Transient gene expression after electroporation of protoplasts derived from embryogenic maize callus.

abstract：:Transgenic plants offer advantages for biomolecule production because plants can be grown on a large scale and the recombinant macromolecules can be easily harvested and extracted. We introduced an Aspergillus phytase gene into canola (Brassica napus) (line 9412 with low erucic acid and low glucosinolates) by Agrobact...

journal_title：Plant cell reports

authors： Peng RH,Yao QH,Xiong AS,Cheng ZM,Li Y

更新日期：2006-03-01 00:00:00

abstract：:A method to obtain plants from embryogenic callus of Brassica nigra and protoplasts of hypocotyl expiants is described. Callus was initiated on Murashige and Skoog medium containing kinetin (kn) and 2,4-dichlorophenoxy acetic acid (2,4-D). Lowering of auxin induced embryo formation. Supplementation with gibberellic ac...

journal_title：Plant cell reports

authors： Gupta V,Agnihotri A,Jagannathan V

更新日期：1990-12-01 00:00:00

abstract：:A genetic test was performed on seeds from 283 transgenic tobacco plants obtained by T-DNA transformation. Seeds from self-fertilized transgenic plants were germinated on kanamycin-containing medium, and the percentage of seeds which germinated, as well as the ratio of kanamycin-resistant to kanamycin-sensitive seedli...

journal_title：Plant cell reports

authors： Heberle-Bors E,Charvat B,Thompson D,Schernthaner JP,Barta A,Matzke AJ,Matzke MA

更新日期：1988-12-01 00:00:00

abstract：:High-frequency bud break and multiple shoots were induced in apical shoot buds and nodal explants ofMorus cathayana, M. lhou andM. serrata on Murashige and Skoog (MS) medium containing 0.5-1.0 mg/l 6-benzylaminopurine (BAP). Addition of gibberellic acid (0.4 mg/l) along with BAP induced faster bud break both in apical...

journal_title：Plant cell reports

authors： Pattnaik SK,Chand PK

更新日期：1997-04-01 00:00:00

abstract：: Salvia miltiorrhiza contains two groups of biologically active secondary metabolites termed phenolic compounds (e.g. rosmarinic acid) and tanshinones (e.g. cryptotanshinone). Their roles in plant defense responses were examined using a simplified system consisting of a yeast elicitor and a Ti C58 transformed S. milti...

journal_title：Plant cell reports

authors： Chen H,Chen F

更新日期：2000-06-01 00:00:00

abstract：:KEY MESSAGE : Antioxidant activity of seven leafy vegetables and four beans against five reactive oxygen species and reactive nitrogen species was clearly characterized with a protocol using myoglobin as a reporter probe. ...

journal_title：Plant cell reports

authors： Terashima M,Fukukita A,Kodama R,Miki H,Suzuki M,Ikegami M,Tamura N,Yasuda A,Morikawa M,Matsumura S

更新日期：2013-03-01 00:00:00

abstract：:RNA interference (RNAi) is a sequence-specific, posttranscriptional gene silencing (PTGS) process in plants that is mediated by dsRNA homologous to the silenced gene(s). In this study, we report an efficient method to produce dsRNA using a bacterial expression system. Two fragments of the Sugarcane Mosaic Virus (SCMV)...

journal_title：Plant cell reports

authors： Gan D,Zhang J,Jiang H,Jiang T,Zhu S,Cheng B

更新日期：2010-11-01 00:00:00

abstract：:Cultured cells of tobacco (Nicotiana tabacum L. cv Petit Havana) were used to investigate signals regulating the expression of the "model" nuclear gene encoding the alternative oxidase (AOX) (AOX1), the terminal oxidase of the mitochondrial alternative respiratory pathway. Several conditions shown to induce AOX1 mRNA ...

journal_title：Plant cell reports

authors： Gray GR,Maxwell DP,Villarimo AR,McIntosh L

更新日期：2004-12-01 00:00:00

abstract：KEY MESSAGE:Induced mutations in the waxy locus in rice endosperm did not abolish GBSS activity completely. Compensatory mechanisms in endosperm and leaves caused a major reprogramming of the starch biosynthetic machinery. The mutation of genes in the starch biosynthesis pathway has a profound effect on starch quality ...

journal_title：Plant cell reports

authors： Pérez L,Soto E,Farré G,Juanos J,Villorbina G,Bassie L,Medina V,Serrato AJ,Sahrawy M,Rojas JA,Romagosa I,Muñoz P,Zhu C,Christou P

更新日期：2019-03-01 00:00:00

abstract：:The variegated Saintpaulia cultivar Thamires (Saintpaulia sp.), which has pink petals with blue splotches, is generally maintained by leaf cuttings. In contrast, tissue culture-derived progeny of the cultivar showed not only a high percentage of mutants with solid-blue petals but also other solid-color variants, which...

