Somatic embryogenesis and organogenesis in callus cultures of a tree legume - Albizia richardiana King.

Abstract:

:Hypocotyl explants of 1 and 10 mm lengths were excised from 12-day-old in vitro-grown seedlings of Albizia richardiana. The larger pieces, after 40 days of culture, developed shoots along with green calli on B5 + BAP (10(-7)-10(-5)M), while the smaller segments produced only green calli on B5+BAP (10(-7)-10(-4)M) medium. Some of the green calli turned morphogenic and started producing somatic embryos with the 2nd sub-culture and shoots from 7th sub-culture onwards. Calli retained the morphogenic potential even after repeated sub-culturing for over two years. The number of embryos in an embryogenic culture varied from 2 to 20 per callus mass of 5-6.5 cm(3). Sucrose at the 2% level in MS medium was optimal for embryogenesis while 4% was optimal for shoot bud differentiation. Higher levels of sucrose (6-10%) caused browning of green calli and also inhibited differentiation into embryos and shoot buds. By selective sub-culturing of 0.1 cm(3) pieces of embryogenic calli on MS+10(-5)M BAP, 46% of the cultures produced somatic embryos. The latter germinated into plantlets on Knop's medium.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Tomar UK,Gupta SC

doi

10.1007/BF00272982

subject

Has Abstract

pub_date

1988-01-01 00:00:00

pages

70-3

issue

1

eissn

0721-7714

issn

1432-203X

journal_volume

7

pub_type

杂志文章
  • Regulated proteolysis and plant development.

    abstract::Eukaryotes use the ubiquitin-proteasome system to control the abundance of regulatory proteins such as cell-cycle proteins and transcription factors. Over 5% of the Arabidopsis genome encodes for proteins with an apparent functional homology to components of the ubiquitin-proteasome system. This suggests that ubiquiti...

    journal_title:Plant cell reports

    pub_type: 杂志文章,评审

    doi:10.1007/s00299-004-0858-z

    authors: Schwechheimer C,Schwager K

    更新日期:2004-11-01 00:00:00

  • Control of foreign polypeptide localization in specific layers of protein body type I in rice seed.

    abstract:KEY MESSAGE:Prolamin-GFP fusion proteins, expressed under the control of native prolamin promoters, were localized in specific layers of PB-Is. Prolamin-GFP fusion proteins were gradually digested from outside by pepsin digestion. In rice seed endosperm, protein body type I (PB-I) has a layered structure consisting of ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-016-1960-8

    authors: Sasou A,Shigemitsu T,Saito Y,Tanaka M,Morita S,Masumura T

    更新日期:2016-06-01 00:00:00

  • A novel elicitor identified from Magnaporthe oryzae triggers defense responses in tobacco and rice.

    abstract:KEY MESSAGE:Our studies indicate a potential important elicitor candidate which can aid in the fight against a worldwide disease, rice blast. In this study, we report the purification, identification, characterization, and gene cloning of a novel hypersensitive response-inducing protein elicitor (MoHrip2) secreted from...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-014-1663-y

    authors: Chen M,Zhang C,Zi Q,Qiu D,Liu W,Zeng H

    更新日期:2014-11-01 00:00:00

  • Callus formation and induction of a cell suspension culture from Acacia senegal.

    abstract::Cell proliferation has been induced from the cambial zone of a branch of Acacia senegal, kept on the basal Knop and Ball medium in the presence of auxin. Transferred on the more complete nutrient medium of Schenk and Hildebrandt, the colonies gave rise to several cell lines. One of the friable lines, consisting of agg...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00268603

    authors: Hustache G,Barnoud F,Joseleau JP

    更新日期:1986-10-01 00:00:00

  • Physiological and transcriptomic responses of reproductive stage soybean to drought stress.

    abstract:KEY MESSAGE:The dynamic alterations of the physiological and molecular processes in reproductive stage soybean indicated the dramatic impact caused by drought. Drought is a major abiotic stress that limits soybean (Glycine max) production. Most prior studies were focused on either model species or crops that are at the...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-018-2332-3

    authors: Xu C,Xia C,Xia Z,Zhou X,Huang J,Huang Z,Liu Y,Jiang Y,Casteel S,Zhang C

    更新日期:2018-12-01 00:00:00

  • Release and crystallization of berberine in the liquid medium of Thalictrum minus cell suspension cultures.

