The effects of acetosyringone and pH on Agrobacterium-mediated transformation vary according to plant species.

abstract：:A protocol for protoplast isolation was developed and tested with five Capsicum genotypes representing two cultivated species, C. annuum and C. chinense. Key variables included growth conditions for source plants and the concentration of mannitol used as osmoticum. Protoplasts isolated from each of the genotypes becam...

journal_title：Plant cell reports

authors： Murphy JF,Kyle MM

更新日期：1994-04-01 00:00:00

abstract：:Processes involved in transformation of regenerable soybean (Glycine max (L.) Merrill) immature zygotic cotyledons were studied by assaying the transient expression of the β-glucuronidase gene driven by the 35S promoter and terminated at the 3' end by the soybean 7S storage protein gene. The plasmid containing the chi...

journal_title：Plant cell reports

authors： Moore PJ,Moore AJ,Collins GB

更新日期：1994-07-01 00:00:00

abstract：:Photosynthetically active callus and cell suspension cultures were established from uninfected Lycopersicon peruvianum plants and from uninfected and potato spindle tuber viroid (PSTVd) infected plants of Lycopersicon esculentum cv. Rutgers. Viroid infection was maintained in photoheterotrophic culture on media contai...

journal_title：Plant cell reports

authors： Stöcker S,Guitton MC,Barth A,Mühlbach HP

更新日期：1993-09-01 00:00:00

abstract：:An efficient method was developed using floating membrane rafts (Liferaft(™)) for the micropropagation of Aconitum napellus (Ranunculaceae), a cut flower crop with a low natural propagation rate. This was achieved by introducing shoot tips into culture on Murashige and Skoog's (1962) solid medium, or liquid medium-sup...

journal_title：Plant cell reports

authors： Watad AA,Kochba M,Nissim A,Gaba V

更新日期：1995-03-01 00:00:00

abstract：:MicroRNAs (miRNAs) are small non-coding RNAs that play vital roles in plant abiotic stress responses via cleavage or translational inhibition of their target mRNAs. Populus euphratica is a typical stress-resistant sessile organism that grows in desert areas. Here, we identified sequences of 12 miRNA precursors from 11...

journal_title：Plant cell reports

authors： Qin Y,Duan Z,Xia X,Yin W

更新日期：2011-10-01 00:00:00

abstract：KEY MESSAGE:Our data present H 2 S in a new role, serving as a multi-faceted transducer to different response mechanisms during NO-induced acquisition of tolerance to flooding-induced hypoxia in maize seedling roots. Nitric oxide (NO), serving as a secondary messenger, modulates physiological processes in plants. Recen...

journal_title：Plant cell reports

authors： Peng R,Bian Z,Zhou L,Cheng W,Hai N,Yang C,Yang T,Wang X,Wang C

更新日期：2016-11-01 00:00:00

abstract：:Tree peony (Paeonia suffruticosa Andrews), a woody deciduous shrub, belongs to the section Moutan DC. in the genus of Paeonia of the Paeoniaceae family. To increase the efficiency of breeding, two EST-derived marker systems were developed based on a tree peony expressed sequence tag (EST) database. Using target region...

journal_title：Plant cell reports

authors： Zhang JJ,Shu QY,Liu ZA,Ren HX,Wang LS,De Keyser E

更新日期：2012-02-01 00:00:00

abstract：:The purpose of this study was to develop a new micropropagation system for Tylophora indica, an important medicinal plant in India, using root explants as starting material. Root explants cultured on MS medium supplemented with 6-benzyladenine (BA) or 2-isopentyladenine (2iP) produced organogenic nodular meristemoids ...

journal_title：Plant cell reports

authors： Chaudhuri KN,Ghosh B,Jha S

更新日期：2004-05-01 00:00:00

abstract：:Leaves excised from shoot cultures of Prunus avium cvs. F12/1 and Charger and genotype 1908, and from five genotypes of P. serotina and two hybrids of P. avium×P. sargentii developed shoots on Woody Plant medium (WPM) supplemented with either benzyladenine (BA) or thidiazuron (TDZ). Regeneration in both P. avium 1908 ...

journal_title：Plant cell reports

authors： Hammatt N,Grant NJ

更新日期：1998-04-01 00:00:00

abstract：:The rooting capacities of tips of seedling, juvenile and mature shoots of Sequoiadendron giganteum were compared on different rooting media (inductive and expressive media) after passage on an elongating medium. None of the cuttings rooted when continuously kept on medium containing the auxin NAA and vitamin D2. Perox...

