obtained from three kinds of carrot cell culture, namely, embryogenic callus (EC), non-embryogenic callus (NC) and somatic embryos (SE), and analyzed for their sugar content and sugar composition by electrophoresis and gas chromatography. EC formed large cell clusters while NC formed small cl...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232028
更新日期:1995-02-01 00:00:00
abstract::Companion cells are metabolically active and functionally specialized cells that behave as terminals for the transport of materials between phloem and the surrounding tissue. Although previous research has clarified the distinct function of companion cells, it is still largely unknown how plants establish and maintain...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1080-4
更新日期:2011-09-01 00:00:00
abstract::Extracts of cranberry, rich in flavonols and proanthocyanins, inactivated β-glucuronidase (GUS) in assays using either purified bacterial GUS or preparations of transgenic tobacco (Nicotiana tabacum L.) or transgenic cranberry (Vaccinium macrocarpon Ait.) expressing the gusA gene. Histochemical GUS assays produced ran...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050294
更新日期:1997-06-01 00:00:00
abstract:KEY MESSAGE:We describe a steroid-inducible BABY BOOM system that improves plant regeneration in Arabidopsis leaf cultures and yields fertile plants. Regeneration of Arabidopsis thaliana plants for extended periods of time in tissue culture may result in sterile plants. We report here a novel approach for A. thaliana r...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-015-1832-7
更新日期:2015-10-01 00:00:00
abstract::Agrobacterium-mediated transformation frequency is very low with cells from some species such as soybeans. Studies were conducted to investigate the Agrobacterium-mediated transformation frequency in near-isogenic lipoxygenase mutant lines of soybeans, since the nigh level of lipoxygenase activity in soybean embryos m...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00231809
更新日期:1991-03-01 00:00:00
abstract::The expression and functionality of a resveratrol synthase (RS) gene from peanut (Arachis hypogaea) was studied using an Agrobacterium tumefaciens-mediated transient expression system in Nicotiana benthamiana leaves. Functional analysis of RS was demonstrated by tracking its expression during 96 h. To measure the tran...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-008-0664-0
更新日期:2009-04-01 00:00:00
abstract::The introgression of new desirable characters is very necessary in common bean (Phaseolus vulgaris L.)Interspecific hybridization of P. vulgaris L. with P. angustissimus A. Gray, a wild species with narrow leaves, could be successfully achieved for the first time using embryo culture. Sixteen to twenty-three-day old e...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00268593
更新日期:1986-10-01 00:00:00
abstract::Polyclonal antibodies against a part of pea (Pisum sativum L.) LOXG protein have been raised to study the pattern of distribution of related lipoxygenases in pea carpels. The antiserum recognized three lipoxygenase polypeptides in carpels. One of them became undetectable 24 hours after fruit development induction, sug...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232465
更新日期:1996-04-01 00:00:00
abstract::Plant regeneration from immature embryos of peanut (Arachis hypogaea L.) can be accomplished through somatic embryogenesis. The highest frequency of somatic embryo formation occurred on B5 medium plus 0.5-1.0 mg/l picloram. Shoots and plants developed from the somatic embryos only after extended culture on basal mediu...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00778536
更新日期:1989-04-01 00:00:00
abstract::Six sugarbeet (Beta vulgaris L.) lines (GWI-248, SPB-11, MonoHy 55, SMS-1, EL45 and FC607) were tested for regeneration. Shoot cultures were initiated in vitro from naked, sterilized embryos obtained from mature seed. Excised petioles from cultured shoots were plated on Gamborg's B5 medium and four modified Murashige ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00272972
更新日期:1988-01-01 00:00:00
abstract::The gene coding for the green fluorescent protein (GFP) from jellyfish was introduced into conifer tissues by microprojectile bombardment and its transient expression was detected. Two versions of the GFP gene, wild-type GFP and modified GFP with a cryptic intron removed, were directly compared for their expression in...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF01088278
更新日期:1997-02-01 00:00:00
abstract::Mesophyll derived protoplasts of Solanum khasianum Clarke and S. aculeatissimum Jacq. were fused by electrofusion and resulted in interspecific hybrids. In total, 249 calluses were obtained from which more than 65% produced shoots. From the regenerated plantlets 45% could be identified as hybrids by chromosome counts ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00239891
更新日期:1994-01-01 00:00:00
abstract:KEY MESSAGE:An ERF gene, LchERF , was cloned from L. chinense for the first time. Overexpression of LchERF conferred salt stress tolerance in transgenic tobacco lines during seed germination and vegetative growth. Ethylene-responsive transcription factors (ERFs) play important roles in tolerance to biotic and abiotic s...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-014-1678-4
更新日期:2014-12-01 00:00:00
abstract:KEY MESSAGE:We developed a method for detection of mutants in a large number of plants, and found this method to be applicable to detection of a mutant allele at a concentration of 1/1000. Many techniques for SNP analysis have been developed, but most of these techniques are not so sensitive to be used for detection of...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-018-2274-9
更新日期:2018-06-01 00:00:00
abstract::Albinism remains a major problem in cereal improvement programs that rely on doubled haploid (DH) technology, and the factors controlling the phenomenon are not well understood. Here we report on the positive influence of copper on the production of DH plants obtained through microspore embryogenesis (ME) in recalcitr...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-009-0733-z
更新日期:2009-09-01 00:00:00
