Isolation and culture of protoplasts from high anthocyanin-producing callus of sweet potato.

Abstract:

:Optimum conditions for the isolation and culture of protoplasts from high anthocyanin-producing callus of sweet potato (Ipomoea batatas) were investigated. Growth phase of callus and the ratio of callus-enzyme solution affected the yield of protoplasts. Composition of the medium and protoplast density were examined for protoplast culture.Small colonies were regenerated from the protoplasts. Upon transfer to light a high amount of anthocyanin was accumulated in these colonies.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Nishimaki T,Nozue M

doi

10.1007/BF00269369

subject

Has Abstract

pub_date

1985-10-01 00:00:00

pages

248-51

issue

5

eissn

0721-7714

issn

1432-203X

journal_volume

4

pub_type

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