Level of tissue differentiation influences the activation of a heat-inducible flower-specific system for genetic containment in poplar (Populus tremula L.).


KEY MESSAGE:Differentiation level but not transgene copy number influenced activation of a gene containment system in poplar. Heat treatments promoted CRE gene body methylation. The flower-specific transgene deletion was confirmed. Gene flow between genetic modified trees and their wild relatives is still motive of concern. Therefore, approaches for gene containment are required. In this study, we designed a novel strategy for achieving an inducible and flower-specific transgene removal from poplar trees but still expressing the transgene in the plant body. Hence, pollen carrying transgenes could be used for breeding purposes under controlled conditions in a first phase, and in the second phase genetic modified poplars developing transgene-free pollen grains could be released. This approach is based on the recombination systems CRE/loxP and FLP/frt. Both gene constructs contained a heat-inducible CRE/loxP-based spacer sequence for in vivo assembling of the flower-specific FLP/frt system. This allowed inducible activation of gene containment. The FLP/frt system was under the regulation of a flower-specific promoter, either CGPDHC or PTD. Our results confirmed complete CRE/loxP-based in vivo assembling of the flower-specific transgene excision system after heat treatment in all cells for up to 30 % of regenerants derived from undifferentiated tissue cultures. Degradation of HSP::CRE/loxP spacer after recombination but also persistence as extrachromosomal DNA circles were detected in sub-lines obtained after heat treatments. Furthermore, heat treatment promoted methylation of the CRE gene body. A lower methylation level was detected at CpG sites in transgenic sub-lines showing complete CRE/loxP recombination and persistence of CRE/loxP spacer, compared to sub-lines with incomplete recombination. However, our results suggest that low methylation might be necessary but not sufficient for recombination. The flower-specific FLP/frt-based transgene deletion was confirmed in 6.3 % of flowers.


Plant Cell Rep


Plant cell reports


Hoenicka H,Lehnhardt D,Nunna S,Reinhardt R,Jeltsch A,Briones V,Fladung M




Has Abstract


2016-02-01 00:00:00














  • Functional characterization of a chalcone synthase from the liverwort Plagiochasma appendiculatum.

    abstract:KEY MESSAGE:A chalcone synthase gene ( PaCHS ) was isolated and functionally characterized from liverwort. The ectopic expression of PaCHS in Marchantia paleacea callus raised the flavonoids content. Chalcone synthase (CHS; EC is pivotal for the biosynthesis of flavonoid and anthocyanin pigments in plants. It...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Yu HN,Wang L,Sun B,Gao S,Cheng AX,Lou HX

    更新日期:2015-02-01 00:00:00

  • Effect of cytokinin on morphological changes of suspension cultured cells of the moss, Barbula unguiculata.

    abstract::Suspension cultured cells of the moss, Barbula unguiculata, grow actively in both light and dark culture. Light-grown cells contain chlorophyll and exhibit an undifferentiated callus form. When cells are transferred to a dark condition, they develop into protonemata. Protonemata formation in the dark can be inhibited ...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Takio S

    更新日期:1989-03-01 00:00:00

  • Anthocyanin accumulation and changes in activities of phenylalanine ammonia-lyase and chalcone synthase in roselle (Hibiscus sabdariffa L.) callus cultures.

    abstract::Time-course changes in anthocyanin accumulation, phenylalanine ammonia-lyase activity and chalcone synthase activity were examined in roselle callus tissues incubated under different culture conditions. Phenylalanine ammonia-lyase activity was not affected by either the kind of auxin supplemented to the medium or ligh...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Mizukami H,Tomita K,Ohashi H

    更新日期:1989-12-01 00:00:00

  • Growth inhibition by exogenous proline and its metabolism in saltgrass (Distichlis spicata) suspension cultures.

    abstract::The growth of Distichlis spicata suspension cultures in LS medium without NaCl was inhibited 54% by 2 mM proline. In medium containing 260 mM NaCl, 10 mM proline inhibited growth by only 22%. The uptake and metabolism of 10 mM L-[1-(13)C] proline was followed by (13)C NMR and ninhydrin analyses of suspensions cultured...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Rodriguez MM,Heyser JW

    更新日期:1988-08-01 00:00:00

  • Organic nitrogen stimulates caulogenesis from hypocotyl callus of Phyllanthus fraternus.

    abstract::Factors involved in promoting caulogenesis from hypocotyl explants of Phyllanthus fraternus were studied. Hypocotyl explants were cultured on B5 medium supplemented with 2,4-D or NAA in the presence and absence of BAP (at concentrations 0, 10(-7), 10(-6) and 10(-5)M). Adventitious shoots differentiated from callus dev...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Rajasubramaniam S,Pardha Saradhi P

