Abstract:
:Fusarium head blight (FHB), caused primarily by Fusarium graminearum, is a major disease problem in wheat (Triticum aestivum). Genetic engineering holds significant potential to enhance FHB resistance in wheat. Due to the requirement of screening for FHB resistance on flowers at anthesis, the number of screens carried out in a year is limited. Our objective was to evaluate the feasibility of using the rapid-maturing dwarf wheat cultivar Apogee as an alternative genotype for transgenic FHB resistance research. Our transformation efficiency (number of transgenic plants/number of embryos) for Apogee was 1.33%. Apogee was also found to exhibit high FHB susceptibility and reached anthesis within 4 weeks. Interestingly, microsatellite marker haplotype analysis of the chromosome 3BS FHB resistant quantitative trait locus (QTL) region indicated that this region maybe deleted in Apogee. Our results indicate that Apogee is particularly well suited for accelerating transgenic FHB resistance research and transgenic wheat research in general.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Mackintosh CA,Garvin DF,Radmer LE,Heinen SJ,Muehlbauer GJdoi
10.1007/s00299-005-0059-4keywords:
subject
Has Abstractpub_date
2006-04-01 00:00:00pages
313-9issue
4eissn
0721-7714issn
1432-203Xjournal_volume
25pub_type
杂志文章abstract::Pigeon pea [Cajanus cajan (L.) Millsp.] (Family: Fabaceae) is an important legume crop cultivated across 50 countries in Asia, Africa, and the Americas; and ranks fifth in area among pulses after soybean, common bean, peanut, and chickpea. It is consumed as a major source of protein (21%) to the human population in ma...
journal_title:Plant cell reports
pub_type: 杂志文章,评审
doi:10.1007/s00299-010-0899-4
更新日期:2010-10-01 00:00:00
abstract::The influence of light on the expression of NADPH: protochlorophyllide oxidoreductase has been studied in different plant species. The presumptive precursors to this enzyme have been characterized by in vitro translation of poly (A) RNA and immunoprecipitation. Two bands of apparent molecular weights of about 42 000 a...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269229
更新日期:1983-02-01 00:00:00
abstract::Gastrodia elata Bl. is an achlorophyllous orchid plant feeding on the fungus Armillaria mellea. The plant lives underground during its life cycle except for florescence. Gastrodianins, members of the superfamily of monocot mannose-binding protein (MBP), have been identified from Gastrodia elata, yet their physiologica...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-006-0226-2
更新日期:2007-03-01 00:00:00
abstract::Autoclaving is a standard procedure for sterilizing nutrient media for plant tissue cultures. Most tissue cultures are grown at pH 5.2 to 5.8 with pH adjustments being made prior to autoclaving. This paper reports that there are significant differences between initial pH levels and pH levels following autoclaving, par...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269825
更新日期:1986-08-01 00:00:00
abstract:KEY MESSAGE:By comparing series full-length cDNA libraries stressed and control, the dynamic process of salt stress response in Upland cotton was studied, and reactive oxygen species and gibberellins signaling pathways were proposed. The Upland cotton is the most important fiber plant with highly salt tolerance. Howeve...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-015-1761-5
更新日期:2015-06-01 00:00:00
abstract::It has been reported that long term cell cultures from maize endosperms are not completely de-differentiated, maintaining some tissue-specific synthesis. We analyzed the expression of zein (the major storage protein of maize seeds) in cultures derived from wildtype and opaque-2 maize endosperms. In wildtype cultures, ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00272049
更新日期:1989-03-01 00:00:00
abstract::A procedure has been developed for the clonal propagation of Picrorhiza kurroa Royle ex Benth. through shoot tip culture. Murashige and Skoog's medium (1962) supplemented with kinetin (3.0 to 5.0 mg/l) supported rapid proliferation of multiple shoots from the explants. Addition of indole-3-acetic acid (1.0 mg/l) to th...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269324
更新日期:1988-05-01 00:00:00
abstract::The hybridity of eleven somatic hybrids between a diploidS. tuberosum and a diploidS. phureja clone could be verified because the parent karyotypes differed in their C-banding patterns. The hybrids were hypotetraploid and some carried structurally rearranged chromosomes and/or minute centric fragments. The nucleolar c...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00716844
更新日期:1989-02-01 00:00:00
abstract:KEY MESSAGE:Differentiation level but not transgene copy number influenced activation of a gene containment system in poplar. Heat treatments promoted CRE gene body methylation. The flower-specific transgene deletion was confirmed. Gene flow between genetic modified trees and their wild relatives is still motive of con...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-015-1890-x
更新日期:2016-02-01 00:00:00
abstract::Diuraphis noxia (Russian wheat aphid, RWA) is a major pest on wheat in South Africa and most other wheat growing countries. Being a probing-sucking insect, RWAs insert their stylets into the phloem sieve elements and feed on the phloem sap. This feeding causes necrotic lesions in resistant varieties, or decoloration o...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-005-0001-9
更新日期:2006-02-01 00:00:00
abstract::Human growth hormone (hGH), a pituitary-derived polypeptide, evidences a wide range of biological functions, including protein synthesis, cell proliferation, and metabolism. A synthetic hGH gene (shGH) has been synthesized on the basis of plant-optimized codon usage via an overlap PCR strategy and located in a plant e...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-008-0514-0
更新日期:2008-05-01 00:00:00
abstract::DNA fingerprints generated by the Jeffreys' probes, 33.6 and 33.15, indicated the presence of minisatellite-like sequences in the red clover genome. The fingerprints generated by probe 33.6 gave less background and fewer but better defined bands than those obtained with probe 33.15. Assay of a regenerative somaclonal ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00235293
更新日期:1993-12-01 00:00:00
