Level of tissue differentiation influences the activation of a heat-inducible flower-specific system for genetic containment in poplar (Populus tremula L.).


KEY MESSAGE:Differentiation level but not transgene copy number influenced activation of a gene containment system in poplar. Heat treatments promoted CRE gene body methylation. The flower-specific transgene deletion was confirmed. Gene flow between genetic modified trees and their wild relatives is still motive of concern. Therefore, approaches for gene containment are required. In this study, we designed a novel strategy for achieving an inducible and flower-specific transgene removal from poplar trees but still expressing the transgene in the plant body. Hence, pollen carrying transgenes could be used for breeding purposes under controlled conditions in a first phase, and in the second phase genetic modified poplars developing transgene-free pollen grains could be released. This approach is based on the recombination systems CRE/loxP and FLP/frt. Both gene constructs contained a heat-inducible CRE/loxP-based spacer sequence for in vivo assembling of the flower-specific FLP/frt system. This allowed inducible activation of gene containment. The FLP/frt system was under the regulation of a flower-specific promoter, either CGPDHC or PTD. Our results confirmed complete CRE/loxP-based in vivo assembling of the flower-specific transgene excision system after heat treatment in all cells for up to 30 % of regenerants derived from undifferentiated tissue cultures. Degradation of HSP::CRE/loxP spacer after recombination but also persistence as extrachromosomal DNA circles were detected in sub-lines obtained after heat treatments. Furthermore, heat treatment promoted methylation of the CRE gene body. A lower methylation level was detected at CpG sites in transgenic sub-lines showing complete CRE/loxP recombination and persistence of CRE/loxP spacer, compared to sub-lines with incomplete recombination. However, our results suggest that low methylation might be necessary but not sufficient for recombination. The flower-specific FLP/frt-based transgene deletion was confirmed in 6.3 % of flowers.


Plant Cell Rep


Plant cell reports


Hoenicka H,Lehnhardt D,Nunna S,Reinhardt R,Jeltsch A,Briones V,Fladung M




Has Abstract


2016-02-01 00:00:00














  • Tobacco plants perturbed in the ubiquitin-dependent protein degradation system accumulate callose, salicylic acid, and pathogenesis-related protein 1.

    abstract::Transgenic tobacco plants expressing an inhibitor of ubiquitin-dependent protein degradation, ubiquitin variant ubR48, spontaneously formed necrotic lesions and displayed altered responsiveness to tobacco mosaic virus attack. These plants were analyzed for the accumulation of defense-related compounds and the expressi...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Conrath U,Klessig DF,Bachmair A

    更新日期:1998-08-01 00:00:00

  • Development of a phosphomannose isomerase-based Agrobacterium-mediated transformation system for chickpea (Cicer arietinum L.).

    abstract::To develop an alternative genetic transformation system that is not dependent on an antibiotic selection strategy, the phosphomannose isomerase gene (pmi) system was evaluated for producing transgenic plants of chickpea (Cicer arietinum L.). A shoot morphogenesis protocol based on the thidiazuron (TDZ)-induced shoot m...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Patil G,Deokar A,Jain PK,Thengane RJ,Srinivasan R

    更新日期:2009-11-01 00:00:00

  • Functional characterization of sugarcane MYB transcription factor gene promoter (PScMYBAS1) in response to abiotic stresses and hormones.

    abstract::The sugarcane (Saccharum officinarum) stress-related MYB transcription factor gene, ScMYBAS1, demonstrated induced response to water deficit and salt stress in our previous study. To elucidate its stress tolerance mechanism at the transcriptional level, we isolated and characterized the promoter (PScMYBAS1, 1,033 bp) ...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Prabu G,Prasad DT

    更新日期:2012-04-01 00:00:00

  • Low night temperature acclimation of Phalaenopsis.

    abstract::The capability of Phalaenopsis to acclimate its photosynthetic capacity and metabolic activity to cool night temperature conditions is crucial for improving orchid production in terms of efficient greenhouse heating. The extent to which Phalaenopsis possesses acclimation potential and the mechanistic background of the...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Pollet B,Vanhaecke L,Dambre P,Lootens P,Steppe K

    更新日期:2011-06-01 00:00:00

  • Polyamines and crown gall tumor growth.

    abstract::The effect of the polyamine spermidine on the growth of crown gall tumors was determined using the potato disc bioassay. Addition of lmM spermidine resulted in a 30-50% increase in tumor growth. The spermidine effect was found to be biphasic, with lmM being optimal. Closely related polyamines including spermine, as we...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Kulpa JM,Galsky AG,Lipetz P,Stephens R

