Gynogenic plant regeneration from unpollinated flower explants of Eragrostis tef (Zuccagni) Trotter.

abstract：KEY MESSAGE:MicroRNAs have higher expression stability than protein-coding genes in B. napus seeds and are therefore good reference genes for miRNA and mRNA RT-qPCR analysis. Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) has become the "gold standard" to gain insight into function of ...

journal_title：Plant cell reports

authors： Machado RD,Christoff AP,Loss-Morais G,Margis-Pinheiro M,Margis R,Körbes AP

更新日期：2015-07-01 00:00:00

abstract：:MicroRNAs (miRNAs) are small non-coding RNAs that play vital roles in plant abiotic stress responses via cleavage or translational inhibition of their target mRNAs. Populus euphratica is a typical stress-resistant sessile organism that grows in desert areas. Here, we identified sequences of 12 miRNA precursors from 11...

journal_title：Plant cell reports

authors： Qin Y,Duan Z,Xia X,Yin W

更新日期：2011-10-01 00:00:00

abstract：:Plant regeneration via somatic embryogenesis was achieved in callus cultures derived from nodal explants of in vitro grown seedlings and excised mature zygotic embryos of three bamboo species on Murashige and Skoog's (MS) basal medium supplemented with 0.5 mg/l kinetin (Kn), 2.0 mg/l 2,4-dichlorophenoxyacetic acid (2,...

journal_title：Plant cell reports

authors： Rout GR,Das P

更新日期：1994-09-01 00:00:00

abstract：:Randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers were used to analyse the genetic diversity of Portuguese Prunus dulcis cultivars and their relationship to important foreign cultivars. Of the primers tested, 6 (out of 60) RAPD and 5 (out of 18) ISSR primers were selected for th...

journal_title：Plant cell reports

authors： Martins M,Tenreiro R,Oliveira MM

更新日期：2003-08-01 00:00:00

abstract：:Rosmarinic acid (RA) is a natural antioxidant produced by cell suspension cultures of sage (Salvia officinalis L.). The growth and production of RA by these cells can be modified by the type of culture medium. Production can be increased 10-fold to attain 6.4 g.1(-1) under optimal conditions. Investigation of kinetics...

journal_title：Plant cell reports

authors： Hippolyte I,Marin B,Baccou JC,Jonard R

更新日期：1992-04-01 00:00:00

abstract：:Citrus psorosis is a serious viral disease affecting citrus trees in many countries. Its causal agent is Citrus psorosis virus (CPsV), the type member of genus Ophiovirus. CPsV infects most important citrus varieties, including oranges, mandarins and grapefruits, as well as hybrids and citrus relatives used as rootsto...

journal_title：Plant cell reports

authors： Zanek MC,Reyes CA,Cervera M,Peña EJ,Velázquez K,Costa N,Plata MI,Grau O,Peña L,García ML

更新日期：2008-01-01 00:00:00

abstract：:Somatic hybrids were obtained by the symmetric fusion of embryogenic callus cells from tetraploid 'Mame' kumquat [Fortunella hindsii (Champ.) Swing.] and mesophyll cells from diploid trifoliate orange [Poncirus trifoliata (L.) Raf.]. Southern blot analysis of three regenerants revealed that they carried specific rDNA ...

journal_title：Plant cell reports

authors： Miranda M,Motomura T,Ikeda F,Ohgawara T,Saito W,Endo T,Omura M,Moriguchi T

更新日期：1997-03-01 00:00:00

abstract：UNLABELLED:Musa acuminata ssp. burmannica, one of the wild progenitors contributing 'A genome' to the present-day dessert bananas, has a long evolutionary history intervened by human activities. In this study, ISSR markers were used to analyze the pattern of genetic variation and differentiation in 32 individuals along...

journal_title：Plant cell reports

authors： Padmesh P,Mukunthakumar S,Vineesh PS,Skaria R,Hari Kumar K,Krishnan PN

更新日期：2012-09-01 00:00:00

abstract：:In vitro plant regeneration via embryogenesis was obtained in suspension cultures of Datura innoxia Mill. Embryogenesis was induced in suspension cultures raised from callus of androgenetic origin, using LS liquid medium supplemented with 0.22 mg/l 2,4-D. The total number of embryos formed was variable over time in cu...

journal_title：Plant cell reports

authors： Sharma VK,Jethwani V,Kothari SL

更新日期：1993-08-01 00:00:00

abstract：:This report describes the successful plant regeneration via somatic embryogenesis from immature zygotic embryos of Cryptomeria japonica D. Don. For the induction of embryogenic tissue, we determined that the optimal medium contained N6-benzyladenine and 2,4-dichlorophenoxyacetic acid. Immature zygotic embryos that wer...