    更新日期:1994-08-01 00:00:00

  • Interspecific somatic hybrids between Solanum khasianum and S. aculeatissimum produced by electrofusion.

    abstract::Mesophyll derived protoplasts of Solanum khasianum Clarke and S. aculeatissimum Jacq. were fused by electrofusion and resulted in interspecific hybrids. In total, 249 calluses were obtained from which more than 65% produced shoots. From the regenerated plantlets 45% could be identified as hybrids by chromosome counts ...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Stattmann M,Gerick E,Wenzel G

    更新日期:1994-01-01 00:00:00

  • Isolation and characterization of a novel cadmium-regulated Yellow Stripe-Like transporter (SnYSL3) in Solanum nigrum.

    abstract:KEY MESSAGE:SnYSL3 encodes a plasma-localized transporter delivering various metal-nicotianamine complexes. The expression of SnYSL3 is up-regulated by excess Cd, suggesting an important role for SnYSL3 in response to Cd stress. The Yellow Stripe-Like (YSL) transporters have been proposed to participate in metal uptake...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Feng S,Tan J,Zhang Y,Liang S,Xiang S,Wang H,Chai T

    更新日期:2017-02-01 00:00:00

  • Comprehensive selection of reference genes for quantitative gene expression analysis during seed development in Brassica napus.

    abstract:KEY MESSAGE:MicroRNAs have higher expression stability than protein-coding genes in B. napus seeds and are therefore good reference genes for miRNA and mRNA RT-qPCR analysis. Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) has become the "gold standard" to gain insight into function of ...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Machado RD,Christoff AP,Loss-Morais G,Margis-Pinheiro M,Margis R,Körbes AP

    更新日期:2015-07-01 00:00:00

  • Embryogenesis in suspension cultures of Datura innoxia Mill.

    abstract::In vitro plant regeneration via embryogenesis was obtained in suspension cultures of Datura innoxia Mill. Embryogenesis was induced in suspension cultures raised from callus of androgenetic origin, using LS liquid medium supplemented with 0.22 mg/l 2,4-D. The total number of embryos formed was variable over time in cu...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Sharma VK,Jethwani V,Kothari SL

    更新日期:1993-08-01 00:00:00

  • Inefficient expression of the DNA-binding domain of GAL4 in transgenic plants.

    abstract::The DNA-binding domain of the yeast transcriptional activator GAL4 was expressed in transgenic tobacco plants in order to attempt to obtain specific repression of reporter genes. Expression of the GUS and the NPTII gene was essentially the same regardless of the presence of a gene encoding a functional GAL4 DNA-bindin...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Reichel C,Feltkamp D,Walden R,Steinbiß HH,Schell J,Rosahl S

    更新日期:1995-10-01 00:00:00

  • The rice MAPKK-MAPK interactome: the biological significance of MAPK components in hormone signal transduction.

    abstract::Mitogen-activated protein kinase (MAPK) signaling cascades are evolutionarily conserved fundamental signal transduction pathways. A MAPK cascade consists of many distinct MAPKKK-MAPKK-MAPK modules linked to various upstream receptors and downstream targets through sequential phosphorylation and activation of the casca...

    journal_title:Plant cell reports

    pub_type: 杂志文章,评审


    authors: Singh R,Jwa NS

    更新日期:2013-06-01 00:00:00

  • Expression of H3N2 nucleoprotein in maize seeds and immunogenicity in mice.

    abstract:KEY MESSAGE:Oral administration of maize-expressed H3N2 nucleoprotein induced antibody responses in mice showing the immunogenicity of plant-derived antigen and its potential to be utilized as a universal flu vaccine. Influenza A viruses cause influenza epidemics that are devastating to humans and livestock. The vaccin...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Nahampun HN,Bosworth B,Cunnick J,Mogler M,Wang K

    更新日期:2015-06-01 00:00:00

  • Deciphering the physiological and molecular mechanisms for copper tolerance in autotetraploid Arabidopsis.

    abstract:KEY MESSAGE:Autotetraploid Arabidopsis line esd and 4COL exhibit enhanced tolerance to Cu stress by enhancing activation of antioxidative defenses, altering expression of genes related to Cu transport, chelation, and ABA-responsive. Autopolyploidy is ubiquitous among angiosperms and often results in better adaptation t...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Li M,Xu G,Xia X,Wang M,Yin X,Zhang B,Zhang X,Cui Y

    更新日期:2017-10-01 00:00:00

  • Extracellular accumulation of high specific-activity peroxidase by cell suspension cultures of cowpea.