abstract::A cDNA clone encoding an ADP-ribosylation factor from potato (Solanum tuberosum L.) was isolated. The nucleotide and deduced amino acid sequences show high homology to known ADP-ribosylation factor sequences from Arabidopsis, yeast, cow and man. In northern blot experiments, all tissues analysed showed expression of t...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233786
更新日期:1994-12-01 00:00:00
abstract:: Mature ovules of Dianthus (Caryophyllaceae) were histologically observed by clearing and serial sectioning to characterize the cells of the embryo sac. The results show that the mature embryo sac was located deep inside the hemitropous ovule due to thick nucellar tissue at the micropylar region. For the isolation of ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050753
更新日期:2000-04-01 00:00:00
abstract::To develop an alternative genetic transformation system that is not dependent on an antibiotic selection strategy, the phosphomannose isomerase gene (pmi) system was evaluated for producing transgenic plants of chickpea (Cicer arietinum L.). A shoot morphogenesis protocol based on the thidiazuron (TDZ)-induced shoot m...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-009-0766-3
更新日期:2009-11-01 00:00:00
abstract::Hypocotyl explants of Albizzia julibrissin were placed on Gamborg's B5 medium supplemented with various levels of paclobutrazol, uniconazole, prohexadione calcium, or GA3. Callus formation was evident within one week after placement of the explants on the culture media. Green nodule-like structures protruded from the ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00235303
更新日期:1993-12-01 00:00:00
abstract::This study aimed at establishing a protocol to increase the number of regenerated shoots and to limit the recovery of "escapes" during the regeneration of transgenic flax plants (cv Barbara). Here, we describe how light, adapted media and selection scheme could stimulate the transformation process, the organogenic pot...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-008-0638-2
更新日期:2009-03-01 00:00:00
abstract::We have previously developed procedures for the efficient production of sweet orange (Citrus sinensis L. Osbeck) and Carrizo citrange (C. sinensis L. Osbeck×Poncirus trifoliata L. Raf.) transgenic plants using an Agrobacterium tumefaciens-mediated transformation and shoot tip grafting in vitro regeneration system. We ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050311
更新日期:1997-09-01 00:00:00
abstract::Cotyledons from aseptically grown seedlings of sainfoin (Onobrychis viciifolia Scop.) were used as explant material to grow callus tissue for periods of 21 and 31 days. The formation of cells containing condensed tannins was induced by adding a range of BAP concentrations to an mB5 culture medium containing 2,4-D. Aft...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269314
更新日期:1988-05-01 00:00:00
abstract::Factors involved in promoting caulogenesis from hypocotyl explants of Phyllanthus fraternus were studied. Hypocotyl explants were cultured on B5 medium supplemented with 2,4-D or NAA in the presence and absence of BAP (at concentrations 0, 10(-7), 10(-6) and 10(-5)M). Adventitious shoots differentiated from callus dev...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232932
更新日期:1994-08-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-007-0321-z
更新日期:2007-07-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF01275506
更新日期:1997-06-01 00:00:00
abstract::Protoplasts derived from oat (Avena sativa L.) suspension culture cells (7 days after subculturing) were electroporated with plasmid DNA containing the Escherichia coli uidA gene encoding the ß-glucuronidase reporter enzyme. Consistently high enzyme activity was observed with electroporation conditions of 500 μF and 1...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00234710
更新日期:1993-05-01 00:00:00
abstract::The effects of cytokinins on the different branches of the indole alkaloid pathway were investigated in Catharanthus roseus cell cultures. Addition of zeatin to a 2,4-dichlorophenoxyacetic acid-containing medium decreased tryptamine levels and increased the bioconversion of secologanin to ajmalicine. Zeatin also enhan...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233425
更新日期:1993-10-01 00:00:00
abstract::Chrysosplenium americanum (Saxifragaceae) accumulates a variety of partially O-methylated flavonol glucosides. Because of the semi-aquatic nature of this plant and its extensive contamination with endogenous organisms, the initiation of shoot and callus cultures could only be achieved after (a) using a special surface...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00270122
更新日期:1988-03-01 00:00:00
abstract::Compact, friable and embryogenic calli were initiated from immature inflorescences and young leaf bases of one week old seedlings of Paspalum scrobiculatum cultured on MS medium supplemented with 2,4-D. A stable, embryogenic suspension culture was initiated from these calli and maintained in a liquid version of the sa...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00274134
更新日期:1989-05-01 00:00:00
abstract::In vitro clonal propagation of plants should generate identical copies of the selected genotype. However, associated stress might result in a breakdown of control mechanisms and consequent instability of the genome. We have used several molecular methods to assess the genetic stability of long-term propagated (24 year...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-007-0413-9
更新日期:2007-11-01 00:00:00
abstract:KEY MESSAGE:Sugarcane lines transformed with an alanine aminotransferase gene demonstrated an improved nitrogen use efficiency compared with untransformed controls in a pot trial under low nitrogen conditions. ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-015-1768-y
更新日期:2015-05-01 00:00:00
abstract::Responses induced by Cd exposure were assessed in tobacco seedlings (Nicotiana tabacum L.) using macro and molecular indices. The 100 microM of Cd exposure reduced the total dry weight and chlorophyll index of the seedlings as much as the genuine Fe-deficiency. Concentration of Fe in the shoots decreased, whereas that...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-005-0092-3
更新日期:2006-04-01 00:00:00
abstract::Callus derived from stem segments of loblolly pine (Pinus taeda L.) was subcultured to media containing 0.5% of various mono-, di-, tri- or polysaccharides. None of the carbohydrate sources tested were superior to sucrose. Growth on twelve of the 20 carbohydrates tested was more than 75% of that obtained for sucrose; ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00276676
更新日期:1987-04-01 00:00:00