Abstract:
:Site-specific recombination has been developed into a genetic engineering tool for higher eukaryotes. The manipulation of newly introduced DNA is now possible in the course of genetic transformation procedures, thus making the process more predictable and reliable. Also, a wide variety of chromosomal rearrangements using site-specific recombination have been documented both in metazoan and plant species. Applying such methods to plants opens new avenues for large-scale chromosome engineering in the future.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Lyznik LA,Gordon-Kamm WJ,Tao Ydoi
10.1007/s00299-003-0616-7keywords:
subject
Has Abstractpub_date
2003-06-01 00:00:00pages
925-32issue
10eissn
0721-7714issn
1432-203Xjournal_volume
21pub_type
杂志文章,评审abstract::Processes involved in transformation of regenerable soybean (Glycine max (L.) Merrill) immature zygotic cotyledons were studied by assaying the transient expression of the β-glucuronidase gene driven by the 35S promoter and terminated at the 3' end by the soybean 7S storage protein gene. The plasmid containing the chi...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00234510
更新日期:1994-07-01 00:00:00
abstract::Surface-sterilized leaf disks of horse-radish (Armoracia lapathifolia) were immersed in a suspension of Agrobacterium rhizogenes harboring the root-inducing plasmid (pRi) and cultured on a solid medium. Within about 10 days after inoculation, adventitious roots (hairy roots) emerged from the leaf disks. No roots emerg...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00271999
更新日期:1987-07-01 00:00:00
abstract::An in vitro propagation technique based on axillary bud proliferation has been developed for matureSapium sebiferum trees. Nodal segments cultured on Murashige and Skoog (MS) medium supplemented with benzyl adenine (1-10 μM and α-naphthaleneacetic acid (0-0.5 μM showed axillary bud proliferation. Shoots proliferated i...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF01275506
更新日期:1997-06-01 00:00:00
abstract::While searching for genes expressed in acid lemon but not in acidless lime pulp, we isolated clone Cl111 which showed the following expression phenotypes: (1) while it was expressed in the ovaries in both varieties, its mRNA was detected only in the pulp of the acid fruit, (2) no or very low expression of the gene was...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1073-3
更新日期:2011-09-01 00:00:00
abstract::The growth and sweet diterpene glucosides of Stevia plants propagated by stem-tip cultures were compared with those of the control plants propagated by seeds. There was no significant difference between the two groups both in growth and in chemical composition. As for the contents of sweet diterpene glucosides, howeve...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00270194
更新日期:1984-10-01 00:00:00
abstract:: Salvia miltiorrhiza contains two groups of biologically active secondary metabolites termed phenolic compounds (e.g. rosmarinic acid) and tanshinones (e.g. cryptotanshinone). Their roles in plant defense responses were examined using a simplified system consisting of a yeast elicitor and a Ti C58 transformed S. milti...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002999900166
更新日期:2000-06-01 00:00:00
abstract::We have developed a plant- Escherichia coli pASV shuttle vector from the essential elements of the Ageratum yellow vein virus (AYVV). The geminivirus vector contains the AYVV genome with the coat-protein deletion, the E. coli vector backbone of pUC19, a unique cloning site and gene expression cassettes for plant selec...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-004-0792-0
更新日期:2004-08-01 00:00:00
abstract::Low initiation frequency is one of the main barriers in applying somatic embryogenesis to the clonal production of Pinus species. Factors affecting initiation, including basal medium, plant growth regulators, and Phytagel concentration, have been investigated in loblolly pine (Pinus taeda L.). BM1 basal medium proved ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050396
更新日期:1998-02-01 00:00:00
abstract::Tef [Eragrostis tef (Zucc.) Trotter] is the most important cereal in Ethiopia. In its wild relative E. mexicana, regeneration of six green plants resulted from culture of 121 non-pollinated immature pistils. In the allotetraploid crop species tef, however, only callus and root formation was obtained by this method. By...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-006-0200-z