    abstract::Cell suspension cultures of cowpea (Vigna sp.) were able to produce extracellular peroxidase. Different growth regulator concentrations induced different peroxidase activity in callus. The crude extracellular medium after four weeks of culture showed higher (6 times) specific peroxidase activity and higher thermo stab...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Moreno OA,Vazquez-Duhalt R,Nolasco H

    更新日期:1990-07-01 00:00:00

  • Experiments on tissue culture in the genus Lycopersicon miller : Shoot formation from protoplasts of tomato long-term cell cultures.

    abstract::Callus cultures from cotyledon explants were established and maintained in culture for more than two years. After several months callus cultures were transferred into liquid medium and cultured as cell suspensions. Protoplasts were isolated from these cell suspension cultures and cultured in a liquid medium. After for...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Koblitz H,Koblitz D

    更新日期:1982-06-01 00:00:00

  • Effect of 1-Aminocyclopropane-1-carboxylic acid and aminoethoxyvinylglycine on ethylene emanation and somatic embryogenesis from orchardgrass leaf cultures.

    abstract::Ethylene emanation rates were assessed from leaf tissues of an embryogenic seed plant (Cycle 0) and regeneration cycle plants selected for enhanced embryogenesis (Cycles I, II and IV). In all experiments, ethylene was assessed from the basal 1 cm portion of the innermost leaf. Ethylene emanation was five-fold higher i...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Songstad DD,Petracek PD,Sams CE,Conger BV

    更新日期:1989-03-01 00:00:00

  • Development of a CAPS marker system for genotyping European pear cultivars harboring 17 S alleles.

    abstract::The full-length cDNAs of eight S ribonucleases (S-RNases) were cloned from stylar RNA of European pear cultivars that could not be characterized by the cleaved amplified polymorphic sequences (CAPS) marker system for genotyping European pear cultivars harboring nine S alleles Sa, Sb, Sd, Se, Sh, Sk, Sl, Sq, and Sr. Co...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Moriya Y,Yamamoto K,Okada K,Iwanami H,Bessho H,Nakanishi T,Takasaki T

    更新日期:2007-03-01 00:00:00

  • Growth and rosmarinic acid production in cell suspension cultures of Salvia officinalis L.

    abstract::Rosmarinic acid (RA) is a natural antioxidant produced by cell suspension cultures of sage (Salvia officinalis L.). The growth and production of RA by these cells can be modified by the type of culture medium. Production can be increased 10-fold to attain 6.4 g.1(-1) under optimal conditions. Investigation of kinetics...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Hippolyte I,Marin B,Baccou JC,Jonard R

    更新日期:1992-04-01 00:00:00

  • Genetic relatedness of Portuguese almond cultivars assessed by RAPD and ISSR markers.

    abstract::Randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers were used to analyse the genetic diversity of Portuguese Prunus dulcis cultivars and their relationship to important foreign cultivars. Of the primers tested, 6 (out of 60) RAPD and 5 (out of 18) ISSR primers were selected for th...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Martins M,Tenreiro R,Oliveira MM

    更新日期:2003-08-01 00:00:00

  • Competitive interaction between two functional S-haplotypes confer self-compatibility on tetraploid Chinese cherry (Prunus pseudocerasus Lindl. CV. Nanjing Chuisi).

    abstract::Self-incompatibility (SI) has been studied extensively at the molecular level in Solanaceae, Rosaceae and Scrophulariaceae, all of which exhibit gametophytic self-incompatibility (GSI). In the present study, four PpsS-haplotypes (Prunus pseudocerasus S-haplotypes) comprising at least two genes, i.e., PpsS-RNase (P. ps...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Huang SX,Wu HQ,Li YR,Wu J,Zhang SJ,Heng W,Zhang SL

    更新日期:2008-06-01 00:00:00

  • Plant lipid environment and membrane enzymes: the case of the plasma membrane H+-ATPase.

    abstract::Several lipid classes constitute the universal matrix of the biological membranes. With their amphipathic nature, lipids not only build the continuous barrier that confers identity to every cell and organelle, but they are also active actors that modulate the activity of the proteins immersed in the lipid bilayer. The...

    journal_title:Plant cell reports

    pub_type: 杂志文章,评审


    authors: Morales-Cedillo F,González-Solís A,Gutiérrez-Angoa L,Cano-Ramírez DL,Gavilanes-Ruiz M

    更新日期:2015-04-01 00:00:00

  • Phytochelatin accumulation and stress tolerance in tomato cells exposed to cadmium.

