Comparison of Stevia plants grown from seeds, cuttings and stem-tip cultures for growth and sweet diterpene glucosides.

abstract：KEY MESSAGE:High and low resveratrol (Res) contents in two cultivars are correlated with the expression abundance of Myb14 , which could directly activate transcriptional expression of stilbene synthase gene ( STS ). Resveratrol (3,5,4'-trihydroxystilbene) is one of the natural polyphenols produced by secondary metabol...

journal_title：Plant cell reports

authors： Fang L,Hou Y,Wang L,Xin H,Wang N,Li S

更新日期：2014-10-01 00:00:00

abstract：: A system for barley transformation via polyethyleneglycol-mediated DNA uptake into protoplasts isolated directly from scutella and the regeneration of transgenic plants is reported. Scutellum protoplasts (cv. Clipper, an Australian malting cultivar) were co-transformed with plasmids Act 1-DGUS, containing the marker ...

journal_title：Plant cell reports

authors： Nobre J,Davey MR,Lazzeri PA,Cannell ME

更新日期：2000-10-01 00:00:00

abstract：:Chrysosplenium americanum (Saxifragaceae) accumulates a variety of partially O-methylated flavonol glucosides. Because of the semi-aquatic nature of this plant and its extensive contamination with endogenous organisms, the initiation of shoot and callus cultures could only be achieved after (a) using a special surface...

journal_title：Plant cell reports

authors： Brisson L,Ibrahim RK,Rideau M

更新日期：1988-03-01 00:00:00

abstract：:Plum hypocotyl slices were transformed with the coat protein (CP) gene of plum pox virus (PPV-CP) following cocultivation with Agrobacterium tumefaciens containing the plasmid pGA482GG/PPVCP-33. This binary vector carries the PPV-CP gene construct, as well as the chimeric neomycin phosphotransferase and β-glucuronidas...

journal_title：Plant cell reports

authors： Scorza R,Ravelonandro M,Callahan AM,Cordts JM,Fuchs M,Dunez J,Gonsalves D

更新日期：1994-11-01 00:00:00

abstract：:Microspore-derived embryos from Brassica napus L. were dried to less than 15% moisture and stored dry for a minimum of 7 days. Successful plant regeneration was observed when embryos at the cotyledonary stage of development were treated with 50 uM ABA for 7 days prior to desiccation. Solid agar or liquid medium gave s...

journal_title：Plant cell reports

authors： Senaratna T,Kott L,Beversdorf WD,McKersie BD

更新日期：1991-09-01 00:00:00

abstract：:It has been reported that long term cell cultures from maize endosperms are not completely de-differentiated, maintaining some tissue-specific synthesis. We analyzed the expression of zein (the major storage protein of maize seeds) in cultures derived from wildtype and opaque-2 maize endosperms. In wildtype cultures, ...

journal_title：Plant cell reports

authors： Manzocchi LA,Bianchi MW,Viotti A

更新日期：1989-03-01 00:00:00

abstract：:Molecular analysis of gene expression differences between green and red lettuce leaves was performed using the SSH method. BlastX comparisons of subtractive expressed sequence tags (ESTs) indicated that 7.6% of clones encoded enzymes involved in secondary metabolism. Such clones had a particularly high abundance of fl...

journal_title：Plant cell reports

authors： Park JS,Choung MG,Kim JB,Hahn BS,Kim JB,Bae SC,Roh KH,Kim YH,Cheon CI,Sung MK,Cho KJ

更新日期：2007-04-01 00:00:00

abstract：:Approximately 10(6) protoplast-derived cell colonies of sainfoin were stressed with streptomycin and two resistant colonies were recovered. Plants regenerated from these colonies could be recallused on streptomycin-containing medium three years after growth in the absence of the antibiotic.Ultrastructural studies show...

journal_title：Plant cell reports

authors： Hamill JD,Ahuja PS,Davey MR,Cocking EC

更新日期：1986-12-01 00:00:00

abstract：:We have previously developed procedures for the efficient production of sweet orange (Citrus sinensis L. Osbeck) and Carrizo citrange (C. sinensis L. Osbeck×Poncirus trifoliata L. Raf.) transgenic plants using an Agrobacterium tumefaciens-mediated transformation and shoot tip grafting in vitro regeneration system. We ...

journal_title：Plant cell reports

authors： Peña L,Cervera M,Juárez J,Navarro A,Pina JA,Navarro L

更新日期：1997-09-01 00:00:00

abstract：:Various inhibitors of polyamine biosynthesis were used to study the role of polyamines in DNA synthesis and cell division in suspension cultures of Catharanthus roseus (L.) G. Don. Arginine decarboxylase (ADC; EC 4.1.1.19) was the major enzyme responsible for putrescine production. DL α-difluoromethylarginine inhibite...

