Cytological changes associated with alkaloid production in cultured cells of Coptis japonica and Thalictrum minus.

Abstract:

:Cell structures were compared between alkaloid-producing and non-producing cell cultures of Coptis japonica and Thalictrum minus by electron microscopic observation. In alkaloid-producing cells of C. japonica, prior to the onset of alkaloid synthesis, the vacuoles showed a greater volume than in non-producing cells. These were characterized by a number of large starch grains in the cytoplasm. Furthermore, alkaloid-producing cells contained stacks of rough endoplasmic reticulum. Comparison of different cell lines suggested that there might be a negative correlation between accumulation of alkaloids and starch. Similar cytological differences were observed with T. minus cell cultures that release berberine into the culture medium. Alkaloid producing cells were found to contain an abundance of cytoplasmic vesicles (0.5 - 1 υm in diameter).

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Yamamoto H,Nakagawa K,Fukui H,Tabata M

doi

10.1007/BF00269721

subject

Has Abstract

pub_date

1986-02-01 00:00:00

pages

65-8

issue

1

eissn

0721-7714

issn

1432-203X

journal_volume

5

pub_type

杂志文章
  • Self-compatibility in 'Cristobalina' sweet cherry is not associated with duplications or modified transcription levels of S-locus genes.

    abstract::Sweet cherry shows S-RNase-based gametophytic self-incompatibility, which prevents self- and cross-fertilization between genetically related individuals. The specificity of the self-incompatible reaction is determined by two genes located in the S-locus. These encode a pistil-expressed ribonuclease (S-RNase) that inhi...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-010-0857-1

    authors: Wünsch A,Tao R,Hormaza JI

    更新日期:2010-07-01 00:00:00

  • Stable endosperm cell suspension cultures from wild-type and opaque-2 maize.

    abstract::Stable cell suspension cultures have been established from immature endosperms of A69Y wild-type and opaque-2 maize (Zea mays L.). Cultured cells are capable of storage protein (zein) synthesis and accumulation throughout the growth period. Electrophoretic patterns of zeins show, for opaque-2 cells, the preferential i...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00232330

    authors: Manzocchi LA

    更新日期:1991-02-01 00:00:00

  • Production of shikonin derivatives by cell suspension cultures of Lithospermum erythrorhizon : II. A new Medium for the production of shikonin derivatives.

    abstract::An excellent new medium was developed for the production of shikonin derivatives by suspension cultures of Lithospermum erythrorhizon. We investigated the effects of all the components of White's medium on the production of these derivatives. Nitrate, phosphate, copper, sulfate and sucrose had especially marked effect...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00269273

    authors: Fujita Y,Hara Y,Suga C,Morimoto T

    更新日期:1981-12-01 00:00:00

  • Functional characterization of sugarcane MYB transcription factor gene promoter (PScMYBAS1) in response to abiotic stresses and hormones.

    abstract::The sugarcane (Saccharum officinarum) stress-related MYB transcription factor gene, ScMYBAS1, demonstrated induced response to water deficit and salt stress in our previous study. To elucidate its stress tolerance mechanism at the transcriptional level, we isolated and characterized the promoter (PScMYBAS1, 1,033 bp) ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-011-1183-y

    authors: Prabu G,Prasad DT

    更新日期:2012-04-01 00:00:00

  • Abscisic acid-regulated Glb1 transient expression in cultured maize P3377 cells.

    abstract::In a study of the 5'-flanking sequence of the Zea mays L. (maize) Glb1 gene in vitro, serial promoter deletions were generated and linked with the β-glucuronidase (GUS) reporter gene. The promoter deletion-GUS fusions were introduced into the maize P3377 cell line by particle bombardment. GUS assays indicated that tre...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s002990050459

    authors: Liu S,Kriz A,Duncan D,Widholm J

    更新日期:1998-05-01 00:00:00

  • Generation and analysis of expressed sequence tags from a normalized cDNA library of young leaf from Ma bamboo (Dendrocalamus latiflorus Munro).

    abstract::Ma bamboo (Dendrocalamus latiflorus Munro) belongs to Dendrocalamus genus, Bambusease tribe, Bambusoideae subfamily, Poaceae family. It is a representative species of clumping bamboo, and a principal commercial species for various construction purposes using mature culms and for human consumption using young shoots. A...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-011-1112-0

    authors: Gao ZM,Li CL,Peng ZH

    更新日期:2011-11-01 00:00:00

  • Physiological and transcriptomic responses of reproductive stage soybean to drought stress.

