DREB1A overexpression in transgenic chickpea alters key traits influencing plant water budget across water regimes.

Abstract:

KEY MESSAGE:We demonstrate the role of DREB1A transcription factor in better root and shoot partitioning and higher transpiration efficiency in transgenic chickpea under drought stress Chickpea (Cicer arietinum L.) is mostly exposed to terminal drought stress which adversely influences its yield. Development of cultivars for suitable drought environments can offer sustainable solutions. We genetically engineered a desi-type chickpea variety to ectopically overexpress AtDREB1A, a transcription factor known to be involved in abiotic stress response, driven by the stress-inducible Atrd29A promoter. From several transgenic events of chickpea developed by Agrobacterium-mediated genetic transformation, four single copy events (RD2, RD7, RD9 and RD10) were characterized for DREB1A gene overexpression and evaluated under water stress in a biosafety greenhouse at T6 generation. Under progressive water stress, all transgenic events showed increased DREB1A gene expression before 50 % of soil moisture was lost (50 % FTSW or fraction of transpirable soil water), with a faster DREB1A transcript accumulation in RD2 at 85 % FTSW. Compared to the untransformed control, RD2 reduced its transpiration in drier soil and higher vapor pressure deficit (VPD) range (2.0-3.4 kPa). The assessment of terminal water stress response using lysimetric system that closely mimics the soil conditions in the field, showed that transgenic events RD7 and RD10 had increased biomass partitioning into shoot, denser rooting in deeper layers of soil profile and higher transpiration efficiency than the untransformed control. Also, RD9 with deeper roots and RD10 with higher root diameter showed that the transgenic events had altered rooting pattern compared to the untransformed control. These results indicate the implicit influence of rd29A::DREB1A on mechanisms underlying water uptake, stomatal response, transpiration efficiency and rooting architecture in water-stressed plants.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Anbazhagan K,Bhatnagar-Mathur P,Vadez V,Dumbala SR,Kishor PB,Sharma KK

doi

10.1007/s00299-014-1699-z

subject

Has Abstract

pub_date

2015-02-01 00:00:00

pages

199-210

issue

2

eissn

0721-7714

issn

1432-203X

journal_volume

34

pub_type

杂志文章
  • Genetic analysis of T-DNA insertions into the tobacco genome.

    abstract::A genetic test was performed on seeds from 283 transgenic tobacco plants obtained by T-DNA transformation. Seeds from self-fertilized transgenic plants were germinated on kanamycin-containing medium, and the percentage of seeds which germinated, as well as the ratio of kanamycin-resistant to kanamycin-sensitive seedli...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00272760

    authors: Heberle-Bors E,Charvat B,Thompson D,Schernthaner JP,Barta A,Matzke AJ,Matzke MA

    更新日期:1988-12-01 00:00:00

  • Gynogenic plant regeneration from unpollinated flower explants of Eragrostis tef (Zuccagni) Trotter.

    abstract::Tef [Eragrostis tef (Zucc.) Trotter] is the most important cereal in Ethiopia. In its wild relative E. mexicana, regeneration of six green plants resulted from culture of 121 non-pollinated immature pistils. In the allotetraploid crop species tef, however, only callus and root formation was obtained by this method. By...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-006-0200-z

    authors: Gugsa L,Sarial AK,Lörz H,Kumlehn J

    更新日期:2006-12-01 00:00:00

  • Plant regeneration from callus and protoplasts of Brassica nigra (IC 257) through somatic embryogenesis.

    abstract::A method to obtain plants from embryogenic callus of Brassica nigra and protoplasts of hypocotyl expiants is described. Callus was initiated on Murashige and Skoog medium containing kinetin (kn) and 2,4-dichlorophenoxy acetic acid (2,4-D). Lowering of auxin induced embryo formation. Supplementation with gibberellic ac...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00232265

    authors: Gupta V,Agnihotri A,Jagannathan V

    更新日期:1990-12-01 00:00:00

  • Stability of tissue culture medium pH as a function of autoclaving, time, and cultured plant material.

    abstract::Autoclaving is a standard procedure for sterilizing nutrient media for plant tissue cultures. Most tissue cultures are grown at pH 5.2 to 5.8 with pH adjustments being made prior to autoclaving. This paper reports that there are significant differences between initial pH levels and pH levels following autoclaving, par...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00269825

    authors: Skirvin RM,Chu MC,Mann ML,Young H,Sullivan J,Fermanian T

    更新日期:1986-08-01 00:00:00

  • Separation of root nodule cells and identification of tissue-specific genes.