journal_title：Plant cell reports

authors： Minocha R,Minocha SC,Komamine A,Shortle WC

更新日期：1991-06-01 00:00:00

abstract：:A simple and reliable method was developed for isolating pollen nuclei from Brassica napus and Triticum aestivum for DNA analysis using flow cytometry. The nuclei were released from pollen by ultrasonic treatment. The isolated nuclei following filtration through nylon mesh and a purification procedure were suitable fo...

journal_title：Plant cell reports

authors： Pan G,Zhou Y,Fowke LC,Wang H

更新日期：2004-10-01 00:00:00

abstract：KEY MESSAGE:Prolamin-GFP fusion proteins, expressed under the control of native prolamin promoters, were localized in specific layers of PB-Is. Prolamin-GFP fusion proteins were gradually digested from outside by pepsin digestion. In rice seed endosperm, protein body type I (PB-I) has a layered structure consisting of ...

journal_title：Plant cell reports

authors： Sasou A,Shigemitsu T,Saito Y,Tanaka M,Morita S,Masumura T

更新日期：2016-06-01 00:00:00

abstract：:Isolated ovules from the maize homozygous recessive brown midrib (bm3) were in vitro pollinated by pollen carrying the dominant allele - and the purple embryo marker (PEM). The colour characters of the embryos and kernels corresponded to the results of control pollination and confirmed the process of double fertilizat...

journal_title：Plant cell reports

authors： Havel L,Novák FJ

更新日期：1981-08-01 00:00:00

abstract：:The effect of the oxygen concentration in the aeration gas on regeneration from rice cells in bioreactor cultures was investigated. The efficiency of regeneration in cultures aerated with over 40% oxygen was higher than that in a flask culture. In the case of a culture in which the dissolved oxygen(DO) was saturated b...

journal_title：Plant cell reports

authors： Okamoto A,Kishine S,Hirosawa T,Nakazono A

更新日期：1996-06-01 00:00:00

abstract：:Maize is one of the more important agricultural crops in the world and, under certain conditions, prone to attack from pathogenic fungi. One of these, Aspergillus flavus, produces toxic and carcinogenic metabolites, called aflatoxins, as byproducts of its infection of maize kernels. The alpha-amylase of A. flavus is k...

journal_title：Plant cell reports

authors： Kim YH,Woloshuk CP,Cho EH,Bae JM,Song YS,Huh GH

更新日期：2007-04-01 00:00:00

abstract：:Heat shock transcription factors (Hsfs) are the terminal components of the signal transduction chain mediating the activation of genes responsive to both heat stress and a large number of chemical stressors. This paper aims to clone Hsf from lily and characterize its function by analyses of mRNA expression, transactiv...

journal_title：Plant cell reports

authors： Xin H,Zhang H,Chen L,Li X,Lian Q,Yuan X,Hu X,Cao L,He X,Yi M

更新日期：2010-08-01 00:00:00

abstract：:Site-specific recombination has been developed into a genetic engineering tool for higher eukaryotes. The manipulation of newly introduced DNA is now possible in the course of genetic transformation procedures, thus making the process more predictable and reliable. Also, a wide variety of chromosomal rearrangements us...

journal_title：Plant cell reports

authors： Lyznik LA,Gordon-Kamm WJ,Tao Y

更新日期：2003-06-01 00:00:00

abstract：:The sugarcane (Saccharum officinarum) stress-related MYB transcription factor gene, ScMYBAS1, demonstrated induced response to water deficit and salt stress in our previous study. To elucidate its stress tolerance mechanism at the transcriptional level, we isolated and characterized the promoter (PScMYBAS1, 1,033 bp) ...

journal_title：Plant cell reports

authors： Prabu G,Prasad DT

更新日期：2012-04-01 00:00:00

abstract：:In maize, Mutator transposable elements are either active or silenced within the genome. In response to environmental stress, silenced Mutator elements could be reactivated, leading to changes in genome structure and gene function. However, there is no direct experimental evidence linking environmental stress and Muta...

journal_title：Plant cell reports

authors： Qian Y,Cheng X,Liu Y,Jiang H,Zhu S,Cheng B

更新日期：2010-12-01 00:00:00

abstract：:Cell suspension cultures of cowpea (Vigna sp.) were able to produce extracellular peroxidase. Different growth regulator concentrations induced different peroxidase activity in callus. The crude extracellular medium after four weeks of culture showed higher (6 times) specific peroxidase activity and higher thermo stab...

