Callus formation and induction of a cell suspension culture from Acacia senegal.

abstract：:In a study of the 5'-flanking sequence of the Zea mays L. (maize) Glb1 gene in vitro, serial promoter deletions were generated and linked with the β-glucuronidase (GUS) reporter gene. The promoter deletion-GUS fusions were introduced into the maize P3377 cell line by particle bombardment. GUS assays indicated that tre...

journal_title：Plant cell reports

authors： Liu S,Kriz A,Duncan D,Widholm J

更新日期：1998-05-01 00:00:00

abstract：KEY MESSAGE:Autotetraploid Arabidopsis line esd and 4COL exhibit enhanced tolerance to Cu stress by enhancing activation of antioxidative defenses, altering expression of genes related to Cu transport, chelation, and ABA-responsive. Autopolyploidy is ubiquitous among angiosperms and often results in better adaptation t...

journal_title：Plant cell reports

authors： Li M,Xu G,Xia X,Wang M,Yin X,Zhang B,Zhang X,Cui Y

更新日期：2017-10-01 00:00:00

abstract：:The expression of three genes that encode proteins involved in peroxisome biogenesis, beta-oxidation and the glyoxylate cycle was studied in Arabidopsis plants by fusing their promoter regions to the reporter gene luciferase. Malate synthase showed an extremely restricted pattern of expression, being detected only in ...

journal_title：Plant cell reports

authors： Charlton WL,Johnson B,Graham IA,Baker A

更新日期：2005-02-01 00:00:00

abstract：:Salt tolerant cell lines have been selected from Medicago sativa, by a single step selection process on tissue culture medium containing 1% NaCl. Plants regenerated from these lines show improved salt tolerance compared to parent plants. The regenerated plants are vigorous, have flowered and are self fertile. The cell...

journal_title：Plant cell reports

authors： Winicov I

更新日期：1991-12-01 00:00:00

abstract：:Chrysosplenium americanum (Saxifragaceae) accumulates a variety of partially O-methylated flavonol glucosides. Because of the semi-aquatic nature of this plant and its extensive contamination with endogenous organisms, the initiation of shoot and callus cultures could only be achieved after (a) using a special surface...

journal_title：Plant cell reports

authors： Brisson L,Ibrahim RK,Rideau M

更新日期：1988-03-01 00:00:00

abstract：:A restricted part of the undeveloped stem of the garlic clove, called the "stem disc", which is just under the basement of the immature foliage leaves, proved to be a very potent explant for the micropropagation of garlic. Twenty to thirty tissue-cultured shoots consistently were differentiated from a single clove dur...

journal_title：Plant cell reports

authors： Ayabe M,Sumi S

更新日期：1998-07-01 00:00:00

abstract：:The use of antibiotic or herbicide resistant genes as selection markers for production of transgenic plants and their continuous presence in the final transgenics has been a serious problem for their public acceptance and commercialization. MAT (multi-auto-transformation) vector system has been one of the different st...

journal_title：Plant cell reports

authors： Khan RS,Ntui VO,Chin DP,Nakamura I,Mii M

更新日期：2011-04-01 00:00:00

abstract：:Autoclaving is a standard procedure for sterilizing nutrient media for plant tissue cultures. Most tissue cultures are grown at pH 5.2 to 5.8 with pH adjustments being made prior to autoclaving. This paper reports that there are significant differences between initial pH levels and pH levels following autoclaving, par...

journal_title：Plant cell reports

authors： Skirvin RM,Chu MC,Mann ML,Young H,Sullivan J,Fermanian T

更新日期：1986-08-01 00:00:00

abstract：:Morphologically normal and fertile transgenic plants of mungbean with two transgenes, bar and alpha-amylase inhibitor, have been developed for the first time. Cotyledonary node explants were transformed by cocultivation with Agrobacterium tumefaciens strain EHA105 harboring a binary vector pKSB that carried bialaphos ...

journal_title：Plant cell reports

authors： Sonia,Saini R,Singh RP,Jaiwal PK

更新日期：2007-02-01 00:00:00

abstract：:Protoplasts isolated from mesophyll cells of Eruca sativa Lam., cultured on suitable medium, underwent sustained cell divisions to form calli. The plating efficiency was found to be 0.4%. The protoplast-derived calli subsequently produced plantlets through organogenesis (15.71%) and somatic embryogenesis (11.25%). Reg...

journal_title：Plant cell reports

authors： Sikdar SR,Chatterjee G,Das S,Sen SK

更新日期：1987-12-01 00:00:00

abstract：:Pyrolysis mass spectrometry (PyMS) is a rapid, simple, high-resolution analytical method based on thermal degradation of complex material in a vacuum and has been widely applied to the discrimination of closely related microbial strains. Leaf samples of six species and one variety of higher plants (Rosa multiflora, R....

