Production of marker-free disease-resistant potato using isopentenyl transferase gene as a positive selection marker.

abstract：:The production of cybrids, combining nucleus of a species with alien cytoplasmic organelles, is a valuable method used for improvement of various crops. Several citrus cybrids have been created by somatic hybridization. These genotypes are interesting models to analyze the impact of cytoplasmic genome change on nuclea...

journal_title：Plant cell reports

authors： Bassene JB,Froelicher Y,Navarro L,Ollitrault P,Ancillo G

更新日期：2011-06-01 00:00:00

abstract：KEY MESSAGE:A plant-derived 0.3 kb constitutive promoter was obtained from AtTCTP expression analysis, and successfully applied to the expression of a selectable marker gene for production of transgenic creeping bentgrass plants. The isolation and use of an efficient promoter is essential to develop a vector system for...

journal_title：Plant cell reports

authors： Han YJ,Kim YM,Hwang OJ,Kim JI

更新日期：2015-02-01 00:00:00

abstract：:High yields of viable protoplasts were obtained by enzymatic treatment from cotyledonary leaves of various greenhouse grown Phaseolus vulgaris L. cultivars. The protoplasts divided and formed cell clusters in a liquid medium. Early transfer before 10 days in the same medium was necessary for the development of cell co...

journal_title：Plant cell reports

authors： Crepy L,Barros LM,Valente VR

更新日期：1986-04-01 00:00:00

abstract：:Plant regeneration in Arachis pintoi was obtained via two developmental pathways: organogenesis and somatic embryogenesis. Organogenic callus cultures were initiated from pieces of leaf on MS medium supplemented with NAA or 2,4-D in combination with BA, KIN or 2iP. The most suitable combination for plant regeneration ...

journal_title：Plant cell reports

authors： Rey HY,Scocchi AM,Gonzalez AM,Mroginski LA

更新日期：2000-09-01 00:00:00

abstract：:Extracts of cranberry, rich in flavonols and proanthocyanins, inactivated β-glucuronidase (GUS) in assays using either purified bacterial GUS or preparations of transgenic tobacco (Nicotiana tabacum L.) or transgenic cranberry (Vaccinium macrocarpon Ait.) expressing the gusA gene. Histochemical GUS assays produced ran...

journal_title：Plant cell reports

authors： Serres R,McCown B,Zeldin E

更新日期：1997-06-01 00:00:00

abstract：:KEY MESSAGE : Rooting of Artemisia annua increases trichome size on leaves and helps drive the final steps of the biosynthesis of the sesquiterpene antimalarial drug, artemisinin. ...

journal_title：Plant cell reports

authors： Nguyen KT,Towler MJ,Weathers PJ

更新日期：2013-02-01 00:00:00

abstract：:Embryonal-suspensor tissue (EST) of Mediterranean cypress (Cupressus sempervirens L.) was tested for microprojectile-DNA delivery (by the PDS-1000/He device) for different subculture periods (9, 15, and 21 days) using the plasmid vectors pRT99GUS [containing the β-glucuronidase (GUS) and neomycin phosphotransferase (N...

journal_title：Plant cell reports

authors： Lambardi M,Lachance D,Séguin A,Charest PJ

更新日期：1998-12-01 00:00:00

abstract：:The present protocol was aimed at establishing a routine transformation procedure via Agrobacterium tumefaciens for an important Oncidium orchid cultivar. An expression vector containing hptII and gusA genes driven by the cauliflower mosaic virus (CaMV) 35S promoter was successfully introduced into the Oncidium orchid...

journal_title：Plant cell reports

authors： Liau CH,You SJ,Prasad V,Hsiao HH,Lu JC,Yang NS,Chan MT

更新日期：2003-06-01 00:00:00

abstract：:Surface-sterilized leaf disks of horse-radish (Armoracia lapathifolia) were immersed in a suspension of Agrobacterium rhizogenes harboring the root-inducing plasmid (pRi) and cultured on a solid medium. Within about 10 days after inoculation, adventitious roots (hairy roots) emerged from the leaf disks. No roots emerg...

journal_title：Plant cell reports

authors： Noda T,Tanaka N,Mano Y,Nabeshima S,Ohkawa H,Matsui C

更新日期：1987-07-01 00:00:00

abstract：:We describe a simple and efficient protocol for regeneration-transformation of two diploid Medicago lines: the annual M. truncatula R108-1(c3) and the perennial M. sativa ssp. falcata (L.) Arcangeli PI.564263 selected previously as highly embryogenic genotypes. Here, embryo regeneration of R108-1 to complete plants wa...