journal_title：Plant cell reports

authors： Berthon JY,Boyer N,Gaspar T

更新日期：1987-10-01 00:00:00

abstract：:Highly viable protoplasts were isolated in large numbers from in vitro-grown leaf and stem tissues of a haploid clone of the apple scion cultivar Golden Delicious (Malus Xdomestica Borkh.). Protoplasts from both sources divided rapidly to give microcallus, when cultured in a modified Kao and Michayluk-based medium. Fo...

journal_title：Plant cell reports

authors： Patat-Ochatt EM,Boccon-Gibod J,Duron M,Ochatt SJ

更新日期：1993-01-01 00:00:00

abstract：:Tillering is an important trait of cereal crops that optimizes plant architecture for maximum yield. Teosinte Branched 1 (TB1) is a negative regulator of lateral branching and an inducer of female inflorescence formation in Zea mays (maize). Recent studies indicate that TB1 homologs in Oryza sativa (rice), Sorghum bic...

journal_title：Plant cell reports

authors： Choi MS,Woo MO,Koh EB,Lee J,Ham TH,Seo HS,Koh HJ

更新日期：2012-01-01 00:00:00

abstract：:An early identification of fusion products was based on the presumed vigorous growth of hybrid calluses after fusion between Solanum brevidens and S. tuberosum leaf protoplasts. The S. brevidens protoplasts were unable to form multicellular colonies under the applied culture conditions. Three size groups of calluses w...

journal_title：Plant cell reports

authors： Polgár Z,Preiszner J,Dudits D,Fehér A

更新日期：1993-05-01 00:00:00

abstract：:Maize is one of the more important agricultural crops in the world and, under certain conditions, prone to attack from pathogenic fungi. One of these, Aspergillus flavus, produces toxic and carcinogenic metabolites, called aflatoxins, as byproducts of its infection of maize kernels. The alpha-amylase of A. flavus is k...

journal_title：Plant cell reports

authors： Kim YH,Woloshuk CP,Cho EH,Bae JM,Song YS,Huh GH

更新日期：2007-04-01 00:00:00

abstract：:The present protocol was aimed at establishing a routine transformation procedure via Agrobacterium tumefaciens for an important Oncidium orchid cultivar. An expression vector containing hptII and gusA genes driven by the cauliflower mosaic virus (CaMV) 35S promoter was successfully introduced into the Oncidium orchid...

journal_title：Plant cell reports

authors： Liau CH,You SJ,Prasad V,Hsiao HH,Lu JC,Yang NS,Chan MT

更新日期：2003-06-01 00:00:00

abstract：:A differential expression study on the Saccharum spp.-Sporisorium scitamineum pathogenic interaction was carried out in the susceptible Ja60-5 and the resistant M31/45 genotypes. Using cDNA-AFLP analysis, a total of 64 transcript-derived fragments (TDFs) was found to be differentially expressed, with the majority (67....

journal_title：Plant cell reports

authors： Lao M,Arencibia AD,Carmona ER,Acevedo R,Rodríguez E,León O,Santana I

更新日期：2008-06-01 00:00:00

abstract：KEY MESSAGE:This study established a rapid method for the gene expression analysis in potato tubers. The use of microtubers would be useful for primary evaluation of tuber-expressed genes. In the development of transgenic potato or of potato with other genome modifications (e.g., genome editing or RNA-directed DNA meth...

journal_title：Plant cell reports

authors： Wakasa Y,Kasai A,Yamazaki M,Tabei Y,Tsuyama M,Igarashi T,Okazaki T,Yamamoto K,Fujihara H,Kanno A,Noro O,Harada T,Akada S

更新日期：2020-11-01 00:00:00

abstract：:Eukaryotes use the ubiquitin-proteasome system to control the abundance of regulatory proteins such as cell-cycle proteins and transcription factors. Over 5% of the Arabidopsis genome encodes for proteins with an apparent functional homology to components of the ubiquitin-proteasome system. This suggests that ubiquiti...

journal_title：Plant cell reports

authors： Schwechheimer C,Schwager K

更新日期：2004-11-01 00:00:00

abstract：:To improve turfgrasses using genetic engineering, we have developed a transformation system in turf-type tall fescue, one of the most important turfgrass species. Embryogenic cell cultures were established after callus induction from embryos of mature seed. The agarose-bead method with nurse cells was used to culture ...

journal_title：Plant cell reports

authors： Ha SB,Wu FS,Thorne TK

更新日期：1992-11-01 00:00:00

abstract：KEY MESSAGE:Overexpression of SoSnRK2.1 improved drought tolerance and growth of tobacco plants. Sucrose non-fermenting1-related protein kinase 2 (SnRK2) is a key enzyme in regulating ABA signal transduction in plants, and it plays a significant role in response to multiple abiotic stresses. In this research, SoSnRK2.1...