    abstract::The interior tissues of the alfalfa (Medicago sativa) root nodule differ in form and function from the peripheral layers. The interior tissues are specialized for the fixation of nitrogen in cells infected by rhizobia. In contrast, the peripheral nodule tissues perform roles that assist the interior tissues: they prov...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s002990000216

    authors: Ganter G,Duquette M,Dunn K

    更新日期:2000-09-01 00:00:00

  • SA and ROS are involved in methyl salicylate-induced programmed cell death in Arabidopsis thaliana.

    abstract::Programmed cell death (PCD) is a genetically encoded, active process that results in the death of individual cells, tissues, or whole organs, which plays an important role in the life cycles of plants and animals. Previous studies show that methyl salicylate (MeSA) is a defense signal molecular associated with systemi...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-011-1031-0

    authors: Yun LJ,Chen WL

    更新日期:2011-07-01 00:00:00

  • Post-Golgi protein traffic in the plant secretory pathway.

    abstract::The Golgi apparatus in plants is organized as a multitude of individual stacks that are motile in the cytoplasm and in close association with the endoplasmic reticulum (ER) (Boevink et al. in Plant J 15:441-447, 1998). These stacks operate as a sorting centre for cargo molecules, providing modification and redirection...

    journal_title:Plant cell reports

    pub_type: 杂志文章,评审

    doi:10.1007/s00299-007-0390-z

    authors: Hanton SL,Matheson LA,Chatre L,Rossi M,Brandizzi F

    更新日期:2007-09-01 00:00:00

  • A promoter derived from taro bacilliform badnavirus drives strong expression in transgenic banana and tobacco plants.

    abstract::Taro bacilliform virus (TaBV) is a pararetrovirus of the genus Badnavirus which infects the monocotyledonous plant, taro ( Colocasia esculenta). A region of the TaBV genome spanning nucleotides 6,281 to 12 (T1200), including the 3' end of open reading frame 3 (ORF 3) and the intergenic region to the end of the tRNA(me...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-003-0621-x

    authors: Yang IC,Iommarini JP,Becker DK,Hafner GJ,Dale JL,Harding RM

    更新日期:2003-08-01 00:00:00

  • Inefficient expression of the DNA-binding domain of GAL4 in transgenic plants.

    abstract::The DNA-binding domain of the yeast transcriptional activator GAL4 was expressed in transgenic tobacco plants in order to attempt to obtain specific repression of reporter genes. Expression of the GUS and the NPTII gene was essentially the same regardless of the presence of a gene encoding a functional GAL4 DNA-bindin...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00232920

    authors: Reichel C,Feltkamp D,Walden R,Steinbiß HH,Schell J,Rosahl S

    更新日期:1995-10-01 00:00:00

  • In vivo nitrate reductase activity in dark- and light-grown sugarcane callus.

    abstract::The in vivo nitrate reductase activity in 8 day old dark-grown sugarcane callus was over three fold that of the light-grown callus. NADH (0.3 mM) in the reaction system, increased the in vivo nitrate reductase activity by more than two fold both in the dark- and the light-grown callus tissues. The NADH dependence of n...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00270207

    authors: Khan BM,Dwivedi UN,Rawal SK,Mascarenhas AF

    更新日期:1984-08-01 00:00:00

  • Gene silencing studies in the gymnosperm species Pinus radiata.

    abstract::A biolistic transformation procedure was used to transform embryogenic Pinus radiata tissue with constructs containing the Zea mays UBI1 (ubiquitin)-promoter followed by the P. radiata CAD (cinnamyl alcohol dehydrogenase) cDNA in sense or anti-sense orientation or in the form of an inverted-repeat. The effect of the d...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-004-0911-y

    authors: Wagner A,Phillips L,Narayan RD,Moody JM,Geddes B

    更新日期:2005-05-01 00:00:00

  • Biotechnology of oil palm: strategies towards manipulation of lipid content and composition.

    abstract::Oil palm is a major economic crop for Malaysia. The major challenges faced by the industry are labor shortage, availability of arable land and unstable commodity price. This has caused the industry to diversify its applications into higher value products besides increasing its yield. While conventional breeding has it...

    journal_title:Plant cell reports

    pub_type: 杂志文章,评审

    doi:10.1007/s00299-014-1722-4

    authors: Parveez GK,Rasid OA,Masani MY,Sambanthamurthi R

    更新日期:2015-04-01 00:00:00

  • An ethylene response factor (ERF5) promoting adaptation to drought and salt tolerance in tomato.

    abstract::A novel member of the AP2/ERF transcription factor family, SlERF5, was identified from a tomato mature leaf cDNA library screen. The complete DNA sequence of SlERF5 encodes a putative 244-amino acid DNA-binding protein which most likely acts as a transcriptional regulator and is a member of the ethylene responsive fac...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-011-1170-3

    authors: Pan Y,Seymour GB,Lu C,Hu Z,Chen X,Chen G

    更新日期:2012-02-01 00:00:00

  • Opportunities and constraints to biotechnological applications in the Caribbean: transgenic papayas in Jamaica and Venezuela.