journal_title：Plant cell reports

authors： Kim SW,Ban SH,Chung HJ,Choi DW,Choi PS,Yoo OJ,Liu JR

更新日期：2004-02-01 00:00:00

abstract：KEY MESSAGE:Overexpressing TaUb2 promoted stem growth and resulted in early flowering in transgenic tobacco plants. Ubiquitin are involved in the production, metabolism and proper function of gibberellin. The ubiquitin-26S proteasome system (UPS), in which ubiquitin (Ub) functions as a marker, is a post-translational r...

journal_title：Plant cell reports

authors： Wang GK,Zhang M,Gong JF,Guo QF,Feng YN,Wang W

更新日期：2012-12-01 00:00:00

abstract：: Rooted regenerated shoots obtained from leaf and nodal segments of Rosmarinus officinalis were grown on a basal nutrient medium for 9 weeks. The regenerants were shown by means of HPLC and mass spectrometry to contain carnosic acid, a diterpenoid with antioxidant and medicinal properties. Five-week-old nodular green ...

journal_title：Plant cell reports

authors： Caruso JL,Callahan J,DeChant C,Jayasimhulu K,Winget GD

更新日期：2000-04-01 00:00:00

abstract：: Cells of a suspension culture of Doritaenopsis cv. New Toyohashi were placed in a mixture of 2 M glycerol and 0.4 M sucrose for 15 min at room temperature and then dehydrated with a vitrification solution (PVS2) for 1-3 h on ice and plunged into liquid nitrogen. The highest viability (64% by 2,3,5-triphenyltetrazoliu...

journal_title：Plant cell reports

authors： Tsukazaki H,Mii M,Tokuhara K,Ishikawa K

更新日期：2000-12-01 00:00:00

abstract：:Highly viable protoplasts were isolated in large numbers from in vitro-grown leaf and stem tissues of a haploid clone of the apple scion cultivar Golden Delicious (Malus Xdomestica Borkh.). Protoplasts from both sources divided rapidly to give microcallus, when cultured in a modified Kao and Michayluk-based medium. Fo...

journal_title：Plant cell reports

authors： Patat-Ochatt EM,Boccon-Gibod J,Duron M,Ochatt SJ

更新日期：1993-01-01 00:00:00

abstract：:The full-length cDNAs of eight S ribonucleases (S-RNases) were cloned from stylar RNA of European pear cultivars that could not be characterized by the cleaved amplified polymorphic sequences (CAPS) marker system for genotyping European pear cultivars harboring nine S alleles Sa, Sb, Sd, Se, Sh, Sk, Sl, Sq, and Sr. Co...

journal_title：Plant cell reports

authors： Moriya Y,Yamamoto K,Okada K,Iwanami H,Bessho H,Nakanishi T,Takasaki T

更新日期：2007-03-01 00:00:00

abstract：KEY MESSAGE:We describe the potato CDPK family and place StCDPK7 as a player in potato response to Phytophthora infestans infection, identifying phenylalanine ammonia lyase as its specific phosphorylation target in vitro. Calcium-dependent protein kinases (CDPKs) decode calcium (Ca2+) signals and activate different sig...

journal_title：Plant cell reports

authors： Fantino E,Segretin ME,Santin F,Mirkin FG,Ulloa RM

更新日期：2017-07-01 00:00:00

abstract：:Shoot cultures of Allium sativum L. were established from stem-tip and scape-tip. Various combinations of media, growth regulators were compared. The best establishment and proliferation of stem-tip and scape-tip explants were obtained on MS medium containing 0.5 mg l(-1) NAA and 0.5 mg l(-1) KT. The roots were produc...

journal_title：Plant cell reports

authors： Ma Y,Wang HL,Zhang CJ,Kang YQ

更新日期：1994-11-01 00:00:00

abstract：:Protoplasts of sugarbeet (Beta vulgaris L.) were isolated from cell suspension cultures and cultured in modified PGo medium. Conditions required for the efficient division of the protoplasts were investigated.The optimal combination of phytohormones was found to be 1 mg/l NAA, 0.2 mg/l 2,4-D, 0.5 mg/l zeatin. Protopla...

journal_title：Plant cell reports

authors： Szabados L,Gaggero C

更新日期：1985-08-01 00:00:00

abstract：KEY MESSAGE:A chalcone synthase gene ( PaCHS ) was isolated and functionally characterized from liverwort. The ectopic expression of PaCHS in Marchantia paleacea callus raised the flavonoids content. Chalcone synthase (CHS; EC 2.3.1.74) is pivotal for the biosynthesis of flavonoid and anthocyanin pigments in plants. It...

journal_title：Plant cell reports

authors： Yu HN,Wang L,Sun B,Gao S,Cheng AX,Lou HX

更新日期：2015-02-01 00:00:00

abstract：:The sequences of the 3' untranslated region (UTR) of the manganese superoxide dismutase (MnSOD) genes in wheat (Triticum aestivum) were found to be quite variable with different predicted thermostabilities. The degradation rates of the 3' UTR variants and the coding region were measured following exposure to endogenou...

