Abstract:
:Protoplasts isolated from mesophyll cells of Eruca sativa Lam., cultured on suitable medium, underwent sustained cell divisions to form calli. The plating efficiency was found to be 0.4%. The protoplast-derived calli subsequently produced plantlets through organogenesis (15.71%) and somatic embryogenesis (11.25%). Regenerated plants exhibited normal appearance. These results indicate potential to introgress desirable traits from this wild crucifer into important oilseed and cole Brassicas by protoplast fusion and hybrid recovery.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Sikdar SR,Chatterjee G,Das S,Sen SKdoi
10.1007/BF00272790subject
Has Abstractpub_date
1987-12-01 00:00:00pages
486-9issue
6eissn
0721-7714issn
1432-203Xjournal_volume
6pub_type
杂志文章abstract::The ability to germinate orchids from seeds in vitro presents a useful and viable method for the propagation of valuable germplasm, maintaining the genetic heterogeneity inherent in seeds. Given the ornamental and medicinal importance of many species within the genus Dendrobium, this review explores in vitro technique...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-015-1829-2
更新日期:2015-10-01 00:00:00
abstract::Protoplasts of sugarbeet (Beta vulgaris L.) were isolated from cell suspension cultures and cultured in modified PGo medium. Conditions required for the efficient division of the protoplasts were investigated.The optimal combination of phytohormones was found to be 1 mg/l NAA, 0.2 mg/l 2,4-D, 0.5 mg/l zeatin. Protopla...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269287
更新日期:1985-08-01 00:00:00
abstract:KEY MESSAGE:The dynamic alterations of the physiological and molecular processes in reproductive stage soybean indicated the dramatic impact caused by drought. Drought is a major abiotic stress that limits soybean (Glycine max) production. Most prior studies were focused on either model species or crops that are at the...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-018-2332-3
更新日期:2018-12-01 00:00:00
abstract::Carotenoid cleavage dioxygenases (CCDs) are involved in the production of diverse apocarotenoids including phytohormones, the visual molecules and the aromatic volatile compounds derived from carotenoids. Here, we examined the spatial expression of four of the CCD genes (AtCcd1, 4, 7 and 8) among the nine members of t...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-010-0999-1
更新日期:2011-06-01 00:00:00
abstract::We report here the successful establishment of callus, cell and root cultures from explants of in-vitro-grown plantlets of the soapwort Saponaria officinalis L. The production of saporin in the different tissue systems was evaluated by determining the capability of crude extracts to inactivate protein synthesis and by...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050351
更新日期:1997-11-01 00:00:00
abstract::A novel genic male sterile (GMS) line in Brassica napus L., which was identified in 1999, was found to be controlled by a monogenic dominant gene, which we have designated as MDGMS. The microspores of the MDGMS abort before the degradation of the tapetal cell layer. The F1 fertility from any fertile lines crossed with...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-006-0276-5
更新日期:2007-05-01 00:00:00
abstract::Quantitative real-time polymerase chain reaction (PCR) assays were designed that enabled the zygosity of transgenes in soybean [Glycine max (L.) Merrill] and peanut (Arachis hypogaea L.) to be determined. The two zygosity assays, based on TaqMan technology that uses a fluorogenic probe which hybridizes to a PCR target...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990100326
更新日期:2001-07-01 00:00:00
abstract:: The influences of light conditions, sucrose and ethylene on in vitro formation and storability of onion (Allium cepa L.) bulblets were studied in various accessions. Light, sucrose and ethylene influenced bulb formation. Storability was primarily enhanced by a high sucrose concentration (100 g/l) in the culture mediu...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990000295
更新日期:2001-02-01 00:00:00
abstract::This study aimed at establishing a protocol to increase the number of regenerated shoots and to limit the recovery of "escapes" during the regeneration of transgenic flax plants (cv Barbara). Here, we describe how light, adapted media and selection scheme could stimulate the transformation process, the organogenic pot...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-008-0638-2
更新日期:2009-03-01 00:00:00
abstract::Separation of stem proteins by sodium dodecyl sulfate polyacrylamide gel electrophoresis permitted classification of 17 celery varieties (Apium graveolens L.) into 7 groups. Several of these groups could be further subdivided into 11 subgroups with gel electrophoresis patterns of 4 isozyme systems. Classification from...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00276666
更新日期:1987-04-01 00:00:00
abstract::Pyrolysis mass spectrometry (PyMS) is a rapid, simple, high-resolution analytical method based on thermal degradation of complex material in a vacuum and has been widely applied to the discrimination of closely related microbial strains. Leaf samples of six species and one variety of higher plants (Rosa multiflora, R....
