Selection of large quantities of embryogenic calli from indica rice seeds for production of fertile transgenic plants using the biolistic method.

Abstract:

:The microprojectile bombardment of immature embryos has proven to be effective in transforming many indica rice varieties. One of the drawbacks of using immature embryos is the requirement of a large number of high quality immature embryos, which itself is a tedious and laborious process. To circumvent these problems, we have developed a procedure, using indica variety TN1 as a model that generates highly homogenous populations of embryogenic subcultured calli by selectively propagating a small number of regeneration-proficient calli derived from seeds. Thousands of embryogenic calli were produced from 50 seeds within 10 weeks. Ten to 20 independent R0 transgenic lines were regenerated per 500 embryogenic calli bombarded. The convenience and reliability offered by this transformation system has made transformation of indica rice a routine procedure.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Sivamani E,Shen P,Opalka N,Beachy RN,Fauquet CM

doi

10.1007/BF00232364

subject

Has Abstract

pub_date

1996-01-01 00:00:00

pages

322-7

issue

5

eissn

0721-7714

issn

1432-203X

journal_volume

15

pub_type

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