更新日期:2006-12-01 00:00:00
abstract::Polyclonal antibodies against a part of pea (Pisum sativum L.) LOXG protein have been raised to study the pattern of distribution of related lipoxygenases in pea carpels. The antiserum recognized three lipoxygenase polypeptides in carpels. One of them became undetectable 24 hours after fruit development induction, sug...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232465
更新日期:1996-04-01 00:00:00
abstract::Rice plants (Oryza sativa L., Chinsurah Boro II var. Indica) were regenerated from protoplasts isolated from microspore derived cell suspensions. A simple procedure for the establishment of such cell suspension cultures from embryogenic microcallus derived from cultured isolated microspores of Indica-type rice is desc...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232295
更新日期:1990-09-01 00:00:00
abstract::The present protocol was aimed at establishing a routine transformation procedure via Agrobacterium tumefaciens for an important Oncidium orchid cultivar. An expression vector containing hptII and gusA genes driven by the cauliflower mosaic virus (CaMV) 35S promoter was successfully introduced into the Oncidium orchid...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0614-9
更新日期:2003-06-01 00:00:00
abstract::A genetic test was performed on seeds from 283 transgenic tobacco plants obtained by T-DNA transformation. Seeds from self-fertilized transgenic plants were germinated on kanamycin-containing medium, and the percentage of seeds which germinated, as well as the ratio of kanamycin-resistant to kanamycin-sensitive seedli...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00272760
更新日期:1988-12-01 00:00:00
abstract::Approximately 10(6) protoplast-derived cell colonies of sainfoin were stressed with streptomycin and two resistant colonies were recovered. Plants regenerated from these colonies could be recallused on streptomycin-containing medium three years after growth in the absence of the antibiotic.Ultrastructural studies show...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269636
更新日期:1986-12-01 00:00:00
abstract:KEY MESSAGE:A chalcone synthase gene ( PaCHS ) was isolated and functionally characterized from liverwort. The ectopic expression of PaCHS in Marchantia paleacea callus raised the flavonoids content. Chalcone synthase (CHS; EC 2.3.1.74) is pivotal for the biosynthesis of flavonoid and anthocyanin pigments in plants. It...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-014-1702-8
更新日期:2015-02-01 00:00:00
abstract::Leaf, rhizome, and stem explants of Helianthus smithii, a wild relative of the cultivated sunflower, have been tested for their morphogenic potential on media containing a range of hormonal combinations including α-naphthaleneacetic acid and 6-benzylaminopurine (0-2 mg/l). Somatic embryos appeared on the majority of t...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050303
更新日期:1997-07-01 00:00:00
abstract:KEY MESSAGE:The oil palm EgPAL1 gene promoter and its regulatory region were functional as a promoter in the heterologous system of Arabidopsis according to the cis-acting elements present in that region. The promoter was developmentally regulated, vascular tissue specific and responsive to water stress agents. Phenyla...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-017-2228-7
更新日期:2018-02-01 00:00:00
abstract:KEY MESSAGE:Rice and chickpea GDPD s are transcriptionally influenced by mineral deficiencies; especially, by phosphate starvation and CaGDP1 encodes an active glycerophosphodiester phosphodiesterase enzyme. Glycerophosphodiester phosphodiesterases (GDPDs) are enzymes involved in the degradation of glycerophosphodieste...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-016-1984-0
更新日期:2016-08-01 00:00:00
abstract::A procedure to produce fruits from cultured shoot tips of Cucumis sativus L. cultivar 'Marketmore-76' in vitro is described. Four-week-old shoot tips, derived from sterile germinated seedlings on a MS medium, were cultured in a 3.8-1 Mason jar using an automated plant culture system. Tips readily generated roots, leav...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232312
更新日期:1993-11-01 00:00:00