journal_title：Plant cell reports

authors： Trinh TH,Ratet P,Kondorosi E,Durand P,Kamaté K,Bauer P,Kondorosi A

更新日期：1998-03-01 00:00:00

abstract：KEY MESSAGE:SnYSL3 encodes a plasma-localized transporter delivering various metal-nicotianamine complexes. The expression of SnYSL3 is up-regulated by excess Cd, suggesting an important role for SnYSL3 in response to Cd stress. The Yellow Stripe-Like (YSL) transporters have been proposed to participate in metal uptake...

journal_title：Plant cell reports

authors： Feng S,Tan J,Zhang Y,Liang S,Xiang S,Wang H,Chai T

更新日期：2017-02-01 00:00:00

abstract：:Efficient protoplast culture and plant regeneration of five U.S. rice cultivars (Oryza sativa L.) - Mercury, Lacassine, Maybelle, Cypress, and Lemont - were obtained from suspension cells maintained in modified General Medium. Embryogenic suspension cells were developed from calli grown on the original callus inductio...

journal_title：Plant cell reports

authors： Utomo HS,Croughan SS,Croughan TP

更新日期：1995-01-01 00:00:00

abstract：:Optimum conditions for the isolation and culture of protoplasts from high anthocyanin-producing callus of sweet potato (Ipomoea batatas) were investigated. Growth phase of callus and the ratio of callus-enzyme solution affected the yield of protoplasts. Composition of the medium and protoplast density were examined fo...

journal_title：Plant cell reports

authors： Nishimaki T,Nozue M

更新日期：1985-10-01 00:00:00

abstract：:Cell suspension cultures of Thalictrum minus L. var. hypoleucum Miq. were found to produce a large amount of berberine (400-800 mg/l) when 5-10 μM 6-benzyladenine was added to Linsmaier and Skoog's medium containing 100 μM 1-naphthaleneacetic acid. Most of the berberine produced was released continuously from the cell...

journal_title：Plant cell reports

authors： Nakagawa K,Konagai A,Fukui H,Tabata M

更新日期：1984-12-01 00:00:00

abstract：KEY MESSAGE:Red alga, Gracilariopsis chorda, contains seven carbonic anhydrases that can be grouped into α-, β- and γ-classes. Carbonic anhydrases (CAHs) are metalloenzymes that catalyze the reversible hydration of CO2. These enzymes are present in all living organisms and play roles in various cellular processes, incl...

journal_title：Plant cell reports

authors： Razzak MA,Lee J,Lee DW,Kim JH,Yoon HS,Hwang I

更新日期：2019-02-01 00:00:00

abstract：:The expression and functionality of a resveratrol synthase (RS) gene from peanut (Arachis hypogaea) was studied using an Agrobacterium tumefaciens-mediated transient expression system in Nicotiana benthamiana leaves. Functional analysis of RS was demonstrated by tracking its expression during 96 h. To measure the tran...

journal_title：Plant cell reports

authors： Condori J,Medrano G,Sivakumar G,Nair V,Cramer C,Medina-Bolivar F

更新日期：2009-04-01 00:00:00

abstract：:A system to regenerate fertile rice (Oryza sativa L.) plants (both indica and japonica varieties) from protoplasts isolated from anther-derived embryogenic haploid suspension cultures has been established. Green plants were regenerated from protoplast-derived cell clusters five months after suspension culture initiati...

journal_title：Plant cell reports

authors： Su RC,Rudert ML,Hodges TK

更新日期：1992-12-01 00:00:00

abstract：:Callus cultures and cell suspension cultures derived from Ginkgo biloba L. leaves produced ginkgolidc B. In cell suspension cultures, the production reached a maximum by the 13th day of subculture and followed by a sharp decrease. The medium of Murashige and Skoog induced the highest ginkgolide B content in cultures w...

journal_title：Plant cell reports

authors： Jeon MH,Sung SH,Huh H,Kim YC

更新日期：1995-05-01 00:00:00

abstract：:Somatic embryos and plantlets have been obtained in quite a direct way from mesophyll protoplasts isolated from androgenetic plants of two cultivars ("Loras" and "Tower") of Brassica napus. The procedure consists of four steps. Proembryos were induced in a medium supplemented with a cytokinin and an auxin at comparati...

journal_title：Plant cell reports

authors： Li LC,Kohlenbach HW

更新日期：1982-10-01 00:00:00

abstract：:This article describes conditions to optimize the yield of viable protoplasts from callus tissue of Asparagus densiflorus cv. Sprengeri and their subsequent regeneration into plantlets. Callus tissue was initiated by culturing spear sections (5-7 mm) on Murashige and Skoog (MS) medium supplemented with 0.8% (wt/vol) B...

