Abstract:
:Reliable regeneration protocols for Eucalyptus camaldulensis using leaf explants from in vitro-grown plants have been developed. Out of the 24 clones tested 13 were regenerated and of these, 6 showed regeneration from more than 60% of the explants. Identical protocols were also successful in the regeneration of some clones of E. microtheca, E. ochrophloia, E. grandis and E. marginata, but at lower frequencies. Co-cultivation of E. camaldulensis leaf explants with Agrobacterium tumefaciens strains carrying a kanamycin resistance gene and the reporter gene β-glucuronidase (GUS), followed by selection on kanamycin at 9 mg l-1, allowed the selection of transformed shoots that could be rooted on selective media. Transformation of the plants was verified by staining for the GUS enzyme in various plant tissues, NptII assays and by Southern blotting on isolated DNA using specific probes for both the GUS and selectable marker genes. Transformed tissue was obtained with 5 clones of E. camaldulensis tested and a number of A. tumefaciens strains. However, only 1 clone regenerated transformed whole plants reliably.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Mullins KV,Llewellyn DJ,Hartney VJ,Strauss S,Dennis ESdoi
10.1007/s002990050321subject
Has Abstractpub_date
1997-09-01 00:00:00pages
787-791issue
11eissn
0721-7714issn
1432-203Xpii
10.1007/s002990050321journal_volume
16pub_type
杂志文章abstract::A method has been devised which allows the rapid production of fine suspension cultures of small aggregate size from suspension cultures of large average aggregate size, such as those of Capsicum frutescens. The method, which uses a Waring blender for aseptic homogenisation of cultures, has also been shown to be effec...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269538
更新日期:1988-10-01 00:00:00
abstract::Self-incompatibility (SI) has been studied extensively at the molecular level in Solanaceae, Rosaceae and Scrophulariaceae, all of which exhibit gametophytic self-incompatibility (GSI). In the present study, four PpsS-haplotypes (Prunus pseudocerasus S-haplotypes) comprising at least two genes, i.e., PpsS-RNase (P. ps...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-008-0528-7
更新日期:2008-06-01 00:00:00
abstract:UNLABELLED:Calmodulin-binding proteins (CBPs) have been known to be involved in both biotic and abiotic stress responses. Recently, two closely related CBPs, Arabidopsis SAR Deficient 1 and CBP60g, were found to belong to a new family of transcription factors that regulate salicylic acid (SA) biosynthesis triggered by ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-012-1247-7
更新日期:2012-07-01 00:00:00
abstract::The production of cybrids, combining nucleus of a species with alien cytoplasmic organelles, is a valuable method used for improvement of various crops. Several citrus cybrids have been created by somatic hybridization. These genotypes are interesting models to analyze the impact of cytoplasmic genome change on nuclea...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1014-1
更新日期:2011-06-01 00:00:00
abstract::Cotyledons from aseptically grown seedlings of sainfoin (Onobrychis viciifolia Scop.) were used as explant material to grow callus tissue for periods of 21 and 31 days. The formation of cells containing condensed tannins was induced by adding a range of BAP concentrations to an mB5 culture medium containing 2,4-D. Aft...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269314
更新日期:1988-05-01 00:00:00
abstract::Capsella bursa-pastoris (L.) Medic (2n = 4x = 32) is a natural double-low (erucic acid < 1%, glucosinolates < 30 micromol/g) germplasm and shows high degree of resistance to Sclerotinia sclerotiorum. Hybridizations were carried out between two Brassica species viz. B. rapa (2n = 20) and B. napus (2n = 38) as female an...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-007-0392-x
更新日期:2007-10-01 00:00:00
abstract:KEY MESSAGE:We demonstrate the role of DREB1A transcription factor in better root and shoot partitioning and higher transpiration efficiency in transgenic chickpea under drought stress Chickpea (Cicer arietinum L.) is mostly exposed to terminal drought stress which adversely influences its yield. Development of cultiva...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-014-1699-z
更新日期:2015-02-01 00:00:00
abstract::Transgenic papaya (Carica papaya L.) plants were regenerated from embryogenic cultures that were cocultivated with a disarmed C58 strain of Agrobacterium tumefaciens containing one of the following binary cosmid vectors: pGA482GG or pGA482GG/cpPRV-4. The T-DNA region of both binary vectors includes the chimeric genes ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00237128