journal_title：Plant cell reports

authors： Igasaki T,Sato T,Akashi N,Mohri T,Maruyama E,Kinoshita I,Walter C,Shinohara K

更新日期：2003-11-01 00:00:00

abstract：:Transgenic plants offer advantages for biomolecule production because plants can be grown on a large scale and the recombinant macromolecules can be easily harvested and extracted. We introduced an Aspergillus phytase gene into canola (Brassica napus) (line 9412 with low erucic acid and low glucosinolates) by Agrobact...

journal_title：Plant cell reports

authors： Peng RH,Yao QH,Xiong AS,Cheng ZM,Li Y

更新日期：2006-03-01 00:00:00

abstract：:Haploid plants were regenerated from cultured unfertilized ovaries of Hordeum vulgare L. (barley). Optimal response was obtained by the addition of 0.6 μM 4-chloro-2-methylphenoxyacetic acid (MCPA), 2.8 μM indole-3-acetic acid (IAA) and 4.4 μM 6-benzyladenine (BA) in the N6 medium. Further increase in the rate of call...

journal_title：Plant cell reports

authors： Castillo AM,Cistué L

更新日期：1993-01-01 00:00:00

abstract：:The aim of this study was the improvement of embryo production in wheat anther culture. Three butanol alcohols, n-butanol, sec-butanol and tert-butanol, were evaluated for their effect on microspore embryogenesis in two spring cultivars of wheat, Pavon and Caramba. Application of n-butanol, at 0.1 and 0.2% (v/v) in th...

journal_title：Plant cell reports

authors： Soriano M,Cistué L,Castillo AM

更新日期：2008-05-01 00:00:00

abstract：:The microprojectile bombardment of immature embryos has proven to be effective in transforming many indica rice varieties. One of the drawbacks of using immature embryos is the requirement of a large number of high quality immature embryos, which itself is a tedious and laborious process. To circumvent these problems,...

journal_title：Plant cell reports

authors： Sivamani E,Shen P,Opalka N,Beachy RN,Fauquet CM

更新日期：1996-01-01 00:00:00

abstract：KEY MESSAGE:We describe a steroid-inducible BABY BOOM system that improves plant regeneration in Arabidopsis leaf cultures and yields fertile plants. Regeneration of Arabidopsis thaliana plants for extended periods of time in tissue culture may result in sterile plants. We report here a novel approach for A. thaliana r...

journal_title：Plant cell reports

authors： Lutz KA,Martin C,Khairzada S,Maliga P

更新日期：2015-10-01 00:00:00

abstract：KEY MESSAGE:A plant-derived 0.3 kb constitutive promoter was obtained from AtTCTP expression analysis, and successfully applied to the expression of a selectable marker gene for production of transgenic creeping bentgrass plants. The isolation and use of an efficient promoter is essential to develop a vector system for...

journal_title：Plant cell reports

authors： Han YJ,Kim YM,Hwang OJ,Kim JI

更新日期：2015-02-01 00:00:00

abstract：:An efficient method was developed using floating membrane rafts (Liferaft(™)) for the micropropagation of Aconitum napellus (Ranunculaceae), a cut flower crop with a low natural propagation rate. This was achieved by introducing shoot tips into culture on Murashige and Skoog's (1962) solid medium, or liquid medium-sup...

journal_title：Plant cell reports

authors： Watad AA,Kochba M,Nissim A,Gaba V

更新日期：1995-03-01 00:00:00

abstract：:Site-specific recombination has been developed into a genetic engineering tool for higher eukaryotes. The manipulation of newly introduced DNA is now possible in the course of genetic transformation procedures, thus making the process more predictable and reliable. Also, a wide variety of chromosomal rearrangements us...

journal_title：Plant cell reports

authors： Lyznik LA,Gordon-Kamm WJ,Tao Y

更新日期：2003-06-01 00:00:00

abstract：:Spermidine and ornithine given to normal auxin-requiring cell suspensions of sugarbeet inhibited peroxidase secretion in the absence of Ca(2+). Habituated (organogenic or not) cells did not respond. Both compounds counteracted the Ca(2+) - promoted enzyme secretion by three cell lines. Auxins (2,4-D and BSAA) did not ...

journal_title：Plant cell reports

authors： Kevers C,Gaspar T,Penel C,Greppin H

更新日期：1985-05-01 00:00:00

abstract：:The tobacco cell line TX1 (Nicotiana tabacum) accumulates up to 10 mg cinnamoyl putrescines/g dry weight. This level was further increased under various growth limiting conditions. The negative effect of accumulated phosphate on the optimal expression of cinnamoyl putrescine biosynthesis was prevented by the fedbatch ...