    abstract:KEY MESSAGE:The dynamic alterations of the physiological and molecular processes in reproductive stage soybean indicated the dramatic impact caused by drought. Drought is a major abiotic stress that limits soybean (Glycine max) production. Most prior studies were focused on either model species or crops that are at the...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-018-2332-3

    authors: Xu C,Xia C,Xia Z,Zhou X,Huang J,Huang Z,Liu Y,Jiang Y,Casteel S,Zhang C

    更新日期:2018-12-01 00:00:00

  • Cryopreservation of in vitro-grown axillary shoot-tip meristems of mint (Mentha spicata L.) by encapsulation vitrification.

    abstract:: Alginate-coated meristems from in vitro-grown axillary buds of mint (Mentha spicata L.) were successfully cryopreserved by vitrification. Excised meristems from nodal segments cold hardened at 4  °C for 3 weeks were encapsulated and osmoprotected by a mixture of 2 M glycerol plus 0.4 M sucrose. These meristems were d...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s002990050725

    authors: Hirai D,Sakai A

    更新日期:1999-12-01 00:00:00

  • Two EST-derived marker systems for cultivar identification in tree peony.

    abstract::Tree peony (Paeonia suffruticosa Andrews), a woody deciduous shrub, belongs to the section Moutan DC. in the genus of Paeonia of the Paeoniaceae family. To increase the efficiency of breeding, two EST-derived marker systems were developed based on a tree peony expressed sequence tag (EST) database. Using target region...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-011-1164-1

    authors: Zhang JJ,Shu QY,Liu ZA,Ren HX,Wang LS,De Keyser E

    更新日期:2012-02-01 00:00:00

  • An efficient adventitious shoot regeneration system for Zhanhua winter jujube (Zizyphus jujuba Mill.) using leaf explants.

    abstract::The direct induction of adventitious shoots from leaf explants obtained from adult plants of Zhanhua winter jujube, an elite variety of Zizyphus jujuba Mill., is reported. The percentage of leaf explants producing shoots and the average number of shoots per explant were significantly improved when 10-day-old leaves we...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-005-0920-5

    authors: Gu XF,Zhang JR

    更新日期:2005-03-01 00:00:00

  • Interspecific somatic hybrids between Solanum khasianum and S. aculeatissimum produced by electrofusion.

    abstract::Mesophyll derived protoplasts of Solanum khasianum Clarke and S. aculeatissimum Jacq. were fused by electrofusion and resulted in interspecific hybrids. In total, 249 calluses were obtained from which more than 65% produced shoots. From the regenerated plantlets 45% could be identified as hybrids by chromosome counts ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00239891

    authors: Stattmann M,Gerick E,Wenzel G

    更新日期:1994-01-01 00:00:00

  • Plant regeneration from protoplast-derived callus of rice (Oryza sativa L.).

    abstract::Protoplasts isolated from cultured rice cells of an A-58 cytoplasmic male sterile line (A-58 MS)(Oryza sativa L.) were used to investigate the regeneration of rice plants. A cultured cell line (T3) of A-58 MS with a high growth rate and dense cytoplasm was selected. About 10% of the protoplasts prepared from this esta...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00269240

    authors: Yamada Y,Yang ZQ,Tang DT

    更新日期:1986-04-01 00:00:00

  • Mechanism of exogenous cytokinins inducing bulbil formation in Lilium lancifolium in vitro.

    abstract:KEY MESSAGE:The cytokinin pathway promotes the initiation of bulbil formation, and iPA may an important type of cytokinin during bulbil formation in Lilium lancifolium. Bulbils are important vegetative reproductive organs in triploid Lilium lancifolium. We previously showed that cytokinins are involved in bulbil format...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-020-02535-x

    authors: He G,Yang P,Tang Y,Cao Y,Qi X,Xu L,Ming J

    更新日期:2020-07-01 00:00:00

  • Differences in pectic polysaccharides between carrot embryogenic and non-embryogenic calli.

    abstract::Cell walls and media were obtained from three kinds of carrot cell culture, namely, embryogenic callus (EC), non-embryogenic callus (NC) and somatic embryos (SE), and analyzed for their sugar content and sugar composition by electrophoresis and gas chromatography. EC formed large cell clusters while NC formed small cl...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00232028

    authors: Kikuchi A,Satoh S,Nakamura N,Fujii T

    更新日期:1995-02-01 00:00:00

  • Isolation, culture and plantlet regeneration from cotyledon and mesophyll protoplasts of two pickling cucumber (Cucumis sativus L.) genotypes.