更新日期:1993-03-01 00:00:00
abstract::Taro bacilliform virus (TaBV) is a pararetrovirus of the genus Badnavirus which infects the monocotyledonous plant, taro ( Colocasia esculenta). A region of the TaBV genome spanning nucleotides 6,281 to 12 (T1200), including the 3' end of open reading frame 3 (ORF 3) and the intergenic region to the end of the tRNA(me...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0621-x
更新日期:2003-08-01 00:00:00
abstract:UNLABELLED:The quality and productivity of chrysanthemum are severely compromised by various abiotic stresses. Here, we describe the isolation of CdICE1 from Chrysanthemum dichrum using RACE PCR, which shared identical nucleotide of ICE1 ORF from Chrysanthemum grandiflorum variety 'Jinba'. CdICE1 contains a conserved b...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-012-1288-y
更新日期:2012-09-01 00:00:00
abstract::Shoot cultures ofAtractylodes lancea DC. (Compositae) have been established by inoculating the flower bud on Linsmaier-Skoog's medium supplemented with 10(-5) M naphthaleneacetic acid and 10(-5) M 6-benzyl-aminopurine. Shoots were multiplied on a medium containing 10(-6) M 6-benzylaminopurine. Propagated shoots rooted...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF02441005
更新日期:1984-06-01 00:00:00
abstract::RNA interference (RNAi) is a sequence-specific, posttranscriptional gene silencing (PTGS) process in plants that is mediated by dsRNA homologous to the silenced gene(s). In this study, we report an efficient method to produce dsRNA using a bacterial expression system. Two fragments of the Sugarcane Mosaic Virus (SCMV)...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-010-0911-z
更新日期:2010-11-01 00:00:00
abstract:KEY MESSAGE:The recovery of non-functional-enhanced green fluorescence protein can be used as indicator to facilitate the identification of mutants generated by CRISPR/Cas9. The CRISPR/Cas9 system is a powerful tool for genome editing and it has been employed to knock out genes of interest in multiple plant species. Id...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-019-02465-3
更新日期:2019-12-01 00:00:00
abstract::Cotyledon explants from zygotic embryos of Panax ginseng produced somatic embryos on Murashige and Skoog basal medium without growth regulators. Somatic embryos developed directly from epidermal cells at the cotyledon base. Somatic embryos were always formed from the side of the cotyledon opposite to the one attached ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050331
更新日期:1997-10-01 00:00:00
abstract::Autoclaving is a standard procedure for sterilizing nutrient media for plant tissue cultures. Most tissue cultures are grown at pH 5.2 to 5.8 with pH adjustments being made prior to autoclaving. This paper reports that there are significant differences between initial pH levels and pH levels following autoclaving, par...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269825
更新日期:1986-08-01 00:00:00
abstract::Self-incompatibility (SI) discriminating self- and non-self pollen is regulated by S-locus genes in Brassica. In most of the S haplotypes, a highly polymorphic S-locus glycoprotein ( SLG) gene is tightly linked to genes for the SI determinants, S-receptor kinase ( SRK) and SP11, although the precise function of SLG in...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0598-5
更新日期:2003-06-01 00:00:00
abstract::The effect of the oxygen concentration in the aeration gas on regeneration from rice cells in bioreactor cultures was investigated. The efficiency of regeneration in cultures aerated with over 40% oxygen was higher than that in a flask culture. In the case of a culture in which the dissolved oxygen(DO) was saturated b...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232217
更新日期:1996-06-01 00:00:00
abstract::Leaf blade tissue of maize inbred lines B73 and Mo17 was analyzed for intraspecific genetic variability in the heat shock response. The maize inbreds were characterized for acquired thermal tolerance and patterns of heat shock protein synthesis. The leakage conductivity assay of membrane stability during stress indica...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233096
更新日期:1992-10-01 00:00:00
abstract::The sequences of the 3' untranslated region (UTR) of the manganese superoxide dismutase (MnSOD) genes in wheat (Triticum aestivum) were found to be quite variable with different predicted thermostabilities. The degradation rates of the 3' UTR variants and the coding region were measured following exposure to endogenou...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-005-0046-9