journal_title：Plant cell reports

authors： Phan TT,Sun B,Niu JQ,Tan QL,Li J,Yang LT,Li YR

更新日期：2016-09-01 00:00:00

abstract：:The nucleotide sequence of the cucumber (Cucumis sativus L. cv. Baekmibaekdadagi) chloroplast genome was completed. The circular double-stranded DNA, consisting of 155,527 bp, contained a pair of inverted repeat regions (IRa and IRb) of 25,187 bp each, which were separated by small and large single copy regions of 86,...

journal_title：Plant cell reports

authors： Kim JS,Jung JD,Lee JA,Park HW,Oh KH,Jeong WJ,Choi DW,Liu JR,Cho KY

更新日期：2006-04-01 00:00:00

abstract：:The ample supply of O2 proved to be of great importance for berberine production in cell suspension culture ofThalictrum minus, as the specific O2 consumption rate of berberine-producing cells was twice as high as that of non-producing cells. Furthermore, berberine yield increased with increases in the volumetric O2 t...

journal_title：Plant cell reports

authors： Kobayashi Y,Fukui H,Tabata M

更新日期：1989-04-01 00:00:00

abstract：:The sugarcane (Saccharum officinarum) stress-related MYB transcription factor gene, ScMYBAS1, demonstrated induced response to water deficit and salt stress in our previous study. To elucidate its stress tolerance mechanism at the transcriptional level, we isolated and characterized the promoter (PScMYBAS1, 1,033 bp) ...

journal_title：Plant cell reports

authors： Prabu G,Prasad DT

更新日期：2012-04-01 00:00:00

abstract：:Rice plants (Oryza sativa L., Chinsurah Boro II var. Indica) were regenerated from protoplasts isolated from microspore derived cell suspensions. A simple procedure for the establishment of such cell suspension cultures from embryogenic microcallus derived from cultured isolated microspores of Indica-type rice is desc...

journal_title：Plant cell reports

authors： Datta SK,Datta K,Potrykus I

更新日期：1990-09-01 00:00:00

abstract：:KEY MESSAGE : Two soybean cultivars showed markedly different drought tolerance. G6PDH plays a central role in the process of H ( 2 ) O ( 2 ...

journal_title：Plant cell reports

authors： Liu J,Wang X,Hu Y,Hu W,Bi Y

更新日期：2013-03-01 00:00:00

abstract：:Hypocotyl explants of three leguminous forest tree species, Albizia amara, A. lucida and A. richardiana, have differentiated shoot buds on B5 basal medium. Maximum number of shoots per explant developed on basal medium augmented with 2,4-D (0.1 μM) in A. amara (2) and BA (10 μM) for both A. lucida (2) and A. richardia...

journal_title：Plant cell reports

authors： Tomar UK,Gupta SC

更新日期：1988-10-01 00:00:00

abstract：:In the coenobia-forming green alga, Hydrodictyon reticulatum, changes in DNA composition during growth and differentiation of reproductive cells were followed. A "loss" of DNA per nucleus could be attributed to underreplication of an amplified DNA fraction being part of a (GC)-rich satellite band in CsCl gradients of ...

journal_title：Plant cell reports

authors： Ssymank V

更新日期：1983-10-01 00:00:00

abstract：:Gastrodia elata Bl. is an achlorophyllous orchid plant feeding on the fungus Armillaria mellea. The plant lives underground during its life cycle except for florescence. Gastrodianins, members of the superfamily of monocot mannose-binding protein (MBP), have been identified from Gastrodia elata, yet their physiologica...

journal_title：Plant cell reports

authors： Wang HX,Yang T,Zeng Y,Hu Z

更新日期：2007-03-01 00:00:00

abstract：:Donor plants of Hordeum vulgare L. cv. Igri were grown in a conditioned environment to minimise fluctuations in the composition of the microspore population. After isolation different types of microspores were identified within each population, amongst others an embryogenic subpopulation. It was shown that the optimum...

journal_title：Plant cell reports

authors： Hoekstra S,van Zijderveld MH,Heidekamp F,van der Mark F

更新日期：1993-10-01 00:00:00

abstract：:Most infections of plant roots are initiated in the region of elongation; the mechanism for this tissue-specific localization pattern is unknown. In alfalfa expressing PsUGT1 antisense mRNA under the control of the cauliflower mosaic virus (CaMV) 35S promoter, the cell cycle in roots is completed in 48 h instead of 24...

journal_title：Plant cell reports

authors： Woo HH,Hirsch AM,Hawes MC

更新日期：2004-07-01 00:00:00