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0714-6
更新日期:2004-02-01 00:00:00
abstract:: Embryogenic soybean [Glycine max (L.) Merrill.] suspension cultures were bombarded with five different gene constructions encoding the jellyfish (Aequorea victoria) green fluorescent protein (GFP). These constructions had altered codon usage compared to the native GFP gene and mutations that increased the solubility ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050702
更新日期:1999-11-01 00:00:00
abstract::Recently, five novel fluorescent proteins have been isolated from non-bioluminescent species of reef-coral organisms and have been made available through ClonTech. They are AmCyan, AsRed, DsRed, ZsGreen and ZsYellow. These proteins are valuable as reporters for transformation because they do not require a substrate or...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0690-x
更新日期:2003-11-01 00:00:00
abstract::Med8, a subunit of mediator complex, has proved to possess crucial functions in many organisms from yeast to human. In plant, the med8 mutant of Arabidopsis thaliana displayed delayed anthesis and increased number of leaves during the vegetative period. However, the roles of Med8 in other flowering plants are still un...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1118-7
更新日期:2011-11-01 00:00:00
abstract::The cauliflower mosaic virus 35S (35S) and the enhanced 35S (E35S) promoters fused with maize alcohol dehydrogenase (Adh1) intron1 or maize shrunken locus (sh1) intronl along with maize Adh1 and rice actin (Act1) promoters fused to their respective first introns were tested for transient expression of the E.coli β-glu...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00236096
更新日期:1993-07-01 00:00:00
abstract:KEY MESSAGE:Sugarcane lines transformed with an alanine aminotransferase gene demonstrated an improved nitrogen use efficiency compared with untransformed controls in a pot trial under low nitrogen conditions. ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-015-1768-y
更新日期:2015-05-01 00:00:00
abstract:KEY MESSAGE:Overexpression of Withania somnifera SGT gene (WssgtL3.1) in transgenic Arabidopsis improves various agronomic and physiological traits and alters conjugated sterol levels to mitigate the effect of salt stress. Sterols are essential constituents of cell membranes that are involved in several biological func...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-021-02666-9
更新日期:2021-02-01 00:00:00
abstract::A collection of 29 pathogenesis-related 10 (PR10) genes of Medicago sativa and Medicago truncatula showed that they were almost all obtained from cDNA libraries of tissues undergoing abiotic or biotic stresses. The predicted proteins could be divided into two subclasses, PR10.1 and PR10.2, but in silico predicted mode...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-010-0909-6
更新日期:2010-11-01 00:00:00
abstract::Cell suspension cultures of cowpea (Vigna sp.) were able to produce extracellular peroxidase. Different growth regulator concentrations induced different peroxidase activity in callus. The crude extracellular medium after four weeks of culture showed higher (6 times) specific peroxidase activity and higher thermo stab...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232092
更新日期:1990-07-01 00:00:00
abstract::Quaternary benzophenanthridine alkaloids (sanguinarine, chelerythrine, chelirubine, chelilutine and macarpine) are specifically induced by cell wall components of Penicillium and Saccharomyces in a colorless strain of Eschscholtzia californica cell suspension cultures. Classical elicitors such as the Phytophthora mega...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00272770
更新日期:1987-12-01 00:00:00
abstract::Shoot and leaf segments of a non-regenerable Medicago sativa L. genotype were cocultivated with the "shooty" mutant of Agrobacterium tumefaciens carrying the pGV 2206 plasmid. Transformed callus lines were selected and regenerated on the hormone free B5 medium. Southern blot analysis demonstrated integration of T-DNA ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269987
