Microspore culture of Hordeum vulgare L.: the influence of density and osmolality.

abstract：:Rice protoplasts (Oryza sativa L. v Taipei 309) have been transformed to kanamycin resistance following uptake of pCaMVNEO induced by electroporation, PEG and PEG combined with electroporation. Protoplast-derived colonies selected on medium containing 100 μg/ml of kanamycin expressed NPTII activity, and contained DNA ...

journal_title：Plant cell reports

authors： Yang H,Zhang HM,Davey MR,Mulligan BJ,Cocking EC

更新日期：1988-10-01 00:00:00

abstract：:Ethylene emanation rates were assessed from leaf tissues of an embryogenic seed plant (Cycle 0) and regeneration cycle plants selected for enhanced embryogenesis (Cycles I, II and IV). In all experiments, ethylene was assessed from the basal 1 cm portion of the innermost leaf. Ethylene emanation was five-fold higher i...

journal_title：Plant cell reports

authors： Songstad DD,Petracek PD,Sams CE,Conger BV

更新日期：1989-03-01 00:00:00

abstract：:The present protocol was aimed at establishing a routine transformation procedure via Agrobacterium tumefaciens for an important Oncidium orchid cultivar. An expression vector containing hptII and gusA genes driven by the cauliflower mosaic virus (CaMV) 35S promoter was successfully introduced into the Oncidium orchid...

journal_title：Plant cell reports

authors： Liau CH,You SJ,Prasad V,Hsiao HH,Lu JC,Yang NS,Chan MT

更新日期：2003-06-01 00:00:00

abstract：:Mimosa tenuiflora (Willd.) Poiret (Leguminosae) was micropropagated throughin vitro culture of axillary buds on Murashige and Skoog (MS) medium. Shoot formation was achieved when the media were supplemented with 0.1 mg.L(-1) IAA + 3 mg.L(-1) KN.In vitro rooting of regenerated shoots was achieved when 0.1 mg.L(-1) KN w...

journal_title：Plant cell reports

authors： Villarreal ML,Rojas G

更新日期：1996-11-01 00:00:00

abstract：:Plant meiotic prophase I is a complicated process involving the late zygotene and pachytene stages, both crucial for completing synapsis and recombination. Using David Lily (Lilium davidii var. Willmottiae) as our research material, we performed suppression subtractive hybridization to construct EST library of anthers...

journal_title：Plant cell reports

authors： Mu C,Wang S,Zhang S,Pan J,Chen N,Li X,Wang Z,Liu H

更新日期：2011-10-01 00:00:00

abstract：:Through the use of Capsicum chromoplast membranes, we report for the first time the direct methylation of γ-tocopherol into α-tocopherol in the presence of S-adenosylmethionine. Furthermore the S-adenosylmethionine: γ-tocopherol methyltransferase activity has been solubilized. On a protein basis, the activity recovere...

journal_title：Plant cell reports

authors： Camara B,d'Harlingue A

更新日期：1985-02-01 00:00:00

abstract：:The gene coding for the green fluorescent protein (GFP) from jellyfish was introduced into conifer tissues by microprojectile bombardment and its transient expression was detected. Two versions of the GFP gene, wild-type GFP and modified GFP with a cryptic intron removed, were directly compared for their expression in...

journal_title：Plant cell reports

authors： Tian L,Séguin A,Charest PJ

更新日期：1997-02-01 00:00:00

abstract：:The expression of three genes that encode proteins involved in peroxisome biogenesis, beta-oxidation and the glyoxylate cycle was studied in Arabidopsis plants by fusing their promoter regions to the reporter gene luciferase. Malate synthase showed an extremely restricted pattern of expression, being detected only in ...

journal_title：Plant cell reports

authors： Charlton WL,Johnson B,Graham IA,Baker A

更新日期：2005-02-01 00:00:00

abstract：:In this study, we present a method for transient expression of the type III effector AvrGf1 from Xanthomonas citri subsp. citri strain A(w) in grapefruit leaves (Citrus paradisi) via Agrobacterium tumefaciens. The coding sequence of avrGf1 was placed under the control of the constitutive CaMV 35S promoter in the binar...

journal_title：Plant cell reports

authors： Figueiredo JF,Römer P,Lahaye T,Graham JH,White FF,Jones JB

更新日期：2011-07-01 00:00:00

abstract：:Pigeon pea [Cajanus cajan (L.) Millsp.] (Family: Fabaceae) is an important legume crop cultivated across 50 countries in Asia, Africa, and the Americas; and ranks fifth in area among pulses after soybean, common bean, peanut, and chickpea. It is consumed as a major source of protein (21%) to the human population in ma...