abstract::Cell suspension cultures of Nicotiana were initiated using conditions designed to selectively favor stable chromosome number. These conditions included use of leaf explants to initiate cultures, growth of cells in culture medium containing 2,4-D, and transfer of cells with short subculture intervals. Four cell lines d...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00272364
更新日期:1982-04-01 00:00:00
abstract::Somatic hybrids were obtained by the symmetric fusion of embryogenic callus cells from tetraploid 'Mame' kumquat [Fortunella hindsii (Champ.) Swing.] and mesophyll cells from diploid trifoliate orange [Poncirus trifoliata (L.) Raf.]. Southern blot analysis of three regenerants revealed that they carried specific rDNA ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF01146782
更新日期:1997-03-01 00:00:00
abstract:KEY MESSAGE:Several hypomethylated sites within the Karma region of EgDEF1 and hotspot regions in chromosomes 1, 2, 3, and 5 may be associated with mantling. One of the main challenges faced by the oil palm industry is fruit abnormalities, such as the "mantled" phenotype that can lead to reduced yields. This clonal abn...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-020-02561-9
更新日期:2020-09-01 00:00:00
abstract::Kidney vetch seedlings were induced to form hairy roots by inoculating their mesocotyls with the wild-type strain 15834 of Agrobacterium rhizogenes or with the A. tumefaciens strain C58C1 containing a binary vector system (the pRiA4b as a helper and the vector pCB1346 bearing a pTiC58-derived isopentenyl transferase g...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233367
更新日期:1992-07-01 00:00:00
abstract:KEY MESSAGE:Phosphate starvation increased the production of phenolic acids by inducing the key enzyme genes in a positive feedback pathway in Saliva miltiorrhiza hairy roots. SPX may be involved in this process. Salvia miltiorrhiza is a wildly popular traditional Chinese medicine used for the treatment of coronary hea...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-016-2007-x
更新日期:2016-09-01 00:00:00
abstract::The variegated Saintpaulia cultivar Thamires (Saintpaulia sp.), which has pink petals with blue splotches, is generally maintained by leaf cuttings. In contrast, tissue culture-derived progeny of the cultivar showed not only a high percentage of mutants with solid-blue petals but also other solid-color variants, which...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1016-z
更新日期:2011-05-01 00:00:00
abstract::The expression of three genes that encode proteins involved in peroxisome biogenesis, beta-oxidation and the glyoxylate cycle was studied in Arabidopsis plants by fusing their promoter regions to the reporter gene luciferase. Malate synthase showed an extremely restricted pattern of expression, being detected only in ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-004-0879-7
更新日期:2005-02-01 00:00:00
abstract:KEY MESSAGE:This study established an efficient method of regenerating plants of Ficus lyrata and producing purple-leaved F. lyrata plants through genetic transformation using a VvMybA1 gene of grapevine. ABSTRACT:Ficus lyrata, a species with unique violin- or guitar-shaped leaves, was regenerated from leaf-derived ca...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-013-1491-5
更新日期:2013-11-01 00:00:00
abstract::The stability of foreign protein production in genetically engineered plant cells was studied. A cultured tobacco cell line was transformed with a chimeric molecule carrying a bacterial gene, ß-glucuronidase (GUS), under plant regulatory sequences. The specific GUS activity was monitored for 294 days with ten independ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00234589
更新日期:1991-12-01 00:00:00
abstract::In an effort to develop a chemically inducible system for insect management, we studied production of Cry1Ab Bacillus thuringiensis (Bt) protein and control of the diamondback moth (DBM), Plutella xylostella L., in inducer-treated and untreated tissues of a broccoli line transformed with a PR-1a/cry1Ab expression cass...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-005-0091-4
更新日期:2006-06-01 00:00:00
abstract::The interior tissues of the alfalfa (Medicago sativa) root nodule differ in form and function from the peripheral layers. The interior tissues are specialized for the fixation of nitrogen in cells infected by rhizobia. In contrast, the peripheral nodule tissues perform roles that assist the interior tissues: they prov...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990000216
更新日期:2000-09-01 00:00:00
abstract::A novel genic male sterile (GMS) line in Brassica napus L., which was identified in 1999, was found to be controlled by a monogenic dominant gene, which we have designated as MDGMS. The microspores of the MDGMS abort before the degradation of the tapetal cell layer. The F1 fertility from any fertile lines crossed with...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-006-0276-5
更新日期:2007-05-01 00:00:00
abstract::DNA fingerprints generated by the Jeffreys' probes, 33.6 and 33.15, indicated the presence of minisatellite-like sequences in the red clover genome. The fingerprints generated by probe 33.6 gave less background and fewer but better defined bands than those obtained with probe 33.15. Assay of a regenerative somaclonal ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00235293
更新日期:1993-12-01 00:00:00
abstract::Pyrolysis mass spectrometry (PyMS) is a rapid, simple, high-resolution analytical method based on thermal degradation of complex material in a vacuum and has been widely applied to the discrimination of closely related microbial strains. Leaf samples of six species and one variety of higher plants (Rosa multiflora, R....
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0714-6
更新日期:2004-02-01 00:00:00
abstract::A protocol for protoplast isolation was developed and tested with five Capsicum genotypes representing two cultivated species, C. annuum and C. chinense. Key variables included growth conditions for source plants and the concentration of mannitol used as osmoticum. Protoplasts isolated from each of the genotypes becam...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00234146
更新日期:1994-04-01 00:00:00