    abstract::Cell suspension cultures of Thalictrum minus L. var. hypoleucum Miq. were found to produce a large amount of berberine (400-800 mg/l) when 5-10 μM 6-benzyladenine was added to Linsmaier and Skoog's medium containing 100 μM 1-naphthaleneacetic acid. Most of the berberine produced was released continuously from the cell...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00269306

    authors: Nakagawa K,Konagai A,Fukui H,Tabata M

    更新日期:1984-12-01 00:00:00

  • Lanthanide ions are agonists of transient gene expression in rice protoplasts and act in synergy with ABA to increase Em gene expression.

    abstract::Previous work has shown that in rice suspension cells, NaCl at 0.4 M can induce Em gene expression and act synergistically with ABA, possibly by potentiating the ABA response pathway through a rate-limiting intermediate (R.M. Bostock and R.S. Quatrano (1992) Plant Physiol., 98, 1356-1363). Since calcium is an intermed...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00232374

    authors: Rock CD,Quatrano RS

    更新日期:1996-01-01 00:00:00

  • Rapid clonal propagation of three mulberries,Morus cathayana HemsL,M. lhou Koiz. andM. serrata Roxb., through in vitro culture of apical shoot buds and nodal explants from mature trees.

    abstract::High-frequency bud break and multiple shoots were induced in apical shoot buds and nodal explants ofMorus cathayana, M. lhou andM. serrata on Murashige and Skoog (MS) medium containing 0.5-1.0 mg/l 6-benzylaminopurine (BAP). Addition of gibberellic acid (0.4 mg/l) along with BAP induced faster bud break both in apical...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF01092774

    authors: Pattnaik SK,Chand PK

    更新日期:1997-04-01 00:00:00

  • An efficient mannose selection protocol for tomato that has no adverse effect on the ploidy level of transgenic plants.

    abstract::A protocol for Agrobacterium-mediated transformation with mannose selection was developed for cotyledon petiole, hypocotyl and leaf explants of tomato (Lycopersicon esculentum L. Mill). More than 400 transgenic plants from three tomato varieties were selected with 1% mannose in combination with 0.1-0.5% glucose. Avera...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-004-0809-8

    authors: Sigareva M,Spivey R,Willits MG,Kramer CM,Chang YF

    更新日期:2004-10-01 00:00:00

  • Formation of onion bulblets in vitro and viability during medium-term storage.

    abstract:: The influences of light conditions, sucrose and ethylene on in vitro formation and storability of onion (Allium cepa L.) bulblets were studied in various accessions. Light, sucrose and ethylene influenced bulb formation. Storability was primarily enhanced by a high sucrose concentration (100 g/l) in the culture mediu...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s002990000295

    authors: Kästner U,Klahr A,Keller ERJ,Kahane R

    更新日期:2001-02-01 00:00:00

  • Cell differentiation in protoplast cultures from embryogenic callus ofAbies alba L.

    abstract::Protoplasts were isolated from embryogenic callus ofAbies alba L. which originated from immature seeds. The protoplasts were immobilized in alginate beads in order to follow the development of single protoplasts.Surrounding culture medium was modified from Kao and Michayluk (1975). After cell wall regeneration subsequ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00716854

    authors: Lang H,Kohlenbach HW

    更新日期:1989-02-01 00:00:00

  • A moso bamboo transcription factor, Phehdz1, positively regulates the drought stress response of transgenic rice.

    abstract:KEY MESSAGE:78 HD-Zip family genes in Phyllostachys edulis were analyzed. Overexpression of Phehdz1 can improve the drought tolerance of transgenic rice and affect its secondary metabolism. Many studies suggested homeodomain-leucine zipper (HD-Zip) transcription factors are important regulators of plant growth and deve...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-020-02625-w

    authors: Gao Y,Liu H,Zhang K,Li F,Wu M,Xiang Y

    更新日期:2021-01-01 00:00:00

  • Evaluation of microprojectile-mediated DNA delivery and reporter genes for genetic transformation of the Mediterranean cypress (Cupressus sempervirens L.).

    abstract::Embryonal-suspensor tissue (EST) of Mediterranean cypress (Cupressus sempervirens L.) was tested for microprojectile-DNA delivery (by the PDS-1000/He device) for different subculture periods (9, 15, and 21 days) using the plasmid vectors pRT99GUS [containing the β-glucuronidase (GUS) and neomycin phosphotransferase (N...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s002990050556

    authors: Lambardi M,Lachance D,Séguin A,Charest PJ

    更新日期:1998-12-01 00:00:00

  • Improvements in the transformation of Arabidopsis thaliana C24 leaf-discs by Agrobacterium tumefaciens.

