Genetic transformation of Medicago species by Agrobacterium tumefaciens and electroporation of protoplasts.

abstract：:The capability of Phalaenopsis to acclimate its photosynthetic capacity and metabolic activity to cool night temperature conditions is crucial for improving orchid production in terms of efficient greenhouse heating. The extent to which Phalaenopsis possesses acclimation potential and the mechanistic background of the...

journal_title：Plant cell reports

authors： Pollet B,Vanhaecke L,Dambre P,Lootens P,Steppe K

更新日期：2011-06-01 00:00:00

abstract：:Domain of Unknown Function 966 (DUF966) gene family was found in the protein family database, which consisted of seven genes in rice. The proteins encoded by these genes contained one or two highly conserved DUF966 domains. The available data of public microarray databases implied that these genes might play crucial r...

journal_title：Plant cell reports

authors： Luo C,Guo C,Wang W,Wang L,Chen L

更新日期：2014-02-01 00:00:00

abstract：KEY MESSAGE:The cytokinin pathway promotes the initiation of bulbil formation, and iPA may an important type of cytokinin during bulbil formation in Lilium lancifolium. Bulbils are important vegetative reproductive organs in triploid Lilium lancifolium. We previously showed that cytokinins are involved in bulbil format...

journal_title：Plant cell reports

authors： He G,Yang P,Tang Y,Cao Y,Qi X,Xu L,Ming J

更新日期：2020-07-01 00:00:00

abstract：: Cells of a suspension culture of Doritaenopsis cv. New Toyohashi were placed in a mixture of 2 M glycerol and 0.4 M sucrose for 15 min at room temperature and then dehydrated with a vitrification solution (PVS2) for 1-3 h on ice and plunged into liquid nitrogen. The highest viability (64% by 2,3,5-triphenyltetrazoliu...

journal_title：Plant cell reports

authors： Tsukazaki H,Mii M,Tokuhara K,Ishikawa K

更新日期：2000-12-01 00:00:00

abstract：:Microcallus (more than 60 cells) formation was obtained from leaf mesophyll protoplasts of 6 species and varieties in the genus Actinidia Lindl. (kiwifruit). The best results were achieved by using liquid over agarose culture for A. arguta var. arguta, liquid and agarose disc type culture for A. arguta var. purpurea, ...

journal_title：Plant cell reports

authors： Xiao XG,Hirsch AM

更新日期：1996-09-01 00:00:00

abstract：:The steroidal hormone brassinosteroids (BRs) play important roles in plant growth and development. Genetic, genomic and proteomic studies in Arabidopsis have identified major BR signaling components and elucidated the signal transduction pathway from the cell surface receptor kinase BRI1 to the BES1/BZR1 family of tra...

journal_title：Plant cell reports

authors： Hao J,Yin Y,Fei SZ

更新日期：2013-07-01 00:00:00

abstract：:A protocol for whole plant regeneration of Cicer arietinum L. cv. C-235 via organogenesis from callus has been developed. Callus initiation was best when immature leaflets were cultured on MS medium containing 5 or 25 μM 2,4-D or NAA in combination with 10 μM BA, or 25 μM 2,4-D alone. The callus grew most vigorously o...

journal_title：Plant cell reports

authors： Barna KS,Wakhlu AK

更新日期：1994-06-01 00:00:00

abstract：:The study used Actinidia deliciosa endosperm-derived callus to investigate aspects of the morphology, histology and chemistry of extracellular matrix (ECM) structures in morphogenically stable tissue from long-term culture. SEM showed ECM as a membranous layer or reticulated fibrillar and granular structure linking th...

journal_title：Plant cell reports

authors： Popielarska-Konieczna M,Kozieradzka-Kiszkurno M,Swierczyńska J,Góralski G,Slesak H,Bohdanowicz J

更新日期：2008-07-01 00:00:00

abstract：:A friable and fast-growing type of callus was isolated from a long term shoot-competent cell culture of wheat. The suspension cultures established from this callus consisted of small, densely cytoplasmic cells which divided more rapidly but with a lower plant regeneration frequency than the original culture. A high yi...

journal_title：Plant cell reports

authors： Chang YF,Wang WC,Warfield CY,Nguyen HT,Wong JR

更新日期：1991-03-01 00:00:00

abstract：KEY MESSAGE:Thirty genes involved in GA and ABA metabolism and signalling were identified, and the expression profiles indicated that they play crucial roles in the bud activity-dormancy transition in tea plants. Gibberellin (GA) and abscisic acid (ABA) are fundamental phytohormones that extensively regulate plant grow...