journal_title：Plant cell reports

authors： Benmoussa M,Mukhopadhyay S,Desjardins Y

更新日期：1997-12-01 00:00:00

abstract：KEY MESSAGE:A novel rice constitutive promoter (P OsCon1 ) was isolated. The molecular mechanism of the promoter activity was investigated. P OsCon1 could be used as an alternative constitutive promoter for crop transgenic engineering. Monocot constitutive promoter is an important resource for crop transgenic engineeri...

journal_title：Plant cell reports

authors： Li J,Xu RF,Qin RY,Ma H,Li H,Zhang YP,Li L,Wei PC,Yang JB

更新日期：2014-10-01 00:00:00

abstract：: A high frequency of embryogenesis and transformation from all parts of flowers of two lines of Medicago truncatula R-108-1 and Jemalong J5 were obtained. Using this flower system, we obtained transgenic plants expressing promoter-uidA gene fusions as well as the gfp living cell color reporter gene. Moreover, this met...

journal_title：Plant cell reports

authors： Kamaté K,Rodriguez-Llorente ID,Scholte M,Durand P,Ratet P,Kondorosi E,Kondorosi A,Trinh TH

更新日期：2000-06-01 00:00:00

abstract：:Three O-methyltransferases which catalyze S-adenosyl-L-methionine (SAM)-dependent O-methylation of licodione (LMT), flavone/flavonol (FMT), and caffeic acid (CMT) were separated from the callus culture of Glycyrrhiza echinata, and characteristic differences between their pH optima and Mg(2+) requirement for activity w...

journal_title：Plant cell reports

authors： Ayabe S,Udagawa A,Iida K,Yoshikawa T,Furuya T

更新日期：1987-02-01 00:00:00

abstract：:Quaternary benzophenanthridine alkaloids (sanguinarine, chelerythrine, chelirubine, chelilutine and macarpine) are specifically induced by cell wall components of Penicillium and Saccharomyces in a colorless strain of Eschscholtzia californica cell suspension cultures. Classical elicitors such as the Phytophthora mega...

journal_title：Plant cell reports

authors： Schumacher HM,Gundlach H,Fiedler F,Zenk MH

更新日期：1987-12-01 00:00:00

abstract：:Embryogenic callus and suspension cultures of carrot (Daucus carota L., cv. Nantaise), growing on/in medium including 1 mg/l 2,4-dichlorophenoxy acetic acid (2,4-D), were transferred to medium with or without this plant growth regulator, to impair or induce, respectively, further development of somatic embryos. The en...

journal_title：Plant cell reports

authors： Jiménez VM,Guevara E,Herrera J,Bangerth F

更新日期：2005-01-01 00:00:00

abstract：:Protoplasts isolated from mesophyll cells of Eruca sativa Lam., cultured on suitable medium, underwent sustained cell divisions to form calli. The plating efficiency was found to be 0.4%. The protoplast-derived calli subsequently produced plantlets through organogenesis (15.71%) and somatic embryogenesis (11.25%). Reg...

journal_title：Plant cell reports

authors： Sikdar SR,Chatterjee G,Das S,Sen SK

更新日期：1987-12-01 00:00:00

abstract：:Head-space volatiles above embryogenicPicea sitchensis (Bong.) Carr. (Sitka spruce) tissues cultured in glass Petri dishes sealed with Parafilm M or cling-film, were captured on Tenax adsorption traps and analysed by gas chromatography / mass spectrometry. Each sealing system released a single major compound into the ...

journal_title：Plant cell reports

authors： Selby C,McRoberts WC,Hamilton JT,Harvey BM

更新日期：1996-12-01 00:00:00

abstract：:Cell suspension cultures of Nicotiana were initiated using conditions designed to selectively favor stable chromosome number. These conditions included use of leaf explants to initiate cultures, growth of cells in culture medium containing 2,4-D, and transfer of cells with short subculture intervals. Four cell lines d...

journal_title：Plant cell reports

authors： Evans DA,Gamborg OL

更新日期：1982-04-01 00:00:00

abstract：:The microprojectile bombardment of immature embryos has proven to be effective in transforming many indica rice varieties. One of the drawbacks of using immature embryos is the requirement of a large number of high quality immature embryos, which itself is a tedious and laborious process. To circumvent these problems,...

journal_title：Plant cell reports

authors： Sivamani E,Shen P,Opalka N,Beachy RN,Fauquet CM

更新日期：1996-01-01 00:00:00

abstract：:Cultured Catharanthus roseus cells exhibit transmembrane ferricyanide reduction through a plasma membrane redox system which may be associated with proton translocation. Evidence shows that endogenous pyridine nucleotides serve as hydrogen donors for the reaction. The proton translocating function of the redox system ...

journal_title：Plant cell reports

authors： Marigo G,Belkoura M

更新日期：1985-12-01 00:00:00