更新日期:2006-03-01 00:00:00
abstract:: An effective method has been developed for the stable transformation and regeneration of Cavendish banana (Musa spp. AAA group) cv 'Grand Nain' by microprojectile bombardment. Embryogenic cell suspensions were initiated using immature male flowers as the explant. Cells were co-bombarded with the neomycin phosphotrans...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050004
更新日期:2000-01-01 00:00:00
abstract::Shoot cultures of Allium sativum L. were established from stem-tip and scape-tip. Various combinations of media, growth regulators were compared. The best establishment and proliferation of stem-tip and scape-tip explants were obtained on MS medium containing 0.5 mg l(-1) NAA and 0.5 mg l(-1) KT. The roots were produc...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233301
更新日期:1994-11-01 00:00:00
abstract:KEY MESSAGE:Tetraploid clementine displays mainly tetrasomic inheritance. Genetic structures of 2n SDR and 2 × gametes from DD clementine are complementary and will guides triploids citrus breeding strategies. Triploid breeding is developed worldwide to create new seedless cultivars. Citrus triploid hybrids can be reco...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-016-1972-4
更新日期:2016-08-01 00:00:00
abstract::Growth inhibition towards Rhizopus nigricans, Fusarium oxysporum f. sp. radicis-lycopersici, Verticillium albo-atrum and Pythium ultimum was observed in vitro using a purified chitosanase from an actinomycete, Streptomyces sp, strain N174. The corresponding gene, with its own signal peptide, was inserted into pBI121.7...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00193724
更新日期:1995-12-01 00:00:00
abstract:: The potential of ultrasonic standing waves for trapping suspended particles was utilized successfully for differential size fractionation of plant somatic embryos. In a flow-through resonator equipped with a 200-kHz piezoceramic transducer, embryos of different sizes corresponding to different developmental stages co...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990000234
更新日期:2000-11-01 00:00:00
abstract::The efficacy of benzyladenine (BA) to induce multiple shoots from seed explants of chickpea (Cicer arietinum L.) was assessed. Shoot differentiation was influenced by the type of seed explant, genotype and concentration of BA. Orientation of the explant also strongly influenced the shoot regeneration response. The opt...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF01142325
更新日期:1997-05-01 00:00:00
abstract::Site-specific recombination has been developed into a genetic engineering tool for higher eukaryotes. The manipulation of newly introduced DNA is now possible in the course of genetic transformation procedures, thus making the process more predictable and reliable. Also, a wide variety of chromosomal rearrangements us...
journal_title:Plant cell reports
pub_type: 杂志文章,评审
doi:10.1007/s00299-003-0616-7
更新日期:2003-06-01 00:00:00
abstract::Two clones of Populus nigra L. were tested in vivo and in vitro for their susceptibility to three different Agrobacterium tumefaciens wild-type strains evaluating number and size of resulting calluses. Strain C58 proved to be the most virulent.Various parameters affecting Agrobacterium-mediated transformation of P. ni...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233315
更新日期:1994-02-01 00:00:00
abstract::Conditions necessary for the isolation and culture of protoplasts from suspension cultures of sugar, fodder and garden beets were investigated. Good yields of protoplasts were obtained by treating cells with a mixture of cellulase, Macerozyme and Driselase enzymes. Nutritional requirements of beet protoplasts were fou...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269896
更新日期:1985-12-01 00:00:00
abstract::Somatic hybrids were obtained by the symmetric fusion of embryogenic callus cells from tetraploid 'Mame' kumquat [Fortunella hindsii (Champ.) Swing.] and mesophyll cells from diploid trifoliate orange [Poncirus trifoliata (L.) Raf.]. Southern blot analysis of three regenerants revealed that they carried specific rDNA ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF01146782
更新日期:1997-03-01 00:00:00
abstract::We report here an efficient Arabidopsis leafdisc transformation protocol yielding an average transformation frequency of 1.6 transgenic shoots per leaf explant 4 weeks after the bacterial infection period. Subsequent cultivation in vitro is such that a high percentage (85-90%) of the primary transformants produces see...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232455
更新日期:1996-04-01 00:00:00