    更新日期:1985-04-01 00:00:00

  • Tissue and protoplast culture studies in Lycopersicon esculentum miller var. flammatum lehm. cv. 'Bonner Beste' and its mutant chloronerva.

    abstract::Methods of shoot and plant development from cotyledon explants of the tomato cultivar 'Bonner Beste' and its mutant chloronerva are described. Cotyledon mesophyll protoplasts of the mutant chloronerva have been isolated and cultured, and induced to regenerate shoots and plants. By grafting regenerated shoots of the ch...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Koblitz H,Koblitz D

    更新日期:1983-08-01 00:00:00

  • An ethylene response factor (ERF5) promoting adaptation to drought and salt tolerance in tomato.

    abstract::A novel member of the AP2/ERF transcription factor family, SlERF5, was identified from a tomato mature leaf cDNA library screen. The complete DNA sequence of SlERF5 encodes a putative 244-amino acid DNA-binding protein which most likely acts as a transcriptional regulator and is a member of the ethylene responsive fac...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Pan Y,Seymour GB,Lu C,Hu Z,Chen X,Chen G

    更新日期:2012-02-01 00:00:00

  • Plant regeneration from leaf explants of Glycine clandestina Wendl.

    abstract::Fully fertile plants with the expected chromosome number 2n=40 were regenerated from excised leaf sections of Glycine clandestina. Shoots formed directly on the explants through organogenesis. Utilizing the same media and procedures fully fertile plants were also regenerated from cotyledon and hypocotyl tissue of the ...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Hymowitz T,Chalmers NL,Costanza SH,Saam MM

    更新日期:1986-06-01 00:00:00

  • Isolation, culture and callus regeneration of protoplasts of wild and cultivated Helianthus species.

    abstract::Protoplasts were isolated from seedling roots, hypocotyls, and cotyledons of four cultivars of Helianthus annuus and from leaves of axenic shoot cultures of the wild species H. praecox, H. scaberimus and H. rigidus. Optimal culture conditions were established for the respective protoplast systems, using the agarose be...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Bohorova NE,Cocking EC,Power JB

    更新日期:1986-08-01 00:00:00

  • Chromosome stability of cell suspension cultures of Nicotiana spp.

    abstract::Cell suspension cultures of Nicotiana were initiated using conditions designed to selectively favor stable chromosome number. These conditions included use of leaf explants to initiate cultures, growth of cells in culture medium containing 2,4-D, and transfer of cells with short subculture intervals. Four cell lines d...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Evans DA,Gamborg OL

    更新日期:1982-04-01 00:00:00

  • Cloning and characterization of HsfA2 from Lily (Lilium longiflorum).

    abstract::Heat shock transcription factors (Hsfs) are the terminal components of the signal transduction chain mediating the activation of genes responsive to both heat stress and a large number of chemical stressors. This paper aims to clone Hsf from lily and characterize its function by analyses of mRNA expression, transactiv...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Xin H,Zhang H,Chen L,Li X,Lian Q,Yuan X,Hu X,Cao L,He X,Yi M

    更新日期:2010-08-01 00:00:00

  • Vascular-specific activity of the Arabidopsis carotenoid cleavage dioxygenase 7 gene promoter.

    abstract::Carotenoid cleavage dioxygenases (CCDs) are involved in the production of diverse apocarotenoids including phytohormones, the visual molecules and the aromatic volatile compounds derived from carotenoids. Here, we examined the spatial expression of four of the CCD genes (AtCcd1, 4, 7 and 8) among the nine members of t...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Liang YS,Jeon YA,Lim SH,Kim JK,Lee JY,Kim YM,Lee YH,Ha SH

    更新日期:2011-06-01 00:00:00

  • Plant regeneration from different explants in Helianthus smithii Heiser.

    abstract::Leaf, rhizome, and stem explants of Helianthus smithii, a wild relative of the cultivated sunflower, have been tested for their morphogenic potential on media containing a range of hormonal combinations including α-naphthaleneacetic acid and 6-benzylaminopurine (0-2 mg/l). Somatic embryos appeared on the majority of t...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Laparra H,Stoeva P,Ivanov P,Hahne G

    更新日期:1997-07-01 00:00:00

  • Control of foreign polypeptide localization in specific layers of protein body type I in rice seed.

