Regeneration of plants from mesophyll protoplasts of the wild crucifer Eruca sativa Lam.

Abstract:

:Protoplasts isolated from mesophyll cells of Eruca sativa Lam., cultured on suitable medium, underwent sustained cell divisions to form calli. The plating efficiency was found to be 0.4%. The protoplast-derived calli subsequently produced plantlets through organogenesis (15.71%) and somatic embryogenesis (11.25%). Regenerated plants exhibited normal appearance. These results indicate potential to introgress desirable traits from this wild crucifer into important oilseed and cole Brassicas by protoplast fusion and hybrid recovery.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Sikdar SR,Chatterjee G,Das S,Sen SK

doi

10.1007/BF00272790

subject

Has Abstract

pub_date

1987-12-01 00:00:00

pages

486-9

issue

6

eissn

0721-7714

issn

1432-203X

journal_volume

6

pub_type

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