journal_title：Plant cell reports

authors： Yue C,Cao H,Hao X,Zeng J,Qian W,Guo Y,Ye N,Yang Y,Wang X

更新日期：2018-03-01 00:00:00

abstract：:The variation among and within different populations of the regeneration ability from leaf, cotyledon and hypocotyl explants has been studied. A control population and two lines selected by their regeneration capacity from leaf explants were used. Significant differences among the plants of the control population,for ...

journal_title：Plant cell reports

authors： Molina RV,Nuez F

更新日期：1995-01-01 00:00:00

abstract：:The effects of serial combinations of either indole-3-acetic acid, indole-3-butyric acid or α-naphthaleneacetic acid (0.5-10.0 mg/l) with either kinetin, 6-benzyl-amino-purine, zeatin or 6-methylaminopurine (0.5-5.0 mg/l) have been investigated to assess the morphogenetic potential of foliar explants of Duboisia myopo...

journal_title：Plant cell reports

authors： Kukreja AK,Mathur AK,Ahuja PS

更新日期：1986-02-01 00:00:00

abstract：:Leaf mesophyll protoplasts isolated from pea (Pisum sativum L.) genotypes Century and PI244253 showed transient expression of β-glucuronidase (GUS) when electroporated with plasmid DNA containing various promoter-leader sequence constructs driving the GUS gene. The optimum conditions for transient expression were: usi...

journal_title：Plant cell reports

authors： Hobbs SL,Jackson JA,Baliski DS,Delong CM,Mahon JD

更新日期：1990-06-01 00:00:00

abstract：:Leaf, rhizome, and stem explants of Helianthus smithii, a wild relative of the cultivated sunflower, have been tested for their morphogenic potential on media containing a range of hormonal combinations including α-naphthaleneacetic acid and 6-benzylaminopurine (0-2 mg/l). Somatic embryos appeared on the majority of t...

journal_title：Plant cell reports

authors： Laparra H,Stoeva P,Ivanov P,Hahne G

更新日期：1997-07-01 00:00:00

abstract：:Ethylene emanation rates were assessed from leaf tissues of an embryogenic seed plant (Cycle 0) and regeneration cycle plants selected for enhanced embryogenesis (Cycles I, II and IV). In all experiments, ethylene was assessed from the basal 1 cm portion of the innermost leaf. Ethylene emanation was five-fold higher i...

journal_title：Plant cell reports

authors： Songstad DD,Petracek PD,Sams CE,Conger BV

更新日期：1989-03-01 00:00:00

abstract：:A system to regenerate fertile rice (Oryza sativa L.) plants (both indica and japonica varieties) from protoplasts isolated from anther-derived embryogenic haploid suspension cultures has been established. Green plants were regenerated from protoplast-derived cell clusters five months after suspension culture initiati...

journal_title：Plant cell reports

authors： Su RC,Rudert ML,Hodges TK

更新日期：1992-12-01 00:00:00

abstract：:Tissue culture methods for improvement of cotton has lagged seriously compared to other major crops. A method for regeneration of cotton which includes a morphogenetically competent cell suspension was needed to facilitate selection of stress-resistant variants and gene manipulation. Preliminary screening of eight str...

journal_title：Plant cell reports

authors： Trolinder NL,Goodin JR

更新日期：1987-06-01 00:00:00

abstract：:An early identification of fusion products was based on the presumed vigorous growth of hybrid calluses after fusion between Solanum brevidens and S. tuberosum leaf protoplasts. The S. brevidens protoplasts were unable to form multicellular colonies under the applied culture conditions. Three size groups of calluses w...

journal_title：Plant cell reports

authors： Polgár Z,Preiszner J,Dudits D,Fehér A

更新日期：1993-05-01 00:00:00

abstract：KEY MESSAGE:The zeaxanthin epoxidase gene ( MsZEP ) was cloned and characterized from alfalfa and validated for its function of tolerance toward drought and salt stresses by heterologous expression in Nicotiana tabacum. Zeaxanthin epoxidase (ZEP) plays important roles in plant response to various environment stresses d...

journal_title：Plant cell reports

authors： Zhang Z,Wang Y,Chang L,Zhang T,An J,Liu Y,Cao Y,Zhao X,Sha X,Hu T,Yang P

更新日期：2016-02-01 00:00:00

abstract：:The aim of this study was the improvement of embryo production in wheat anther culture. Three butanol alcohols, n-butanol, sec-butanol and tert-butanol, were evaluated for their effect on microspore embryogenesis in two spring cultivars of wheat, Pavon and Caramba. Application of n-butanol, at 0.1 and 0.2% (v/v) in th...