    abstract::We report here an efficient Arabidopsis leafdisc transformation protocol yielding an average transformation frequency of 1.6 transgenic shoots per leaf explant 4 weeks after the bacterial infection period. Subsequent cultivation in vitro is such that a high percentage (85-90%) of the primary transformants produces see...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00232455

    authors: van der Graaff E,Hooykaas PJ

    更新日期:1996-04-01 00:00:00

  • Anthocyanin accumulation and changes in activities of phenylalanine ammonia-lyase and chalcone synthase in roselle (Hibiscus sabdariffa L.) callus cultures.

    abstract::Time-course changes in anthocyanin accumulation, phenylalanine ammonia-lyase activity and chalcone synthase activity were examined in roselle callus tissues incubated under different culture conditions. Phenylalanine ammonia-lyase activity was not affected by either the kind of auxin supplemented to the medium or ligh...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00269050

    authors: Mizukami H,Tomita K,Ohashi H

    更新日期:1989-12-01 00:00:00

  • Biotechnology of oil palm: strategies towards manipulation of lipid content and composition.

    abstract::Oil palm is a major economic crop for Malaysia. The major challenges faced by the industry are labor shortage, availability of arable land and unstable commodity price. This has caused the industry to diversify its applications into higher value products besides increasing its yield. While conventional breeding has it...

    journal_title:Plant cell reports

    pub_type: 杂志文章,评审

    doi:10.1007/s00299-014-1722-4

    authors: Parveez GK,Rasid OA,Masani MY,Sambanthamurthi R

    更新日期:2015-04-01 00:00:00

  • Genetic transformation of Cavendish banana (Musa spp. AAA group) cv 'Grand Nain' via microprojectile bombardment.

    abstract:: An effective method has been developed for the stable transformation and regeneration of Cavendish banana (Musa spp. AAA group) cv 'Grand Nain' by microprojectile bombardment. Embryogenic cell suspensions were initiated using immature male flowers as the explant. Cells were co-bombarded with the neomycin phosphotrans...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s002990050004

    authors: Becker DK,Dugdale B,Smith MK,Harding RM,Dale JL

    更新日期:2000-01-01 00:00:00

  • Screening of diploid Medicago sativa germplasm for somatic embryogenesis.

    abstract::Nineteen accessions of diploid Medicago sativa L. belonging to the four subspecies sativa, caerula, falcata and xvaria were screened for their ability to produce somatic embryos on hypocotyl-derived callus. Two medium protocols were used in this study, a three-step sequence with exposure of the callus cultures to a hi...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00269379

    authors: Meijer EG,Brown DC

    更新日期:1985-10-01 00:00:00

  • Isolation, culture and callus regeneration of protoplasts of wild and cultivated Helianthus species.

    abstract::Protoplasts were isolated from seedling roots, hypocotyls, and cotyledons of four cultivars of Helianthus annuus and from leaves of axenic shoot cultures of the wild species H. praecox, H. scaberimus and H. rigidus. Optimal culture conditions were established for the respective protoplast systems, using the agarose be...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00269815

    authors: Bohorova NE,Cocking EC,Power JB

    更新日期:1986-08-01 00:00:00

  • Oxidation-reduction relationship between cycloalkanones and the corresponding cycloalkanols in a cell suspension culture of Nicotiana tabacum.

    abstract::The interconversion between 5 to 8-membered cycloalkanones and the corresponding cycloalkanols by a cell suspension culture of Nicotiana tabacum was studied. It was found that the interconversion is at equilibrium and the balance in the equilibrium depends on the number of carbon atoms in the carbocyclic ring of the s...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00270167

    authors: Suga T,Hamada H,Hirata T

    更新日期:1983-04-01 00:00:00

  • Expression of an immunogenic LTB-based chimeric protein targeting Zaire ebolavirus epitopes from GP1 in plant cells.

    abstract:KEY MESSAGE:An antigenic protein targeting two epitopes from the Zaire ebolavirus GP1 protein was expressed in plant cells rendering an antigen capable of inducing humoral responses in mouse when administered subcutaneously or orally. The 2014 Ebola outbreak made clear that new treatments and prophylactic strategies to...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-016-2088-6

    authors: Ríos-Huerta R,Monreal-Escalante E,Govea-Alonso DO,Angulo C,Rosales-Mendoza S

    更新日期:2017-02-01 00:00:00

  • Somatic embryogenesis and plant regeneration in cotton (Gossypium hirsutum L.).

    abstract::Tissue culture methods for improvement of cotton has lagged seriously compared to other major crops. A method for regeneration of cotton which includes a morphogenetically competent cell suspension was needed to facilitate selection of stress-resistant variants and gene manipulation. Preliminary screening of eight str...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00268487

    authors: Trolinder NL,Goodin JR

    更新日期:1987-06-01 00:00:00

  • Leaf nitrogen dioxide uptake coupling apoplastic chemistry, carbon/sulfur assimilation, and plant nitrogen status.