abstract:: The potential of ultrasonic standing waves for trapping suspended particles was utilized successfully for differential size fractionation of plant somatic embryos. In a flow-through resonator equipped with a 200-kHz piezoceramic transducer, embryos of different sizes corresponding to different developmental stages co...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990000234
更新日期:2000-11-01 00:00:00
abstract:KEY MESSAGE:Prolamin-GFP fusion proteins, expressed under the control of native prolamin promoters, were localized in specific layers of PB-Is. Prolamin-GFP fusion proteins were gradually digested from outside by pepsin digestion. In rice seed endosperm, protein body type I (PB-I) has a layered structure consisting of ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-016-1960-8
更新日期:2016-06-01 00:00:00
abstract::The DNA-binding domain of the yeast transcriptional activator GAL4 was expressed in transgenic tobacco plants in order to attempt to obtain specific repression of reporter genes. Expression of the GUS and the NPTII gene was essentially the same regardless of the presence of a gene encoding a functional GAL4 DNA-bindin...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232920
更新日期:1995-10-01 00:00:00
abstract:KEY MESSAGE:TaUBA functions as a negative regulator of salt and drought stress response in transgenic Arabidopsis, either the UBA domain or the zinc finger domain is crucial for TaUBA's function. TaUBA (DQ211935), which is a UBA domain-containing protein in wheat, was cloned and functionally characterized. Southern blo...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-015-1739-3
更新日期:2015-05-01 00:00:00
abstract::KEY MESSAGE : Two soybean cultivars showed markedly different drought tolerance. G6PDH plays a central role in the process of H ( 2 ) O ( 2 ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-012-1374-1
更新日期:2013-03-01 00:00:00
abstract::The expression of three genes that encode proteins involved in peroxisome biogenesis, beta-oxidation and the glyoxylate cycle was studied in Arabidopsis plants by fusing their promoter regions to the reporter gene luciferase. Malate synthase showed an extremely restricted pattern of expression, being detected only in ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-004-0879-7
更新日期:2005-02-01 00:00:00
abstract::A T-DNA insertional mutant OsPE of rice gives twin and triplet seedlings in up to 20 % of the seeds. Detailed cytological and histological analysis of OsPE indicated normal male and female gametogenesis in the OsPE mutant. Confocal laser scanning microscopic (CLSM) analysis of the developing seeds of OsPE showed multi...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-012-1291-3
更新日期:2012-10-01 00:00:00
abstract::A genetic transformation protocol for green ash (Fraxinus pennsylvanica) hypocotyl explants was developed. Green ash hypocotyls were transformed using Agrobacterium tumefaciens strain EHA105 harboring binary vector pq35GR containing the neomycin phosphotransferase (nptII) and beta-glucuronidase (GUS) fusion gene, and ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-009-0697-z
更新日期:2009-06-01 00:00:00
abstract::The introgression of new desirable characters is very necessary in common bean (Phaseolus vulgaris L.)Interspecific hybridization of P. vulgaris L. with P. angustissimus A. Gray, a wild species with narrow leaves, could be successfully achieved for the first time using embryo culture. Sixteen to twenty-three-day old e...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00268593
更新日期:1986-10-01 00:00:00
abstract::Human growth hormone (hGH), a pituitary-derived polypeptide, evidences a wide range of biological functions, including protein synthesis, cell proliferation, and metabolism. A synthetic hGH gene (shGH) has been synthesized on the basis of plant-optimized codon usage via an overlap PCR strategy and located in a plant e...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-008-0514-0
更新日期:2008-05-01 00:00:00
abstract::The growth of Distichlis spicata suspension cultures in LS medium without NaCl was inhibited 54% by 2 mM proline. In medium containing 260 mM NaCl, 10 mM proline inhibited growth by only 22%. The uptake and metabolism of 10 mM L-[1-(13)C] proline was followed by (13)C NMR and ninhydrin analyses of suspensions cultured...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269924
更新日期:1988-08-01 00:00:00