journal_title：Plant cell reports

authors： Schiel O,Jarchow-Redecker K,Piehl GW,Lehmann J,Berlin J

更新日期：1984-02-01 00:00:00

abstract：:The interior tissues of the alfalfa (Medicago sativa) root nodule differ in form and function from the peripheral layers. The interior tissues are specialized for the fixation of nitrogen in cells infected by rhizobia. In contrast, the peripheral nodule tissues perform roles that assist the interior tissues: they prov...

journal_title：Plant cell reports

authors： Ganter G,Duquette M,Dunn K

更新日期：2000-09-01 00:00:00

abstract：:Brassica juncea L. is a Zn/Cd accumulator. To determine the physiological basis of its metal accumulation phenotype, the functional properties and role of the metal efflux transporter BjCET2 were investigated using transgenic technology. Heterologous expression of BjCET2 in the double mutant yeast strain Deltazrc1Delt...

journal_title：Plant cell reports

authors： Xu J,Chai T,Zhang Y,Lang M,Han L

更新日期：2009-08-01 00:00:00

abstract：:Two clones of Populus nigra L. were tested in vivo and in vitro for their susceptibility to three different Agrobacterium tumefaciens wild-type strains evaluating number and size of resulting calluses. Strain C58 proved to be the most virulent.Various parameters affecting Agrobacterium-mediated transformation of P. ni...

journal_title：Plant cell reports

authors： Confalonieri M,Balestrazzi A,Bisoffi S

更新日期：1994-02-01 00:00:00

abstract：: St. John's wort (Hypericum perforatum. cv 'Anthos') is a medicinal plant with evidence of efficacy as an anti-depressant. The present report describes the development of an in vitro regeneration system that utilizes thidiazuron [N-phenyl-N'-(1,2,3-thidiazol-yl)urea] for the induction of de novo shoots on etiolated hy...

journal_title：Plant cell reports

authors： Murch SJ,Choffe KL,Victor JMR,Slimmon TY,KrishnaRaj S,Saxena PK

更新日期：2000-05-01 00:00:00

abstract：:A novel member of the WRKY gene family, designated TcWRKY53, was isolated from a cadmium (Cd)-treated Thlaspi caerulescens cDNA library by differential screening. WRKY proteins specifically bind to W-boxes, which are found in the promoters of many genes involved in defense and response to environmental stress. TcWRKY5...

journal_title：Plant cell reports

authors： Wei W,Zhang Y,Han L,Guan Z,Chai T

更新日期：2008-04-01 00:00:00

abstract：KEY MESSAGE:Our studies indicate a potential important elicitor candidate which can aid in the fight against a worldwide disease, rice blast. In this study, we report the purification, identification, characterization, and gene cloning of a novel hypersensitive response-inducing protein elicitor (MoHrip2) secreted from...

journal_title：Plant cell reports

authors： Chen M,Zhang C,Zi Q,Qiu D,Liu W,Zeng H

更新日期：2014-11-01 00:00:00

abstract：:Sugarcane white leaf (SCWL)-diseased sugarcane plants collected from Udornthani Province, in north-eastern Thailand, were the source for tissue culture experiments. Explants from axillary buds, meristem tips, and leaves grew optimally in Murashige-Skoog medium containing 0.5 mg/l alpha-naphthaleneacetic acid, 0.5 mg/l...

journal_title：Plant cell reports

authors： Wongkaew P,Fletcher J

更新日期：2004-11-01 00:00:00

abstract：:The capability of Phalaenopsis to acclimate its photosynthetic capacity and metabolic activity to cool night temperature conditions is crucial for improving orchid production in terms of efficient greenhouse heating. The extent to which Phalaenopsis possesses acclimation potential and the mechanistic background of the...

journal_title：Plant cell reports

authors： Pollet B,Vanhaecke L,Dambre P,Lootens P,Steppe K

更新日期：2011-06-01 00:00:00

abstract：KEY MESSAGE:An ERF gene, LchERF , was cloned from L. chinense for the first time. Overexpression of LchERF conferred salt stress tolerance in transgenic tobacco lines during seed germination and vegetative growth. Ethylene-responsive transcription factors (ERFs) play important roles in tolerance to biotic and abiotic s...

journal_title：Plant cell reports

authors： Wu D,Ji J,Wang G,Guan C,Jin C

更新日期：2014-12-01 00:00:00

abstract：:Reliable regeneration protocols for Eucalyptus camaldulensis using leaf explants from in vitro-grown plants have been developed. Out of the 24 clones tested 13 were regenerated and of these, 6 showed regeneration from more than 60% of the explants. Identical protocols were also successful in the regeneration of some c...

journal_title：Plant cell reports

authors： Mullins KV,Llewellyn DJ,Hartney VJ,Strauss S,Dennis ES

更新日期：1997-09-01 00:00:00