journal_title：Plant cell reports

authors： Sato M,Kawabe T,Hosokawa M,Tatsuzawa F,Doi M

更新日期：2011-05-01 00:00:00

abstract：:Cryopreservation has been shown to improve the frequency of virus elimination - specifically cucumber mosaic virus and banana streak virus - from banana ( Musa spp.) plants. To understand the mode of action of cryopreservation for the eradication of viral particles, we examined the ultrastructure of meristem tips at e...

journal_title：Plant cell reports

authors： Helliot B,Swennen R,Poumay Y,Frison E,Lepoivre P,Panis B

更新日期：2003-03-01 00:00:00

abstract：:Brassinosteroids are known to promote cell elongation in a wide range of plant species but their effect on cell division has not been as extensively studied. We examined the effect of brassinolide on the kinetics and final division frequencies of regenerating leaf mesophyll protoplasts of Petunia hybrida Vilm v. Coman...

journal_title：Plant cell reports

authors： Oh MH,Clouse SD

更新日期：1998-09-01 00:00:00

abstract：:The stability of foreign protein production in genetically engineered plant cells was studied. A cultured tobacco cell line was transformed with a chimeric molecule carrying a bacterial gene, ß-glucuronidase (GUS), under plant regulatory sequences. The specific GUS activity was monitored for 294 days with ten independ...

journal_title：Plant cell reports

authors： Gao J,Lee JM,An G

更新日期：1991-12-01 00:00:00

abstract：:We report the production of taxadiene by transformation of N. benthamiana with a taxadiene synthase gene. The production was significantly increased by an elicitor treatment or metabolic pathway shunting. Paclitaxel (Taxol(®)) was first isolated from the bark of the pacific yew tree as an anticancer agent and has been...

journal_title：Plant cell reports

authors： Hasan MM,Kim HS,Jeon JH,Kim SH,Moon B,Song JY,Shim SH,Baek KH

更新日期：2014-06-01 00:00:00

abstract：KEY MESSAGE:MsCBL4 expression in tobacco enhanced its salt and saline-alkali stress tolerance by regulating calcium accumulation in roots, indicating the important role of calcium metabolism in plant saline-alkali stress tolerance The calcineurin B-like (CBL) family of proteins play important roles in plant abiotic str...

journal_title：Plant cell reports

authors： An Y,Yang XX,Zhang L,Zhang J,Du B,Yao L,Li XT,Guo C

更新日期：2020-08-01 00:00:00

abstract：:The nuclear DNA content of sweet pepper (Capsicum annuum L. var. annuum, 2n = 24) has been measured by flow and image cytometries but the DNA content of each chromosome of this species has not yet been regarded. DNA content of individual chromosomes has been quantified by the flow karyotyping technique, which requires...

journal_title：Plant cell reports

authors： de Abreu IS,Carvalho CR,Clarindo WR

更新日期：2008-07-01 00:00:00

abstract：:Protocols were developed for regeneration and Agrobacterium-mediated transformation of Actinidia eriantha Benth. A. eriantha has a number of features that make it a useful tool for functional genomics in Actinidia: the vines are relatively small and non-vigorous in nature, flowers form all over the vine including on l...

journal_title：Plant cell reports

authors： Wang T,Ran Y,Atkinson RG,Gleave AP,Cohen D

更新日期：2006-05-01 00:00:00

abstract：:In vitro plant regeneration via embryogenesis was obtained in suspension cultures of Datura innoxia Mill. Embryogenesis was induced in suspension cultures raised from callus of androgenetic origin, using LS liquid medium supplemented with 0.22 mg/l 2,4-D. The total number of embryos formed was variable over time in cu...

journal_title：Plant cell reports

authors： Sharma VK,Jethwani V,Kothari SL

更新日期：1993-08-01 00:00:00

abstract：:Echinacea purpurea seedlings were inoculated with several Agrobacterium rhizogenes strains in order to obtain hairy roots. Infection with A. rhizogenes strains LMG63 and LMG150 resulted in callus formation. Upon infection with strains ATCC 15834 and R1601 hairy roots were obtained. Opine detection confirmed transforma...

journal_title：Plant cell reports

authors： Trypsteen M,Van Lijsebettens M,Van Severen R,Van Montagu M

更新日期：1991-06-01 00:00:00

abstract：:Sweet cherry shows S-RNase-based gametophytic self-incompatibility, which prevents self- and cross-fertilization between genetically related individuals. The specificity of the self-incompatible reaction is determined by two genes located in the S-locus. These encode a pistil-expressed ribonuclease (S-RNase) that inhi...