    abstract:KEY MESSAGE:Prolamin-GFP fusion proteins, expressed under the control of native prolamin promoters, were localized in specific layers of PB-Is. Prolamin-GFP fusion proteins were gradually digested from outside by pepsin digestion. In rice seed endosperm, protein body type I (PB-I) has a layered structure consisting of ...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Sasou A,Shigemitsu T,Saito Y,Tanaka M,Morita S,Masumura T

    更新日期:2016-06-01 00:00:00

  • Functional characterization of a stilbene synthase gene using a transient expression system in planta.

    abstract::The expression and functionality of a resveratrol synthase (RS) gene from peanut (Arachis hypogaea) was studied using an Agrobacterium tumefaciens-mediated transient expression system in Nicotiana benthamiana leaves. Functional analysis of RS was demonstrated by tracking its expression during 96 h. To measure the tran...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Condori J,Medrano G,Sivakumar G,Nair V,Cramer C,Medina-Bolivar F

    更新日期:2009-04-01 00:00:00

  • Mitochondria/nuclear signaling of alternative oxidase gene expression occurs through distinct pathways involving organic acids and reactive oxygen species.

    abstract::Cultured cells of tobacco (Nicotiana tabacum L. cv Petit Havana) were used to investigate signals regulating the expression of the "model" nuclear gene encoding the alternative oxidase (AOX) (AOX1), the terminal oxidase of the mitochondrial alternative respiratory pathway. Several conditions shown to induce AOX1 mRNA ...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Gray GR,Maxwell DP,Villarimo AR,McIntosh L

    更新日期:2004-12-01 00:00:00

  • The cation-efflux transporter BjCET2 mediates zinc and cadmium accumulation in Brassica juncea L. leaves.

    abstract::Brassica juncea L. is a Zn/Cd accumulator. To determine the physiological basis of its metal accumulation phenotype, the functional properties and role of the metal efflux transporter BjCET2 were investigated using transgenic technology. Heterologous expression of BjCET2 in the double mutant yeast strain Deltazrc1Delt...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Xu J,Chai T,Zhang Y,Lang M,Han L

    更新日期:2009-08-01 00:00:00

  • Genotypic and developmental regulation of transient expression of a reporter gene in soybean zygotic cotyledons.

    abstract::Processes involved in transformation of regenerable soybean (Glycine max (L.) Merrill) immature zygotic cotyledons were studied by assaying the transient expression of the β-glucuronidase gene driven by the 35S promoter and terminated at the 3' end by the soybean 7S storage protein gene. The plasmid containing the chi...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Moore PJ,Moore AJ,Collins GB

    更新日期:1994-07-01 00:00:00

  • β-glucuronidase gene expression and mRNA stability in oat protoplasts.

    abstract::Protoplasts derived from oat (Avena sativa L.) suspension culture cells (7 days after subculturing) were electroporated with plasmid DNA containing the Escherichia coli uidA gene encoding the ß-glucuronidase reporter enzyme. Consistently high enzyme activity was observed with electroporation conditions of 500 μF and 1...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Higgs DC,Colbert JT

    更新日期:1993-05-01 00:00:00

  • Use of stem proteins and isozymes for the identification of celery varieties.

    abstract::Separation of stem proteins by sodium dodecyl sulfate polyacrylamide gel electrophoresis permitted classification of 17 celery varieties (Apium graveolens L.) into 7 groups. Several of these groups could be further subdivided into 11 subgroups with gel electrophoresis patterns of 4 isozyme systems. Classification from...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Quiros CF,McGrath M,Stites JL

    更新日期:1987-04-01 00:00:00

  • Comparative analysis of cell cycle events in zygotic and somatic embryos of Cyclamen persicum indicates strong resemblance of somatic embryos to recalcitrant seeds.

    abstract::Embryo development and germination of Cyclamen persicum have been comparatively characterized for zygotic and somatic embryos with regard to mitotic activity and morphology in order to identify developmental abnormalities in somatic embryogenesis. Zygotic embryo development proved to be highly synchronous with distinc...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Schmidt T,Ewald A,Seyring M,Hohe A

    更新日期:2006-07-01 00:00:00

  • Somatic hybrids obtained by fusion betweenPoncirus trifoliata (2x) andFortunella hindsii (4x) protoplasts.

    abstract::Somatic hybrids were obtained by the symmetric fusion of embryogenic callus cells from tetraploid 'Mame' kumquat [Fortunella hindsii (Champ.) Swing.] and mesophyll cells from diploid trifoliate orange [Poncirus trifoliata (L.) Raf.]. Southern blot analysis of three regenerants revealed that they carried specific rDNA ...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Miranda M,Motomura T,Ikeda F,Ohgawara T,Saito W,Endo T,Omura M,Moriguchi T