更新日期:1990-04-01 00:00:00
abstract::Callus cultures and cell suspension cultures derived from Ginkgo biloba L. leaves produced ginkgolidc B. In cell suspension cultures, the production reached a maximum by the 13th day of subculture and followed by a sharp decrease. The medium of Murashige and Skoog induced the highest ginkgolide B content in cultures w...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232783
更新日期:1995-05-01 00:00:00
abstract::Cultivation of Catharanthus roseus hairy root cultures at different temperatures was found to have an effect on growth rate and indole alkaloid content as well as lipid composition. When lowering the temperature, the roots responded by increasing the degree of unsaturation of cellular lipids, which was mainly due to a...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00234368
更新日期:1992-07-01 00:00:00
abstract:KEY MESSAGE:Overexpressing TaUb2 promoted stem growth and resulted in early flowering in transgenic tobacco plants. Ubiquitin are involved in the production, metabolism and proper function of gibberellin. The ubiquitin-26S proteasome system (UPS), in which ubiquitin (Ub) functions as a marker, is a post-translational r...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-012-1331-z
更新日期:2012-12-01 00:00:00
abstract:KEY MESSAGE:We describe a steroid-inducible BABY BOOM system that improves plant regeneration in Arabidopsis leaf cultures and yields fertile plants. Regeneration of Arabidopsis thaliana plants for extended periods of time in tissue culture may result in sterile plants. We report here a novel approach for A. thaliana r...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-015-1832-7
更新日期:2015-10-01 00:00:00
abstract::Processes involved in transformation of regenerable soybean (Glycine max (L.) Merrill) immature zygotic cotyledons were studied by assaying the transient expression of the β-glucuronidase gene driven by the 35S promoter and terminated at the 3' end by the soybean 7S storage protein gene. The plasmid containing the chi...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00234510
更新日期:1994-07-01 00:00:00
abstract::We have isolated by affinity chromatography a lectin from the seeds of the winged bean (Psophocrapus tetragonolobus) which agglutinated human (group A, B and O), sheep and rabbit, but not mouse erythrocytes. A molecular weight of 41,000 was obtained from gel filtration, and on sodium dodecyl sulphate polyacrylamide ge...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269646
更新日期:1986-12-01 00:00:00
abstract::Tillering is an important trait of cereal crops that optimizes plant architecture for maximum yield. Teosinte Branched 1 (TB1) is a negative regulator of lateral branching and an inducer of female inflorescence formation in Zea mays (maize). Recent studies indicate that TB1 homologs in Oryza sativa (rice), Sorghum bic...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1139-2
更新日期:2012-01-01 00:00:00
abstract:KEY MESSAGE:SnYSL3 encodes a plasma-localized transporter delivering various metal-nicotianamine complexes. The expression of SnYSL3 is up-regulated by excess Cd, suggesting an important role for SnYSL3 in response to Cd stress. The Yellow Stripe-Like (YSL) transporters have been proposed to participate in metal uptake...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-016-2079-7
更新日期:2017-02-01 00:00:00
abstract::A DNA fragment encoding a 12-amino acid (aa) HIV-1 Tat transduction peptide fused to a 90-aa murine rotavirus NSP4 enterotoxin protein (Tat-NSP4(90)) was transferred to Solanum tuberosum by Agrobacterium tumefaciens-mediated transformation. The fusion gene was detected in the genomic DNA of transformed plant leaf tiss...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0697-3
更新日期:2004-01-01 00:00:00