journal_title：Plant cell reports

authors： Baek KH,Skinner DZ

更新日期：2006-03-01 00:00:00

abstract：:Time-course changes in anthocyanin accumulation, phenylalanine ammonia-lyase activity and chalcone synthase activity were examined in roselle callus tissues incubated under different culture conditions. Phenylalanine ammonia-lyase activity was not affected by either the kind of auxin supplemented to the medium or ligh...

journal_title：Plant cell reports

authors： Mizukami H,Tomita K,Ohashi H

更新日期：1989-12-01 00:00:00

abstract：:Fusarium head blight (FHB), caused primarily by Fusarium graminearum, is a major disease problem in wheat (Triticum aestivum). Genetic engineering holds significant potential to enhance FHB resistance in wheat. Due to the requirement of screening for FHB resistance on flowers at anthesis, the number of screens carried...

journal_title：Plant cell reports

authors： Mackintosh CA,Garvin DF,Radmer LE,Heinen SJ,Muehlbauer GJ

更新日期：2006-04-01 00:00:00

abstract：:Optimal protoplast yields from cotyledons (2.0×10(6) protoplasts/ 0.5 g tissue) and from true leaves (5.0×10(6) protoplasts/g tissue) of two Cucumis sativus genotypes were obtained following a 16 h digestion with, respectively, 1.25% pectinase+0.5% Cellulysin and 0.5 % pectinase+ 1.0% Cellulysin. Enzyme solutions were...

journal_title：Plant cell reports

authors： Punja ZK,Tang FA,Sarmento GG

更新日期：1990-07-01 00:00:00

abstract：KEY MESSAGE:Cadmium sensitivity in sultr1;1 - sultr1;2 double mutant with limiting sulfate supply is attributed to the decreased glutathione content that affected oxidative defense but not phytochelatins' synthesis. In plants, glutathione (GSH) homeostasis plays pivotal role in cadmium (Cd) detoxification. GSH is synth...

journal_title：Plant cell reports

authors： Liu X,Wu FH,Li JX,Chen J,Wang GH,Wang WH,Hu WJ,Gao LJ,Wang ZL,Chen JH,Simon M,Zheng HL

更新日期：2016-02-01 00:00:00

abstract：KEY MESSAGE:High and low resveratrol (Res) contents in two cultivars are correlated with the expression abundance of Myb14 , which could directly activate transcriptional expression of stilbene synthase gene ( STS ). Resveratrol (3,5,4'-trihydroxystilbene) is one of the natural polyphenols produced by secondary metabol...

journal_title：Plant cell reports

authors： Fang L,Hou Y,Wang L,Xin H,Wang N,Li S

更新日期：2014-10-01 00:00:00

abstract：:The nucleotide sequence of the cucumber (Cucumis sativus L. cv. Baekmibaekdadagi) chloroplast genome was completed. The circular double-stranded DNA, consisting of 155,527 bp, contained a pair of inverted repeat regions (IRa and IRb) of 25,187 bp each, which were separated by small and large single copy regions of 86,...

journal_title：Plant cell reports

authors： Kim JS,Jung JD,Lee JA,Park HW,Oh KH,Jeong WJ,Choi DW,Liu JR,Cho KY

更新日期：2006-04-01 00:00:00

abstract：:To develop an alternative genetic transformation system that is not dependent on an antibiotic selection strategy, the phosphomannose isomerase gene (pmi) system was evaluated for producing transgenic plants of chickpea (Cicer arietinum L.). A shoot morphogenesis protocol based on the thidiazuron (TDZ)-induced shoot m...

journal_title：Plant cell reports

authors： Patil G,Deokar A,Jain PK,Thengane RJ,Srinivasan R

更新日期：2009-11-01 00:00:00

abstract：KEY MESSAGE:Phytophthora infestans resistant somatic hybrids of S. × michoacanum (+) S. tuberosum and autofused 4 x S. × michoacanum were obtained. Our material is promising to introgress resistance from S. × michoacanum into cultivated potato background. Solanum × michoacanum (Bitter.) Rydb. (mch) is a wild diploid (2...

journal_title：Plant cell reports

authors： Smyda P,Jakuczun H,Dębski K,Sliwka J,Thieme R,Nachtigall M,Wasilewicz-Flis I,Zimnoch-Guzowska E

更新日期：2013-08-01 00:00:00

abstract：KEY MESSAGE:An antigenic protein targeting two epitopes from the Zaire ebolavirus GP1 protein was expressed in plant cells rendering an antigen capable of inducing humoral responses in mouse when administered subcutaneously or orally. The 2014 Ebola outbreak made clear that new treatments and prophylactic strategies to...

journal_title：Plant cell reports

authors： Ríos-Huerta R,Monreal-Escalante E,Govea-Alonso DO,Angulo C,Rosales-Mendoza S

更新日期：2017-02-01 00:00:00