    abstract::In this opinion article, we briefly review the status of crop biotechnology research-with emphasis on the development of GM crops-in Jamaica and Venezuela. We focus on the transgenic papayas developed for both countries, and examine the factors hindering not only the development and application of this biotechnologica...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-010-0988-4

    authors: Fermin G,Tennant P

    更新日期:2011-05-01 00:00:00

  • Recovery of the non-functional EGFP-assisted identification of mutants generated by CRISPR/Cas9.

    abstract:KEY MESSAGE:The recovery of non-functional-enhanced green fluorescence protein can be used as indicator to facilitate the identification of mutants generated by CRISPR/Cas9. The CRISPR/Cas9 system is a powerful tool for genome editing and it has been employed to knock out genes of interest in multiple plant species. Id...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-019-02465-3

    authors: Ren C,Guo Y,Gathunga EK,Duan W,Li S,Liang Z

    更新日期:2019-12-01 00:00:00

  • Isolation and characterization of a chlorophyll degradation regulatory gene from tall fescue.

    abstract::The non-yellowing gene (NYE1), initially identified from Arabidopsis, is a key regulatory gene responsible for chlorophyll degradation during senescence. Here, FaNYE1, an orthologue of AtNYE1, was further identified from a major type of cool-season turf grass, tall fescue (Festuca arundinacea Schreb.), by RACE-PCR. It...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-011-1028-8

    authors: Wei Q,Guo Y,Kuai B

    更新日期:2011-07-01 00:00:00

  • CRISPR/Cas9 mutations in the rice Waxy/GBSSI gene induce allele-specific and zygosity-dependent feedback effects on endosperm starch biosynthesis.

    abstract:KEY MESSAGE:Induced mutations in the waxy locus in rice endosperm did not abolish GBSS activity completely. Compensatory mechanisms in endosperm and leaves caused a major reprogramming of the starch biosynthetic machinery. The mutation of genes in the starch biosynthesis pathway has a profound effect on starch quality ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-019-02388-z

    authors: Pérez L,Soto E,Farré G,Juanos J,Villorbina G,Bassie L,Medina V,Serrato AJ,Sahrawy M,Rojas JA,Romagosa I,Muñoz P,Zhu C,Christou P

    更新日期:2019-03-01 00:00:00

  • Functional characterization of a stilbene synthase gene using a transient expression system in planta.

    abstract::The expression and functionality of a resveratrol synthase (RS) gene from peanut (Arachis hypogaea) was studied using an Agrobacterium tumefaciens-mediated transient expression system in Nicotiana benthamiana leaves. Functional analysis of RS was demonstrated by tracking its expression during 96 h. To measure the tran...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-008-0664-0

    authors: Condori J,Medrano G,Sivakumar G,Nair V,Cramer C,Medina-Bolivar F

    更新日期:2009-04-01 00:00:00

  • Virus-induced gene silencing (VIGS)-mediated functional characterization of two genes involved in lignocellulosic secondary cell wall formation.

    abstract:KEY MESSAGE:Functional characterization of two tobacco genes, one involved in xylan synthesis and the other, a positive regulator of secondary cell wall formation, is reported. Lignocellulosic secondary cell walls (SCW) provide essential plant materials for the production of second-generation bioethanol. Therefore, tho...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-016-2039-2

    authors: Pandey SK,Nookaraju A,Fujino T,Pattathil S,Joshi CP

    更新日期:2016-11-01 00:00:00

  • Optimization of gene transfer into barley (Hordeum vulgare L.) mature embryos by tissue electroporation.

    abstract:: This study was conducted to detect the optimum conditions for DNA transfer into mature embryos of barley via electroporation. Cultured mature embryos of barley were directly electroporated in the presence of the pBI 121 vector carrying both the β-glucuronidase (GUS) and neomycin phosphotransferase II (npt II) genes. ...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s002999900182

    authors: Gürel F,Gözükirmizi N

    更新日期:2000-07-01 00:00:00

  • Plant regeneration through somatic embryogenesis from suspension cultures of a minor millet, Paspalum scrobiculatum.

    abstract::Compact, friable and embryogenic calli were initiated from immature inflorescences and young leaf bases of one week old seedlings of Paspalum scrobiculatum cultured on MS medium supplemented with 2,4-D. A stable, embryogenic suspension culture was initiated from these calli and maintained in a liquid version of the sa...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00274134

    authors: Nayak P,Sen SK

    更新日期:1989-05-01 00:00:00

  • Galactoglucomannan oligosaccharides are assumed to affect tracheary element formation via interaction with auxin in Zinnia xylogenic cell culture.