    abstract::Optimal protoplast yields from cotyledons (2.0×10(6) protoplasts/ 0.5 g tissue) and from true leaves (5.0×10(6) protoplasts/g tissue) of two Cucumis sativus genotypes were obtained following a 16 h digestion with, respectively, 1.25% pectinase+0.5% Cellulysin and 0.5 % pectinase+ 1.0% Cellulysin. Enzyme solutions were...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00231549

    authors: Punja ZK,Tang FA,Sarmento GG

    更新日期:1990-07-01 00:00:00

  • Down-regulation of MANNANASE7 gene in Brassica napus L. enhances silique dehiscence-resistance.

    abstract:KEY MESSAGE:MANNANASE7 gene in Brassica napus L. encodes a hemicellulose which located at cell wall or extracellular space and dehiscence-resistance can be manipulated by altering the expression of MANNANASE7. Silique dehiscence is an important physiological process in plant reproductive development, but causes heavy y...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-020-02638-5

    authors: Li YL,Yu YK,Zhu KM,Ding LN,Wang Z,Yang YH,Cao J,Xu LZ,Li YM,Tan XL

    更新日期:2021-02-01 00:00:00

  • Arabidopsis GIGANTEA negatively regulates chloroplast biogenesis and resistance to herbicide butafenacil.

    abstract:KEY MESSAGE:Arabidopsis GI negatively regulates chloroplast biogenesis and resistance to the herbicide butafenacil by enhanced activity and transcriptional levels of antioxidant enzymes Chloroplast biogenesis is blocked by retrograde signaling triggered by diverse internal and external cues, including sugar, reactive o...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-019-02409-x

    authors: Cha JY,Lee DY,Ali I,Jeong SY,Shin B,Ji H,Kim JS,Kim MG,Kim WY

    更新日期:2019-07-01 00:00:00

  • In planta 2,3,5 truodobenzoic acid treatment promotes high frequency and routine in vitro regeneration of sugarbeet (Beta vulgaris L.) plants.

    abstract::The effect ofin planta treatments with auxin inhibitors such as 2,3,5 triiodobenzoic acid (TIBA) on regeneration of plantsin vitro is not known. Here, we show the beneficial effect of preconditioning sugarbeet plants in the greenhouse with TIBA (3 mg/1) for efficientin vitro plant regeneration via a callus phase from ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF01890855

    authors: Roussy I,Dubois F,Sangwan RS,Sangwan-Norreel BS

    更新日期:1996-12-01 00:00:00

  • Hydrogen sulfide enhances nitric oxide-induced tolerance of hypoxia in maize (Zea mays L.).

    abstract:KEY MESSAGE:Our data present H 2 S in a new role, serving as a multi-faceted transducer to different response mechanisms during NO-induced acquisition of tolerance to flooding-induced hypoxia in maize seedling roots. Nitric oxide (NO), serving as a secondary messenger, modulates physiological processes in plants. Recen...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-016-2037-4

    authors: Peng R,Bian Z,Zhou L,Cheng W,Hai N,Yang C,Yang T,Wang X,Wang C

    更新日期:2016-11-01 00:00:00

  • Embryogenic cell suspensions of Triticum aestivum x Leymus angustus F1 hybrids: characterization and plant regeneration.

    abstract::Embryogenic cell suspension cultures were established from Triticum aestivum X Leymus angustus F1 hybrids, using compact nodular calli derived from inflorescence segments. Calli originating from leaf segments did not give rise to stable cell suspensions. Growth measurements of the cell suspensions revealed that they c...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00235258

    authors: Tabaeizadeh Z,Campeau N

    更新日期:1992-03-01 00:00:00

  • Production of aposporous gametophytes and calli from Pteris vittata L. pinnae strips cultured in vitro.

    abstract::Murashige and Skoog's modified medium in 1% Difco Bacto-agar supplemented with sugar alcohols (sorbsitol, mannitol), growth regulators (1-naphthalenacetic acid, 2,4-dichlorophenoxyacetic acid, benzyladenine, kinetin) and sugars (fructose, glucose, sucrose) induced aposporous gametophytes from pinnae of Pteris vittata ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00232608

    authors: Kwa SH,Wee YC,Loh CS

    更新日期:1991-10-01 00:00:00

  • Early nodulin 93 protein gene: essential for induction of somatic embryogenesis in oil palm.

    abstract:KEY MESSAGE:Transcript profiling during the early induction phase of oil palm tissue culture and RNAi studies in a model somatic embryogenesis system showed that EgENOD93 expression is essential for somatic embryogenesis. Micropropagation of oil palm through tissue culture is vital for the generation of superior and un...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-020-02571-7

    authors: Chan PL,Rose RJ,Abdul Murad AM,Zainal Z,Ong PW,Ooi LC,Low EL,Ishak Z,Yahya S,Song Y,Singh R