    更新日期:1997-03-01 00:00:00

  • Competitive interaction between two functional S-haplotypes confer self-compatibility on tetraploid Chinese cherry (Prunus pseudocerasus Lindl. CV. Nanjing Chuisi).

    abstract::Self-incompatibility (SI) has been studied extensively at the molecular level in Solanaceae, Rosaceae and Scrophulariaceae, all of which exhibit gametophytic self-incompatibility (GSI). In the present study, four PpsS-haplotypes (Prunus pseudocerasus S-haplotypes) comprising at least two genes, i.e., PpsS-RNase (P. ps...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Huang SX,Wu HQ,Li YR,Wu J,Zhang SJ,Heng W,Zhang SL

    更新日期:2008-06-01 00:00:00

  • Biotechnological advances in Lilium.

    abstract::Modern powerful techniques in plant biotechnology have been developed in lilies (Lilium spp., Liliaceae) to propagate, improve and make new phenotypes. Reliable in vitro culture methods are available to multiply lilies rapidly and shorten breeding programs. Lilium is also an ideal model plant to study in vitro pollina...

    journal_title:Plant cell reports

    pub_type: 杂志文章,评审


    authors: Bakhshaie M,Khosravi S,Azadi P,Bagheri H,van Tuyl JM

    更新日期:2016-09-01 00:00:00

  • Programmed-cell-death hallmarks in incompatible pollen and papillar stigma cells of Olea europaea L. under free pollination.

    abstract::Programmed cell death (PCD) is a process that occurs both in animals and in plants and is an essential element in developmental processes. Pollination is a key factor in fruit production and self-incompatibility is one of the main limiting factors of this process. PCD has recently been put forward as a possible cause ...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Serrano I,Pelliccione S,Olmedilla A

    更新日期:2010-06-01 00:00:00

  • Differences in pectic polysaccharides between carrot embryogenic and non-embryogenic calli.

    abstract::Cell walls and media were obtained from three kinds of carrot cell culture, namely, embryogenic callus (EC), non-embryogenic callus (NC) and somatic embryos (SE), and analyzed for their sugar content and sugar composition by electrophoresis and gas chromatography. EC formed large cell clusters while NC formed small cl...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Kikuchi A,Satoh S,Nakamura N,Fujii T

    更新日期:1995-02-01 00:00:00

  • Cryopreservation of sweet potato (Ipomoea batatas [L.] Lam.) shoot tips by vitrification.

    abstract::Vitrification is a technically simple method for cryopreserving plant germplasm, requiring only the application of suitable cryoprotectants and rapid cooling rates. Sweetpotato (Ipomoea batatas [L.] Lam.) shoot tips obtained from in vitro plants survived liquid nitrogen (-196°C) exposure following a vitrification-indu...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Towill LE,Jarret RL

    更新日期:1992-05-01 00:00:00

  • Isolation and viral infection of Capsicum leaf protoplasts.

    abstract::A protocol for protoplast isolation was developed and tested with five Capsicum genotypes representing two cultivated species, C. annuum and C. chinense. Key variables included growth conditions for source plants and the concentration of mannitol used as osmoticum. Protoplasts isolated from each of the genotypes becam...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Murphy JF,Kyle MM

    更新日期:1994-04-01 00:00:00

  • Purple-leaved Ficus lyrata plants produced by overexpressing a grapevine VvMybA1 gene.

    abstract:KEY MESSAGE:This study established an efficient method of regenerating plants of Ficus lyrata and producing purple-leaved F. lyrata plants through genetic transformation using a VvMybA1 gene of grapevine. ABSTRACT:Ficus lyrata, a species with unique violin- or guitar-shaped leaves, was regenerated from leaf-derived ca...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Zhao J,Li ZT,Chen J,Henny RJ,Gray DJ,Chen J

    更新日期:2013-11-01 00:00:00

  • Photosynthetically active suspension cultures of potato spindle tuber viroid infected tomato cells as tools for studying viroid - host cell interaction.

    abstract::Photosynthetically active callus and cell suspension cultures were established from uninfected Lycopersicon peruvianum plants and from uninfected and potato spindle tuber viroid (PSTVd) infected plants of Lycopersicon esculentum cv. Rutgers. Viroid infection was maintained in photoheterotrophic culture on media contai...

    journal_title:Plant cell reports

    pub_type: 杂志文章


    authors: Stöcker S,Guitton MC,Barth A,Mühlbach HP

    更新日期:1993-09-01 00:00:00