journal_title：Plant cell reports

authors： Moreno OA,Vazquez-Duhalt R,Nolasco H

更新日期：1990-07-01 00:00:00

abstract：:From the in vitro hairy root cultures of a Datura candida hybrid, 19 tropane alkaloids have been identified using capillary gas-liquid chromatography and mass spectrometry. As in the parent plants, scopolamine is the major alkaloid. Two hitherto undescribed alkaloids have been detected and their structure tentatively ...

journal_title：Plant cell reports

authors： Christen P,Roberts MF,David Phillipson J,Evans WC

更新日期：1990-07-01 00:00:00

abstract：KEY MESSAGE:Phytophthora infestans resistant somatic hybrids of S. × michoacanum (+) S. tuberosum and autofused 4 x S. × michoacanum were obtained. Our material is promising to introgress resistance from S. × michoacanum into cultivated potato background. Solanum × michoacanum (Bitter.) Rydb. (mch) is a wild diploid (2...

journal_title：Plant cell reports

authors： Smyda P,Jakuczun H,Dębski K,Sliwka J,Thieme R,Nachtigall M,Wasilewicz-Flis I,Zimnoch-Guzowska E

更新日期：2013-08-01 00:00:00

abstract：:Seeds are heterogeneous storage reserves with wide array of storage compounds that include various soluble carbohydrates, starch polymer, storage proteins and lipids. These stored reserves comprise 70% of the world's caloric intake in the form of food and animal feed produced through sustainable agriculture, which con...

journal_title：Plant cell reports

authors： Sreenivasulu N

更新日期：2017-05-01 00:00:00

abstract：:Mesophyll derived protoplasts of Solanum khasianum Clarke and S. aculeatissimum Jacq. were fused by electrofusion and resulted in interspecific hybrids. In total, 249 calluses were obtained from which more than 65% produced shoots. From the regenerated plantlets 45% could be identified as hybrids by chromosome counts ...

journal_title：Plant cell reports

authors： Stattmann M,Gerick E,Wenzel G

更新日期：1994-01-01 00:00:00

abstract：KEY MESSAGE:We describe the potato CDPK family and place StCDPK7 as a player in potato response to Phytophthora infestans infection, identifying phenylalanine ammonia lyase as its specific phosphorylation target in vitro. Calcium-dependent protein kinases (CDPKs) decode calcium (Ca2+) signals and activate different sig...

journal_title：Plant cell reports

authors： Fantino E,Segretin ME,Santin F,Mirkin FG,Ulloa RM

更新日期：2017-07-01 00:00:00

abstract：:A new method for obtaining transgenic cowpea was developed using positive selection based on the Escherichia coli 6-phosphomannose isomerase gene as the selectable marker and mannose as the selective agent. Only transformed cells were capable of utilizing mannose as a carbon source. Cotyledonary node explants from 4-d...

journal_title：Plant cell reports

authors： Bakshi S,Saha B,Roy NK,Mishra S,Panda SK,Sahoo L

更新日期：2012-06-01 00:00:00

abstract：:A novel member of the WRKY gene family, designated TcWRKY53, was isolated from a cadmium (Cd)-treated Thlaspi caerulescens cDNA library by differential screening. WRKY proteins specifically bind to W-boxes, which are found in the promoters of many genes involved in defense and response to environmental stress. TcWRKY5...

journal_title：Plant cell reports

authors： Wei W,Zhang Y,Han L,Guan Z,Chai T

更新日期：2008-04-01 00:00:00

abstract：:Plant regeneration in Arachis pintoi was obtained via two developmental pathways: organogenesis and somatic embryogenesis. Organogenic callus cultures were initiated from pieces of leaf on MS medium supplemented with NAA or 2,4-D in combination with BA, KIN or 2iP. The most suitable combination for plant regeneration ...

journal_title：Plant cell reports

authors： Rey HY,Scocchi AM,Gonzalez AM,Mroginski LA

更新日期：2000-09-01 00:00:00

abstract：:The parameters for optimal regeneration of seven commercial strawberry cultivars were tested using a range of explants and culture conditions. Efficient levels of regeneration--those needed to carry out transformation experiments--with the cultivars Calypso, Pegasus, Bolero, Tango and Emily were achieved with leaf dis...

journal_title：Plant cell reports

authors： Passey AJ,Barrett KJ,James DJ

更新日期：2003-01-01 00:00:00

abstract：:The Golgi apparatus in plants is organized as a multitude of individual stacks that are motile in the cytoplasm and in close association with the endoplasmic reticulum (ER) (Boevink et al. in Plant J 15:441-447, 1998). These stacks operate as a sorting centre for cargo molecules, providing modification and redirection...

journal_title：Plant cell reports

authors： Hanton SL,Matheson LA,Chatre L,Rossi M,Brandizzi F

更新日期：2007-09-01 00:00:00