journal_title：Plant cell reports

authors： Wünsch A,Tao R,Hormaza JI

更新日期：2010-07-01 00:00:00

abstract：:Low initiation frequency is one of the main barriers in applying somatic embryogenesis to the clonal production of Pinus species. Factors affecting initiation, including basal medium, plant growth regulators, and Phytagel concentration, have been investigated in loblolly pine (Pinus taeda L.). BM1 basal medium proved ...

journal_title：Plant cell reports

authors： Li XY,Huang FH,Gbur EE Jr

更新日期：1998-02-01 00:00:00

abstract：:Time-course changes in anthocyanin accumulation, phenylalanine ammonia-lyase activity and chalcone synthase activity were examined in roselle callus tissues incubated under different culture conditions. Phenylalanine ammonia-lyase activity was not affected by either the kind of auxin supplemented to the medium or ligh...

journal_title：Plant cell reports

authors： Mizukami H,Tomita K,Ohashi H

更新日期：1989-12-01 00:00:00

abstract：:Anthocyanin is the primary pigment contributing to red, violet, and blue flower color formation. The solubility of anthocyanins is enhanced by UDP glucose: flavonoid 3-O-glucosyltransferase (UFGT) through transfer of the glucosyl moiety from UDP-glucose to 3-hydroxyl group to produce the first stable pigments. To asse...

journal_title：Plant cell reports

authors： Chen WH,Hsu CY,Cheng HY,Chang H,Chen HH,Ger MJ

更新日期：2011-06-01 00:00:00

abstract：:Recent developments in modern biotechnology such as the use of RNA interference (RNAi) have broadened the scope of crop genetic modification. RNAi strategies have led to significant achievements in crop protection against biotic and abiotic stresses, modification of plant traits, and yield improvement. As RNAi-derived...

journal_title：Plant cell reports

authors： Darsan Singh JK,Mat Jalaluddin NS,Sanan-Mishra N,Harikrishna JA

更新日期：2019-12-01 00:00:00

abstract：:To study the developmental transition of chloroplasts from C(3) to C(4) photosynthesis in the terrestrial single-cell C(4) species Bienertia sinuspersici, a regeneration protocol was developed. Stem explant material developed callus either with or without red nodular structures (RNS) when cultured on Murashige-Skoog (...

journal_title：Plant cell reports

authors： Rosnow J,Offermann S,Park J,Okita TW,Tarlyn N,Dhingra A,Edwards GE

更新日期：2011-08-01 00:00:00

abstract：:Rhodamine-6G(R6G), a lipophilic dye which degrades mammalian mitochondria, was shown to arrest the division of Nicotiana protoplasts. When albino recipient-protoplasts were treated with R6G and fused with X-irradiated (green) donor- protoplasts, only green cybrid plants were obtained. The mtDNA of the cybrids was anal...

journal_title：Plant cell reports

authors： Aviv D,Chen R,Galun E

更新日期：1986-06-01 00:00:00

abstract：:Somatic embroys and subsequent plant regeneration were obtained from isolated leaf epidermis of Gaillardia picta. Abaxial and adaxial epidermal peels (monolayer) from 45 days old aseptic seedlings were isolated and segments measuring 5 mm x 3 mm were cultured on B5 basal medium supplemented with various growth regulat...

journal_title：Plant cell reports

authors： Pillai KG,Rao IU,Rao IV,Ram HY

更新日期：1992-01-01 00:00:00

abstract：:The article 'Instability of endosperm development in amphiploids and their parental species in the genus Avena L.', written by Paulina Tomaszewska and Romuald Kosina, was originally published electronically on the publisher's internet portal on 22 May with an incorrect copyright line. As the article was due to be publ...

journal_title：Plant cell reports

authors： Tomaszewska P,Kosina R

更新日期：2019-01-01 00:00:00

abstract：:The growth and sweet diterpene glucosides of Stevia plants propagated by stem-tip cultures were compared with those of the control plants propagated by seeds. There was no significant difference between the two groups both in growth and in chemical composition. As for the contents of sweet diterpene glucosides, howeve...

journal_title：Plant cell reports

authors： Tamura Y,Nakamura S,Fukui H,Tabata M

更新日期：1984-10-01 00:00:00

abstract：:Hypocotyl explants of three leguminous forest tree species, Albizia amara, A. lucida and A. richardiana, have differentiated shoot buds on B5 basal medium. Maximum number of shoots per explant developed on basal medium augmented with 2,4-D (0.1 μM) in A. amara (2) and BA (10 μM) for both A. lucida (2) and A. richardia...

journal_title：Plant cell reports

authors： Tomar UK,Gupta SC

更新日期：1988-10-01 00:00:00