    更新日期:2020-11-01 00:00:00

  • Shoot regeneration from cultured root explants of spinach (Spinacia oleracea L.): a system for Agrobacterium transformation.

    abstract::A reliable plant regeneration system is described for the production of adventitious shoots from root explants of spinach. Explants from roots of axenic shoots and roots induced on cultured hypocotyl explants were used for adventitious shoot induction. Explants from apical, middle and basal root regions were incubated...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s002990050359

    authors: Knoll KA,Short KC,Curtis IS,Power JB,Davey MR

    更新日期:1997-12-01 00:00:00

  • Promotion of flowering and morphological alterations in Atropa belladonna transformed with a CaMV 35S-rolC chimeric gene of the Ri plasmid.

    abstract::Kanamycin-resistant plants of belladonna (Atropa belladonna) were obtained after Agrobacterium mediated transformation. When a rolC gene, which is one of the loci located on Ri plasmid of Agrobacterium rhizogenes, was co-introduced with a kanamycin resistant (NPT II) gene under control of a cauliflower mosaic virus 35...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00232412

    authors: Kurioka Y,Suzuki Y,Kamada H,Harada H

    更新日期:1992-12-01 00:00:00

  • An efficient method for flow cytometric analysis of pollen and detection of 2n nuclei in Brassica napus pollen.

    abstract::A simple and reliable method was developed for isolating pollen nuclei from Brassica napus and Triticum aestivum for DNA analysis using flow cytometry. The nuclei were released from pollen by ultrasonic treatment. The isolated nuclei following filtration through nylon mesh and a purification procedure were suitable fo...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-004-0830-y

    authors: Pan G,Zhou Y,Fowke LC,Wang H

    更新日期:2004-10-01 00:00:00

  • Regeneration of plants from mesophyll protoplasts of the wild crucifer Eruca sativa Lam.

    abstract::Protoplasts isolated from mesophyll cells of Eruca sativa Lam., cultured on suitable medium, underwent sustained cell divisions to form calli. The plating efficiency was found to be 0.4%. The protoplast-derived calli subsequently produced plantlets through organogenesis (15.71%) and somatic embryogenesis (11.25%). Reg...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00272790

    authors: Sikdar SR,Chatterjee G,Das S,Sen SK

    更新日期:1987-12-01 00:00:00

  • Saponin production by cultures of Panax ginseng transformed with Agrobacterium rhizogenes.

    abstract::Hairy root culture of Ginseng (Panax ginseng) was established after roots were induced on callus following infection with Agrobacterium rhizogenes. The transformed cultures of ginseng could be subcultured as an axenic root culture in the absence of phytohormones, and grew with extensive lateral branches more rapidly t...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00272780

    authors: Yoshikawa T,Furuya T

    更新日期:1987-12-01 00:00:00

  • Effects of drought-re-watering-drought on the photosynthesis physiology and secondary metabolite production of Bupleurum chinense DC.

    abstract:KEY MESSAGE:Drastic changes in soil water content can activate the short-term high expression of key enzyme-encoding genes involved in secondary metabolite synthesis thereby increasing the content of secondary metabolites. Bupleurum chinense DC. is a traditional medicinal herb that is famous for its abundant saikosapon...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-019-02436-8

    authors: Yang L,Zhao Y,Zhang Q,Cheng L,Han M,Ren Y,Yang L

    更新日期:2019-09-01 00:00:00

  • In vitro adventitious shoot production inPaulownia.

    abstract::In vitro adventitious shoot formation was examined in petioles and laminae ofPaulownia elongata, P. fortunei, andP. 'Henan 1'. Fully expanded, dark green, thick, older leaves were healthier and exhibited greater callus and shoot production than did young leaves. The ten clones used varied greatly in adventitious shoot...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF01088288

    authors: Bergmann BA,Moon HK

    更新日期:1997-02-01 00:00:00

  • Characterization of a chalcone synthase (CHS) flower-specific promoter from Lilium orential 'Sorbonne'.

    abstract::The first enzyme in the flavonoid pathway, chalcone synthase, is encoded by a gene (CHS) whose expression is normally under developmental control. In our previous studies, an 896-bp promoter region of a flower-specific CHS gene was isolated from Lilium orential 'Sorbonne', and designated as PLoCHS. Here, the PLoCHS pr...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-011-1124-9

    authors: Liu Y,Lou Q,Xu W,Xin Y,Bassett C,Wang Y

    更新日期:2011-12-01 00:00:00