journal_title：Plant cell reports

authors： Krishna G,Reddy PS,Ramteke PW,Bhattacharya PS

更新日期：2010-10-01 00:00:00

abstract：:Chloroplast-nucleus communication takes place via processes called anterograde and retrograde signaling pathways. Discovery of the retrograde signaling pathways from the chloroplasts to the nucleus also raised an intriguing proposition that chloroplasts may serve as environmental sensors since multitudes of environmen...

journal_title：Plant cell reports

authors： Pesaresi P,Kim C

更新日期：2019-07-01 00:00:00

abstract：:The use of antibiotic or herbicide resistant genes as selection markers for production of transgenic plants and their continuous presence in the final transgenics has been a serious problem for their public acceptance and commercialization. MAT (multi-auto-transformation) vector system has been one of the different st...

journal_title：Plant cell reports

authors： Khan RS,Ntui VO,Chin DP,Nakamura I,Mii M

更新日期：2011-04-01 00:00:00

abstract：KEY MESSAGE:In GhCFE5 homoeologs, GhCFE5D interacted with more actin homologs and stronger interaction activity than GhCFE5A. GhCFE5D - but not GhCFE5A -overexpression severely disrupted actin cytoskeleton organization and significantly suppressed cell elongation. Homoeologous genes are common in polyploid plants; howe...

journal_title：Plant cell reports

authors： Lv F,Li P,Zhang R,Li N,Guo W

更新日期：2016-04-01 00:00:00

abstract：:Chrysosplenium americanum (Saxifragaceae) accumulates a variety of partially O-methylated flavonol glucosides. Because of the semi-aquatic nature of this plant and its extensive contamination with endogenous organisms, the initiation of shoot and callus cultures could only be achieved after (a) using a special surface...

journal_title：Plant cell reports

authors： Brisson L,Ibrahim RK,Rideau M

更新日期：1988-03-01 00:00:00

abstract：:An improved isolated microspore culture protocol alleviating the recalcitrance typically observed in six-row spring barley was developed by optimizing four key physical factors to increase embryogenesis and reduce albinism. Doubled haploid (DH) plants are completely homozygous individuals that can be generated in just...

journal_title：Plant cell reports

authors： Esteves P,Belzile F

更新日期：2014-06-01 00:00:00

abstract：:A biolistic particle delivery system was used to genetically transform embryogenic tissue of Pinus radiata. The introduced DNA contained a uidA reporter gene under the control of either the tandem CaMV 35S or the artificial Emu promoter, and the npt II selectable marker controlled by the CaMV 35S promoter. The average...

journal_title：Plant cell reports

authors： Walter C,Grace LJ,Wagner A,White DWR,Walden AR,Donaldson SS,Hinton H,Gardner RC,Smith DR

更新日期：1998-04-01 00:00:00

abstract：:This article describes conditions to optimize the yield of viable protoplasts from callus tissue of Asparagus densiflorus cv. Sprengeri and their subsequent regeneration into plantlets. Callus tissue was initiated by culturing spear sections (5-7 mm) on Murashige and Skoog (MS) medium supplemented with 0.8% (wt/vol) B...

journal_title：Plant cell reports

authors： Benmoussa M,Mukhopadhyay S,Desjardins Y

更新日期：1997-12-01 00:00:00

abstract：:TransgenicRhododendron plants were obtained byAgrobacterium tumefaciens-mediated gene transfer.A. tumefaciens harboring a binary vector that contained the chimeric neomycin phosphotransferase II (NPTII) and (3-glucuronidase (GUS) genes was co-cultivated with stem and leaf segments fromRhododendron tissues culturedin v...

journal_title：Plant cell reports

authors： Ueno K,Fukunaga Y,Arisumi K

更新日期：1996-11-01 00:00:00

abstract：:Shoot buds of ginger were successfully encapsulated in 4% sodium alginate gel. Encapsulated buds were germinated in vitro to form roots and shoots. In vitro germination (emergence of sprouts) of encapsulated buds ranged from 16.7% to 81.8% on different media after 5 weeks of incubation. Normal plantlets with an averag...

journal_title：Plant cell reports

authors： Sharma TR,Singh BM,Chauhan RS

更新日期：1994-02-01 00:00:00

abstract：UNLABELLED:The quality and productivity of chrysanthemum are severely compromised by various abiotic stresses. Here, we describe the isolation of CdICE1 from Chrysanthemum dichrum using RACE PCR, which shared identical nucleotide of ICE1 ORF from Chrysanthemum grandiflorum variety 'Jinba'. CdICE1 contains a conserved b...