journal_title：Plant cell reports

authors： Soriano M,Cistué L,Castillo AM

更新日期：2008-05-01 00:00:00

abstract：:We have developed a plant- Escherichia coli pASV shuttle vector from the essential elements of the Ageratum yellow vein virus (AYVV). The geminivirus vector contains the AYVV genome with the coat-protein deletion, the E. coli vector backbone of pUC19, a unique cloning site and gene expression cassettes for plant selec...

journal_title：Plant cell reports

authors： Tamilselvi D,Anand G,Swarup S

更新日期：2004-08-01 00:00:00

abstract：:The effect of various inhibitors on differentiation (shoot morphogenesis) in callus cultures of Brassica, Datura and Nicotiana has been investigated. Hormone medium without any inhibitor (control), resulted in 6% shoot formation. Addition of inhibitors such as actinomycin D, cordycepin, abscisic acid, trigonelline and...

journal_title：Plant cell reports

authors： Sethi U,Basu A,Mukherjee SG

更新日期：1990-03-01 00:00:00

abstract：:An efficient micropropagation system for Saussurea involucrata Kar. et Kir., an endangered Chinese medicinal plant, has been developed. Shoot organogenesis occurred from S. involucrata leaf explants inoculated on medium with appropriate supplements of plant growth regulators. 66.0% of shoot regeneration frequency and ...

journal_title：Plant cell reports

authors： Guo B,Gao M,Liu CZ

更新日期：2007-03-01 00:00:00

abstract：KEY MESSAGE:The first Good Manufacturing Practices production of a purification-free rice-based oral cholera vaccine (MucoRice-CTB) from transgenic plants in a closed cultivation system yielded a product meeting regulatory requirements. Despite our knowledge of their advantages, plant-based vaccines remain unavailable ...

journal_title：Plant cell reports

authors： Kashima K,Yuki Y,Mejima M,Kurokawa S,Suzuki Y,Minakawa S,Takeyama N,Fukuyama Y,Azegami T,Tanimoto T,Kuroda M,Tamura M,Gomi Y,Kiyono H

更新日期：2016-03-01 00:00:00

abstract：: An Agrobacterium tumefaciens-mediated transformation method has been developed for onions (Allium cepa L.) using immature embryos as the explant source. Transgenic plants were recovered from the open-pollinated onion cultivar Canterbury Longkeeper at a maximum transformation frequency from immature embryos of 2.7%. T...

journal_title：Plant cell reports

authors： Eady CC,Weld RJ,Lister CE

更新日期：2000-03-01 00:00:00

abstract：:Responses induced by Cd exposure were assessed in tobacco seedlings (Nicotiana tabacum L.) using macro and molecular indices. The 100 microM of Cd exposure reduced the total dry weight and chlorophyll index of the seedlings as much as the genuine Fe-deficiency. Concentration of Fe in the shoots decreased, whereas that...

journal_title：Plant cell reports

authors： Yoshihara T,Hodoshima H,Miyano Y,Shoji K,Shimada H,Goto F

更新日期：2006-04-01 00:00:00

abstract：:A protocol is described for rapid and large-scale propagation of the woody aromatic and medicinal shrub Vitex negundo by in vitro culture of nodal segments from mature plants. Of the three different cytokinins - N6-benzyladenine (BA), kinetin, and thidiazuron - evaluated as supplements to Murashige and Skoog (MS) medi...

journal_title：Plant cell reports

authors： Sahoo Y,Chand PK

更新日期：1998-12-01 00:00:00

abstract：KEY MESSAGE:By comparing series full-length cDNA libraries stressed and control, the dynamic process of salt stress response in Upland cotton was studied, and reactive oxygen species and gibberellins signaling pathways were proposed. The Upland cotton is the most important fiber plant with highly salt tolerance. Howeve...

journal_title：Plant cell reports

authors： Shi G,Guo X,Guo J,Liu L,Hua J

更新日期：2015-06-01 00:00:00

abstract：:From the in vitro hairy root cultures of a Datura candida hybrid, 19 tropane alkaloids have been identified using capillary gas-liquid chromatography and mass spectrometry. As in the parent plants, scopolamine is the major alkaloid. Two hitherto undescribed alkaloids have been detected and their structure tentatively ...

journal_title：Plant cell reports

authors： Christen P,Roberts MF,David Phillipson J,Evans WC

更新日期：1990-07-01 00:00:00

abstract：:Rhodamine-6G(R6G), a lipophilic dye which degrades mammalian mitochondria, was shown to arrest the division of Nicotiana protoplasts. When albino recipient-protoplasts were treated with R6G and fused with X-irradiated (green) donor- protoplasts, only green cybrid plants were obtained. The mtDNA of the cybrids was anal...

journal_title：Plant cell reports

authors： Aviv D,Chen R,Galun E

更新日期：1986-06-01 00:00:00