Effect of oxygen-enriched aeration on regeneration of rice (Oryza sativa L.) cell culture.

Abstract:

:The effect of the oxygen concentration in the aeration gas on regeneration from rice cells in bioreactor cultures was investigated. The efficiency of regeneration in cultures aerated with over 40% oxygen was higher than that in a flask culture. In the case of a culture in which the dissolved oxygen(DO) was saturated by aeration with air, the efficiency of regeneration was less than the half that of cultures aerated with 40% oxygen. In cultures with the DO levels controlled at 8,10 and 12 mg/ℓ, the efficiency of regeneration was highest at 12 mg/ℓ. In the oxygenenriched cultures, although cell aggregation was observed and the color of plantlets was relatively pale, more than 90% of them grew into healthy plants.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Okamoto A,Kishine S,Hirosawa T,Nakazono A

doi

10.1007/BF00232217

subject

Has Abstract

pub_date

1996-06-01 00:00:00

pages

731-6

issue

10

eissn

0721-7714

issn

1432-203X

journal_volume

15

pub_type

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