journal_title：Plant cell reports

authors： Chen L,Chen Y,Jiang J,Chen S,Chen F,Guan Z,Fang W

更新日期：2012-09-01 00:00:00

abstract：:Heading date in rice is an important agronomic trait controlled by several genes. In this study, flowering time of variety Dianjingyou 1 (DJY1) was earlier than a near-isogenic line (named NIL) carried chromosome segment from African rice on chromosome 3S, when grown in both long-day (LD) and short-day (SD) conditions...

journal_title：Plant cell reports

authors： Bian XF,Liu X,Zhao ZG,Jiang L,Gao H,Zhang YH,Zheng M,Chen LM,Liu SJ,Zhai HQ,Wan JM

更新日期：2011-12-01 00:00:00

abstract：:An in vitro propagation technique based on axillary bud proliferation has been developed for matureSapium sebiferum trees. Nodal segments cultured on Murashige and Skoog (MS) medium supplemented with benzyl adenine (1-10 μM and α-naphthaleneacetic acid (0-0.5 μM showed axillary bud proliferation. Shoots proliferated i...

journal_title：Plant cell reports

authors： Siril EA,Dhar U

更新日期：1997-06-01 00:00:00

abstract：:Oil palm is a major economic crop for Malaysia. The major challenges faced by the industry are labor shortage, availability of arable land and unstable commodity price. This has caused the industry to diversify its applications into higher value products besides increasing its yield. While conventional breeding has it...

journal_title：Plant cell reports

authors： Parveez GK,Rasid OA,Masani MY,Sambanthamurthi R

更新日期：2015-04-01 00:00:00

abstract：:Taro bacilliform virus (TaBV) is a pararetrovirus of the genus Badnavirus which infects the monocotyledonous plant, taro ( Colocasia esculenta). A region of the TaBV genome spanning nucleotides 6,281 to 12 (T1200), including the 3' end of open reading frame 3 (ORF 3) and the intergenic region to the end of the tRNA(me...

journal_title：Plant cell reports

authors： Yang IC,Iommarini JP,Becker DK,Hafner GJ,Dale JL,Harding RM

更新日期：2003-08-01 00:00:00

abstract：:Plant regeneration from immature embryos of peanut (Arachis hypogaea L.) can be accomplished through somatic embryogenesis. The highest frequency of somatic embryo formation occurred on B5 medium plus 0.5-1.0 mg/l picloram. Shoots and plants developed from the somatic embryos only after extended culture on basal mediu...

journal_title：Plant cell reports

authors： Ozias-Akins P

更新日期：1989-04-01 00:00:00

abstract：:Leaf, rhizome, and stem explants of Helianthus smithii, a wild relative of the cultivated sunflower, have been tested for their morphogenic potential on media containing a range of hormonal combinations including α-naphthaleneacetic acid and 6-benzylaminopurine (0-2 mg/l). Somatic embryos appeared on the majority of t...

journal_title：Plant cell reports

authors： Laparra H,Stoeva P,Ivanov P,Hahne G

更新日期：1997-07-01 00:00:00

abstract：: Embryogenic nucellar callus cultures of different Citrus species and cultivars growing in hormone-free medium were transferred to medium containing either sucrose or glycerol as the only carbohydrate source. Glycerol has been reported to induce further development of Citrus somatic embryos, while in the presence of s...

journal_title：Plant cell reports

authors： Jiménez VM,Guevara E,Herrera J,Bangerth F

更新日期：2001-01-01 00:00:00

abstract：:Protoplasts derived from oat (Avena sativa L.) suspension culture cells (7 days after subculturing) were electroporated with plasmid DNA containing the Escherichia coli uidA gene encoding the ß-glucuronidase reporter enzyme. Consistently high enzyme activity was observed with electroporation conditions of 500 μF and 1...

journal_title：Plant cell reports

authors： Higgs DC,Colbert JT

更新日期：1993-05-01 00:00:00

abstract：:Euphorbia dulcis endosperm is the site of controlled long lasting endocellular lysis involving segregation and autophagy of portions of the cytoplasm within the endoplasmic reticulum membrane. The lysis products may constitute a food source for the benefit of the early developing embryo. ...

journal_title：Plant cell reports

authors： Muccifora S,Gori P

更新日期：1996-05-01 00:00:00

abstract：:Hairy root culture of Ginseng (Panax ginseng) was established after roots were induced on callus following infection with Agrobacterium rhizogenes. The transformed cultures of ginseng could be subcultured as an axenic root culture in the absence of phytohormones, and grew with extensive lateral branches more rapidly t...

journal_title：Plant cell reports

authors： Yoshikawa T,Furuya T

更新日期：1987-12-01 00:00:00