    abstract:KEY MESSAGE:Galactoglucomannan oligosaccharides seem to interact with auxin in xylogenic cell culture, thus influencing mainly metaxylem-like tracheary element differentiation depending on timing with hormones and the process kinetics. Complex mapping of Zinnia mesophyll cell transdifferentiation into tracheary element...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-012-1379-9

    authors: Kákošová A,Digonnet C,Goffner D,Lišková D

    更新日期:2013-04-01 00:00:00

  • Plant regeneration from immature embryos of peanut.

    abstract::Plant regeneration from immature embryos of peanut (Arachis hypogaea L.) can be accomplished through somatic embryogenesis. The highest frequency of somatic embryo formation occurred on B5 medium plus 0.5-1.0 mg/l picloram. Shoots and plants developed from the somatic embryos only after extended culture on basal mediu...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00778536

    authors: Ozias-Akins P

    更新日期:1989-04-01 00:00:00

  • Tissue culture-induced flower-color changes in Saintpaulia caused by excision of the transposon inserted in the flavonoid 3', 5' hydroxylase (F3'5'H) promoter.

    abstract::The variegated Saintpaulia cultivar Thamires (Saintpaulia sp.), which has pink petals with blue splotches, is generally maintained by leaf cuttings. In contrast, tissue culture-derived progeny of the cultivar showed not only a high percentage of mutants with solid-blue petals but also other solid-color variants, which...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-011-1016-z

    authors: Sato M,Kawabe T,Hosokawa M,Tatsuzawa F,Doi M

    更新日期:2011-05-01 00:00:00

  • A method for the rapid production of fine plant cell suspension cultures.

    abstract::A method has been devised which allows the rapid production of fine suspension cultures of small aggregate size from suspension cultures of large average aggregate size, such as those of Capsicum frutescens. The method, which uses a Waring blender for aseptic homogenisation of cultures, has also been shown to be effec...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00269538

    authors: Williams PD,Wilkinson AK,Lewis JA,Black GM,Mavituna F

    更新日期:1988-10-01 00:00:00

  • The effects of acetosyringone and pH on Agrobacterium-mediated transformation vary according to plant species.

    abstract::Expiants of five plant species (Allium cepa, Antirrhinum majus, Brassica campestris. Glycine max, and Nicotiana tabacum) were co-cultivated with three Agrobacterium tumefaciens strains under different conditions to assess the effects of acetosyringone and medium pH on strain virulence. Tumours were incited on all dico...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00235354

    authors: Godwin I,Todd G,Ford-Lloyd B,Newbury HJ

    更新日期:1991-04-01 00:00:00

  • Two EST-derived marker systems for cultivar identification in tree peony.

    abstract::Tree peony (Paeonia suffruticosa Andrews), a woody deciduous shrub, belongs to the section Moutan DC. in the genus of Paeonia of the Paeoniaceae family. To increase the efficiency of breeding, two EST-derived marker systems were developed based on a tree peony expressed sequence tag (EST) database. Using target region...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-011-1164-1

    authors: Zhang JJ,Shu QY,Liu ZA,Ren HX,Wang LS,De Keyser E

    更新日期:2012-02-01 00:00:00

  • A simple system for pea transformation.

    abstract::The lateral cotyledonary meristems of germinatingPisum sativum cv. Puget seeds were used to develop a reproducibleAgrobacterium tumefaciens-mediated transformation system. This procedure exhibits distinct advantages over those previously reported, in that it uses dry seed as starting material, and the highly regenerab...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF01142315

    authors: Bean SJ,Gooding PS,Mullincaux PM,Davies DR

    更新日期:1997-05-01 00:00:00

  • Functional characterization of a chalcone synthase from the liverwort Plagiochasma appendiculatum.

    abstract:KEY MESSAGE:A chalcone synthase gene ( PaCHS ) was isolated and functionally characterized from liverwort. The ectopic expression of PaCHS in Marchantia paleacea callus raised the flavonoids content. Chalcone synthase (CHS; EC 2.3.1.74) is pivotal for the biosynthesis of flavonoid and anthocyanin pigments in plants. It...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/s00299-014-1702-8

    authors: Yu HN,Wang L,Sun B,Gao S,Cheng AX,Lou HX

    更新日期:2015-02-01 00:00:00

  • Efficient plant regeneration from hypocotyl protoplasts of broccoli (Brassica oleracea L. ssp. italica Plenck).

    abstract::We have assessed the capacity of cultured protoplasts from two tissue sources of several commercially-grown broccoli cultivars to regenerate plants. A procedure that employs hypocotyl protoplasts and a culture medium with a high NAA:2,4-D auxin ratio was developed. The procedure permits highly efficient formation of c...

    journal_title:Plant cell reports

    pub_type: 杂志文章

    doi:10.1007/BF00232858

    authors: Kao HM,Keller WA,Gleddie S,Brown GG

    更新日期:1990-10-01 00:00:00