    abstract::Emission and plant uptake of atmospheric nitrogen oxides (NO + NO(2)) significantly influence regional climate change by regulating the oxidative chemistry of the lower atmosphere, species composition and the recycling of carbon and nutrients, etc. Plant uptake of nitrogen dioxide (NO(2)) is concentration-dependent an...

    journal_title:Plant cell reports

    pub_type: 杂志文章,评审

    doi:10.1007/s00299-010-0898-5

    authors: Hu Y,Sun G

    更新日期:2010-10-01 00:00:00

  • Transient expression and stable transformation of soybean using the jellyfish green fluorescent protein.

    abstract:: Embryogenic soybean [Glycine max (L.) Merrill.] suspension cultures were bombarded with five different gene constructions encoding the jellyfish (Aequorea victoria) green fluorescent protein (GFP). These constructions had altered codon usage compared to the native GFP gene and mutations that increased the solubility ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s002990050702

    authors: Ponappa T,Brzozowski AE,Finer JJ

    更新日期:1999-11-01 00:00:00

  • Introduction of a chimeric gene encoding an oryzacystatin-β-glucuronidase fusion protein into rice protoplasts and regeneration of transformed plants.

    abstract::In order to construct transgenic rice plant with an introduced oryzacystatin (OC)-β-glucuronidase (GUS) fusion gene, we first introduced it into rice protoplasts by electroporation, together with a marker gene conferring hygromycinresistance (pUC-HPH). In a transient assay using the transfected protoplasts, both OC an...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00193714

    authors: Hosoyama H,Irie K,Abe K,Arai S

    更新日期:1995-12-01 00:00:00

  • Expression analysis of defense-related genes in Zingiber (Zingiberaceae) species with different levels of compatibility to the soft rot pathogen Pythium aphanidermatum.

    abstract::Ginger (Zingiber officinale Roscoe) cultivars are susceptible to soft rot disease caused by Pythium aphanidermatum. We analyzed changes in transcript levels of 41 genes in the highly susceptible ginger cultivar varada, a less susceptible wild accession (wild ginger), and a Pythium aphanidermatum-resistant relative, Z....

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-008-0594-x

    authors: Kavitha PG,Thomas G

    更新日期:2008-11-01 00:00:00

  • Defense gene expression in elicitor-treated cell suspension cultures of french bean cv. Imuna.

    abstract::Cell suspension cultures of bean (Phaseolus vulgaris) cv. Imuna accumulated isoflavonoid phytoalexins on exposure to elicitor from the phytopathogenic fungus Colletotrichum lindemuthianum (CL). This was preceeded by rapid increases in the activities of phenylalanine ammonia-lyase (PAL) and chalcone synthase (CHS). How...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00269060

    authors: Ellis JS,Jennings AC,Edwards LA,Mavandad M,Lamb CJ,Dixon RA

    更新日期:1989-12-01 00:00:00

  • In vitro propagation ofAtractylodes lancea.

    abstract::Shoot cultures ofAtractylodes lancea DC. (Compositae) have been established by inoculating the flower bud on Linsmaier-Skoog's medium supplemented with 10(-5) M naphthaleneacetic acid and 10(-5) M 6-benzyl-aminopurine. Shoots were multiplied on a medium containing 10(-6) M 6-benzylaminopurine. Propagated shoots rooted...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF02441005

    authors: Hiraoka N,Yamada N,Kodama T,Tomita Y

    更新日期:1984-06-01 00:00:00

  • Stable transformation of papaya via microprojectile bombardment.

    abstract::Stable transformation of papaya (Carica papaya L.) has been achieved following DNA delivery via high velocity microprojectiles. Three types of embryogenic tissues, including immature zygotic embryos, freshly explanted hypocotyl sections, and somatic embryos derived from both, were bombarded with tungsten particles car...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00232177

    authors: Fitch MM,Manshardt RM,Gonsalves D,Slightom JL,Sanford JC

    更新日期:1990-08-01 00:00:00

  • AtERF15 is a positive regulator of ABA response.

    abstract:KEY MESSAGE:The Arabidopsis AP2/ERF family transcription factor AtERF15 is nuclear-localized and positively regulates ABA and stress responses. Abscisic acid (ABA) is a major plant hormone that controls the expression of hundreds genes involved in various aspects of plant growth and development, such as seed developmen...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-014-1688-2

    authors: Lee SB,Lee SJ,Kim SY

    更新日期:2015-01-01 00:00:00