    abstract::Cell suspension cultures of tomato (Lycopersicon esculentum) adapted to growing continuously in the presence of 0.1 mM CdCl2 and accumulated phytochelatins (PCs, poly(γ-Glu-Cys)n-Gly). The highest level of PCs was measured 4 days after inoculation and coincided with the peak of cellular cadmium concentration. At this ...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Gupta SC,Goldsbrough PB

    更新日期:1990-12-01 00:00:00

  • Long-term optimized embryogenic cultures in durum wheat (Triticum durum Desf.).

    abstract::Five varieties of durum wheat: Appulo, Ofanto, Latino, Creso, and Castello (Triticum durum Desf.) adapted to the semi-arid mediterranean environment have been tested for their in vitro response. Compact, embryogenic, highly regenerable calli originated from primary callus derived through proliferation of the scutellum...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Borrelli GM,Lupotto E,Locatelli F,Wittmer G

    更新日期:1991-09-01 00:00:00

  • Production of gynogenic haploids of Hordeum vulgare L.

    abstract::Haploid plants were regenerated from cultured unfertilized ovaries of Hordeum vulgare L. (barley). Optimal response was obtained by the addition of 0.6 μM 4-chloro-2-methylphenoxyacetic acid (MCPA), 2.8 μM indole-3-acetic acid (IAA) and 4.4 μM 6-benzyladenine (BA) in the N6 medium. Further increase in the rate of call...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Castillo AM,Cistué L

    更新日期:1993-01-01 00:00:00

  • Genetic transformation of lime (Citrus aurantifolia Swing.): factors affecting transformation and regeneration.

    abstract::We have previously developed procedures for the efficient production of sweet orange (Citrus sinensis L. Osbeck) and Carrizo citrange (C. sinensis L. Osbeck×Poncirus trifoliata L. Raf.) transgenic plants using an Agrobacterium tumefaciens-mediated transformation and shoot tip grafting in vitro regeneration system. We ...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Peña L,Cervera M,Juárez J,Navarro A,Pina JA,Navarro L

    更新日期:1997-09-01 00:00:00

  • Generation and analysis of expressed sequence tags from a normalized cDNA library of young leaf from Ma bamboo (Dendrocalamus latiflorus Munro).

    abstract::Ma bamboo (Dendrocalamus latiflorus Munro) belongs to Dendrocalamus genus, Bambusease tribe, Bambusoideae subfamily, Poaceae family. It is a representative species of clumping bamboo, and a principal commercial species for various construction purposes using mature culms and for human consumption using young shoots. A...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Gao ZM,Li CL,Peng ZH

    更新日期:2011-11-01 00:00:00

  • Crown gall transformation of lentil (Lens culinaris Medik.) with virulent strains of Agrobacterium tumefaciens.

    abstract::Four diverse strains of Agrobacterium tumefaciens (C58, Ach5, GV3111, and A281) were capable of inducing tumors at a high frequency on inoculated stems of lentil (Lens culinaris Medik. cultivar Laird) in vivo, and on excised shoot apices in vitro. GV3111 and Ach5 produced the largest and heaviest tumors in vivo, while...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Warkentin TD,McHughen A

    更新日期:1991-12-01 00:00:00

  • Callus formation from protoplasts of a sugarbeet cell suspension culture.

    abstract::Protoplasts of sugarbeet (Beta vulgaris L.) were isolated from cell suspension cultures and cultured in modified PGo medium. Conditions required for the efficient division of the protoplasts were investigated.The optimal combination of phytohormones was found to be 1 mg/l NAA, 0.2 mg/l 2,4-D, 0.5 mg/l zeatin. Protopla...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Szabados L,Gaggero C

    更新日期:1985-08-01 00:00:00

  • Opportunities and constraints to biotechnological applications in the Caribbean: transgenic papayas in Jamaica and Venezuela.

    abstract::In this opinion article, we briefly review the status of crop biotechnology research-with emphasis on the development of GM crops-in Jamaica and Venezuela. We focus on the transgenic papayas developed for both countries, and examine the factors hindering not only the development and application of this biotechnologica...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Fermin G,Tennant P

    更新日期:2011-05-01 00:00:00

  • ThPP1 gene, encodes an inorganic pyrophosphatase in Thellungiella halophila, enhanced the tolerance of the transgenic rice to alkali stress.

    abstract:KEY MESSAGE:An inorganic pyrophosphorylase gene, ThPP1 , modulated the accumulations of phosphate and osmolytes by up-regulating the differentially expression genes, thus enhancing the tolerance of the transgenic rice to alkali stress (AS). Inorganic pyrophosphorylase is essential in catalyzing the hydrolysis of pyroph...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: He R,Yu G,Han X,Han J,Li W,Wang B,Huang S,Cheng X

    更新日期:2017-12-01 00:00:00